巻号一覧
20 巻, 6 号
選択された号の論文の6件中1～6を表示しています
- |<
- <
- 1
- >
- >|
-
第1報: 脳内MonoamineoxidaseおよびAcetylcholinesterase活性の季節変動小島 哲爾1966 年 20 巻 6 号 p. 351-358
発行日: 1966/02/10
公開日: 2009/02/17
ジャーナル フリーMany studies have demonstrated that the various changes occured in peripheral effectors of autonomic and endocrine systems under the affection of several environmental conditions. In this point, the auther intended to investigate the changes of monoamineoxidase (MAO) and acetylcholinesterase (Ach-E) activities in central nervous system which had a part of feedback control mechanism with peripheral effectors, and clarified the seasonal variations of MAO and Ach-E activities in rabbit brains. The experiments were carried out using 18 adult male albino rabbits bred under natural climate at Tsu-city (latitude 34°32′ N., longitude 136°31′ E.). The daily dry bulb temperature in this city was 12.5°C in spring, 27.3°C in summer, 17.6°C in autum, and 4.6°C in winter.
The animals were killed by air infusion into auricular vein, then the brain was removed and minced in seven parts; telencephalon, diencephalon, colliculus rostaris, mesencephalon, pons, myelencephalon and cerebellum. Each of the brain parts was homogenized with phosphate buffer by all-grass homogenizer.
The MAO activity was determined by Creasey and Davision's method; tyramin oxidation was calculated manometoricaly from the oxygen uptake during first 30 minutes of the reaction, and the Ach-E activity was also determined by Ammon's method; acetylcholine hydolysis was calculated from the volume of the carbon dioxide during first 30 minutes of the reaction.
The obtained results were as follows:
1. Both of these enzyme activities varied similary in the form of monophasic seasonal variation, except the Ach-E activity in colliculus rostaris which varied in the form of diphasic variation.
2. The reciprocal proportion of these enzyme activities was not observed on the distribution in the brain perts. But the reciprocal chenges of these enzyme activities were observed on the seasonal variation in telencephalon and diencephalon.
3. In winter, the decrease of MAO activity in telencephalon, pons, myelencephalon and cerebellum, and the increase of Ach-E activity in telencephalon, diencephalon, colliculus rostaris, mesencephalon and pons, were observed significantly.
4. Against winter, the antagonistic balance of these enzyme activities was observed in autumn especially in telencephalon.抄録全体を表示PDF形式でダウンロード (1019K) -
CPT動揺値とTAFの変動高桑 栄松, 池田 弘, 中平 成也, 土門 洋哉, 増川 寿治1966 年 20 巻 6 号 p. 359-363
発行日: 1966/02/10
公開日: 2009/02/17
ジャーナル フリーOur previous studies (part 5) on autonomic nervous balance and TAF revealed that the changes in TAF-L were parallel to the changes in autonomic nervous function tests such as the mecholyl test, cholinesterase activity and Kestner's reaction. This relationship was reexamined by the present experiment in which 21 examinees were exposed to a disagreeable noise and tested with the TAF test, mecholyl test and the cold pressure test (CPT). Swing degree in CPT was computed by the same procedure as that in mecholyl test, though the initial reactions are quite opposite: the rise of the blood pressure in CPT in contrast to the decrease of it in mecholyl test.
The results of this study indicated that:
(1) Exposure to noise brought about significant changes between the pre- and the post-exposure values in each of TAF-L, mecholyl test-swing degree and CPT-swing degree.
(2) The significant correlation between TAF-L and the swing degree in mecholyl test has been reconfirmed.
(3) Swing degree in CPT showed a significant increase from 1.2 for the pre-exposure to 1.5 for the post exposure average. The changes significantly correlated with TAF-L.
(4) Swing degree in CPT also correlated significantly with that in mecholyl test.
The relationship between TAF, mecholyl test and CPT in individual patterns are shown in the figure. Swing degree in mecholyl test has been applied to CPT for the first time by our present study. These observations suggested that the decrease in TAF-L under the exposure to noise has a certain correlation with the increase of homeostatic defence in the hypothalamus, and that the swing degree in CPT, which is less reactive on the examinee than the mecholyl test, can positively be used for testing the autonomic nervous function with the same significance as the mecholyl test.抄録全体を表示PDF形式でダウンロード (648K) -
庄司 光, 山本 剛夫, 西田 耕之助, 尾崎 良嗣1966 年 20 巻 6 号 p. 364-376
発行日: 1966/02/10
公開日: 2009/02/17
ジャーナル フリーBy using high sensitive gas chromatogrph (equiped with flame ionization detector, sensitivity: 2mV/200mm full scale) 46 commercial laquer thinners and 10 organic solvents used in three industries (in Kyoto City) were analyzed.
