遺伝学雑誌
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
64 巻, 3 号
選択された号の論文の6件中1~6を表示しています
  • I. Reproductive isolations and the phylogeny
    Bong Ki KIM, Takao K. WATANABE, Osamu KITAGAWA
    1989 年 64 巻 3 号 p. 177-190
    発行日: 1989年
    公開日: 2006/07/25
    ジャーナル フリー
    Seventeen species of the Drosophila montium subgroup, originated from the Southeast Asia, were genetically examined to clarify the phylogenetic relationships. Among 272 interspecific crosses, 61 combinations were successful in mating and 39 combinations produced hybrid flies. These results enabled us toclassify the subgroup into three species complexes: the kikkawai complex (6 species), the jambulina complex (4 species) and the auraria complex (7 species), which were very similar to that obtained by the electrophoretic classification (Ohnishi and Watanabe, 1984). Asymmetrical mating preference between species was found in the present experiment. They were applied to estimate the relative age of species according to the hypothesis proposed by Watanabe and Kawanishi (1979). The evolving order was as follows: kikkawai, leontia, pennae, lini-like, lini, bocki in the kikkawai complex, punjabiensis, punjabiensis-like, jambulina, barbarae in the jambulina complex, and quadraria, yuwanensis, rufa, subauraria, biauraria, triauraria, auraria in the auraria complex. Hybrid flies, if produced, were mostly fertile or partially fertile in both sexes (32/39) within species complex crosses. Therefore premating isolation played a more important role than postmating isolation in speciation of the D. montium subgroup.
  • Kazuhiko NODA, Koulin GE
    1989 年 64 巻 3 号 p. 191-198
    発行日: 1989年
    公開日: 2006/07/25
    ジャーナル フリー
    Tetraploid wheats were divided into the Emmer (genomic constitution, AABB) and the Timopheevi (AAGG) groups based on the low level of meiotic chromosome pairing and sterility in their hybrids. Triticum dicocoides lines KU8821A and KU8821C and T. araraticum line KU8821B, which show a high level of meiotic chromosome pairing in the hybrids, have been considered to be intermediate types between the Emmer and the Timopheevi wheats. These intermediate types may play an important role in the differentiation of tetraploid wheats. Chromosome structural differentiation was examined by the N-banding technique. These lines showed either the banding patterns of the Timopheevi or of the Emmer groups, and were not intermediate plants between the two groups based on the chromosome structure. The present results suggest that the Emmer and the Timopheevi groups were derived diphyletically in the evolution of tetraploid wheats.
  • Kenji TANIGUCHI, Ryuso TANAKA
    1989 年 64 巻 3 号 p. 199-208
    発行日: 1989年
    公開日: 2006/07/25
    ジャーナル フリー
    Shoot primordia were induced from suspended cells derived from Crepis capillaris calli, and were regenerated into shoots. For the induction of suspended cells, the calli were transferred into a liquid MS medium containing 2mg/l NAA on a rotary shaker at 100rpm under the continuous illumination of 2, 000lux. The suspended cells developed shoot primordia through transferring into a liquid 1/2 MS basal medium containing 0.02mg/l NAA, 0.2mg/l BAP and 30g/l sucrose on a rotary shaker at 100 rpm under the continuous illumination of 2, 000lux. In order to proliferate the shoot primordia, they were transferred into a liquid B5 medium containing 1.0mg/l NAA and 0.5mg/l BAP on a gyratory shaker at 2rpm under 10, 000lux illuminated continuously by a halogen lamp. The shoot primordia regenerated plantlets on a B5 agar medium containing 0.2mg/l BAP and 10g/l sucrose. Chromosomes of suspended cells and the shoot primordia cells were examined. Seventeen percent of the suspended cells examined showed 2n=6 chromosomes with normal karyotype, and the others were karyologically abnormal. The masses of shoot primordia induced from the suspended cells were composed respectively of cells with normal, structurally changed, or duplicated karyotype. It was confirmed that the respective masses of shoot primordia were able to maintain clonally as masses of homogeneous cells with respective karyotype.
  • Hymenoptera, Vespidae
    Hidehiro HOSHIBA, Makoto MATSUURA, Hirotami T. IMAI
    1989 年 64 巻 3 号 p. 209-222
    発行日: 1989年
    公開日: 2006/07/25
    ジャーナル フリー
    The chromosomes of 21 species of Vespidae (consisting of 9 polistines and 12 vespines) were compared using an air-drying technique. The haploid number of the Polistinae ranged broadly (n=14, 22, 23, 26, 30, 31, and 34), while it was either n=25 or n=26 in the Vespinae. There were no karyological homologies (in terms of marker chromosomes) between these two subfamilies. Some phylogenetic relationships of social wasps were discussed, based on the karyological literature now available (32 species). We suggested that the three genera of Vespinae (Dolichovespula, Vespa, and Vespula) would have differentiated in two different ways from a common ancestor having 27 acrocentrics (n=27) by tandem fusion (the first genus with n=26) and by two centric fusions (the latter two genera with n=25). In the genus Polistes, the 5 subgenera (Fuscopolistes, Aphanilopterus, Megapolistes, Polistella, and Polistes) would have evolved independently toward increasing chromosome numbers since they started to separate from their ancestor having n=9-14.
  • Yoko NISHIZAWA, Atsushi HIRAI
    1989 年 64 巻 3 号 p. 223-229
    発行日: 1989年
    公開日: 2006/07/25
    ジャーナル フリー
    The genes for the β and ε subunits of ATP synthase were cloned from rice chloroplast DNA. The nucleotide sequences and the deduced amino acid sequences of these genes were determined. The site of initiation of transcription was examined by S1-mapping analysis. The relation between stability of mRNA and that of a stem and loop structure at the 3′ end of mRNA is discussed.
  • Masamichi NAKAMURA, Masaki ITO, Kiyoshi FUJITA, Yasuyuki TAKAGI
    1989 年 64 巻 3 号 p. 231-238
    発行日: 1989年
    公開日: 2006/07/25
    ジャーナル フリー
    A mutant strain (ttr-3) of Escherichia coli was originally isolated as a strain resistant to tributyltin exhibiting temperature-sensitive depressions of growth and ATP synthesis on succinate plates at 42°C. The ttr gene was mapped between the pyrE and dnaA genes (in the 82-83 min region) on the chromosome by P1-transduction experiments. Comparison of proline transport and oxygen uptake by membrance vesicles of the wild-type transductant and the mutant (ttr-3) transductant showed that membrane vesicles of the mutant exhibited temperature-sensitive decrease of proline transport and increase of oxygen uptake at the restrictive temperature (42°C), compatible with depression of growth of the mutant at this temperature. Therefore, the ttr gene seems to code for some factor involved in the respiratory chain that is present in the inner membrane of Escherichia coli.
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