We compared histochemical characteristics and their changes during hindlimb unloading (HU) among proximal, middle, and distal regions (10%, 50%, and 90% of muscle length from the proximal end, respectively) along the longitudinal axis of the plantaris muscle in adult rats. Twenty-two female Fischer 344 rats (6 months old) were assigned to either the control (
n=7) or HU (
n=15) group. Rats of the HU group had their hindlimbs suspended for 1 (
n=7) or 3 weeks (
n=8). Muscle wet weight, water content, fiber types (IIB, IIA, and I), and cross-sectional area (CSA) were measured. Fiber types were identified in serial sections stained with myofibrillar ATPase techniques. In control rats, the proximal region had the greatest percentage of type IIB fibers (89.1±5.4%, mean±SD), followed in descending order by the distal (80.2±3.2%) and middle regions (68.7±4.2%) with concomitant lesser percentages of type IIA and I fibers. The relative contribution of type IIB fibers to cross-section was greater in the proximal and distal regions than in the middle region with a lesser contribution of type IIA and I fibers. The CSA of type IIB fibers gradually increased from the proximal to the distal region. The CSA of type IIA and I fibers was greatest in the middle region. HU resulted in a 21.5% loss of muscle weight after 1 week and a 27.1% loss after 3 weeks compared with control, although water content did not change. Type IIB fibers began to atrophy after 1 week of HU, and type IIA and I fibers began to atrophy after 3 week of HU in the middle and distal regions. In contrast, the CSA of no fiber types in the proximal region changed significantly with HU. No change was observed in fiber type distribution or relative contribution at any region with HU. These results demonstrate that 1) the histochemical characteristics of fiber type compositions and CSA differ along the longitudinal axis of the muscle and 2) the degree of muscle fiber atrophy is affected by fiber types, duration of HU, and longitudinal region.
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