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Volume 71 (2005)
- Issue 4 Pages 307-
- Issue 3 Pages 139-
- Issue 2 Pages 91-
- Issue 1 Pages 1-
Volume 71, Issue 1
Displaying 1-10 of 10 articles from this issue
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K. TSUDA, Y. KOSAKA, T. KOBORI, H. SHIOMI, K. MUSUMI, M. KATAOKA2005 Volume 71 Issue 1 Pages 1-5
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSAfter tomato plants were inoculated with an attenuated isolate CM95 of Cucumber mosaic virus (CMV), a promising biocontrol agent for CMV, either the number or weight of marketable fruits was reduced when a basal level of fertilizer was added to rock wool or soil culture in aphid-proof plastic houses. However, increasing the amount of fertilizer when applied as a solution or side dressing hardly reduced yield while significantly increasing vitamin C in tomato fruits.View full abstractDownload PDF (836K) -
K. MANABE, N. TASHIRO, H. NEGISHI, K. SUYAMA, N. MATSUYAMA2005 Volume 71 Issue 1 Pages 6-10
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSNew benchmarks were developed for differentiating Streptomyces scabies, S. acidiscabies and S. turgidiscabies, that cause potato common scab. Secondary metabolites of the pathogens were extracted directly with methanol-diethylether (2:1, v/v) or acetonitrile from a solid Bennett's medium after 5 days of culture at 25°C. The extracts were spotted onto a precoated silica gel TLC plate (Merck Co., Si60, 0.25mm), which was then developed at 25°C for 90min, with chloroform-methanol-acetone-acetic acid-distilled water (55:20:4:4:2, v/v). The characteristic spots were detected by UV irradiation at 365nm, and the resultant chromatograms were recorded with a digital camera and analyzed with a personal computer. Because chromatograms are specific, three causal agents of potato common scab disease can be rapidly differentiated.View full abstractDownload PDF (2884K) -
T. ASHIZAWA, K.S. ZENBAYASHI2005 Volume 71 Issue 1 Pages 11-15
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSThe method of Goto and Yamanaka (1968) was modified to improve efficiency when differentiating of Pyricularia grisea races. Three seeds of one of each of 12 Japanese differential rice varieties were sown in a 3-cm square cell of a 12-cell tray (3×4 cell) and treated immediately with 10ml of 2.5μg/ml uniconazole (a plant growth regulator) to yield dwarf seedlings. The seedlings (5-leaf stage) were then inoculated with one of 23 blast isolates (6 races), and covered with plastic cases to prevent contamination from the other isolates. The blast races identified by the method were consistent with those identified with the method of Goto and Yamanaka. With the use of dwarf seedlings and protection cases, many blast isolates can be identified at the same time.View full abstractDownload PDF (5002K) -
T. ASHIZAWA, Y. KATAOKA2005 Volume 71 Issue 1 Pages 16-19
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSUstilaginoidea virens, which causes false smut of rice, on panicles of rice cultivar Toride 1 cultivated in the field was detected before and after heading using a nested-PCR technique with a two-species-specific primer set. The fungus was detected in rice panicles in both inoculated and noninoculated plots at the booting stage, although the frequency of the detection tended to be less than that detected at first and full heading. This technique is effective for detecting the fungus, and the panicles were found to be infected with the fungus before heading.View full abstractDownload PDF (2788K) -
H. SHINOHARA, K. UEHARA, I. KADOTA, K. NISHIYAMA, S. TSUSHIMA2005 Volume 71 Issue 1 Pages 20-22
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
JOURNAL FREE ACCESSA new bacterial wilt was found on balsam pear (Momordica charantia L.) in Okinawa, Japan in October 1999. The causal bacterium was identified by its bacteriological characteristics as Ralstonia solanacearum, and the name “bacterial wilt” was proposed for the disease.View full abstractDownload PDF (2391K) -
2005 Volume 71 Issue 1 Pages 23-30
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
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2005 Volume 71 Issue 1 Pages 31-43
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
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2005 Volume 71 Issue 1 Pages 44-51
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
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2005 Volume 71 Issue 1 Pages 52-80
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
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2005 Volume 71 Issue 1 Pages 81-85
Published: February 25, 2005
Released on J-STAGE: February 19, 2009
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