日本臨床細胞学会雑誌
Online ISSN : 1882-7233
Print ISSN : 0387-1193
ISSN-L : 0387-1193
16 巻, 1 号
選択された号の論文の17件中1~17を表示しています
  • 上井 良夫, 鈴木 隆一, 山本 真司
    1977 年 16 巻 1 号 p. 1-7
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    This study was undertaken to develop an automated prescreening algorism for cervical cytology of squamous cell carcinoma of the uterus. Routine, vaginal smears stained with the Papanicolaou method were used. The squamous cells were classified into normal and abnormal ones. The former included normal squamous cells and those with a slight degree of atypia which appeared in inflammation and mild dysplasia. The latter included those from lesions worse than a moderate degree of dysplasia. By using a linear discriminant function with nuclear area (NA) and N/C ratio as parameters, the misclassification rate of abnormal cells resulted in 4.6%, when that of normal ones was 50%.By adding nuclear mean density (NMD) to these parameters, the former resulted in 3.3%, when the latter was 50%.Next, squamous cells were classified into normal, abnormal and unclassified ones with NA and N/C ratio and the unclassified cells were reclassified into normal and abnormal ones with NA and NMD (Hierarchic classification algorism). The misclassification rate of abnormal cells resulted in 3.3%, when that of normal ones was 28%.Applying “Nuclear Area with High Density (NAH) ”, instead of NMD, to the hierar chic classification algorism, the former resulted in 2.3%, when the latter was 15% (Cell type independent hierarchic classification algorism). The NAH is a new parameter defined as a nuclear area denser than mean value for minimal and maximal density and is considered to be related to the chromatin pattern. For further improving the result, the unclassified cells were classified in basal, parabasal and intermediate types with NA and N/C ratio and, in each type, they were reclassified into normal and abnormal cells with NA and NAH (Cell type dependent hierarchic classification algorism). The misclassification rate of abnormal cells resulted in 1.7%, when that of normal ones was 6.7%. Thus, the best result was obtained by the cell type dependent hierarchic classification algorism.
  • 杉下 匡, 天神 美夫, 山本 久美夫, 五十嵐 優子, 西浦 天宣, 横山 哲也, 大村 峯夫, 寺嶋 章晃, 高橋 俊仁, 覧村 和夫, ...
    1977 年 16 巻 1 号 p. 8-16
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    A requirement necessary for automated cytological screening is the development of “segmentation” and “clustering”.
    The study specimen consists of a magnified image with 400 spots. The image is scanned by an “image processing system” with density values for each spot recorded on a data recorder. These density values are evaluated by a MELCOM 7700 computer.
    The image processing system contains a NOVA Model 2 mini computer.Segmentation consists of converting the density values of the 400 spots into a histogram, from which individual cells are selected.
  • 山本 久美夫, 五十嵐 優子, 杉下 匡, 天神 美夫, 大村 峯夫, 西浦 天宣
    1977 年 16 巻 1 号 p. 17-25
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    We studied epithelial regeneration from cytological, histological and microspectrophotometrical aspects. In the squamous region of uterine cervix repair cells which have characteristical atypia appears in almost 7 days after damage.
    There after they resemble normal squamous cells or metaplastic cells.
    In vaginal region their reparative processes are not different from those in the squamous region of the uterine cervix.
    Repair cells in the cervical columnar region after cryosurgery becomes most prominent and atypical in 2 weeks.
    From standpoint of clinical cytology differential diagnosis from malignant cells becomes possible by follow-up of repeat smear.
  • 松田 実, 成瀬 靖悦
    1977 年 16 巻 1 号 p. 26-31
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    The cytological presentation of nonpapillary in situ carcinoma of the urinary bladder was studied.In all 5 cases, a diagnosis of malignancy has been made by cytological examination of voided urine at a time when the patients had in situ carcinoma. The symptoms of the cases, such as pain on urination and urinary frequency, suggested chronic cystitis. There was no specific picture to be made by cytoscopy. But cancer cells were found in all 5 cases.
