The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
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Volume 59, Issue 6
Displaying 1-5 of 5 articles from this issue
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  • Variation of the number of first spermatocytes in relation to fertility in Drosophila virilis
    Yuzuru OGUMA, Haruo KUROKAWA, Yoshio SOGAWA
    1984 Volume 59 Issue 6 Pages 565-575
    Published: 1984
    Released on J-STAGE: July 25, 2006
    DOIhttps://doi.org/10.1266/jjg.59.565
    JOURNAL FREE ACCESS
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    The number of first spermatocytes per cyst in 5 iso-female strains of Drosophila virilis was studied with regard to fertility. The cysts involving 8cells were dominantly seen in TK and B33, and 9cells in A12 and A13 strains. But B12 strain had a highest mode of 12cells. Meioses could be assumed to proceed in every strain. The analysis of variance for the number of spermatids per bundle showed great difference between B12 and any other strain. The male fertility was also significantly different between TK and B12. The mean progeny numbers per male were 2300.1 in TK and 243.1 in B12, respectively. No significant difference was detected in female fertility.
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  • Chromosomal localization of the rat albumin gene in normal and analbuminemic rats determined by in situ hybridization
    Kenji SUGIYAMA, Sumi NAGASE, Tosihide H. YOSIDA
    1984 Volume 59 Issue 6 Pages 577-583
    Published: 1984
    Released on J-STAGE: July 25, 2006
    DOIhttps://doi.org/10.1266/jjg.59.577
    JOURNAL FREE ACCESS
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    Recombinant plasmids containing rat albumin cDNA were radiolabeled and hybridized in situ to metaphase chromosome preparations of Wistar strain rats. Results showed that in a significantly high percentage of the hybridized cells (27%) silver grains were located on the distal portion of chromosome 2: silver grains on this portion amounted to 17% of all the chromosomal label. Thus the albumin gene locus is mapped on chromosome 2. Therefore, the sixth linkage group, including the gene loci of albumin, the plasma protein pattern (Gl-1) and a coat color (h), is probably located on chromosome 2. The chromosomal location of the albumin gene was examined in an albumin-deficient strain of Sprague-Dawley rats. Of the silver grains, 18% were located on the distal region of chromosome 2. These findings support the previous conclusion that the albumin gene is not deleted from analbuminemic rats, and also indicate that the albumin gene locus is not displaced in these rats.
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  • Induction of triploid carp and their haematological characteristics
    Koichi UENO
    1984 Volume 59 Issue 6 Pages 585-591
    Published: 1984
    Released on J-STAGE: July 25, 2006
    DOIhttps://doi.org/10.1266/jjg.59.585
    JOURNAL FREE ACCESS
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    The triploids of carp (Cyprinus carpio) have been induced at a maximum frequency of 91.7% by the cold-shock treatment of the eggs shortly after fertilization. The erythrocytes and their nuclei of the triploids had changed to long ellipsoids that had grown more in the major axis than the minor axis, and with a surface area of 1.44 and 1.40times, respectively, than that of the diploids. The number of erythrocytes of the triploids had decreased to 0.6 times than that of the diploids.
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  • Induction of prophage λ in ultraviolet light sensitive
    Hiroaki IYEHARA OGAWA, Nozomu OTSUJI
    1984 Volume 59 Issue 6 Pages 593-599
    Published: 1984
    Released on J-STAGE: July 25, 2006
    DOIhttps://doi.org/10.1266/jjg.59.593
    JOURNAL FREE ACCESS
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    Induction of prophage λ in ultraviolet light sensitive ruv mutants of Escherichia coli K-12 was studied. When the ruv+ thy (λ) strain was starved for thymine, a maximum induction occurred after about 150min, whereas induction of the ruv thy(λ) strains reached the same level at about 90min. The number of infective centers in a ruv+tif (λ) strain reached a maximum at 60min after the temperature was raised to 42°C, whereas a maximum induction was achieved at 45min in the ruv tif (λ) strains. These results suggest that the ruv mutations affect the process of prophage induction in thy auxotrophs and in tif strains.
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  • Relative durations of the cell cycle phases of Xenopus cells at different temperatures
    Abdulaziz A. AL-SALEH
    1984 Volume 59 Issue 6 Pages 601-606
    Published: 1984
    Released on J-STAGE: July 25, 2006
    DOIhttps://doi.org/10.1266/jjg.59.601
    JOURNAL FREE ACCESS
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    It has been proved that the duration of each phase in cell cycle of Xenopus cells can easily be determined by counting cells in G1, S, G2 and M in autoradiographed and stained preparations, and multiplying the fraction of cell number in each phase with generation time (Tg). At some different incubation temperatures, the following results were obtained: (1) at 18°C, G1, S, G2 and M were 30.2h, 29.5h, 10.1h and 2.2h with the total generation time of 72h; (2) at 23°C, G1, S, G2 and M were 15.1h, 16.2, 4.3h and 0.4h with the total generation time of 36h; and (3) at 28°C, G1, S, G2 and M were 12h, 13.8h, 3.9h and 0.3h with the total generation time of 30h.
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