In the previous papers, changes in CO₂ tension and H⁺ concentration in the cerebrospinal fluid have been investigated with interest since those are known as stimulants on the central chemoreceptor. It has been thought that the changes in acid-base and gas tensions in the cerebrospinal fluid may be resulted from the alterations in those of arterial blood and the brain in relation to gas diffusion across the blood-brain barrier and blood-CSF barrier. In the present experiments, an investigation has been carried out to clarify the relationship between the changes of pH and gas composition of CSF and those of arterial blood and cerebral venous blood induced by inhalation of different gas mixtures.
Ten dogs (8-12kg) were anesthetized with chloralose (80mg/kg) one hour after recieving morphine (1mg/kg). Arterial blood samples were taken from the femoral artery, cerebral venous blood from the dorsal sagittal sinus and CSF from the cisterna magna. In the first series of the experiments, following a preliminary period of spontaneous breathing of room air, five dogs were made to breath a gas mixture containing 24%O₂, 10%CO₂ and 60%N₂ for twelve minutes, and then the samples were taken simultaneously at intervals of two minutes during the CO₂ inhalation. The sampling was continued until a recovery from the effect of CO₂ breathing had been reached after returned to room air breathing. In the second series of the experiments, five dogs were made to rebreath 100 liters of high O₂ mixture (40%O₂+60%N₂) and the samples were drown at intervals of ten minutes for two hours. All samples were taken with 0.1ml heparinized glass microcapillary and analysed by the electrode method (IL-113, type S-1).
In both series of the experiment, similar patterns of alterations in CSF P_CO2, and in the cerebral venous blood P_CO2, were obtained. However, the CSF P_CO2, never equilibrated with the cerebral venous blood P_CO2. within the experimental period. These findings lead us to suspect that the alteration in CSF P_CO2, may be related to the metabolism of brain. There was some-what a similarity of the patterns of alterations in CSF P_O2 and cerebral venous blood P_O2 in the first series. However in the second series, CSF P_O2 increased following an abrupt rise in arterial P_O2 and kept that level in parallel to the arterial P_O2 throughout the high O₂ breathing, but the cereral venous P_O2 did not rise. One hour later from the beginning of the high O₂ inhalation, cerebral venous blood P_O2 began to rise gradually, probably in response to the increased cerebral blood flow, but the CSF P_O2 remained in the some range as it was, regardless of the increased cerebral venous P_O2.
From these findings, it appears that there are some differences in mechanisms of regulation for the cerebrospinal fluid P_CO2 and _O2. Alteration of pH in CSF also appeared to be similar to the change of cerebral venous blood pH, but further investigations are required.

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Serum sialic acid, and index of serum glycoprotein, was estimated in 31 sets of paired sera gotten either in acute or in convalescent phase from the patients with influenza A₂, in 23 sets of paired sera gotten before and 21 days after the vaccination from the volunteers with the injection of a polyvalent influenza vaccine, and in 31 sera of the healthy persons.
At the same time, serum total protein, serum protein fractions and A/G ratio were measured and compaired with sialic acid level.
The hemagglutination inhibition test (HAI) and the complement fixation test using soluble type-specific antigen of influenza virus A (SCF) were carried out in all the paired sera for ascertaining the effects of infection and vaccination.
Blood was collected before lunch time in order to avoid the influence of diet. All the sera gotten from the bloods were kept at -20°C till the beginning of the procedure.
Sialic acid was measured by using sulfuric acid, sodium sulfate, sodium periodate, sodium arsenite, thiobarbituric acid, isoamyl alcohol and cyclohexanone, and by means of a Leitz-photometer with a filter of 550μm. Total protein was estimated by a refractometer of Hitachi Co. Serum fractions were measured electrophoretically by means of cellulose acetate membrane technique and a self-recording densitometer of Kayagaki Co.
Almost all the influenza patients had been already vaccinated with influenza vaccine. And so, the true effects of the influenza infection on serum protein are not shown by direct reading of the results obtained from such patients. That is the reason why the vaccinated healthy persons were used as a control group, too.
The results obtained are as follows:
1. On the influenza patients.
a) Compared with the healthy control, sialic acid level was higher either in acute or in convalescent phase, and was higher in acute phase than in convalescent phase.
b) Compared with the control, α₁-globulin was of higher level in acute phase, α₂-globulin was higher either in acute or in convalescent phase, and albumin was lower in both phases. Compared with acute phase, α₁-globulin and α₂-globulin were decreased, and albumin was increased in convalescent phase.
c) A positive correlation was found between sialic acid level and α₂-globulin level in convalescent phase. Such a correlation was not shown as to α₁-globulin or acute phase.
d) Total protein, β-globulin and γ-globulin remained unchanged either in acute or in convalescent phase, and the relation between each of them and sialic acid was not demonstrated.
2. On the vaccinated healthy persons.

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One early lung scar cancer was accidentally discovered at postmortum examination on 72-year-old male who had myocardial infarction.
The scar cancer was located on the left superior segment subpleurally, so called hiluspetal type in form, and measuring 25×20×9mm in the greatest diameter. On microscopic examination, precancerous proliferative changes such as atypical metaplasia or adenomatous proliferation of the dilatated bronchiolar epithelium were found in the scar, and the tumor was characteristically adenocarcinoma of the bronchiolo-alveolar type.
The etiology of the scar is unknown, but pulmonary infarction is possibly considered. Furthermore, the pathological criteria of the scar cancer, frequency, type and histological pattern especially on relationship with terminal bronchiolar carcinoma were discussed and some literatures were reviewed.

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