    The characteristic features of malignant cells in urinary sediment from the patients with carcinoma in situ were studied, and a comparison was made with the features of malignant cells from invasive carcino ma. Cytological smears of urine from the 5 patients showed little evidence of inflammation and none of necrosis, a uniform pattern of relatively small malignant cells. Most of these cells were 13-14 microns in size, round or oval in shape. Anisocytosis was rare. Nuclei were slightly enlarged and also round or oval in shape;there were always hyperchromatic nuclei with finely distributed chromatin. A few large cells and malignant cells with degenerated bizarre nuclei were found, mixed with the uniform pattern of malignant cells.These large and malignant cells were numerous in cytological smears of urine from patients with invasive carcinoma.
    Biopsy specimens appearances are sometimes misle ading and apt to be diagnosed as chronic cystitis, since nonpapillary in situ carcinoma denudes easily. Therefore, routine cytological examination of urinary sediment is most useful in the detection of carcinoma in situ of the urinary bladder.
  • 山田 喬, 陳 炯堂, 横川 正之, 福井 巖和, 久井 守, 大和田 文雄, 三谷 玄悟, 稲田 俊雄, 石渡 大介, 細田 和成, 鷲塚 ...
    1977 年 16 巻 1 号 p. 32-42
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    The cytologic morphology was studied with the patients of 12 nonpapillary, intraurothelial and microinvasive cancers and the histopathologic bases of the cancer cells were analysed. Though the histopa thologic and cellular pictures of many of intra-urothelial and microinvasive cancers simulated that of insitu cancer of uterine cervical cancer (small and uniform, hyperchromatic cancer cells), they comprised some varieties of morphology like as Bowen-like and Paget-like lesions.
  • 庵原 昭一, 武田 清一, 奥井 勝二, 佐藤 英樹, 花輪 孝雄, 増田 豁, 伊藤 健次郎
    1977 年 16 巻 1 号 p. 43-55
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    Aspiration biopsy cytology was carried out in 60 patients with thyroid diseases that were histologically 25 benign tumors and 35 malignant tumors, and in 4 controls.
    As an instrument, we used an aspiration pistol that was modified Hollender-Persson type, a disposable plastic syringe (Thermo and Niplo) and a disposable fine needle (22 Gauge). Thyroid cell clumps were obtained numerous enough to evaluate cytologically and the specimens were stained by Giemsa and Papanicolaou method.
    The microscopical findings of benign adenoma, cyst, hyperthyroidism, Hashimoto disease, papillary adenocarcinoma, follicular adenocarcinoma, medullary carcinoma, anaplastic carcinoma and liposarcoma of thyroid were described in this report.
    Intranucleic cytoplasmic inclusions were observed in 13 out of 21 patients of papillary adenocarcinoma of thyroid.
    The incidence of nuclei with the intranucleic inclusion was about1%in total cancer nuclei in each papillary cancer case.The fewer intranucleic inclusions were detected also in specimen from a patient of follicular adenocarcinoma.
    Some differences of cytological finding between papillary and follicular carcinoma were observed and described.
    Aspiration biopsy cytology could revise correctly the preoperative diagnosis exclusive of cytological examination in patients with thyroid disease.
    The accuracy of this cytodiagnosis was relatively high, the positive rate in malignant was 91.4% and the negative rate in non-malignant was 88.0%, 2 false positive cases were one patient with hyperthyroidism after isotope therapy and one patient with Hashimoto disease.
    We had no complication including bleeding, infection and cancer metastasis by our technique.
  • 小林 あや子, 宝来 威, 田村 宏, 建石 竜平, 谷口 春生
    1977 年 16 巻 1 号 p. 56-60
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    Epidermoid carcinoma is the most common malignant tumor appearing in the esophagus.There are a few previous reports on esophageal oat-cell carcinoma (small cell carcinoma), and also we have experienced two cases of the neoplasm, that produced ACTH in their tumor tissues and have ultrastructurally detectable neurosecretory granules in their tumor cells. On the results of reviewing histological samples of esophageal carcinoma resected in our hospital, there are not a few cases to be classified into small cell carcinoma besides the two cases.
