巻号一覧
30 巻, 6 号
選択された号の論文の7件中1～7を表示しています
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(V) Methiin の Methionine 代用性とアミノ酸 imbalance の有無について八木 典子, 高島 真知子, 糸川 嘉則1976 年 30 巻 6 号 p. 593-599
発行日: 1976/02/28
公開日: 2009/02/17
ジャーナル フリーComparative studies on the effects of S-methylcysteine sulfoxide (SMCS), S-allylcysteine sulfoxide (SACS) and methionine on rats were carried out.
Experiment No. 1
Young male Wistar rats were separated into five groups and five synthetic diets (amino acids mixture was used as protein source) were given. To group 1, methionine and cystine deficient diet; to group 2, SMCS supplemented to methionine and cystine deficient diet; to group 3, SACS supplemented to methionine and cystine deficient diet; to group 4, cystine supplemented to methionine deficient diet; and to group 5, complete amino acid mixture diet were given.
In group 5, good growth was observed while body weight in group 1, 2, 3 and 4 showed progressive loss and after 20∼30 days these animals died.
These facts suggested that SMCS, SACS and cystine had no methionine-substitution effect on the growth of methionine-cystine deficient rats.
Experiment No. 2
Adult male Wistar rats were separated into six groups and six semisynthetic diets were given. Group 1, low protein diet (5% casein); group 2, high protein (15% casein); group 3, low protein plus 2% methionine; group 4, high protein plus 2% methionine; group 5, low protein plus 2% SMCS; group 6, high protein plus 2% SMCS containing diet. After two weeks of these dietary regimens, the animals were sacrificed and various examinations were carried out.
Increase of liver triglyceride, abnormal pattern of fatty acids in liver and decrease in serum threonine were prominent in group 3. While in the other groups such abnormalities were not seen.
From these findings, it was clarified that even when an excessive amount of SMCS was added to the low protein diet, this amino acid do not produce symptoms of amino acid imbalance as seen in rats fed excessive amount of methionine.抄録全体を表示PDF形式でダウンロード (3996K) -
大気中Cd, Pb, Fe, Mnのラット体内蓄積に関する研究石見 敦子1976 年 30 巻 6 号 p. 600-614
発行日: 1976/02/28
公開日: 2009/02/17
ジャーナル フリーAmong the air pollutants, oxides of sulfer and nitrogen as well as oxidant produce acute symptoms on the mucosa and others, while many of metal elements do not. But the latter, once introduced into the living body remain there longer than the former. The author has paid attention to a serious fact that air-polluting metal elements are unconsciously accumulated in the body to exert chronic effect, and so the animals bred in a general living environment to observe the effect of corpuscular substances which are contained in air pollution.
As experimental animals, rats were used. They were bred at different places in Tokyo-in Ohta-Ku, where general metal pollution is at higher grade, and Koganei city where it is low, and the animal laboratory of this university as control. The breeding started at the age of 10 weeks under the same conditions but in different places. At 4, 8 and 12 months of breeding, metals (Cd, Pb, Fe, Mn) in the lungs, brain, liver, kindneys, blood and feces from the rats of the three places were assayed, and their lungs, livers, kidneys and spleens were microscopically observed, with the following results;
1) The lung content of each metal was significantly higher in Ohta-Ku than in Koganei city at all of the 4, 8 and 12 months. As for the other organs, Cd content in the brain and kidney at 4 months, Pb in the brain at 4 months, in blood at 4 and 12 months, and in feces at 4 and 8 months, Fe in the liver at 4, 8 and 12 months, in blood at 8 and 12 months and feces at 4 months, and Mn in the liver at 12 months were significantly higher in the breeding place of Ohta-Ku than Koganei city.
2) Gross observation of the lung on sacrifice revealed marked deposition of coal powder-like substance in the Ohta-Ku group, but not in the Koganei city group.
