The Japanese Journal of Genetics

Buffer soluble seed proteins of 20 ditelocentric lines of a common wheat, Triticum aestivum cv. Chinese Spring, were analyzed by gel electrofocusing. Result clearly showed that the short arms of chromosome 3A and 3B, and the β arm of chromosome 3D are responsible for the respective formation of three fractions, bands 3, 4 and 7. These chromosome arms all had a common effect on a diffused band between bands 1 and 2. This provides additional evidence for the genetic homoeology between 3A, 3B and 3D chromosomes. Of the other chromosomes, chromosomes 4A, 6A, 1B and 4B showed some qualitative effects on one or two bands.

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Oxazolidinethione, one of the main goitrogenic substances in the seed meals of various cultivars of Cruciferous species such as B. napus, B. campestris, B. oleracea, B. nigra, B. juncea and Raphanus sativus, was measured spectrophotometrically and was calculated as l-5-vinyl-2-oxazolidinethione. The number of cultivars examined in each species was 22, 40, 22, 1, 4 and 2, respectively.
No oxazolidinethione was found in the seed meals of B. nigra, B. juncea and R. sativus, confirming the results of previous workers.
Mean value of oxazolidinethione content of seed meals from napus cultivars was 10.09±2.3mg per gram of dry meals, with a range from 14.6 to 3.7mg. Low content cultivars seemed to belong to those derived from interspecific crosses between B. napus and B. campestris.
B. campestris cultivars yielded slightly or not at all, however all forage turnips yielded about 1.0mg, which are commonly supposed to be originated from spontaneous interspecific hybrids between rutabaga (B. napus) and turnip (B. campestris).
B. oleracea varied in oxazolidinethione content and mean value was 2.37±2.44mg, with a range from 9.5 to 0.0mg.
It seemed that b-genome had not genetic factors producing the oxazolidinethiones and the source of the factors was mainly in c-genome.
Though it must not be easy to breed the napus cultivars containing no or a small amount of oxazolidinethione, there should be an assured possibility to synthesize such cultivars by interspecific crosses between selected cultivars of B. campestris and B. oleracea.

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