Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
Volume 52 , Issue 2
Showing 1-25 articles out of 25 articles from the selected issue
  • Masaomi ONIKI, Akira OGOSHI, Takao ARAKI
    1986 Volume 52 Issue 2 Pages 169-174
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    One hundred fifty-seven Japanese isolates of Rhizoctonia solani Kühn AG-1 were examined to develop their perfect state by the soil-on-agar culture method. The medium effective for the pre-culture was PPYDA containing 0.5g dextrose, 5g peptone, 5g yeast extract, 20g agar, and 1, 000ml of 30g potato decoction. The optimum temperatures and humidities for the post-culture were in the range of 23-26 C and 80 to under 100%, respectively. Among 157 isolates of AG-1 tested, 6 of the cultural type IA, 1 of IB, and 2 of IC developed the perfect state. The perfect state of R. solani AG-1 was identified as Thanatephorus cucumeris (Frank) Donk based on the morphology and dimensions of the basidia, sterigma, and basidiospores.
    Download PDF (1082K)
  • Wang Hyu LEE, Akira OGOSHI
    1986 Volume 52 Issue 2 Pages 175-183
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    Using bacteria isolated from the surfaces of sugar beet roots, their antibiosis to sugar beet damping-off pathogens was tested, and highly antibiotic bacteria were selected. Out of 1, 094 bacterial isolates, 199 (18.2%) were antibiotic to Aphanomyces cochlioides and inhibition zones were formed between two organisms on media. Most of these antibiotic bacteria, which were also antibiotic to Pythium spp. and Rhizoctonia spp. were fluorescent pseudomonads, non-fluorescent pseudomonads or bacilli. These antibiotic bacteria were present on the root surfaces of sugar beets and could be isolated all over the cultural season. Sugar beet seeds were inoculated with the selected bacterial isolates and sown in paper pots. The result of seed bacterization with the antibiotic bacterial isolates showed that there were the bacteria which promoted the germination of seeds, increased the top and root weights of seedling, and/or prevented the damping-off. As damping-off of seedlings was caused mainly by Pythium spp. in paper pots, the effect of bacterization was important at early stage of seedlings in this case. Most of bacteria which were effective in promoting seed germination, increasing growth, and/or preventing damping-off were fluorescent pseudomonads.
    Download PDF (504K)
  • Chang Kyu KIM, Shizuo MOGI
    1986 Volume 52 Issue 2 Pages 184-192
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    Four different pre- and postdisposing temperature regimes 23/15 C·23/15 C, 29/21 C·29/21 C, 23/15 C·29/21 C and 29/21 C·23/15 C were evaluated for their effects on percent penetration, hyphal growth and disease development by the rice blast fungus, Pyricularia oryzae, in the leaf epidermis of a susceptible rice variety Aichi-asahi. Typical leaf blast lesions produced more in 29/21 C·23/15 C plot than in other temperature treatments, but total number of typical lesions decreased as the length of duration of treatments became longer. On the contrary, total number of typical lesions increased by the longer duration of treatments in 23/15 C·29/21 C plot. Despite of the increase in percent penetration until 120 hours after inoculation, no significant differences were found for percent penetration among temperature regimes, but hyphal growth in the leaf epidermis and number of cells in which hyphal growth was observed were greatly influenced by temperature regimes; i. e., the seedlings disposed at 29/21 C regime after completion of inoculation exhibited apparent hyphal growth since 96 hours after inoculation, while hyphal growth was suppressed until 120 hours on the seedlings postdisposed at 23/15 C regime. Thus, lesion formation on the seedlings disposed at lower temperature after inoculation was delayed due to the suppressed activities of blast fungus after penetration.
    Download PDF (768K)
  • Ichiro FUJISAWA
    1986 Volume 52 Issue 2 Pages 193-200
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    A potexvirus which had not been described previously, was isolated from Asparagus officinalis in Japan and the name asparagus virus III (AVIII) is proposed. AVIII infected 26 species from 8 families by sap inoculation, out of 37 species from 11 families tested. Artificially infected asparagus plants did not show any distinct leaf symptoms in the greenhouse for 2 years. The virus was not transmitted by aphids and through seeds of infected asparagus. Sap from infected Nicotiana megalosiphon was infective after heating for 10 min at 55 C but not at 60 C, after dilution to 2×10-4 but not 10-4, and after 21 days but not 23 days at 20 C. The virus particles were elongated flexuous rods, with a modal length of 580nm×13nm. Ultraviolet absorption spectrum of purified virus preparation was characteristic of that of nucleoprotein with an A 260/280 ratio of 1.18-1.19. The titer of the antiserum against the virus was 1/1024 in ring interface precipitin test. This antiserum reacted positively with AVIII but not with potato virus X(PVX) in SDS-agar gel immunodiffusion test. In tests with immune electron microscopy using the antibody decorating technique, the virus reacted with antisera against cactus virus X and narcissus mosaic virus but not with antisera against white clover mosaic virus, PVX, clover yellow mosaic virus and tulip virus X. All of the properties described are consistent with AVIII being a distinct member of potex-virus group.
