巻号一覧
70 巻, 4 号
選択された号の論文の8件中1～8を表示しています
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Ling Zhi XIAO, Sadao ICHIKAWA1995 年 70 巻 4 号 p. 473-485
発行日: 1995年
公開日: 2004/07/02
ジャーナル フリーMutagenic interactions between maleic hydrazide (MH; a promutagen known to be activated into a mutagen in plant cells) and X rays were studied in the stamen hairs of Tradescantia clone BNL 4430, a blue/pink heterozygote. The young inflorescence-bearing shoots with roots cultivated in the nutrient solution circulating growth chamber were used as tester plants. After determining doseresponse curves for X rays and for MH, nine combined treatments with MH (0.5 and 1 mM) and X rays (292 to 1,240 mGy) were conducted, exposing to X rays either 20 or 44 h before, at the midpoint of, or 2 or 44 h after the MH treatments for 4 h. Clear synergistic effects in inducing somatic pink mutations were detected when X rays were given before the MH treatments. On the contrary, however, antagonistic effects were often observed when X-ray treatments were carried out during or after the MH treatments. The synergistic effects detected were thought to be the results of interactions between DNA strand breaks (and the resultant chromosomal breaks) induced by X rays and those by MH, whereas the antagonistic effects observed were presumed to have resulted from X-ray-caused inhibition of the activation of MH in the stamen-hair cells.
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Vesna STOJILJKOVIC, Mirjana MILANOVIC, Mirjana MILOSEVIC, Marko ANDJEL ...1995 年 70 巻 4 号 p. 487-495
発行日: 1995年
公開日: 2004/07/02
ジャーナル フリーExperiments were designed to estimate the variation of alpha-amylase activity in Drosophila subobscura individuals homozygous for AmyS and AmyF alleles at the Amy locus. The measurements of enzyme activities in six groups of male progenies determined in each individual have shown that degree of variation differs between S and F strains. Variability of amylase activity among male progenies of S strain was substantially greater, which also had significantly higher specific amylase activity than in F strain. The analysis of amylase activity variance showed that this variance among the males is 40 times, and among the females 2.7 times smaller in F than in S strain.
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Tetsuro Murota1995 年 70 巻 4 号 p. 497-504
発行日: 1995年
公開日: 2004/07/02
ジャーナル フリーIn a mouse specific-locus test in stem-cell spermatogonia of mice after treatment with N-ethyl-N-nitrosourea (ENU), a mutation at the hemoglobin α-chain locus was obtained. In an original mutant, the mutation was detected as a change in band density using cellulose acetate electrophoresis, e.g. the slow moving band was much higher than that of normal F1 mice. Yet, migration of the mouse bands was similar. In isoelectric focusing, the migration of a band toward cathode was observed, indicating that the variant hemoglobin is caused by mutation at an α-globin chain.
This mutation was found to be heritable and viable in homozygous condition from a genetic test on the mutant, suggesting that this mutation would be due to a base-pair change. According to the usual nomenclature of mutation at the hemoglobin locus, the allele symbol of this mutation was tentatively designated as Hbacc3.
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Takahisa Furukawa, Yoshitaka Kobayakawa, Kumiko Tamura, Ken-ichi Kimur ...1995 年 70 巻 4 号 p. 505-524
発行日: 1995年
公開日: 2004/07/02
ジャーナル フリーSuppressor of Hairless [Su(H)], the Drosophila homologue of RBP-Jκ is a novel type of sequence-specific DNA binding protein without known motifs, and highly conserved in various organisms. Su(H) regulates peripheral nervous system (PNS) development. Recently Su(H) was suggested to participate in the Notch-mediated signal transduction pathway. We show here that the Su(H) protein binds to TGTGGGAA sequence located 616 base-pairs upstream of the transcription initiation site of the Enhancer of split [E(spl)]m8 gene which is mapped to the terminus of the genetic cascade of the neurogenic genes. Su(H) transactivates the E(spl)m8 promoter not only in cultured Drosophila cells but also in vivo. The present study bridges the biochemical gap between Notch and E(spl) in the neurogenic gene cascade including Delta, Notch, deltex, Su(H), Hairless and E(spl).
