巻号一覧
26 巻, 6 号
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亜硫酸塩の微生物発育に及ぼす影響陳 瑞雲1972 年 26 巻 6 号 p. 467-474
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリーSulfite acted as a strong antibacterial agent both to Staphylococcus aureus and Bacillus subtilis. With 1mM sulfite in the growth medium, the lag phases of these two microbes were remarkably extended, especially in the lower pH range of growth.
Riboflavin was found to be effective for growth restoration of Staphylococcus aureus and Bacillus subtilis. With 1μg/ml to 10μg/ml of Riboflavin, these microbes were not only free from growth repression by sulfite but rather their growth was stimulated. The extent of growth restoration effect of Riboflavin depended on the concentration of sulfite and Riboflavin, pH and addition time of the growth medium. Neither sulfite or Riboflavin were effective for growth repression or restoration to the gram-negative bacteria tested. Growth inhibition of Candida albicans with sulfite was restored by adding thiamine to the culture medium.抄録全体を表示PDF形式でダウンロード (920K) -
亜硫酸塩の微生物の中間代謝に及ぼす影響陳 瑞雲, 坂口 平1972 年 26 巻 6 号 p. 475-480
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリーEffects of sulfite on glucose and amino acid metabolism were examined with the following results:
1. The inhibitory effect on glucose oxidation of intact cells was not clearly observed with 1mM sulfite in the reaction medium, but TCA cycle metabolites such as malate, pyruvate and succinate showed a fairly good inhibition with the same concentration of sulfite. Sulfite showed a greater inhibition to glutamate and alanine oxidation of intact cells of Staphylococcus aureus than that of Escherichia coli and Proteus vurgaris.
2. By pre-incubation with sulfite, lactic, succinic dehydrogenase and L-amino acid oxidase in cell free extracts showed considerable low activity.
Increased concentration of sulfite and prolongation of pre-incubation time resulted in enlargement of inhibitory activity. Aminotransferase of Escherichia coli was also inhibited with sulfite.抄録全体を表示PDF形式でダウンロード (821K) -
硫黄酸化物の微生物水解酵素ならびに消化酵素に及ぼす影響横田 勝司, 陳 瑞雲, 坂口 平1972 年 26 巻 6 号 p. 481-485
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリーEffects of four food additives, sulfite, bisulfite, thiosulfate and metabisulfite, on the protease, amylase, acid- and alkaline-phosphomonoesterase activity of Takadiastase A, Asp. fumigatus, NAGARSE (Bacillus subtilis), Pepsin, Trypsin, pancreatic amylase, salivary amylase were studied. Salivary amylase markedly inhibited hydrolysis of soluble starch with pre-incubation by sulfite, bisulfite and metabisulfite, respectively, but not by thiosulfate. Pancreatic amylase was also inhibited by the same sulphur oxides.
Degree of inhibition by sulphur oxide was less than that by salivary amylase.
Measurement of amylase activity in the presence of a reducing agent such as sulphur oxides was established. Pepsin was weakly inhibited hydrolysis of casein with pre-incubation by sulfite, bisulfite, thiosulfate and metabisulfite, respectively.