    The cytological smears obtained via esophagoscopy from 7 cases of esophageal small cell carcinoma were cytologically reviewed in order to observe the cytologic characteristics of the neoplasm.The cytologic findings in the observed cases were as follows:
    (1) The arrangement of the tumor cells was irregular and showed overlappings.It sometimes showed disordered clusters, which mainly consisted of the naked-like cells.
    (2) The cytoplasm of the tumor cells was small and showed high N/C ratio.
    (3) The nuclei of the tumor cells were either darkor light, usually presenting an increased amount of finely granular chromatin and thin nuclear borders.
  • 植木 明美, 宝来 威
    1977 年 16 巻 1 号 p. 61-65
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    Several improvements have been deviced to remove the mucus of sputum and to offer a higher concentration of the cells in sputum cytology. These methods include many complicated steps and require expensive instruments. We have performed to concentrate cellular elements in sputum by means of liquefaction of mucus using acetylcysteine.
    Sputum was soaked in 50 per cent alcohol for 24 hours. The sputum was separated from the alcohol, and then was soaked in 20% acetylcysteine solution, pH 7. The mass of sputum easily dissolved within an hour. The homogenous fluid was centrifuged for ten minutes. The sediment was washed by a small amount of 50 per cent alcohol, and then it was spread on slides and allowed to dry in air. The routine Papanicolaou staining method was employed.
    The cellular elements was not morphologically altered by the mucolytic agent. The squamous epithelial cells, columnar epithelial cells and malignant tumor cells were stained without changes in cytoplasmic and nulear minutiae.Correct diagnosis by 72 per cent was made using our concentrating method on the study of 85 cases of lung cancer.
    Compared with the ordinary direct method, the improved technic reported in this paper demonstrated marked improvement by 26 per cent in the accuracy of cytological diagnosis of lung cancer.
  • 西谷 巌, 下斗米 啓介, 菊地 徳博, 一戸 喜兵衛
    1977 年 16 巻 1 号 p. 66-71
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    A relationship between changes in chromatin structure and chromosomal abnormalities has become of interest. Whereas detailed observation of nuclear chromatin in interphase was difficult in hematoxylin stained specimens by optical microscope and, for this reason, has been variously described as reticular or coagulated.
    The study demonstrates that the chromatin pattern of normal and malignant cervical cells was observed clearly as chromatin grains by the dark filled, oblique illumination method using polarized monochromatic light. Chromosomes were determined from biopsy by a direct squash technique in15patients with intrae pithelial carcinoma and 42 patients with invasive carcinoma of the cervix.
    Results reveal the following:
    1) The chromatin pattern of a normal cell is observed as a mass of grains which have various sizes and forms by this method.The grain numbers between 40 and 50 are most frequent.
    2) On the other hand, chromatin pattern of the malignant cell is observed as a tight mass of grains which have definite size and form, a pattern which seems to be similar to the undifferentiated cells.
    3) The grain numbers of the malignant cells in creased widely from 60 to 100 in most cases.
    4) In metaphase of malignant cell populations, the chromosome number range was found in two patterns; the first pattern is a clear hyperdiploid mode and other pattern, in addition, is a wide chromosomal range from 40 to excess of 100 which is not a true mode.
  • 杉森 甫, 柏村 正道, 柏村 賀子, 浜崎 康夫, 塚本 直樹, 松山 敏剛, 西村 篤乃, 滝 一郎
    1977 年 16 巻 1 号 p. 72-76
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    The cellular features of48cases with microinvasive squamous cell carcinoma of the uterine cervix were precisely examined.The microinvasive cancer was defined as microscopic cancer penetrating into the stroma no more than3mm from surface of the epithelium.
    1. Tumor diathesis was evident in 22.9% of the samples. Many of the atypical cells occurred in aggre gates. The differentiated cancer cells were found in addition to the dyskaryotic cells and undifferentiated cancer cells inducing the cellular features more variegated than those of carcinoma in situ.
    2. The cytologic findings were varied case by case. One showed cell pattern like the dysplasia, while the other showed just like the outspoken squamous cell cancer. Only 47.9% were correctly recognized as microinvasive cancer.
    3. Severity and extent of the abnormalities in surface epithelium of the cervix were well reflected upon the cellular features.
