巻号一覧
58 巻, 5 号
選択された号の論文の15件中1～15を表示しています
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Takahiro TAGUCHI, Yukimasa SHIRAISHI1983 年 58 巻 5 号 p. 393-403
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーRadiosensitive and resistant cell lines were studied with regard to radiation survival and chromosomal changes. A radioresistant cell clone (CCRF-HSB2-M) was spontaneously separated from a radiosensitive human T-cell line (CCRF-HSB2) which was originated from the peripheral blood of the patient with an acute lymphoblastic leukemia (ALL). Cell growth and dose-survival curves after X-irradiation indicated that HSB2-M cells were as resistant as normal cells, though the frequency of chromosomal aberrations in irradiated HSB2-M cells was slightly higher than that in irradiated normal cells. Higher frequencies of chromatid and chromosome deletions (breaks) were observed in irradiated HSB2 cells, whereas those of deletions in HSB2-M and normal cells showed a relatively low frequency and the chromosomal aberrations in these two cell lines decreased with time. The karyotype of radiosensitive HSB2 cells was XY, 46, t(1;7)(p32;q32). The radioresistant mutant clone (HSB2-M) gained some other changes in addition to the original translocation; 47, XY, t(1;7)(p32;q32), +t(6;6)(p11;q13), t(12;16)(q24;q11), -16p, +22. The acquirement of radioresistant character in HSB2-M cells could be closely related to the additional chromosomal changes (+iso6q and +22). The emergence of a radioresistant clone from a radiosensitive cell line is discussed in relation to human leukemia.抄録全体を表示PDF形式でダウンロード (685K) -
Hiroko IKEDA-KOMATSU, Ryuso TANAKA1983 年 58 巻 5 号 p. 405-410
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーIn the interspecific F1 hybrids of Crepis, C-banding patterns under the condition of amphiplasty were studied. In each hybrid of C. capillaris×C. dioscoridis, C. capillaris×C. vesicaria and its reciprocal cross, C-banding patterns of individual chromosomes of the hybrids were similar to those of the parents, and were unchanged in the F1 hybrids and in the condition of amphiplasty. This fact indicates that the differential and neutral amphiplasties do not give rise to the material change of chromosomes.抄録全体を表示PDF形式でダウンロード (335K) -
I. L-2-a gene in Asian native cultivarsYo-Ichiro SATO, Kisaburo HAYASHI1983 年 58 巻 5 号 p. 411-418
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーThe F1 weakness, found in the crosses of a Peruvian rice cultivar, Jamaica, and Japanese lowland cultivars, is known to be controlled by a set of complementary genes, L-2-a and L-2-b, the latter being carried by Jamaica. Geographical distribution of L-2-a gene was surveyed in the Asian native cultivars. Among 130 Japanese lowland cultivars, 122 proved to carry L-2-a gene. In 55 cultivars from various Asian countries other than Japan, all the cultivars classified as the temperate Japonica, 13, carried this gene. None of 26 Indicas and only one of 16 tropical Japonicas were the carriers of this gene. The posession of L-2-a may be considered as a characteristic of the temperate Japonica type of rice.抄録全体を表示PDF形式でダウンロード (308K) -
Hiroaki ICHIKAWA, Atsushi HIRAI1983 年 58 巻 5 号 p. 419-424
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーChloroplast DNAs (ctDNAs) from Brassica species were analyzed to identify the female parent of B. napus, which is the amphidiploid after the interspecific hybridization between B. oleracea and B. campestris.
The putative parents and their reciprocal hybrids were subjected to ctDNA analysis by Bam HI restriction patterns. The results demonstrated ctDNA from each of the reciprocal hybrids was identical to that from the female parent of the hybrids, showing the maternal inheritance of ctDNA in these particular crosses. B. napus ctDNA was compared with the putative parental ctDNA by Bam HI and Eco RI restriction patterns. The results showed that B. napus ctDNA was indistinguishable with B. oleracea ctDNA and different from B. campestris ctDNA. From above evidences, we concluded that B. oleracea was the female parent of B. napus, although it seems to be very rare to arise the hybrids by cross between _??_, B. oleracea and B. campestris in nature.抄録全体を表示PDF形式でダウンロード (223K) -
Hideki FUKATA, Hirosuke FUKASAWA1983 年 58 巻 5 号 p. 425-432
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーProperties of two DNA topoisomerases isolated from cauliflower inflorescence were further investigated using negatively supercoiled pBR322 DNA and its relaxed form as the substrates. It was revealed that the ATP-independent enzyme (topoisomerase-I) has the capacity to relax negatively or positively supercoiled DNA at the presence or absence of ATP. ATP-dependent enzyme (topoisomerase-II) also removes supercoils from negatively or positively supercoiled DNA at the presence of ATP, but its reaction products are found to have some different electrophoretic behaviour from the products by topoisomerase-I. Prepared substrates with a unique linking number was catalyzed with topoisomerase-I or -II, and electrophoresed, indicating that simultaneous breaking and rejoining of DNA strands by topoisomerase-I and topoisomerase-II take place on a single-strand of the duplex DNA and on doublestrands, respectively.抄録全体を表示PDF形式でダウンロード (367K) -
II. Crossing ability of F1 hybrids and their progeniesNobumichi INOMATA1983 年 58 巻 5 号 p. 433-449