Trypsin was also inhibited by thiosulfate and metabisulfite. Microbial hydrolytic enzymes of Takadiastase A, Asp. fumigatus and NAGARSE were not effected by sulphur oxides.抄録全体を表示PDF形式でダウンロード (617K) -
アミラーゼ阻害, 特にだ液アミラーゼについて陳 瑞雲, 横田 勝司, 坂口 平1972 年 26 巻 6 号 p. 486-491
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリーSulfite inhibited α-amylase activity of saliva, Asp. fumigatus. hen serum and mouse serum. Addition of sulfite to the enzymatic reaction system had no effect on the amylase activity. Pre-incubation time was necessary for inhibition of salivary amylase with sulfite. The enzyme inhibited with sulfite was reactivated by addition of an activator such as NaCl and the enzyme activated with NaCl was inhibited by the addition of inhibitor such as sulfite. With the addition of hydroquinone inhibition of enzyme with sulfite stopped. Immunological reaction between anti-salivary amylase serum and sulfite-enzyme or native enzyme had no effect on the quantitative precipitin and agar diffusion. Administration of sulfite had no effect on blood amylase activity under several in vivo conditions.抄録全体を表示PDF形式でダウンロード (834K) -
亜硫酸塩の存在における硝酸, ニトロ還元酵素活性とメトヘモグロビン生成について坂口 平, 陳 瑞雲, 横田 勝司1972 年 26 巻 6 号 p. 492-497
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリーEffects of sulfite on nitrate reductase, nitroreductase activities and methaemoglobin (Met. Hb) formation were reported with the following results. Nitrate reduction by washed cells of Escherichia coli was accelerated by the presence of sulfite in the reaction medium. On the other hand, reduction of O-nitrophenol was diminished by the concentration of 10-1∼10-2M of sulfite whereas it was accelerated at 10-3∼10-5M. Similar results on the reduction in bouillon culture medium were also observed. Met. Hb formation of rabbit erythrocyte suspension with nitrite showed a decrease with the addition of sulfite as compared with the control containing no sulfite. It was also found that levels of Met. Hb in erythrocyte contacted with nitrite revealed a remarkable tendency to decrease after erythrocyte was pre-incubated with sulfite.抄録全体を表示PDF形式でダウンロード (762K) -
(1) 重金属, ビタミン類前投与がマウスの急性カドミウム中毒に及ぼす影響阿部 登茂子, 糸川 嘉則, 井上 喜久子1972 年 26 巻 6 号 p. 498-504
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリーEffect of pre-administration of heavy metals and vitamins on acute cadmium poisoning were clarified in experiments done on mice.
In group 1: large doses of cadmium (3mg/kg. body wt.) resulted in testicular atrophy and congestion. Splenic hypertrophy, kidney atrophy and haematocrit decrease was also seen. Necrosis in testicular tubules was histochemically observed. Biochemical results were: Zn and Mg contents decreased in testis, Zn contents increased in liver and kidneys plus an increase of cadmium in various tissues.
In group 2: small doses prior to large doses of cadmium prevented the abnormalities seen in group 1 except that cadmium concentration in testis and liver was increased to a greater extent than in a single administration of large doses of cadmium.
Mn, Zn, ascorbic acid, thiamine or thiamine propyl disulfide were administered prior to cadmium. Although prevention of a decreased haematocrit resulted, testicular atrophy and necrosis were still observed.
Tentative explanations for these mechanisms have been presented.抄録全体を表示PDF形式でダウンロード (1317K) -
第1報 在日米軍と自衛隊員の基礎代謝季節変動の比較吉田 啓峻1972 年 26 巻 6 号 p. 505-512
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリーDuring the period between June 1969 and May 1970 basal metabolic rate was experimentally conducted on 10 Japan Maritime Self-Defence Force members and 9 U.S. Marine Corps members both stationed at Sasebo. Results are as follows:
Under the West-Japan climate, it was confirmed that seasonal variation of BMR was noted on Caucasians as well as Japanese. The pattern of the variation showed a two-peaked curve which lowered in mid-summer and mid-winter in case of Americans against the variation of a one-peaked curve annually among Japanese. Lowered metabolism in Americans in the winter was thought due to reduction of work loads and less outdoor exposures. The fact that they live a more comfortable temperature that is about 10 degrees higher than temperatures in usual Japanese living conditions should be considered. As for RQ, that of Americans was always lower than Japanese throughout the year. These probably can be attributed to differences in daily dietary fats.抄録全体を表示PDF形式でダウンロード (1034K) -
第2報 日本気候下の日米両国人血清蛋白結合沃度値の季節変動桑野 紘一, 山口 洋一, 吉田 啓峻, 平田 文夫, 中村 正, 菅原 和夫, 槌本 六良1972 年 26 巻 6 号 p. 513-517
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリーAs a series of studies on the seasonal variation of physiological functions, protein-bound iodine in serum was determined along with the BMR. An experimental period of one year commencing in June 1969 included 10 Japanese and 9 Americans the same as stated in the first report. Conclusions are as follows:
1) As for the annual mean value of serum PBI, there was no significant difference between Japanese and Americans, however, both groups showed significant seasonal variations to be low in summer and high in winter.
