Conventional gene analysis, monosomic analysis and telocentric method of mapping were carried out with respect to α-amylase zymogram variations of germinating seed of hexaploid wheat. The gene for Band 1(
Amy 6D1) was located on 6DL with the recombination value of 11.9±2.8(%) from the centromere. Its minor allele,
Amy 6D1M and null allele,
Amy 6D1° were also detected in
spelta duhamelianum, and Prelude, respectively. The locus for Band 2 was polymorphic, there being two alleles,
Amy 6A1 and
Amy 6A1M, for Band 2 major and minor, respectively. The gene for Band 3,
Amy 6B1, was on 6BL with the recombination value of 19.4±3.8(%) from the centromere. On this locus, there were two other alleles,
Amy 6B14′, and
Amy 6B1° for Band 4′ and absence of both Band 3 and 4′, respectively. On the same chromosome was another α-amylase locus having two alleles,
Amy 6B2 for Band 10 and its null allele,
Amy 6B2°, distant from
Amy 6B1 by the recombination value of 20.6±2.2(%). There were two alleles,
Amy 4 and
Amy 4M for Band 4 major and minor.
Amy 7B2 for Band 14 was detected on 7B in addition to
Amy 7B1 for Band 15.
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