The following disulfide compounds: 2-hydroxyethyldisulfide (2-ME
OX), 2-aminoethyldisulfide (cystamine) and oxidized dithiothreitol (DTT
OX) were found to augment the in vitro antibody response to sheep erythrocytes in murine lymphocytes as effectively as their reduced forms when they were added to the culture medium. We, however, found out that the mode of action of DTT
OX was greatly different from that of 2-ME
OX or cystamine. 2-ME
OX and cystamine showed augmenting effects on the proliferative response to lipopolysaccharide and on the uptake of (
35S)-cystine by the lymphocytes. In contrast to these intermolecular disulfides, DTT
OX, an intramolecular disulfide compound, was found to be inactive in these systems. 2-ME
OX and cystamine, but not DTT
OX, were reduced to thiol forms by the intact lymphocytes or by the cell homogenate. Thus, it is likely that DTT
OX did not behave as the reduced form in the lymphocyte culture in contrast to 2-ME
OX and cystamine, suggesting that the disulfide form itself plays an important role in augmenting effects of DTT
OX.
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