2) As for Japanese, serum PBI level was higher in the cold winter, but it was noted to be at a high level in Americans during the cool autumn season rather than cold winter season. This is attributed to utilized warmer room temperatures than that of Japanese, as mentioned in the first report on BMR.
3) Positive correlation between PBI and BMR on both groups was found to be significant.
4) Regarding relationship to the mean external temperature, PBI showed higher values in autumn than in spring under the same external temperatures. Furthermore it was found that the rate of PBI varied earlier and higher than that of BMR according to seasonal changes.抄録全体を表示PDF形式でダウンロード (610K) -
第3報 日本の気候下の日米両国人血清遊離脂肪酸の季節変動山口 洋一1972 年 26 巻 6 号 p. 518-525
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリーIn addition to the previous two reports submitted regarding the BMR and PBI in serum on the Japanese and American nationals, this experiment was conducted on the free fatty acids in serum of the same subjects with those in the first report, for about one year commencing in June 1969.
Results are as follows:
1) As for serum FFA, results were higher in Americans than in Japanese.
2) As for relationship between serum FFA and the RQ in basal metabolism a significant inverse correlationship was noted in subjects with a higher serum FFA showed a higher fat combustion rate, however, this relationship was significant only for Japanese.
3) Relationship between the serum FFA and BMR showed a seasonally slight different trend. While FFA and BMR correlated positively in summer, the relation inclined to be reverse during the period of cold adaptation.
4) As for relationship between serum FFA and external temperature, FFA showed a high value during the season in which the temperature was higher. It was noted that there was a tendency for the FFA level to lower during low temperature season. This trend was clearly noted on Japanese nationals, however, almost no such tendency was observed in American nationals.
5) A significant correlationship of FFA and PBI was not noted in this experiment.抄録全体を表示PDF形式でダウンロード (1020K) -
第2編 アルキル化合物の生体内動態の研究早川 清子1972 年 26 巻 6 号 p. 526-535
発行日: 1972/02/28
公開日: 2009/02/17
ジャーナル フリー第1編で報告した分析法を用いて, 3ML, 3EL, 4ML, 4ELを投与したラッテ, マウスにおける臓器中の4AL, 3ALの生体内動態を検討した。
4ML投与ではラッテの3ML臓器分布はマウスに比べて著しく低く, 4ELではラッテ, マウスではその差はあまり大きくなかった。ラッテでは臓器からの3ALの減少がマウスに比べておそい。これは4ALが一たん3ALになって臓器にたくわえられるとそれはなかなか減少しがたいことを示した。
またマウスに4ML, 4ELを投与した場合, 体内においてこれらが3ML, 3ELへ分解する速度の割合を検討した。4MLは2日後に体内の3ML量が最高に達して投与量のほぼ50%が3MLに変化した。一方4ELは3日後に約30%が3ELに変化した。この場合, 投与溶媒により体内への吸収速度が異なるので体内量が最高に達する時間も異なる。4MLはマウスに毒性が強いのに対して, ラッテは弱く, 4ELはマウスとラッテであまり差がみとめられなかったが, わずかにラッテに強い傾向を示した。
加鉛ガソリン使用作業者はその接触度に応じて尿中のアルキル鉛が検出される。このことから尿中のアルキル鉛の測定を行なうことにより4ALによる暴露度を知り, 健康管理の一指標とすることができる。抄録全体を表示PDF形式でダウンロード (1053K)
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