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーCrossing ability was examined in the F1 hybrids of Brassica campestris× B. oleracea, and the character and somatic chromosomes of F2, B1 and the hybrid plants were investigated. From these results, it was suggested that the different types of normal gametes might be produced in the F1 hybrids with 19, 38 and 28 chromosomes. in the F1 hybrids with 19 chromosomes, most normal egg cells had 19 chromosomes which were unreduced gamete and normal pollen grains had 10 chromosomes. In the spontaneous F1 amphidiploids with 38 chromosomes, normal egg cells had 19 chromosomes and normal pollen grains had 10 and 19 chromosomes. In the F1 hybrids with 28 chromosomes, normal egg cells had 10, 19 and 28 chromosomes and normal pollen grains had 10 chromosomes. New type plants of B. napus were obtained from the progenies of three types of the F1 hybrids.抄録全体を表示PDF形式でダウンロード (964K) -
Hiroaki INOUE, Yasuo FUKUMORI, Toshiki HIROYOSHI1983 年 58 巻 5 号 p. 451-461
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーA female determining factor (F), epistatic to M factors, was used to map autosomal male determining factors (AM) of the housefly. On the basis of meiotic recombination frequency occurring in the sex-reversed females caused by F, three IM factors and two IIIM factors of different geographic origins were mapped in the close vicinity of the bp and the pw locus, respectively. These results suggest that AM factors occupy a definite site on the respective chromosomes. As measured along the IM and the IIIM chromosomes, the distribution of recombination in the sex-reversed females was strikingly different from that observed in the male. It is proposed that AM factors are located in the centric heterochromatin.抄録全体を表示PDF形式でダウンロード (480K) -
Etsuko TAKANASHI1983 年 58 巻 5 号 p. 463-485
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーTwelve local populations of Drosophila sulfurigaster albostrigata from the Philippines, Palawan, Borneo, the Malay Peninsula, Thailand and Sri Lanka, were crossed to D. s. bilimbata and D. s. sulfurigaster. With respect to postmating isolation, the fertility and the sex ratio distortion in F1 hybrids and the frequency of the insemination reaction causing the reaction mass were investigated. In crosses to D. s. sulfurigaster, the extent of postmating isolation, both prezygotic and postzygotic ones, were relatively weak. In crosses to D. s. bilimbata, the extent of both kinds of isolation suggested that local populations of D. s. albostrigata have genetically diverged among the six regions described above. In interpopulational hybridization, hybrids from crosses of marginal populations showed the hybrid breakdown in the F2 or F3 generation for egg-to-adult viability. On the other hand, in premating isolation to D, s. neonasuta estimated by no-choice experiments, significant genetic differentiation among local populations of D. s. albostrigata was not detected. In intersubspecific crosses, between two groups; one consists of D. s. neonasuta and D. s. albostrigata and the other D. s. bilimbata and D. s. sulfurigaster, prezygotic isolation by the insemination reaction causing the reaction mass was developed in either of the reciprocal crosses, and postzygotic isolation by F1 male sterility and the sex ratio distortion in F1 progeny in the other crosses. Strong one sided sexual isolation was observed between D. s. neonasuta and the other group including D. s. bilimbata and D. s. sulfurigaster. It is clear that isolating mechanisms to prevent the gene exchange between subspecies have developed to some extent. On the basis of the degree of genetic and reproductive differentiation estimated by hybridization experiments, the processes of subspecies formation in D. sulfurigaster are discussed.抄録全体を表示PDF形式でダウンロード (1024K) -
II. Further studies on geographical differencesKenji MIKASA, Takashi NARISE1983 年 58 巻 5 号 p. 487-496
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーGeographic variation in emigration behavior at various temperatures was examined with newly collected wild strains of Drosophila melanogaster. Natural populations from various geographic regions showed three basic emigration response patterns to temperature: linear, threshold, and optimum response types. A new type of temperature-influenced emigration behavior, valley-shaped response type, was observed in specimen from Tobishima island in Tohoku. The area specific response types were found in geographically different populations. Genotype-environment interaction is an important factor for temperature-influenced emigration behavior. Climatic and island factors affect the emigration response behaviors.抄録全体を表示PDF形式でダウンロード (356K) -
I. Bristle counts and hybridization experimentTakumi HARA, Haruo KUROKAWA1983 年 58 巻 5 号 p. 497-504
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーBristle counts of the sixth sternites of males were performed for four related species of Drosophila auraria complex. Drosophila auraria male has about 14 bristles. Drosophila triauraria and D, quadraria have about 13 and 12 bristles, respectively. Drosophila biauraria has generally none. The difference found in the mean bristle counts was statistically significant between species. Wild type strains of D. auraria and D, biauraria were hybridized and males of the F1 and the backcrossed progenies were compared as concerned with their sternite bristles. It was found that the X chromosome and the autosome (s) were both responsible for the manifestation of the sternite bristles.抄録全体を表示PDF形式でダウンロード (286K) -
