Acute and chronic toxicity were investigated experimentally for new Nitrofuran derivatives-Panfuran, Panfuran-acetate. Panfuran-diacetate and Paufuran-base. The intra-peritional lethal dose of 50 percent (LD 50) of mice was found to be 0.13gm/kg. of body weight in Panfuran, 0.435gm/kg. in Panfuran-acetate and 0.147gm/kg. in Panfuran-diacetate respectively. In subcutaneous administration, the LD 50 could be found to be 0.597gm/kg only in Panfuran. The oral LD 50 of rats was found to be 3.22gm/kg. in Panfuran. The chronic toxicity of these compounds was studied for one year using King Wister strain rats for feeding experiments. Five males and five females were placed in each of the diet groups, 1/10, 1/500 and 1/1000 of oral LD 50 of them.
The results obtaind in the feeding experiments were as follows:
1. According to an analysis of group curves, reduction of the natural gain of body weight was observed on the rats which were fed with the diet incorporated, with only 1/10 dose of oral LD 50 in each of the four drugs. This reduction was equally observed in both sexes and it was more severe in the female than in male.
2. As for histological findings, main changes were observed in liver, kidny. spleen and testis. Necrobiosis, degenerative changes, pyknosis and fatty invasion of hepatic cell and confusion of liver strings were observed in all groups of rats. These were the most severe in the case of 1/10 dose of Panfuran. All other diet groups except those not demonstrating necrobiosis, but, other pathologic findings of hepatic cells were slightly revealed in these cases.
3. Degenerative changes and cloudy swelling of epithelial cells of renal tubules and renal cylinders were observed in all diet groups of four drugs. However, hepatic changes were rather mild in Panfuran groups, and especially in Panfuran-base groups, they were markedly observed without suggesting any difference of drug intake.
4. Pathological changes for the testis were characteristically demonstrated. Marked atrophy of seminifeous epithelium were identically caused by Panfuran; Mature spermatozoid and spermatid were rarely discovered and also conspicuous reduction of spermyocyte was observed. These pathological findings were even presented in Panfuran-acetate groups but atrophy of testis was only observed in one group of 1/10 dose of LD 50 which was not so severe as Panfuran groups.
5. From these results, it is suggested that the toxic effects of Panfuran-acetate and Panfuran-diacetate are no less mild than that of both Nitrofurazone and Nitrofurylacrylamide allowing actual food additives. The use of the former two Nitrfuran derivatives is usually available for food preservatives.

抄録全体を表示

The purpose of this study was to reveal the physiological effects on rabbits, and inhalated air containig small amounts of CO. This was especially noted on the changes of the percent of saturation of carboxy hemaglobin and blood sugar levels.
The flexible film isolator for rearing germ free amimals was applied to make a chamber to flow constantly the desired concentration of CO throughout these experiments.
The experimental results were summarized as follows:
1) The peak of percentage of saturation of carboxyhemoglobin was observed after inhalation of CO in the concentration of 100ppm. for about 30 to 60 minutes, and its value was decreased gradually in half after 3 hours in spite of the same situation under which the animals breathed. In spite of administration of Epinephrine in order to check the influence with an increased circulatory volume, the value was not decreased under the same CO conditions.
2) The inhalation of CO in the concentration of 100ppm. caused the increase of blood sugar levels in rabbits and the peak of the levels was observed after the inhalation for the mean period of 90 minutes. The continuation of hyperglycemia appeared even after discontinuation of inhalation of CO of 300ppm.

抄録全体を表示

An investigation was made on noise and air pollution caused by automobiles passing Kanmon Tunnel from 9:30a.m. to 4:30p.m. on Feb. 26, 1964, The positon taken was at the centre of the tunnel. This tunnel is 3461.4m. in length and is one of the most important links between Honshu and Kyushu Islands of Japan. The investigations took place every 30 minutes and the following is the result.
1) Three thausands two hundreds and eighty one autmobiles proceeded through the tunnel's entrance over 7 hour period. The expected increase is arise to a approximately 12, 000 per day by 1970.
2) Suspended dusts in the tunnel were sampled by the “Roken Type Dust Sampler” like Owen's Jet Dust-Sampling Instrument and were counted using a microscope as is usual. The mean amount of Suspended dusts was 824/ml. of the air. More than 1000/ml. of dust was detected twice out of six times in the a.m., and 3 out of 8 times in the p.m. Such a condition is the most infavorable for laborers in the area.
3) Concentrations of CO₂ were examined twice every 30 munites using “Kitagawa's Indicator Sampler.”. The concentration of CO₂ didn't reach a remarkable high level, but was between 0.05%-0.07%. The mean value of this test is 0.059%.
4) Carbon Monoxide, an important part of the study of air pollution caused by automobiles, was checked regularly close to the entrance of the tunnel. The concentration of CO which was measured location was between 0.01% to 0.03% and the mean value was 0.03%.
5) Sulfur dioxide in the air, which may be exhausted mainlyfr om diesel engines, was absorbed by the HgCl₂-NaCl solution in a small impinger bottle. Next the concentration of SO₂ was measured by colorimetry method using p-rosaniline and formaline as reagents. The mean concentration and the maximum concentration of this survey was 0.189 and 0.239p.p.m. respectively.
6) Sound level was checked 5 times every 30 minutes using a sound level meter. Maximum sound level from trucks was 116 phon (A). The level of the back ground noise in the tunnel was between 82 phon (A) to 100 phon. As a result of octave band analysis, the over all sound level had a very high intensity and the main frequency of noise in the tunnel was composed of low frequency. The results of this investigation above sound level, concentrations of carbon monoxide and sulfur dioxide in this particular tunnel were unexpectedly over the normal range levels. Therefore, it can be concluded that an increase of ventilation volume should be undertaken while an attempt bema de to decrease the concentration levels of exhaust gases given off by the vehicles under consideration.

抄録全体を表示

京都市小中学生計約80,000名の1966年4月の身長, 体重, 胸囲, 座高の測定値を資料として, これらの身体計測値間の相関, 偏相関並びに体重の他の身体計測値に対する重回帰を学年別男女別に求め, 現在の都市小中学生における身長, 体重, 胸囲, 座高の相互関係をあきらかにした。また, 身長と体重との相関並びにその年齢的推移を, 文部省が昭和35年に11歳, 14歳の全国の男女について求めたもの, 永井が約20年前に新潟市で12歳以上の学生生徒について求めたものと比較して, 現在の都市小中学生の体格の特微並びに成長発達の促進について言及した。
さらに, 身長, 体重, 胸囲, 座高の間の6つの相関からなる相関行列が学年男女間で異なっている場合の多いこと, なるべく簡潔に体格の特徴をあらわすには, 身長, 体重の二つの計測値を用いるのが適当であることを指摘し, また, 体重の他の身体計測値に対する重回帰からの偏差の原因について考察した。

抄録全体を表示