遺伝学雑誌
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
52 巻, 1 号
選択された号の論文の8件中1~8を表示しています
  • TAKAO K. WATANABE, WON HO LEE, YUTAKA INOUE, MASAOKI KAWANISHI
    1977 年 52 巻 1 号 p. 1-8
    発行日: 1977年
    公開日: 2007/05/21
    ジャーナル フリー
    1.The genetic variation of the hybrid crossability between Drosophila melanogaster females and D. simulans males was examined by using eight geographical strains of D. simulans from Japan.
    2. The hybridization was measured by insemination rate, egg to adult viability, and the number of hybrid progeny. The most experiments were undertaken with Oregon-R melanogaster females and various simulans male strains. The variation among simulans strains was clearly detected in these measurements and they were in large part positively correlated with each other.
    3.The temperature sensitivity of the hybrid varied from strain to strain of simulans, and was not ascribed to the geographical origin of the strain.
    4. D. melanogaster females were also variable in the hybrid crossability. Six melanogaster strains were significantly different in the insemination rate. A significant variance due to the interaction between melanogaster×simulans was also detected.
    5. The yellow mutant of melanogaster females showed an excellent receptivity to simulans males, and the yellow locus itself seemed to respond it.
  • TAKAO K. WATANABE, MASA-AKI YAMADA
    1977 年 52 巻 1 号 p. 9-14
    発行日: 1977年
    公開日: 2007/05/21
    ジャーナル フリー
    1. The NSR-spirochetes injected to Drosophila simulans killed sons in the embryonic stage and the SR condition was established in this species by transmission of the spirochetes from mother to daughter through generations.
    2. Inter-species hybrid males between D. melanogaster and D. simulans were killed by the NSR agent mostly at the stages of larvae and pupae, while the hybrid females were not affected.
    3. Daughterless gene (da) killed the hybrid females in the embryonic stage.
  • KEN NOZAWA, TAKAYOSHI SHOTAKE, YOSHIKO OHKURA, YUICHI TANABE
    1977 年 52 巻 1 号 p. 15-30
    発行日: 1977年
    公開日: 2007/05/21
    ジャーナル フリー
    Genetic variability within and between 13 populations of 7 species of the Asian macaques was quantified by using data obtained from electrophoretic examinations of blood proteins. Variations of 28 to 29 genetic loci controlling the structure of 26 to 27 kinds of enzymatic and non-enzymatic blood proteins were examined in a total number of 1, 404 samples. The proportion of polymorphic loci was 14-41% and the mean heterozygosity per individual 1.9-10.8% except in the Yaku Island population of the Japanese macaque (M. fuscata yakui) where the genetic variation was completely lacking. It was considered that the genetic variability in a breeding unit or troop of the taxa examined here would be lower than these values since each sampled population was regarded to be a mixture of individuals from different origins. The genetic distances between different “species” were too small to be considered as real biological species. Recalling the fact that these “species” are completely interfertile and have the same chromosome constitution, the authors consider that a biologically more acceptable system of classification of the Asian macaques could be reconstructed by lowering the so-called “genus” to the rank of species and the so-called “species” to the rank of subspecies.
  • TOSHINOBU MORIKAWA
    1977 年 52 巻 1 号 p. 31-39
    発行日: 1977年
    公開日: 2007/05/21
    ジャーナル フリー
    In order to identify monosomic oats, Avena byzantina C. Koch cv. Kanota, intercrosses to produce 40-chromosome hybrids were made between twenty monosomic lines and cytogenetical characteristics of the hybrids were observed. The results of this experiment clearly showed that several monosomics, such as Mk 3, 4, 6, 9, 13 and 22 produced functional 20-chromosome pollen as well as 21-chromosome pollen. All plants with 40 chromosomes were found to be double monosomics which mostly showed 19"+2' chromosome pairing. It was confirmed that monosomic parents which produced double monosomics (Mk 2 and 3; Mk 1, 2, 3, 4 and 8 and 6; Mk 4, 6 and 13 and 9; Mk 1 and 15 and 13; Mk 20 and 22) were deficient for different chromosomes each other. Of 14 kinds of double monosomics obtained from these intercrosses, three which were deficient for a common chromosome 9, such as 4/9, 6/9 and 13/9 showed considerable differences in seed and pollen fertilities and chromosome configurations from each other. Based on the results, it was concluded that Mk 4, 6 and 13 were deficient for functionally different chromosomes each other. These results also indicated that the comparison for various characteristics between double monosomic lines which were deficient for a common chromosome was the effective method for identification of monosomic oats.
