巻号一覧
39 巻, 4 号
選択された号の論文の9件中1～9を表示しています
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I. ESTIMATION OF EFFECTIVE POPULATION SIZE OF THE KISO NATIVE HORSESKEN NOZAWA, TAKESHI TOMITA, KOZABURO ESAKI1964 年 39 巻 4 号 p. 199-208
発行日: 1964年
公開日: 2007/05/21
ジャーナル フリーThe authors, together with other co-workers, have already estimated the average effective population size of the Kiso horses in a period from 1955 to 1961 as about 90 per year from the number of male parents and the variability in progeny number of male parents. In the present work the average effective size of this horse population was estimated from the random fluctuation of the frequencies of genes controlling polymorphism for coat-colors and red blood cell antigens by using a method developed by one of the authors (Nozawa 1963). The value obtained, 88.51 (57.78-152.59) per year, was considered to agree with the estimate from the breeding record.抄録全体を表示PDF形式でダウンロード (826K) -
XXII. ON TUMOR-DAMAGING ACTION OF CHROMOMYCIN A3 (TOYOMYCIN, TAKEDA)YANAGI KIMURA1964 年 39 巻 4 号 p. 209-216
発行日: 1964年
公開日: 2007/05/21
ジャーナル フリーDamaging effects of an antibiotic, Chromomycin A3 (Toyomycin, TAKEDA) were cytologically examined on tumor cells of the MTK-sarcoma III (an ascites sarcoma of white rats), HeLa cells and human embryonic cells.
Chromomycin A3 exerted severe damaging effects on tumor cells in vivo and in vitro as well as on embryonic cells in vitro: the pycnotic disintegration of resting nuclei and the depression of DNA substances were remarkable under treatment.
Remarkable prolongation of life span was observed by single application of this agent at dose level of 20μg/100g, and in some cases by repeated injections at daily dose of 10μg/100g body weight.抄録全体を表示PDF形式でダウンロード (1514K) -
TAIRA KATAYAMA1964 年 39 巻 4 号 p. 217-221
発行日: 1964年
公開日: 2007/05/21
ジャーナル フリー1. It was found from a cytological observation that the giant cells formed in a completely sterile rice plant induced by X-rays are syncytes and not coenocytes.
2. There was not observed any morphological difference that distingushed the syncyte-forming and normal plants.
3. Twenty-four chromosomes were counted in the somatic cells of the sterile plant.
4. The cell fusion to form syncytes was found to have started already at the early stage of meiosis.
5. Number of nuclei per PMC varied from 0 to 21.
6. Chromosome configulations per nucleus varied from 8II+8I to 69II+3I.
7. The muximum chromosome number counted was about 220.
8. No data on the genetic behavior of syncyte formation were obtained because of the complete sterility of this plant.
9. There was a great variation in the size of pollen grains.抄録全体を表示PDF形式でダウンロード (1000K) -
TOSHITERU MORITA1964 年 39 巻 4 号 p. 222-239
発行日: 1964年
公開日: 2007/05/21
ジャーナル フリーAn eye color mutant of Drosophila melanogaster, ry, contains no uric acid at any developmental stage, but accumulates a large amount of hypoxanthine compared with the amount of xanthine at pupal and imaginal stages.
An enzyme concerned with uric acid production in D. melanogaster of the wild type is a xanthine dehydrogenase, which has both the activities of xanthine oxidation and DPNH oxidation. The xanthine dehydrogenase from ry mutant, however, does not have xanthine oxidative activity, but retains the activity of DPNH oxidation. The enzymatic activity of DPNH oxidation in ry is at the same level as in the wild strain. The xanthine oxidative activity in the enzyme from heterozygous strain, ry/+, is one-half of the activity in the wild strain, but the DPNH oxidative activity is at the same level as in a wild strain.
These results suggest that xanthine dehydrogenase produced in the ry mutant is an enzyme molecule lacking the active site of xanthine oxidation.
The activity of guanase, which catalyzes the conversion of guanine to xanthine, is observed in pupal and imaginal stages of both wild and ry strains. From these results, it is shown that xanthine, which is a precursor of uric acid, is derived not only from hypoxanthine with dehydrogenase but also from guanine with guanase.抄録全体を表示PDF形式でダウンロード (1383K) -
SOTA HIRAGA1964 年 39 巻 4 号 p. 240-253
発行日: 1964年
公開日: 2007/05/21
ジャーナル フリーTen eye-color mutants (ge and its allele ge2, ocra and its allele ocrac, w57, do, car, cm, rb and bu) of the housefly, Musca domestica L., were studied for tryptophan metabolism.
1) A great amount of accumulation of tryptophan was observed in pupae of the ge mutant, while neither kynurenine nor 3 OH-kynurenine were detected. No xanthommatin exists in eyes of adults of this mutant. Therefore, ge is a mutant blocked at the first step of tryptophan metabolism. It was proved that ge and ge2 have no activity of tryptophan pyrrolase in cell-free extracts.