  • 越野 三男
    1977 年 16 巻 1 号 p. 77-86
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    Characteristic findings of Herpes virus (HSV) affe cted cells reported by other workers were reascerta ined in the present study. Ground glass'appearance, marked thickening of the nuclear margin, multin ucleation and its molding were frequently observed. The inclusion bodies which are said to be the most prominent features in the morphology of HSV infe ction were rather uncommon. The presence of fine granules in the nuclear vesicles was more frequently associated with acute infection and the inclusion bo dies were associated with recurrent cases. Finding on the cytoplasm served as a reference.
    In the present 23 cases which showed HSV infection cytologically, no association was found between these patients and the cervical cancer of the uterus.
    The incidence of HSV infection in the female geni talia was 0.34% of the total number of the patients screened by cytosmear and 0.14% or 1/692 of the total number of the patients visited my gynecological clinic. Although the incidence of HSV infection in the female genitalia in this report is from patients of a limited area, it was higher than those of other reports.
    The detection rate was analysed, especially related to the factors, such as age distribution, menstrual periods, seasonal fluctuation and social status.
  • 野田 定, 泊 久美子, 阿部 芳野
    1977 年 16 巻 1 号 p. 87-92
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    Serial cytologic vaginal smears were obtained from 59 pregnant women (i. e. 26 nullipara and 33 multi para) from the 36th week of gestation to the onset of labor or before rupture of the membranes, and were observed for reevaluation of hormonal cytology using Maturation Index (MI), Eosinophilc Index (EI) and Karyopiknotic Index (KPI) in late pregnancy. It was found that 22 normal pregnant women among the 26 nullipara cases gave some positive evidence of hormone chages. However 4 abnormal pregnant women in the nullipara group did not give any particular evience of hormonal changes. 27 normal pregnant women among the 33 multipara cases give rather more positive evidence.Of the 6 abnormal pregnant women in the multipara group, 4 did not give positive evidence, but two cases showed the same positive evidence as the normal pregnant women.
    The conclusion is that vaginal hormonal cytology may be useful in evaluating endocrine changes in nullipara women, but that in multipara women it may not be quite so useful.
  • 野田 定, 林田 英隆, 伏見 恵, 杉田 順子, 板東 好美, 小林 裕子, 藤原 文子, 福井 雅子
    1977 年 16 巻 1 号 p. 93-98
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    The cytological findings of adenocarcinoma and ade nosquamous carcinoma of the female genital tract in the regular collection of smears have been established and verified by many authors. However, when consi dering mailing smears we cannot apply the above criteria because the cells soon degenerate and/or have not been adequately fixed. The authors detected 22 cases of adenocarcinoma and adenosquamous carcino ma among these 42, 000 smears at the Osaka Medical Association, and had results as follows: -
    A. In adenocarcinoma of the cervix, arema rkable feature of this group was the appearance of endometrium and erythrocytes. Leucocytes appeared in every case, but histiocytes were rare. The Karyo piknotic Index tended to show a low value. Abnorma lities of nuclei were moderate, and nucleoli were not prominent. Crowding of malignant cells appeared in every case.
    B. Adenocarcinoma of the endometrium showed the same tendencies as Group A. However, leucocytes were not seen in all cases, and the Karyopiknotic Index showed a moderate value.Abnormalities of nuclei were very slight.
    C. In adenosquamous carcinoma of the cervix erythocytes appeared in all cases, distinguishing this group remarkably from others. The Karyopiknotic In dex tended to show a higher value than other groups.
    D. In metastatic adenocarcinoma of the cervix there was only one case, with a rather low Karyopi knotic Index value.
  • 特に細胞学的検討を中心として
    蔵本 博行, 上坊 敏子, 西田 正人, 鈴木 光明, 大野 英治, 鶴野 和則, 今井 忠朗, 新井 正夫
    1977 年 16 巻 1 号 p. 99-105
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    The gynecologic tumor clinic of Kitasato University Hospital has registered 1, 000 patients during three year period since founded on July 26, 1971. Statistical analyses with special reference to cytologic features were performed on these registered patients. One hundred and fourty-seven cases, 15% of the registered, were found to be malignant including 128 with carcinoma of the cervix (86.4%), six with corpus cancer and nine with metastatic cancer of extra-genital origin. CIS and stage Ia revealed 42, 32.8% of the cervical carcinomas, and 10.8% of the regitered were dysplasias. The malignancy rate of each age group showed less than 10% in 26-40 years of age and increased gradually up to more than 50% in those above 61 years.The average ages of severe dysplasia, CIS, stage Ia, and stage Ib and above were chronolo gically elevated to be 38.6, 39.8, 44.5 and 57.0 respe ctively.