  • KOJIRO KURISU
    1977 年 52 巻 1 号 p. 41-51
    発行日: 1977年
    公開日: 2007/05/21
    ジャーナル フリー
    Tooth germs of first and second molars of both jaws obtained from new born mice of inbred strains were stained with alizarin red S dye and examined under a stereoscopic microscope. For numerical analysis a stage number assigned was converted to the calcification rate using calcium content of samples from a given stage. Although each molar presented a rather distinctive sequence and pattern of calcification, there were considerable variations within strains in several stages. A statistical method to reduce the component of variance due to factors other than age was proposed by the author and used. Curvilinear regression was applied to elucidate the growth curve of calcification of molars. In the examination of bilateral symmetry in calcification, low symmetry was observed at the beginning of spurt of calcification and A/J strain showed relatively lower symmetry than other strains. Sex difference was not observed in the growth of calcification.
  • TADA-AKI HORI
    1977 年 52 巻 1 号 p. 53-64
    発行日: 1977年
    公開日: 2007/05/21
    ジャーナル フリー
    A temperature-sensitive cell cycle variant, ts-1 has been isolated from a pseudodiploid Chinese hamster cell line, D6 after mutagenesis with EMS and cytosine arabinoside selection. The ts-1 grows well at the permissive temperature, 34°C, but not at the non-permissive temperature, 40°C, although some fractions of the cells divide once when shift up the temperature to 40°C. The ts-1 was further examined by autoradiography to determine if any functions at a unique stage in the cell cycle is defective at the non-permissive temperature. The studies on the rates of macromolecular synthesis revealed that the overall rate of DNA synthesis, but not RNA and protein synthesis, was affected at the non-permissive temperature. The cessation of DNA synthesis was mainly due to a decrease in the number of cells synthesizing DNA. These results together with studies on asynchronous and mitotically synchronized cells suggest that the ts-1 is a cell cycle variant defective in a function which is active throughout the entire G1 phase and is required for entry to the S phase, but no longer required once cells enter the S phase.
  • HIDEO TSUJI, HATAO KATO, KAZUO MORIWAKI
    1977 年 52 巻 1 号 p. 65-71
    発行日: 1977年
    公開日: 2007/05/21
    ジャーナル フリー
    Fibroblast cultures established from mouse embryos showed an abnormally high incidence of spontaneous chromosomal aberrations at early passages. The aberration frequency reached a peak (39.5%) at the 4th passage (13 days after initiation of the cultures), and then declined gradually. Culture media collected from the mouse cultures were found to be capable of inducing chromosomal aberrations in Chinese hamster cells. This indicates that some chromosome-damaging substances had evolved in the mouse primary cultures and were secreted into the culture medium. These substances seemed to affect cells very rapidly, since the production of chromosomal aberrations was detected as early as 2h after Chinese hamster cells were exposed to the conditioned medium derived from the mouse cultures. The substances appeared to be heat-labile, as the mouse conditioned medium lost its chromosome-damaging effect when it was prewarmed at 37°C for 24h. The nature of these toxic substances is, however, yet unknown. Preliminary studies on the primary cultures derived from other mammals imply that the phenomenon reported here is specific to certain groups of mammals except primates.
  • YASUMOTO KIKUCHI, SHINYA HITOTSUMACHI, MASAJI NOMURA, TAKESHI NAKAGUCH ...
    1977 年 52 巻 1 号 p. 73-79
    発行日: 1977年
    公開日: 2007/05/21
    ジャーナル フリー
    A phenotypically male Wistar rat having reduced body-weight and small penis is investigated histologically and cytogenetically. Left and right gonads are represented by ovotestes.
    Chromosome studies demonstrate the occurrence of two cell-lines, one line showing 41 chromosomes without the Y and the other having 42 chromosomes with an additional minute chromosome. Morphological and Giemsa-banding analyses indicate that the minute chromosome is resulted from a deletion of the long arm of the Y. Thus, a true hermaphroditism characterized by the 41, X/42, XYq- mosaic complex is established in this rat.
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