    Download PDF (1134K)
  • Byung Kook HWANG, Wolf-Dieter IBENTHAL, Rudolf HEITEFUSS
    1986 Volume 52 Issue 2 Pages 201-208
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    The cultivar Peruvian of spring barley, which is susceptible at all growth stages, and Asse, which exhibits adult-plant resistance to powdery mildew, were compared in 14CO2 assimilation, distribution of 14C, and 14C-carbonate uptake in different organs of healthy and infected plants. The reduction of 14CO2 assimilation in infected plants at the first and fourth leaf stages was greater in Peruvian than in Asse. In Peruvian, the 14C which was fixed by the infected third leaf of plants with mildew on the lower 3 leaves remained in the third leaves with very little translocation to other parts of the plant. Infection of the lower three leaves at the fourth leaf stage reduced 14CO2 assimilation in noninfected fourth leaves of Asse less than that of Peruvian, but the flow of 14C from the healthy fourth leaves into other plant parts such as leaf sheaths was markedly stimulated in Peruvian compared to Asse. Infection also reduced the uptake of 14C-carbonate by seedling roots, the reduction being greater in Peruvian than Asse. A greater proportion of the 14C absorbed by roots of Asse was translocated to the infected leaves than that of Peruvian. It was concluded that powdery mildew disrupted the normal pattern of photosynthesis and translocation of metabolites in a susceptible cultivar more markedly than in an adult-plant-resistant cultivar of spring barley.
    Download PDF (490K)
  • Takio ICHITANI, Susumu TAKAMATSU, D. J. STAMPS
    1986 Volume 52 Issue 2 Pages 209-216
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    Three species of Pythium (P. okanoganense, P. vanterpoolii, P. volutum) were newly identified as pathogens of Pythium snow rot of wheat and barley in Japan. From the results on frequency of isolation and pathogenicity, it can be concluded that P. okanoganense is an important pathogen, like P. iwayamai and P. paddicum reported previously by other authors, but both P. vanterpoolii and P. volutum are minor.
    Download PDF (1689K)
  • Shigeo NAITO, Toshiya SUGIMOTO
    1986 Volume 52 Issue 2 Pages 217-224
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    In August of 1983, a previously unreported Sclerotinia stalk rot was first observed on sugar beets at the flowering stage in Sapporo, Japan. The symptoms appeared as dark green to light brown discoloration with irregularly shaped, soft rot lesions on the flowers, stems and leaves. In addition, the symptoms were characterized by the development of whitish cottony mycelia and the formation of large, black-colored scierotia on the surface of the diseased plants. Sclerotinia sp., consistently isolated from the diseased stalks, caused severe stalk rot of sugar beets or stem rot of kidney bean plants when these plants were inoculated artificially with the fungal ascospores at the flowering stage in a moist chamber. However, in the inoculation before the flowering stage, fungus did not cause any lesion on sugar beets or kidney beans. Maximum mycelial growth of the causal fungus on nutrient agar plate was at 25 C, while hyphae grew only slightly at 30 C. Isolates of Sclerotinia sp. from sugar beets and S. sclerotiorum from kidney bean plants were found to be compatible and intermingled freely without the formation of a brown incompatibility zone in the regions of association in dual culture on agar medium. The fungus was morphologically and physiologically identified as Sclerotinia sclerotiorum (Libert) de Bary.