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Ping Liang, Kazuhiro Kitada, Mitsuhiko Osaka, Tadao Serikawa, Rei Taka ...1995 年 70 巻 4 号 p. 525-531
発行日: 1995年
公開日: 2004/07/02
ジャーナル フリーErythropoietin stimulates proliferation and differentiation of erythroid progenitor cells by binding to a specific membrane receptor, erythropoietin receptor. By using the genomic clone derived from a rat cosmid library, the rat erythropoietin receptor gene was assigned to chromosome 8q24 by fluorescence in situ hybridization.
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Shoji Sonoda, Tetsuji Yamada, Tikahiko Naito, Fusao Nakasuji1995 年 70 巻 4 号 p. 533-542
発行日: 1995年
公開日: 2004/07/02
ジャーナル フリーDistribution of a family of tandemly repetitive sequences in Hemitaxonus japonicus (pYS family) was investigated among eight sawfly species and host races in the genus Hemitaxonus. High copy numbers of the repetitive sequences were detected in the two host plant races of H. japonicus, race Polystichum polyblepharum (race PP) and race P. tripteron (race PT), and H. sasayamensis, whereas a low copy number in H. minomensis. Comparison of the nucleotide sequences of the basic repeat units demonstrated a high degree of homology among the four tested species with high AT-rich sequences. Minor repeat units shorter than the consensus basic repeat unit of pYS family were found only in races PP and PT of H. japonicus. Analyses of their nucleotide sequences showed the occurrence of the host race specific sub-repeat units.
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Satoru Todokoro, Ryohei Terauchi, Shoichi Kawano1995 年 70 巻 4 号 p. 543-554
発行日: 1995年
公開日: 2004/07/02
ジャーナル フリーMicrosatellite polymorphisms of Arabidopsis thaliana were surveyed for 29 loci using 12 Japanese natural populations as material. All the individuals examined were homozygous at all the microsatellite loci. Although there was considerable variation among populations, no intra-population polymorphism was detected in three selected populations when eight loci were studied. The microsatellites examined proved to be a suitable genetic marker for studying the genetic variation among natural populations of Arabidopsis thaliana. To compare the genetic similarity and infer the evolutionary relationships among populations, neighbor-joining trees were constructed on the basis of distance matrices calculated by two different methods. Genetic relationships among the populations as revealed in the trees do not necessarily correspond to their geographic relationships. This may reflect their different origins or different times of introduction into the Islands of Japan.
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Kuo-Hai Tsai1995 年 70 巻 4 号 p. 555-562
発行日: 1995年
公開日: 2004/07/02
ジャーナル フリーRice cultivars so far examined mostly had the early-heading gene, Ef-1. Their wild progenitor, Oryza rufipogon Griff., was examined in respect to the genes for heading time in the present study. Of three strains tested, W593 from Malaysia and W1623 from Tauyuan, Taiwan produced no panicles when planted in the first crop season at Taichung, suggesting their strong sensitivity to photoperiod, while W630 from Burma was practically insensitive to photoperiod and produced panicles. In the second crop season, the wild strains headed several days later than Taichung 65. When they were crossed with Taichung 65, the heading dates of the F1 plants were similar to, or a few days earlier than that of Taichung 65. In the B1F1 (T65 × F1) populations, early-heading plants like Ef-1 carriers were found. They were backcrossed to Taichung 65 several times and true-breeding early-heading lines were obtained. When they were crossed with T65(20)E a carrying Ef-1a, the heading dates of the F2 plants varied within the parental range, suggesting that the wild strains had similar alleles at the Ef-1 locus.
Regarding genes controlling photoperiod sensitivity W593 and W1623 were found to have recessive ones denominated as se-7 (t) and se-6 (t), respectively. The effect of these genes can be suppressed by Ef-1 when combined together.
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