Bactericidal effect of chlorhexidine (CH) on clinically isolated bacterial strains was examined. Twenty-one isolates out of 61 (40 from inpatients samples and 21 from hospital environment) survived after the treatment of 0.5% chlorhexidine for 10 sec. They were P. cepacia, P. aeruginosa, Enterobacter, Flavobacterium and A. calcoaceticus. Most of them, except for P. cepacia were killed by the treatment of 0.1% CH for 5 min. P. cepacia was killed by 0.5% CH for 60 sec treatment. MIC of CH for those strains showed that chlorhexidine-resistant strain did not exist in the strains isolated from clinical material in the hospital where CH was routinely used as a general disinfectant. For a rapid and handy examination for the detection of disinfectantresistant bacteria, oxymeter was applied. Oxygen consumption of bacteria was inhibited at the same concentration of MIC which was examined by a conventional method, indicating that the method was applicable to a routine use. Discussion was made on a possible mechanism of chlorhexidine resistant.

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It was reported that fibronectin eyedrops facilitated healing trophic ulcer and recurrent corneal erosion.We studied effects of temperature, light, and material of container on the concentration of fibronectin.The decrement of fibronectin concentration at 4°C was less than that at room temperature and 37°C.When fibronectin was placed in dark, the concentration decreased very slowly.A strong adsorption of fibronectin on the wall of glass vessel was observed, while little on the polypropylene or polyethylene container.These results suggest that fibronectin eyedrop preparation should be kept at 4°C and in polypropylene or polyethylene container in dark.

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Adriamycin (ADM), an important antitumor agent with a wide spectrum, has dosage limitation in its clinical use because of its dose-related cardiotoxicity. One of the approaches to minimize this toxicity is to encapsulate ADM in liposomes.
We have developed an injectable liposomal preparation in which ADM is entrapped (ADMLip) for intrahospital use. ADM was entrapped in negatively charged liposomes which were composed of egg phosphatidylcholine, cholesterol, and phosphatidylserine (molar ratio of 5: 4: 1). Effective ADM entrapment (rate) in our intrahospital preparation was approximately 60%. The liposomal particle size, as measured by the dynamic light scattering method, was 203±90 nm. ADM-Lip was sterilized through a polycarbonate membrane filter with a pore diameter of 0.2μm.

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Oral amino acid preparations, enriched branched chain amino acids (BCAA) based on Fischer's theory have been used parenterally in hepatic failure with hepatic coma so far. Recently theoral powder form which was administered by dissolving in warm water was commercially developedand now widely used. But this preparation contains so much of bitter BCAA and haveto take it continuously over a long period that patients are suffering from the taste and largevolume of the solution prepared.
We tried to test here to improve the taste of the preparation by mixing many soft drinks commercially available as the flavoring agents. As the results, bitterness of the preparation was effectively suppressed by especially tasting sour and sweet with soft drinks. Also use of tubes similar to straws for soft drinks was useful to take much volume of the solution without tasting bitter.

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Compatibilities of five kinds of liquids in combination with Brocin^® Solution or Brocin^®-Codeine Syr., Conc.(Bro-Code) were investigated with regard to changes in appearance, redistribution and pH value, and high performance liquid chromatographic (HPLC) determination of the principal ingredients of five kinds of liquids, together with thin layer chromatographic (TLC) determination of Bisolvon admixture.
In the combination of Astomin^® Syrup, precipitation was observed, while in that of Pontal^® Syrup, decrease of redistribution was observed. In the combination of Zaditen^® Syrup, decrease of Ketotifen fumarate content was found, whereas it was prevented at 5°C.And in the combination of Bisolvon; Solution, decrease of Bromhexine hydrochloride content was found, whereas it was prevented only in that with Brocin Solution at 5°C. Furthermore in the determination with TLC, quantitative determination values of Bisolvon admixture were about the same as the results with HPLC. From the above results, it is indicated that Astomin Syrup and Pontal Syrup are imcompatible with Brocin Solution and Bro-Code, and Bisolvon Solution is imcompatible with Bro-Code.

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