    Download PDF (1202K)
  • Hiroshi YAEGASHI, Masao YAMADA
    1986 Volume 52 Issue 2 Pages 225-234
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    A total of 130 isolates of Pyricularia oryzae from 6 countries were tested for the identification of pathogenic race and mating type. The number of races identified from country of origin was, Soviet Union-3 Japanese races [3 international races], China-7 [9], Nepal-2 [2], Thailand-3 [2], Indonesia-4 [4], and Colombia-5 [12]. The reaction of these races identified by means of Japanese and international rice cultivars did not always correspond. About 40% of the isolates used were of untetermined pathogenic race, because of inconsistent host reaction. The phenotypic variability in pathogenic reaction could be due to the heterogeneity of the differential rice cultivars as well as to the pathogenic change of the blast fungus itself. The presence of both mating types was confirmed in the isolates from China and Colombia, but in the Russian and Nepalese collection mating type A was only detected. On the other hand, all of the Indonesian isolates used were of mating type a.
    Download PDF (463K)
  • Hideo ABE, Tetsuo TAMADA
    1986 Volume 52 Issue 2 Pages 235-247
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    Seven isolates of Polymyxa betae with a different host range were compared with respect to their association with beet necrotic yellow vein virus (BNYVV). When 14 species of plants were inoculated with the fungus isolates under greenhouse conditions, transmission of BNYVV was related to the fungal isolates from sugar beet, spinach and Chenopodium murale which were infective to sugar beet. But the isolates from C. album, Amaranthus retroflexus and Portulaca oleracea which were not infective to sugar beet did not carry the virus. Viruliferous sugar beet isolates became virus-free after being propagated in the roots of C. ficifolium. When these virus-free resting spores were propagated in the roots of sugar beet infected with BNYVV by manual inoculation, the fungus again acquired and transmitted the virus to healthy plants. Infectivity of BNYVV was maintained together with the survival of P. betae in air-dry and moist soil for fifteen years. The virus infectivity was also retained in zoospores of P. betae treated with BNYVV antiserum and resting spores treated with virus antiserum, HCl or NaOH. In ultrathin sections of sugar beet rootlets infected with the viruliferous sugar beet isolate of P. betae, the virus particles were usually encountered in the cytoplasm in contact with the outer plasmodial wall of the infected cells, and were also present in the vacuoles and the protoplasm of immature zoospores of the fungus. No virus particles were observed in resting spores. The virus, however, was detected by ELISA in the extracts of viruliferous resting spore clusters isolated from root tissues. From these results, it is concluded that BNYVV transmission is depending on strains of P. betae and the plant species which the fungus proliferates. It is suggested that BNYVV remains in P. betae for a long period of time but does not multiply in the fungus.
    Download PDF (3043K)
  • Shaw-Ming YANG
    1986 Volume 52 Issue 2 Pages 248-252
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    A previously unknown race of Puccinia helianthi was isolated from a commercial field of sunflower at Bushland, Texas, in 1984. Sunflower rust differential lines and cultivars that are resistant to the four North American rust races, the ten rust races of Antonelli, and the four rust races of Luciano and Luciani from Argentina were susceptible to this newly isolated race. The five sunflower germplasm lines, HA-R1, HA-R2, HA-R3, HA-R4 and HA-R5 that had been released as resistant to the four North American rust races were also susceptible to this new race. The results showed that this isolate is a new race of the sunflower rust pathogen.
    Download PDF (960K)
  • Seiya TSUSHIMA, Satoshi WAKIMOTO, Shizuo MOGI
    1986 Volume 52 Issue 2 Pages 253-259
    Published: April 25, 1986
    Released: May 26, 2009
    JOURNALS FREE ACCESS
    A selective medium (S-PG) was developed for isolation of Pseudomonas glumae causing the disease of bacterial grain rot of rice. The composition of the medium was as follows: KH2PO4 1.3g, Na2HPO4 1.2g (NH4)2SO4 5.0g, MgSO4·7H2O 0.25g, Na2M0O4·2H2O 24mg, EDTA-Fe 10mg, L-cystine 10μg, D-sorbitol 10g, pheneticillin potassium 50mg, ampicillin sodium 10mg, cetrimide 10mg, methyl violet 1mg, phenol red 20mg, agar 15g in one liter of distilled water. P. glumae and some other pseudomonads grew well on S-PG medium to develop characteristic colonies, but most of the other microorganisms tested were inhibited. P. glumae formed two differnt types of colonies, A and B, depending upon the isolate. Colony forming efficiency of P. glumae on S-PG medium was higher than those on semisynthetic potato agar, nutrient agar and yeast peptone dextrose agar. Among the other bacterial isolates tested, only P. avenae produced colonies similar to type B. All of the 37 isolates of P. glumae tested produced specific precipitin band with the antiserum of P. glumae isolate Kyu82-34-2 in agar gel diffusion test, but none of the isolates of P. avenae did. These results suggest that P. glumae will be detectable from natural specimens by applying S-PG medium combined with serological method.