3) Microscopically, phagocytes, ingesting dust were observed in the subpleura, pulmonary alveola, bronchus, perivascular region as well as in lymph nodes, lymph follicles and their surroundings.抄録全体を表示PDF形式でダウンロード (4826K) -
山口 正義, 佐藤 秀司, 山本 丈夫1976 年 30 巻 6 号 p. 615-618
発行日: 1976/02/28
公開日: 2009/02/17
ジャーナル フリーThe effect of lead acetate (Pb; 25.3μmole/100g body weight) on the kidney calcium accumulation, the decreased serum calcium concentration, and the increased serum inorganic phosphorus concentration induced by the administration of stannous chloride (Sn; 25.3μmole/100g) was studied. The predominent kidney calcium accumulation, the decreased serum calcium and the increased serum inorganic phosphorus concentrations produced at 72 hours after a single intraperitoneal administration of stannous chloride was significantly inhibited when lead acetate was administered 24 hours before the administration of stannous chloride or at the same time. On the other hand, when lead acetate was administered 24 houres after the administration of stannous chloride, lead had no effect on revelation of calcium metabolic disturbance in rats treated with stannous chloride. The data obtained from the present experiments suggest that the lead acted antagonistically on the accumulation of tin into the kidney cells and inhibited the manifestation of the renal effect by tin.抄録全体を表示PDF形式でダウンロード (589K) -
千葉 百子, 田代 明子, 菊池 正一1976 年 30 巻 6 号 p. 619-626
発行日: 1976/02/28
公開日: 2009/02/17
ジャーナル フリーThe effect of the heat treatment, which was applied under various conditions, on δ-aminolevulinic acid dehydrase (ALAD) activities was investigated by means of determination of ALAD activities in the peripheral blood of normal rabbits (N-rabbit) and intravenously lead-injected rabbits (Pb-rabbit). Furthermore, lead was added in vitro to N- or Pb-rabbits' blood, then ALAD activities were assayed with and without heat treatment. The following findings were obtained:
(1) ALAD activity of the N-rabbit blood decreased monotonously with increase of temperature and time of the heat treatment. (2) Decrease of ALAD activity of the Pb-rabbit blood is shown in Table 1. (3) In both N- and Pb-rabbits' blood, the ALAD activities after heat treatment at 60°C for 5min converge to a definite value, which is about 1/3 of ALAD activity of the N-rabbit blood, the value being nearly constant and independent of the doses of lead administration. It was revealed that an increase of the activity by the heat treatment in the Pb-rabbit blood takes place only in cases in which the initial activity is lower than this value. (4) When lead was added to the N- or Pb-rabbit blood in vitro, (a) ALAD activity was not affected by 2.9×10-10M Pb concentration in incubation mixture in both cases. (b) By 2.9×10-8M Pb ALAD activity fell to about 50% in the N-rabbit blood and to 40∼20% in the Pb-rabbit blood, the latter depending upon the doses of lead previously administered in vivo. (c) Decrease of ALAD activity by heat treatment was enhanced by adding lead in vitro, and this effect was more remarkable in the rabbit blood which was affected by previous administration of lead. (5) Preincubation of the N- or Pb-rabbit blood with lead only enhanced the decrease of ALAD activity, without showing any increase in the activity.抄録全体を表示PDF形式でダウンロード (1137K) -
吉本 佐雅子, 浅野 恭代, 勝野 真吾, 阪本 州弘1976 年 30 巻 6 号 p. 627-631
発行日: 1976/02/28
公開日: 2009/02/17
ジャーナル フリーAn analysis of aggregation month of perinatal death for each prefecture was down in 1971 with the following results.
1) The late foetal death by prefecture shows a marked trend to aggregate during Junuary to April, and the early neonatal deaths mainly aggregate from July to October for east Japan, but it shows a tendency to aggregate from February to April in west Japan. So it seems that the cold weather in winter becomes a cause of the perinatal deaths especially in the late foetal deaths, and the hot weather in summer mainly influences to the early neonatal deaths.