Takeo OHNISHI, Yasuhiro OHASHI, Keiichi NOZU, Shozo INOKI1983 年 58 巻 5 号 p. 505-509
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーThe effect of an anti-trypanosomal drug, Ro 7-1051, on killing, mutation induction and prophage induction activities in Escherichia coli has been investigated. A radiation sensitive strain, NG30(recA-), was more sensitive for killing than the wild type strain, H/r30R, and the another radiation-sensitive strain, Hs30R(uvrA-). The killing effect on Hs30R was almost the same as that on H/r30R. Ro 7-1051 induced mutation (arg-→arg+) in both Hs30R and H/r30R strains, while no significant increase of mutation frequency was detected in NG30 strain. The prophage induction was hardly detected from both E, coli W3110(λ) and W3110(λ ind8) strains. The characteristics of lesion of DNA induced by Ro 7-1051 are discussed.抄録全体を表示PDF形式でダウンロード (200K) -
Masatoshi ENOMOTO1983 年 58 巻 5 号 p. 511-517
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーThe location of the Salmonella phase-2 flagellin gene and its associated regulatory elements, vh2+ H2 rh1+ and vh2 H2 rh1+, transduced into Escherichia coli K-12 was determined by interrupted matings to be around 55min on the linkage map. In P1 transduction with vh2 H2 nalBr as donor, tyrA+ or pheA+ was cotransduced with vh2 H2 at frequencies of 3-6% but was not with nalBr, suggesting that the integrated H2 region interferes with cotransduction of pheA and nalB which are known as cotransducible, and also its likely location at the nalB side of pheA. On the other hand, the vh2+ H2 region was not cotransduced with any markers tested and not transduced at all even when used as selective marker. This was discussed with relation to the constitution of the transducing fragment involving vh2+ H2. The delayed expression of H2 in transductants selected for auxotrophic markers was also discussed.抄録全体を表示PDF形式でダウンロード (310K) -
Hideo YAMAGATA, Takanori SATO1983 年 58 巻 5 号 p. 519-523
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーSucrose-dependent, spectinomycin-resistant mutations of Escherichia coli responsible for alterations in cytoplasmic membrane and ribosomal proteins were analyzed genetically using a series of λ transducing phages carrying E. coli deoxyribonucleic acid of various lengths from the aroE-rpsL region. Mutations of seven mutants were mapped in three different regions of the ribosomal gene cluster. No spectinomycin-resistance mutation had been mapped previously in these three regions.抄録全体を表示PDF形式でダウンロード (199K) -
VI. An autosomal, recessive, non-maternal effect female-specific lethal mutantYoshie INOUCHI, Tadanobu OKUNO, Kugao OISHI, Takao K. WATANABE1983 年 58 巻 5 号 p. 525-530
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーA recessive, non-maternal effect, female-specific lethal mutant of Drosophila melanogaster was recovered from a natural population. The mutant, fle(3)100, is located at 3-45±. About one-half of homozygous females show normal development up to the pharate adult stage. Salivary gland chromosomes of third instar homozygous female larvae have normal staining properties in the lactic-aceto-orcein staining, suggesting normal dosage compensation. The results indicate that the fle(3)100 gene forms a new class of sex-specific lethals.抄録全体を表示PDF形式でダウンロード (244K) -
Soichi TANDA, Samuel H. HORI1983 年 58 巻 5 号 p. 531-537
発行日: 1983年
公開日: 2006/07/25
ジャーナル フリーWe have previously reported that the major regulatory factor responsible for an increase of glucose-6-phosphate dehydrogenase in Drosophila melanogaster may not be linked to any of chromosome 1, 2 and 3 (Tanda and Hori 1982, 1983). Recently, an X-linked mutation that gives rise to flies of very high enzyme activity was found in some progenies of a strain which formerly had the factor unlinked to the X chromosome. This mutation appears to be due to insertion of the regulatory factor into the X chromosome.
Several strains which were so constructed as to be homozygous for the X chromosomes of male mutants exhibited high enzyme activities at generation 0 without exception, but decreases in enzyme activity occurred in the succeeding generations. Reasoning that such decreases might be due to excision of the regulatory factor or its translocation to somewhere else in the genome where it becomes silent, X chromosomes were extracted from males showing high and low enzyme activities, and their isogenic progenies were assayed for enzyme activity. The results showed that low-activity males produced low-activity progenies, whereas high-activity males produced high-activity progenies, thus suggesting that the sampled males were hemizygous for an X chromosome with or without the functional regulatory factor.
In view of these and other findings, the possibility may be envisaged that there may be an extrachromosomal factor in the nuclei of D. melanogaster which is capable of stimulating the G6PD activity, and could occasionally be inserted into the X chromosome.抄録全体を表示PDF形式でダウンロード (293K)
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