原生動物学雑誌
Online ISSN : 2189-6747
Print ISSN : 0388-3752
42 巻 , 1 号
選択された号の論文の2件中1~2を表示しています
Original
Short Communication
  • Takeshi Yonemichi, Hiro Yasukawa
    2009 年 42 巻 1 号 p. 13-16
    発行日: 2009年
    公開日: 2017/09/09
    ジャーナル フリー

    Dictyostelium discoideum amoebae grow as unicellular microorganisms until nutrients are depleted, while they aggregate and form multicellular structures under a starved condition. We established D. discoideum expressing a green fluorescent protein (GFP) under control of an upstream sequence of the gene for RecA, a mitochondria-localizing DNA repair enzyme. Multi-cellular structures formed by the strain were irradiated by 50 J/m2 UV light (254 nm) and GFP expression was analyzed. Fluorescence of GFP was observed not in the prestalk cells but in the prespore cells in the multicellular structures. The prestalk cells are programmed to die, while the prespore cells differentiate into viable spores. D. discoideum is thought to repair DNA damage in the prespore cells and maintain genomic integrity using a limited energy source.

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