    Download PDF (1874K)
  • Yoshio EHARA, Gaylord I. MINK
    1986 Volume 52 Issue 2 Pages 260-269
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    The infectivity of cucumber mosaic virus Y strain (CMV-Y) decreased after mixing with bovine pancreatic ribonuclease (RNase). The amount of infectivity lost was dependent on the virus to enzyme ratio in the mixture and on the pH used for incubation. The enzyme seemed to bind at random to the surface of virus particles. Binding was partially reversed by pH adjustment. Most of the RNase which attached to the virus at pH 6 appeared to be released at pH 8. As the proportion of RNase in mixtures increased, the virus particles and enzyme formed complex aggregates. Degradation of virions occurred only at very high RNase concentrations. Degradation occurred more rapidly at pH 6 than pH 8. The infectivity loss upon addition of small amounts of RNase appeared not to be caused by aggregation or virion degradation. It is suggested that RNase attached to the virus prevents some processes necessary for initiation of infection or attacks the uncoated RNA.
    Download PDF (441K)
  • Toshihiro OMURA, Yoshiyuki TAKAHASHI, Kenichiro SHOHARA, Yuzo MINOBE, ...
    1986 Volume 52 Issue 2 Pages 270-277
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    Hybridoma cells that secrete monoclonal antibodies against rice stripe virus (RSV) were produced by fusing spleen cells of a BALB/c mouse immunized with RSV to a mouse myeloma cell line P3-X63-Ag8-U1 (P3U1). A single stable cell line with the highest titer in the avidin-biotin-peroxidase system to the homologous antigen was screened. The titer of the hybridoma ascitic fluid increased with the number of days after the injection of hybridoma cells to mice. The titers of the ascitic fluid were 800-1, 600 in precipitin ring interface test. The yield of the ascitic fluid was 11.4-22.5ml for a single mouse and was ca. 100ml for 6 mice. The titer and the yield of the ascitic fluid decreased with the serial passage of the hybridoma to new mice and the titers became about half that of the initial value after one to two passages through mice. The monoclonal antibodies were successfully used to detect virus antigen in virus infected plants and viruliferous insects by the latex flocculation test and enzyme-linked immunosorbent assay.
    Download PDF (399K)
  • Yuji NAGAI, Masanobu FUKAMI, Akio MURATA, Tsuneo WATANABE
    1986 Volume 52 Issue 2 Pages 278-286
    Published: April 25, 1986
    Released: May 26, 2009
    JOURNALS FREE ACCESS
    Blot-like lesions have occurred on taproots of carrots in summer-and winter-maturing crops in Chiba Prefecture for past 10 years, resulting in significant quality and yield losses. Pythium sulcatum (ref. (2) in the same title) was isolated from these diseased roots in various localities of Chiba Pref. The disease was severe in the fields with repeated cultivation of carrots, especially in the years with excessive rainfall prior to the harvest. The fungus was only pathogenic to the roots of carrots, but it did not cause damping-off nor leaf blight. In the summer-maturing crop, water-soaked brown circular or elliptical lesions, 3-5mm in diam, were formed mainly on taproots at harvest, and often soft-rotted on the surface. In the winter-maturing crop, small brown slightly sunken spots, 2-3mm in diam., accompanied occasionally with vertical cracks were formed on the taproots. In both cropping types, lateral roots were discolored to brown and decayed frequently and these roots got off from the taproots. Variations of the symptoms may be due to differences of cultivars, time of the disease occurrence and soil conditions.
    Download PDF (1086K)
  • Tsuneo WATANABE, Yuji NAGAI, Masanobu FUKAMI
    1986 Volume 52 Issue 2 Pages 287-291
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    A Pythium species (isolates A-1 and A-4) obtained from diseased carrot roots with water-soaked brown blot-like symptoms in Chiba Prefecture is slow in growth rate and it has specific pathogenicity only limited to carrot roots. It is morphologically characterized by formation of globose, subglobose or peanut-shaped hyphal swellings and predominantly single monoclinous clavate, crook-necked or various-shaped antheridia with transverse furrows and folds. This fungus was identified as P. sulcatum Pratt and Mitchell based on morphological and physiological characteristics.
