In the present study, we investigated the effect of adenosine triphosphate(ATP)on cytosolic free calcium mobilization and mitogenic activity in cultured bovine corneal endothelial cells(BCEC).The[Ca
2+]
i was determined using a Ca
2+ sensitive indicator, Fura-2/AM, and cell proliferation was evaluated by counting the cell number.ATP, its metabolites and analogs caused transient increase in[Ca
2+]
i in a concentration-dependent manner(10
-7M−10
-3M)and the potency of agonists was ordered as follows:2-methylthio-ATP>uridine triphosphate>ATP>adenosine diphosphate.Adenosine monophosphate and adenosine did not affect[Ca
2+]
i.ATP(10
-4M)also promoted the accumulation of inositol trisphosphate(IP
3).The ATP-induced transient[Ca
2+]
i increase and IP
3 accumulation were attenuated by pretreatment with a phospholipase C inhibitor, U-73122(5μM), for 30 min.ATP(10
-5M)significantly enhanced the proliferation of BCEC.ATP-induced[Ca
2+]
i increase and cell proliferation were inhibited by a purinoceptor antagonist, suramin(10
-4M).Thus, the present study indicates that BCEC contain P2 purinoceptors that regulate their proliferation.
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