    The cytologic diagnoses were made to be negative in 76.9%, suspicious in 10.6% and positive in 12.5%. There was no false negative case in cervical carcino mas. Seven cases (30.4%) of CIS, however, were diagnosed as class M a. The retrospective analysis revea-led that malignancy was ruled out in two cases, ap-parent carcinoma cells were missed in two and seve-re dysplasia rather than CIS must have been diagno sed in three. The cytologic features of the metastatic carcinomas of the stomach (3 cases), the bladder (2 cases) and the caecum (one case) were explained. False positive rate of smear tests was 0.2%.There includes a case of reparatory process which had been diagnosed as adenocarcinoma.
    Abnormal findings of colposcopy revealed 50% of squamous metaplasia, approximately 70% of each dys-plasia, 90.9% of CIS, 89.5% of stage Ia and 97.5% of stage Ib and above.Occurrences of L, F and/or G in slight, moderate and severe dysplasias, CIS and tage Ia appeared 67.6%, 68.0%, 75.0%, 78.4% and 84.2% respectively. AU was malignant in 20% when found alone and in 70% when combined with L, F or G. The malignancy rates of L, F, G and AU were 30.9%, 24.2%, 26.9% and 43.6% respectively.
    CIS and stage Ia were diagnosed correctly in 90% with either smear or colposcopy, however each cove red another in 10%, and colposcopy had its priority when diagnoses lowered the degree of dysplasias. Preoperative diagnoses using the combination of smear, colposcopy and colposcopyguided biopsy were under in one rank in 33.3% of severe dysplasias, 22.2% of CIS and 20% of stage Ia.
  • 舟木 憲一, 星合 昊, 東岩井 久, 佐藤 章, 渡辺 正昭, 森 俊彦, 星 和彦, 長池 博子
    1977 年 16 巻 1 号 p. 106-108
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    Primary carcinoma in situ of the vagina is very rare in Japan. The patient is 57 years old and it's found in screening of cervical cancer. Carcinoma in situ was located in the upper and posterior wall of the vagina. No cancer was seen in the cervix and vulva. The patient had extenden hysterectomy. It is necessary to emphasize the need for inspection of the vaginal wall and vaginal smear at the screening of cervical cancer.
  • Toshio TANAKA, Itsuko MURAKAMI, Seiji AWAI
    1977 年 16 巻 1 号 p. 109-112
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
    術前に細胞診により診断し得た骨肉腫の症例を供覧する. 患者は19歳の女性で右大腿部の疹痛を主訴として来院した. 右大腿の下三分の一において中等度の腫脹, 熱感および圧痛も認められた. レ線では右大腿骨の遠位骨端中節に禰漫性の骨透明像と骨膜の肥厚がみられた. しかし, これは必ずしも骨肉腫に特異的な所見ではなく骨髄炎の可能性も充分に持たれた. したがって術前に同局所よりの試穿刺を行った. 細胞学的にはやや小さめの単核と大きめの多核の混合した多数の細胞群がみられ, 各細胞は大体好中球の5ないし20倍の大きさを示した. 同時に多数の核分裂像も散見された. これらの細胞は一般に骨肉腫の際にみられるものであり悪性骨芽細胞と考えられた. かくして骨髄炎の可能性は全く否定しえたために第15病日に右大腿骨切断が行われ, 術後腫瘍の病理組織診により術前診断は確認された. われわれは整形外科領域においても他の分野と同じく, 細胞診の有用性, 重要性かっその容易性を強調したいと思う.
  • 1977 年 16 巻 1 号 p. 120-148
    発行日: 1977/03/31
    公開日: 2010/10/21
    ジャーナル フリー
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