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Volume 48 (1973)
- Issue 6 Pages 391-
- Issue 5 Pages 315-
- Issue 4 Pages 247-
- Issue 3 Pages 159-
- Issue 2 Pages 79-
- Issue 1 Pages 1-
Volume 48, Issue 2
Displaying 1-9 of 9 articles from this issue
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MUNEO IIZUKA, ERNESTO MATSUMOTO, AKIRA DOI, REMIGIO MADRIGAL, AKIRA FU ...1973 Volume 48 Issue 2 Pages 79-87
Published: 1973
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSCallus formation and organ differentiation were studied in tubular florets cultured in vitro of chrysanthemum and cineraria. The basal medium was the one modified from Murashige and Skoog (1962). Callus formation and organ differentiation varied according to combinations and concentrations of growth substances. One of the best media for plantlet induction contained 10-7M 2, 4D in combination with IBA, IAA and 6-BAR of each 10-5M. Cultivar differences in callus differentiation were observed. One cultivar exhibited mainly root differentiation and other cultivars produced leaves and stems. 6-BAR pretreatment for cut shoot promoted callus development of florets and stimulated root differentiation in medium containing only IBA or IAA. Plantlets differentiated from callus subcultured in a medium containing 10-5M each of IAA, 6-BAR and inosine and lacking 2, 4D. However, though callus development was promoted, no differentiation of organs was observed from callus subcultured in a medium containing 10-5M each of 2, 4D and 6-BAR. However individuals of chrysanthemum derived from culture showed usual chromosome counts, few exceptional variations in leaf shape and flower color had appeared.View full abstractDownload PDF (1335K) -
I. HEREDITY OF THE BLOOD GROUP-ACTIVE O ANTIGENS IN SALMONELLA GROUPS G, R, AND UKOICHIRO KISHI, SHOEI ISEKI1973 Volume 48 Issue 2 Pages 89-97
Published: 1973
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSFrom the genetic studies, it has become clear that the specific structures of the O antigens of 13, 22 or 13, 23 (group G) with blood group H activity, 40 (group R) with A, 43 (group U) with B and 4, 12 (group B), that is, the structures of the S-specific side chains, are determined at a gene locus (the O locus) close to the his locus in Salmonella.
Phage P22 (iota) can produce the O antigen 1 only in an organism in which the O antigen 12 is manifested.View full abstractDownload PDF (908K) -
TAKUMA GAMO1973 Volume 48 Issue 2 Pages 99-104
Published: 1973
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSFibroin and sericin extracted from the lumen of the middle division of silkgland in the two mutants, naked pupa (Nd) and sericin-cocoon (Nd-s), were examined on polyacrylamide gel electrophoresis containing 8M urea, and the existence of different components in fibroin and sericin was demonstrated with the reduction of disulphide bonds. Proteins from the larval silkgland of the two mutants contained a small quantity of a fibroin fraction migrating with slower mobility than the light fraction of fibroin from a normal gland. The silkgland of Nd-s mutant contained a large quantity of a rapidly migrating component of sericin which is absent in the secretion from normal glands. However, the sericin extracted from Nd-gland consisted of the similar fractions as obtained from the normal gland.View full abstractDownload PDF (1253K) -
TOSHIHIDE KIKUCHI1973 Volume 48 Issue 2 Pages 105-118
Published: 1973
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSAn olfactory mutant of Drosophila melanogaster has been isolated. In 97 chemicals examined, the mutant is strongly attracted by 18 chemicals repllent to parent strain: These are salicylaldehyde, morin, maltol, kojic acid, methyl acetoacetate, 2, 4-pentanedione, benzoylacetone, 2, 3-butanedione, six carboxylic acids and four aliphatic monoketones. These chemicals except four monoketones contain a molecular feature as the ‘bifunctional unit’ consisting of a proton-acceptor (A) and a proton-donor (D) in gas phase, showing an average A-D distance of about 3Å.View full abstractDownload PDF (1311K) -
SUMIKO NARISE1973 Volume 48 Issue 2 Pages 119-132
Published: 1973
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThirteen electrophoretic variants of esterases in Drosophila virilis, 9 of which controlled by Esterase-α locus and the others by Esterase-β locus respectively, have been partially purified and characterized biochemically. Enzymes purified by column chromatographic techniques from each stock were free from all other esterases on agar gel electrophoresis but contained a little amount of other proteins. α-esterase utilized both of α- and β-naphthyl acetates at the same extent, while β-esterase did not utilized the α-substrate. Both esterases were more strongly inhibited by DFP than by eserine. PCMB inhibited greatly the activity of α-esterase (80.1% by 10-4M) but not that of β-esterase (7.6% by 10-4M). EDTA did not have any effect on either of α- and β-esterases. The inhibition by PCMB of α-esterase was in part due to the action on -SH group involved in the active center. Generally speaking, the optimal pH of α-esterase was slightly lower than that of β esterase. β-esterase was more unstable than α-esterase by the heat treatment at 60°C. Molecular weight of α-esterase was estimated as 75, 000 and that of β-esterase was as 140, 000 by method of Sephadex gel filtration. Isoallelic variants of the esterases were very similar, there being no significant difference among them in substrate specificity and in molecular weight. However, there were differences in inhibition rates by DFP and PCMB, in optimal pH and in resistibility to treatment at 60°C.View full abstractDownload PDF (1992K) -
II. HEREDITY OF THE O ANTIGENS 4, 5 AND 9 OF SALMONELLAKOICHIRO KISHI, SHOEI ISEKI1973 Volume 48 Issue 2 Pages 133-136
Published: 1973
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThe genetic determinants of O antigen 5 of Salmonella group B may be situated near his and not far from met locus, and can express its action only in an organism in which O antigen 4 is also manifested. On the other hand, the genetic determinants of O antigen 4 of Salmonella group B and 9 of Salmonella group D are found to be situated on the O locus near his and to be probably allelic. The genetic determinant of O antigen 5 is at least outside the range of cotransduction by use of P22 (iota) phage with his+ and O-4.View full abstractDownload PDF (404K) -
TAKUMA GAMO1973 Volume 48 Issue 2 Pages 137-145
Published: 1973
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSEffects of temperature during embryonic development upon the manifestation of multistar marking, which appears on the larval integument of the silkworm, were studied using five strains showing different phenotypes. Two kinds of multistar marking controlled by the non-allelic genes, ms-i and ms-d, are discriminated by the effectiveness of low temperature, the manifestation of the phenotype on the 10th segment and the result of breeding experiments. One, ms-i, increases the expressivity at low temperature (15°C) and lacks the star marking on the 10th segment, while the other, ms-d decreases the expressivity and produces the marking on the 10th segment. Temperature is effective for both multistar markings during early embryonic blastokinesis, at which time the dorsal integument of the abdominal segment as well as the anlage of this marking differentiates. Different responses to low temperature in the two genes seem to depend upon the time of gene action; the ms-d gene acts earlier than ms-i. The expressivity of both multistarts was also increased by incubation at 35°C for one day; at this temperature the activity of the suppressors regulating the expressivity of this marking might be inactivated.View full abstractDownload PDF (965K) -
MUNEHICO IWATA1973 Volume 48 Issue 2 Pages 147-149
Published: 1973
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDownload PDF (304K) -
POLYMORPHISM FOR A SUPERNUMERARY SEGMENT IN NATURAL POPULATIONS OF MECOSTETHUS MAGISTER (ORTHOPTERA)HIROSHI KAYANO1973 Volume 48 Issue 2 Pages 151-153
Published: 1973
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDownload PDF (880K)
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