We found and reported cases of allergic contact dermatitis among growers of ornamental chrysanthemums. This dermatitis occurs in parts of the skin that come in contact with chrysanthemums during cultivation and forwarding before the flowering. The occurence ratio of this dermatitis is decreasing. Its ratio in 1976 was 23.1% on the men and 44.4% on the women, but, in 1982 it was 10.7% and 23.0%, respectively. We suspect that three causes were effective in it. The first one and the second one were caused by changing of the kinds of chrysanthemums and moldifying the cultivation method. The third one was done by the device to block direct contacts between chrysanthemums and the skin as much as possible for protection. While, the positive ratio of patch test on growers with the crude extract of fresh leaves of the chrysanthemum is increasing. In 1976, it was 27.3% and in 1982, it was 43.3%. We made patch tests on the farmers with the crude extracts of the leaves and the samples that were extracted from fresh leaves of “Chrysanthemum morifolium Ram.” (cultiva “Kinriki”). Also, as one of these test samples, we used “Fn-1”, a kind of sesquiterpene lactone, that extracted from Frullania nodulosa. These results suggest that there are two types of allergens of “Kinriki”. One type is soluble in organic solvents and includes such ones as sesquiterpene lactone. The other is water soluble compounds, probably including peptides or proteines. We found that of two allergens, the probability that sesquiterpene lactone acts as allergens is 55%, the probability that the protein acts as allergen is 15% and the probability that both are effective is 20%.
A hot water extract of Flammulina velutipes Sing.(FEH-1) showed a markedly high antitumor effect against sarcoma 180 in ICR mice. FEH-1 (10mg/kg) was administered intraperitoneally for consecutive 21 days before and after the subcutaneous inoculation of sarcoma 180 (4.1X106 cells) to the mice. Animals were sacrificed on the 24th day after the inoculation of sarcoma 180 and tumor weight was determined. The highest tumor inhibition ratio was 98.5%, when FEH-1 was administered at the dose of 10mg/kg. Cytotoxicity of FEH-1 was denied by the following two tests:(1) after mixed-culture of Vx2 tumor cells with FEH-1 for 7 days in vitro, number of Vx2 cells was counted by dye-exclusion technique, and (2) after contact of Vx2 cells with FEH-1 for 1 hour, the cell mixture was inoculated subcutaneously to the rabbits. It was suggested that antitumor effect of FEH-1 was host-mediated.
During the 18-month period from January 1, 1981 to June 30, 1082, we encountered a total of 11 cases of child peptic ulcer-three gastric ulcer and eight duodenal ulcer casen. There is every indication that the incidence of child peptic ulcer will increase in a rural area like Yuri, which is situated in Japan's northeastern prefecture of Akita. To be noted is the fact that 10 cases out of 11 of peptic ulcer were found in three-year lower secondary schoolchildren and the remaining one was found in a sixth grader of six-year elementary school. The incidence as well as the number of visits to our clinics went up as the age advances and reached a peak in third graders of lower secondary school. The reason why third graders of lower secondary school were attacked most by peptic ulcer is probably that they, at puberty, are under psychic or psychogenic stress with anxiety over high school entrance examinations, mental strain from forced attendance at a cramming school, trouble in getting along with friends, and dissatisfaction with parents. Therefore, child peptic ulcer should not be treated as a disease of the digestive organ alone but as a disorder in the autonomic nervous system with an aid of psychosomatic medicine and psychiatry. For the prevention of the disease, it would also be necessary to take account of socio-cultural factors.