2) The early neonatal death rate seems to increase corresponding to the high rate of the birth in home for each prefecture, but this tendency dose not clarify for the late foetal deaths.
3) Eighty-five percent of the early neonatal deaths is considered to be the higher death rate group which is almost dead within 3 days after birth by the force of mortality of 1.11, and the other fifteen percent shows the moderate death rate having the force of mortality of 0.17. So, it seems that the mortality rate within 3 days after birth may become an index for neonatal deaths.抄録全体を表示PDF形式でダウンロード (542K) -
第2報 単分散球形金属ヒュームのラット肺内への沈着率本間 克典1976 年 30 巻 6 号 p. 632-638
発行日: 1976/02/28
公開日: 2009/02/17
ジャーナル フリーFrom the results of theoretical and experimental studies on the aerosol retention in lung, it has been assumed that the effect of particle size on the lung retention rate of aerosol would uniformly depend upon the aerodynamic mass median diameter (AMD or Da).
In order to elucidate this assumption, the author made an inhalation experiment using lead (Pb) and tellurium (Te) fumes which had monodispersed spheric but different specific gravities for rats. In the experiment several particle sizes of Pb or Te fume generated by the high frequency electric furnace type fume generator were inhaled by SPF male rats of Sprague-Dawley strain in a head-only exposure chamber for 90min. After inhalation, the lung was taken out instantly from the rat's body, and the weights of metallic fumes retained in each rat lung were measured by atomic absorption analysis. The relative lung retention rates (RL) of metallic fumes in rats were calculated considering the exprimental conditions such as the fume concentration in the exposure chamber, the exposure period and respirating minute volume of rat. However, minute volume of each rat could not be measured easily, so that it was used uniformly 100ml/min for minute volume of rats because body weight of each rat showed little difference among each other.
As results of the experiment, the following relationship was found to exist between RL and Da in both the cases of inhalation of Pb and Te fumes: RL=K⋅Da-n. The author named the index number of lung retention as n in this equation. According to the above assumption, n value should be constant regardless of the kind of aerosol material. However, n were 0.72 of Pb fume and 0.36 of Te fume in the present experiment. This difference might be produced by the physico-chemical properties and biological activity of metallic fumes.抄録全体を表示PDF形式でダウンロード (4088K) -
第3報 多分散不整形ヒュームのラット肺内への沈着率本間 克典1976 年 30 巻 6 号 p. 639-644
発行日: 1976/02/28
公開日: 2009/02/17
ジャーナル フリーSeveral kinds of polydispersed irregular metallic fumes, which were generated from antimony, cadmium and lead chloride by the high frequency electric furnace type fume generator, were inhaled by SPF male rats of Sprague-Dawley strain in a head-only exposure chamber for 90min in order to study the effect of particle size, biological activity and solubility of these fumes on the lung retention rate in rats.
Results obtained from the inhalation experiment were as follows.
1) Effect of particle size on the relative lung retention rate (RL) was the same as that obtained from the inhalation of monodispersed spheric metallic fume. Furthermore, the following relationship was found to exsit between RL and aerodynamic mass median diameter (AMD or Da): RL=K⋅Da-n.
2) The index number of lung retention (n) of 1.34 in antimony fume was larger than that in lead or tellurium fume. This means that dependency on particle size was greatest in lung retention of antimony fume.
3) RL of cadmium having irritant effect on lung was of about the same value as that of lead fume which was inert.
4) RL of lead chloride which dissolve to an enormous volume in water was of the same value as that of lead fume which dose not dissolve in water.
As a result of a series of metallic fume inhalation experiments, the author could recognize that the lung retention rate of metallic fume not only depended upon particle size but also influenced by the biological activity of each metallic fume.抄録全体を表示PDF形式でダウンロード (3654K)
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