    Download PDF (1015K)
  • Katsumi AKUTSU, Tomoyuki KOMAGATA, Satoshi OKUYAMA
    1986 Volume 52 Issue 2 Pages 292-301
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    Number, size, shape and distribution of nuclei in hyphal tip cells of Botrytis cinerea treated with inosine were different from those in the untreated ones. In the presence of inosine, the nuclei increased remarkably, and nuclei which differed in size and shape were contiguous in the hyphal tip cells and in the nuclei with one or two budding-like projections were frequently observed in these cells. In the hyphal tip cells treated with inosine, the nuclei lined up in one parallel or diagonal row, although the nuclei distributed in a file more or less uniformly in the untreated ones. From electron microscopy of the hyphal tip cells treated or untreated with inosine, the nuclei of various stages in mitosis were observed in the untreated ones, while in the treated ones, deformation of the nuclei suggesting non-mitosis, such as budding was frequently observed: nuclei whose part of the nuclear envelope began to project out, formed developing projections or the projections appeared nearly detached from the parent nuclei. In these nuclei, the spindle pole bodies and microtubules, which took roles in mitotic division of nuclei, were not found.
    Download PDF (2466K)
  • Manabu KUSUNOKI, Kaoru HANADA, Mitsuro IWAKI, Moo Ung CHANG, Yoji DOI, ...
    1986 Volume 52 Issue 2 Pages 302-311
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    A new disease of cycas (Cycas revoluta) characterized by dwarfing and twisting of young leaves and chlorotic or necrotic spots on mature leaves was shown to be caused by a small spherical virus of 28nm in diam. By sap inoculation the virus was easily transmitted to some Chenopodium spp. in which chlorotic local lesions and systemic mottlings were produced. The host range of the virus was limited. Out of 39 species in 12 families tested, only ten species in Aizoaceae, Amaranthaceae and Cycadaceae were susceptible to the virus. The virus was transmitted through seeds from diseased plants of C. amaranticolor and C. serotinum at the rate of 29 and 82 per cent, respectively. The virus was inactivated after 10min at 60-65 C, and 18-24 days at room temparature. The dilution end point lay between 1×10-3 and 5×10-3. The virus particles were observed to show a linear arrangement in tubular structures in both cytoplasm and plasmodesmata, and to form crystalline aggregates in cytoplasm. The virus was purified from systemically infected C. quinoa leaves, and the purified virus was separated into three components after sucrose density gradient centrifugation. The properties of the virus were very similar to those of nepoviruses so far described, but no serological reaction was found between the virus and nine known nepoviruses tested. The virus was concluded to be a new member of nepoviruses, and was named the cycas necrotic stunt virus. This disease designated as the cycas necrotic stunt disease was newly described.
    Download PDF (3996K)
  • Tsuneo NAMAI, Maki ENDO, Masahiro YUSA, SUSUMU YAMANAKA, Yoshio EHARA
    1986 Volume 52 Issue 2 Pages 312-319
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    Two blast isolates which were obtained from ragi, Eleusine coracana (L.) Gaertn., one isolate from goosegrass, Eleusine indica (L.) Gaertn., and one isolate from Italian millet, Setaria italica (L.) Beauv. produced perithecium-like structure (PLS) in single culture on agar medium. Goosegrass isolate (915) and ragi isolate (TEST A-11) produced numerous PLSs on oatmeal agar medium and on two kinds of oatmeal media containing yeast extract. While, Italian millet isolate (1281-2) produced it only on oatmeal media containing yeast extract. However, neither ascus nor ascospore were observed in any of these PLSs. Since all the isolates produced PLS in this study formed 2 bands of perithecia along the junctional line of both colonies by mating between compatible isolates, then, all 4 isolates functioned as hermaphrodities. Additionally, the process of PLS development of goosegrass isolate (915) and ragi isolate (TESTA-11) were indistinguishable with the productive process of perithecium by mating between compatible isolates, goosegrass isolate (915) and ragi isolate (902), except the fact that ascus and ascospore were not formed in the peridium.