Tricresylphosphate (supported on firebrick C-22, 80-100 mesh) was used as stationary phase and maintained at 100°C. Column sizes were 4mm I.D. and 4m L. The purified nitrogen was used as the carrier gas and its flow rate was regulated at 38-40ml/min. Each sample solvent of 4-8μl was injected into the gaschromatographic system by microsyringe.
Each peak appeared on the chromatogram was identified by comparing with the relative retention volumes (VR) for the standard substances (VR of benzene=1.000). The quantitative determination of component was performed by applying the peak area method (half band width method).
The results were as follows:
1. On each chromatogram, 12-43 organic components were identified.
2. The average concentration of the contents classified by chemical structures were as follows: aromatics 36%, aliphatics 11%, chlorides and alcohols 10%, acetates 7%, ketone 1%, and aldehyde 0.3% respectively.
3. The average concentrations of important components which could be detected were as follows: toluene (17%), total xylene (m.p.o.) (12%), benzene (7%), trichloroethylene (6%), butylalcohol (n.i. sec. tert.) (5%), tetrachroroethylene (4%), methylmetacrylate (3%).
4. Among the detected components in sample solvents, the concentration of benzene was the highest (48%).抄録全体を表示PDF形式でダウンロード (1243K) -
鈴木 継美, 竹本 泰一郎, 深山 智代1966 年 20 巻 6 号 p. 377-382
発行日: 1966/02/10
公開日: 2009/02/17
ジャーナル フリーTwo experiments were performed to clarify whether or not the content of casein in the diet changes the susceptibility to benzene in mice. As the indicators for anlysis, the number of leucocyte, hemoglobin content, weights of the thymus, spleen, liver and kidney, and body weight were determined after the repeated subcutaneous injection of benzene with varied doses. Low content of casein in the diet (5%) was proved to decrease the body weight, and the weights of some organs and the administration of benzene decreased the number of leucocyte and weights of the thymus and spleen. Though the influences of benzene administration and of content of casein were certainly addititive on some indicators, such as the weights of thymus and spleen, no significant synergistic influence was observed in all the indicators. Conclusively, it was assumed that there might be the least possibility in increasing of the susceptibility to benzene by the low content of casein in the diet.抄録全体を表示PDF形式でダウンロード (598K) -
相磯 和嘉, 蟹沢 成好, 岡本 達也, 沖田 正彦, 中条 朋子1966 年 20 巻 6 号 p. 383-397
発行日: 1966/02/10
公開日: 2009/02/17
ジャーナル フリーTwenty month feeding studies with tylosin using Wister strain rats were carried out to estimate the toxicity and the safety of this antibiotics as a food additive. A total of 160 albino rats, weighing 80∼115g was used. Five groups of 16 each, equally divided as to sex, were fed diets containing 0.1, 0.01, 0.1, 1.0 and 10.0% of tylosin, repectively, for a 20-month period. The results obtained were following. Macroscopically, during the course of experiments, no difinite signes of toxic effects for the animals were observed in each drug levels; such as food cosumption, appearance of skin, hair and state of motility.
From an analysis of growth curves, no significant differences were shown between the groups fed tylosin and control in body weight gain, even at the high level of 10% drug diet. After a feeding period of 20-month, 75, 43, 31 and 30% of the rats survived at the drug level of 10, 1.0, 0.1, 0.01% respectively, while only 26% of the control animals survived. Postmortem examination in experimental animals did not show any pathological changes in liver, kidney and other main organs. Haematological testes were also shown normal limits.
Microsckopic studies on the main organs of the rats sacrificed at 12-month and 20-month were made in detail. Degenrative changes, atorophy, hydropic degeneration, pyknosis and fatty metamorphosis of hepatic cell were not demonstrated in all cases. Any cirrhotic changes in liver were not observed. There was nothing of the neoplastic changes in hepatic cells of all the rats tested. Remarkable toxic effect in the kidney, spleen, heart and testes was also not observed.抄録全体を表示PDF形式でダウンロード (7825K) -
特にカルシウム欠乏との関係について石崎 有信, 田辺 釧, 松田 悟, 坂元 倫子1966 年 20 巻 6 号 p. 398-404
発行日: 1966/02/10
公開日: 2009/02/17
ジャーナル フリーSome albino rats were fed with diet highly defficient in calcium and insufficient in protein. A certain quantity of cadmium dissolved in drinking water was given. After half a year they were sacrified to be examined pathologically.
The most significant microscopical finding in kidney was the degeneration of tubules. In most cases the decalcification of bone was apparent, especially in female rats, some of which might have contracted osteomalacia.
These findings may give a clue to the pathogenesis of so-called “Itai itai” disease.抄録全体を表示PDF形式でダウンロード (919K)
- |<
- <
- 1
- >
- >|