2) In pupae of ocra and ocrac mutants, an excessive amount of kynurenine was accumulated, but 3 OH-kynurenine was undetectable. Thus, these are mutants whose activities of kynurenine hydroxylase are genetically blocked. Xanthommatin was undetected in eyes of adults of ocra. In ocrac, however, it was proved that a little amount of the pigment was accumulated (8 to 10% of the wild type). The ocrac may be a leaky mutant of kynurenine hydroxylase.
3) In do (dark-orange), tryptophan accumulated in 5 times the amount of that in the wild-type pupae, while accumulations of kynurenine and 3 OH-kynurenine were normally observed. Enzymatic activity of over-all conversion from tryptophan to kynurenine in the cell-free extract of this mutant was half of the wild-type.
4) It is likely that ge and ocra mutants of the housefly are homologous to the v and cn mutants of D. melanogaster, respectively.
5) Separation of tryptophan pyrrolase from kynurenine formamidase was obtained by gradient elution chromatography on a DEAF-column.抄録全体を表示PDF形式でダウンロード (1127K) -
AKIO KUSANAGI1964 年 39 巻 4 号 p. 254-258
発行日: 1964年
公開日: 2007/05/21
ジャーナル フリーRNA synthesis during mitosis was studied by autoradiographic techniques in the root meristematic cells of Luzula purpurea (n=3).
Comparisons of the synthetic activity of the interphase with those of other mitotic phases were made using whole nuclear grain counts.
Incorporation of uridine-H3 in chromosomes decreased during early-prophase and completely stopped at mid-prophase. Incorporation of uridine-H3 in nucleoli was retained until late-prophase, just before nucleolar disintegration. No incorporation of uridine-H3 was observed in meta-anaphase chromosomes. Incorporation of uridine-H3 into the daughter chromosomal groups began at early-telophase and continued until late-telophase.
Pre-nucleolar material appeared on the surface of each chromosome as early as in late-anaphase before RNA synthesis resumed in daughter nuclei. Nucleolus developed in parallel with an increase of the incorporation of uridine-H3 in chromosomes and nucleolus itself.抄録全体を表示PDF形式でダウンロード (436K) -
I. THE DEVELOPMENT OF EGGS TRANSPLANTED WITH IRRADIATED NUCLEI OR CYTOPLASMHAJIME SAMBUICHI1964 年 39 巻 4 号 p. 259-267
発行日: 1964年
公開日: 2007/05/21
ジャーナル フリーFrog eggs γ-irradiated before insemination developed normally during the early stages, and then most of them became abnormal embryos. From the remainings a few metamorphosed haploid frogs and a few non-viable haploid tadpoles were obtained.
The transfers in which the cytoplasm had been irradiated and the nucleus was introduced from a normal blastula cell were very low in developmental capacity, as compared with the control transfers whose cytoplasm and nucleus had not been irradiated. However, two metamorphosed frogs and a tadpole were obtained from among rN transfers.
All the transfers in which the cytoplasm was normal and the nucleus was introduced from an irradiated blastula cell did not develop beyond the heart-beat stage, as irradiated blastulae did not.抄録全体を表示PDF形式でダウンロード (1357K) -
瀬戸 武司1964 年 39 巻 4 号 p. 268-275
発行日: 1964年
公開日: 2007/05/21
ジャーナル フリー本文は冷血動物の組織培養の特殊性についてのべ, とくに両生類を用いた場合の培養方法を詳述した。
培養に用いる材料としては, 無尾両生類では腎臓, 有尾両生類では肺臓の組織が in vitro で最もよい増殖を示すことから, 組織培養の材料としては好適である。両生類の培養を成功せしめるためには, 1) 体外移植前に組織の滅菌を充分に行なう, 2) 組織片が培養器内で容易にガラス壁に附着できるような処理を施す, 3) 両生類用の培養液を用い, pH を7.4以下7.0までの範囲内にとどめる, 4) 培養温度を25～26°Cに保つことに特に留意する必要がある。
冷血動物の血液を培養して白血球を増殖させることは恒温動物の血液培養と同様可能であり, 両生類の白血球培養法についても併せ記した。抄録全体を表示PDF形式でダウンロード (1450K) -
東海林 隆次郎, 木村 やなぎ1964 年 39 巻 4 号 p. 276-282
発行日: 1964年
公開日: 2007/05/21
ジャーナル フリーHeLa 細胞の一般的形態および染色体におよぼす Endoxan の影響を May-Grünwald Giemsa 染色法および空気乾燥法を用いて細胞学的に観察した。
細胞の増殖初期 (継代培養直後) に投与した場合には, 対照に比較していちぢるしい増殖抑制効果が見られ, それは処理濃度および処理時間に比例して増大した。細胞の増殖最盛期 (培養後48-72時間目) に投与した場合には増殖抑制効果はほとんど観察されなかった。
また, in vivo の実験において高頻度で観察されるという放射線類似の分裂異常は本実験に関するかぎりほとんど認められなかった。抄録全体を表示PDF形式でダウンロード (1069K)
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