    Download PDF (1405K)
  • Masayuki SANADA, Kamehisa MATSUSHITA, Hidetoshi SHIMOKAWA, Riyoko ITOH
    1986 Volume 52 Issue 2 Pages 320-329
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    The β-1, 3-glucan hydrolase activity increased in the leaves of Nicotiana glutinosa and N. tabacum var. Xanthi-nc inoculated with common strain of tobacco mosaic virus (TMV-OM) and in White Burley leaves inoculated with tomato strain of the same virus (TMV-T), but not in the leaves of White Burley and Xanthi inoculated with TMV-OM. In the experiment of artificial necrotization by treatment with hydrochloric acid and heated platinum ring, the increase of the activity was not recognized. On localization of the activity in the leaves of N. glutinosa inoculated with TMV-OM and of White Burley inoculated with TMV-T, the lesion areas showed higher activity than the marginal tissues around the lesions. When the inoculated detached leaf-halves of N. glutinosa were first incubated on distilled water at 25 and 35 C, then transferred to 25 C, a marked increase of the activity and formation of local lesions were recognized. However, when the same leaves were incubated continuously at 35 C, no increase of the activity and no local lesion were observed. Furthermore, in detached leaf-halves of N. glutinosa which were inoculated with TMV-OM and incubated on distilled water in dark and on 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea (DCMU) solution under light at 25 C, the dark- and DCMU-treatment induced the decrease of the activity and of formation of local lesions. However, such effect was not observed in the incubation on the glucose solution in dark. Using the polyacrylamide gel electrophoresis, the band of the enzyme from both TMV-OM-inoculated and noninoculated leaves of N. glutinosa, Xanthi and White Burley was single, but that from Xanthi-nc inoculated with TMV-OM and from White Burley inoculated with TMV-T was separated into two bands with a new additional one. In Nicotiana plants formed local lesion with inoculation of TMV strains, the enzyme activity was increased specifically. There was a change in the electrophoregram of the enzyme among the Nicotiana plant-TMV strain combinations.
    Download PDF (1141K)
  • Masatoshi FUJIWARA, Hachiro OKU, Tomonori SHIRAISHI, Seiji OUCHI
    1986 Volume 52 Issue 2 Pages 330-335
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    The effect of pruning of organs on the induction of the systemic resistance in barley seedling against powdery mildew fungus was investigated. Pruning of leaves, root, and remaining albumen of barley seedling induced resistance in the remaining leaves against the infection of powdery mildew fungus. The resistance was induced within 2 hr after pruning of organs and continued at least for 6 hr, in some cases for 120 hr. The pruning of organs did not reduce the hyphal growth of the challenger. The pruning of root system with hypocotyl did not induce resistance, but induced rather susceptibility in the primary leaf.
    Download PDF (290K)
  • Wen-hsiung KO, Richard K. KUNIMOTO
    1986 Volume 52 Issue 2 Pages 336-337
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
  • Hiroyuki MUKOBATA, Kiyonobu NAHATA, Takashi YAMAMOTO
    1986 Volume 52 Issue 2 Pages 338-342
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    Stem blight of tulip in Japan was first found in Toyama Prefecture in late-April, 1982. The diseased plants stagnated and their stems were covered with light pink mycelia in which orange sporodochia appeared. A Fusarium fungus isolated from the diseased plants was proved to be causal agent of the disease and it also showed pathogenicity to barley in inoculation tests. The causal fungus was identified with Fusarium roseum f. sp. cerealis ‘Avenaceum’ by its morphological characteristics.
    Download PDF (1469K)
  • Mamoru SATO
    1986 Volume 52 Issue 2 Pages 343-346
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
  • Hachiro OKU, Tomonori SHIRAISHI, Seiji OUCHI
    1986 Volume 52 Issue 2 Pages 347-348
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
  • Tomio USUGI, Shoji YAMAMOTO, Tsuneo TSUCHIZAKI
    1986 Volume 52 Issue 2 Pages 349-354
    Published: April 25, 1986
    Released: February 19, 2009
    JOURNALS FREE ACCESS
    A sap transmissible virus was isolated from satsuma mandarins showing ringspots on fruits in Arida district of Wakayama prefecture. Symptoms of herbaceous plants inoculated with the virus were slightly different from those of satsuma dwarf virus (SDV). The virus particles were polyhedrons of approximately 28 nm in diameter and consisted of three centrifugal components. Double immunodiffusion and cross-absorption tests showed that the virus was heterologously related to SDV. Satsuma mandarins inoculated with the purified virus developed typical ringspots on fruits.
    These results indicate that the virus is citrus mosaic virus causing mosaic diseases in satsuma mandarins.
    Download PDF (966K)
feedback
Top