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Volume 21 (1982)
- Issue 4 Pages 641-
- Issue 3 Pages 469-
- Issue 2 Pages 141-
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Volume 21, Issue 2
Displaying 1-14 of 14 articles from this issue
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Isao NAKANISHI, Hirofumi MATSUMOTO, Tsukasa FUKAE, Michiro MAEKAWA, Ta ...1982 Volume 21 Issue 2 Pages 141-146
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSCytological and histological examinations on 14 cases of early invasive epidermoid carcinoma of the uterine cervix were done and the cellular features suggestive of microinvasion were evaluated as compared with histologic findings. Most reliable cellular features of microinvasive carcinoma were the presence of syncytial arrangements of tumor cells and a mixture of malignant cells of keratinizing or intermediate type. They appeared to represent the marked proliferation of tumor cells of parabasal type and focal cellular differentiation at the sites of microinvasion respectively. Cellular detritus appeared in close association with advancing extent of stromal penetration and it seemed to be correlated with the central necrosis or severe leucocytic infiltrates in the tumor cell nests on the histologic basis. The cell pleomorphism and nuclear chromatin pattern of irregularly coarse granularity of tumor cells were subsidarily of importance for a correct diagnosis of microinvasive carcinoma.View full abstractDownload PDF (7790K) -
Ken SATO, Kaoru YOKOSUKA, Toshiyuki KUBOTA, Iwao NISHIYA, Yoshiaki ISH ...1982 Volume 21 Issue 2 Pages 147-153
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSCell samples were taken by cervical scraping from 26 cases of severe dysplasia, 33 cases of carcinoma in situ and 21 cases of microinvasive carcinoma.
Morphological features of the nucleus were examined with the Papanicolaou stained specimens. Moreover, based on the changes of nuclear size, nuclear DNA content and Hematoxylin densed substances by a microspectrophotmetric measurements in both of Feulgen stained DNA and Pap-1 labeled specimens, observation of a difference between carcinoma in situ and microinvasive carcinoma was attempted.
1) Hyperchromasia, increased nuclear-cytoplasmic ratio and anisokaryosis in morphological changes were remarkably recognized in the cells of carcinoma in situ. On the other hand, coasely densed hyperchromasia was much more marked in the cells derived from microinvasive carcinoma in contrast to carcinoma in situ.
2) In karyometric analysis, when nuclear size was enlarged beyond 13μ in Feulgen stained specimens and 12μ in Pap-1 stained specimens, such cellsas having more enlarged nucleus tended to frequently appears in microinvasive carcinoma than in carcinoma in situ, although similar patterns were obtained in the two groups.
3) It was impossible to differentiate the cells of microinvasive carcinoma from those of carcinoma in situ from the results of nuclear DNA content determined.
4) The cells of microinvasive carcinoma showed a remarkable increase of Hematoxylin densed substances in the nucleus.
5) To distinguish deffernce between carcinoma in situ and microinvasive carcinoma, it might be useful to investigate the changes of RNA and other proteins associated with DNA content in the nucleus.View full abstractDownload PDF (2976K) -
Ichiro KAI, Toshihisa NAGANO, Shiro YASUI, Yoshio TENJIN1982 Volume 21 Issue 2 Pages 154-159
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSIt has been noted in recent years that abnormal cells suggestive of malignancy occasionally appear after artificial abortion. Examination of these abnormal cells by dividing them into the decidual type, cylindrical epithelium type, and miscellaneous type, revealed that the decidual type appears mainly within 2 weeks after abortion, while most of the cylindrical epithelium type appear thereafter.
In order to elucidate the character and origin of these abnormal cells, studies have been conducted from a viewpoint of cellular nuclear DNA by performing cell analysis, using aspirated endometrial cells and re-curettage materials after abortion, in order to determine the nuclear DNA patterns with ICP, a kind of FCM. As a result, two groups, Group A having a peak in only the diploid area, and Group B having a very low peak in the triploid and tetraploid areas, were distinguishable.
Group A is observed mainly within 10 days, while most of Group B is noted after 20 days onwards. Consideration of this fact, together with the observed pattern of appearance of cells, led the author to believe that the low peak in the polyploid areas observed in Group B corresponds to newly generated cells with a brisk rate of division among the cylindrical epithelial cells. On the other hand, the abnormal decidual cells are considered to be decidual cells which have degenerated after abortion, from the fact that their DNA mode shows the normal diploid pattern.View full abstractDownload PDF (822K) -
Immuno-and Enzyme Cytochemical ObservationsMitsuaki SUZUKI, Hiroyuki KURAMOTO, Shinichi IZUMI, Keiichi WATANABE1982 Volume 21 Issue 2 Pages 160-166
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSPlacental alkaline phosphatase was localized in human chorionic gonadotropin producing gestational choriocarcinoma cell-line, designated as GCH-nu, using enzyme cytochemical and immunocytochemical techniques.
Light and electron microscopic observations revealed that the alkaline phosphatase (ALP) specific to placenta was found on the surface of microvilli of the cultured cells. With the enzyme cytochemical method, the heat stable ALP was present on the surfaces of cells which formed mound. While the cells which was spread in-between the mound were negative for the ALP. On the other hand, with the immunocytochemical method, the surfaces of all the cells including those spread between the mound were positive for placental ALP. These observations suggest that all cells possess placental ALP on their surfaces, although those on the surfaces of cells which were spread between the mound were enzymatically inactive but active as an antigen.View full abstractDownload PDF (7696K) -
Etsuo AMANO, Michio SUGITA, Tadashi SUGISHITA, Yoshio TENJIN, Norikoto ...1982 Volume 21 Issue 2 Pages 167-175
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSNuclear DNA content in utero-vaginal squamous cells was measured with emphasis on the normal exfoliated cells in order to find the characteristic DNA histograms about normal cells of different maturity and criteria for distinguishing abnormal cells, and possibilities for cytological analyses by means of impulse cytophotometer type 11 and its clinical application were explored.
A total of 72 cases, consisting of 11 cases of group A where the maturation-index on smear with Papanicolau stain showed 85% or higher leftward transition, 28 cases of group B with 85% or higher central transition, 16 case of group C with 85% or higher rightward transition, and 17 cases of group D in a few months of pregnancy, were examined and the following results were obtained.
1) Nuclear DNA content was the highest in group A followed by group B and group D in that order, and it was lowest in group C.
2) In group A, nuclear DNA content did not exceed 50 channel, while it was always below 45 channel on the X-axis of histogram in groups B, C and D. In some of group C, it was even below 35 channel.
3) In all of the groups, the ratio (3C+4C)/total did not exceed 7.8, while the ratios of 4C/2C and 4C/S never exceeded 2.2 and 3.2 respectively.
4) Existence of mitotic phase cells was suggested in groups A, B and D, although in small numbers, and further pyknotic cells were indicated in group C.
5) As to the clinical application of flow-cytometry in patients with uterine cancer, this method seems to be useful in the diagnosis of uterine cancer by determining the part exceeding 4C including (3C+4C).View full abstractDownload PDF (6165K) -
Syuitsu SAGAWA, Yoshihiro SHIINA, Yoshitaka YAMADA, Kihyoe ICHINOE1982 Volume 21 Issue 2 Pages 176-180
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSTo conserve the function of the ovary from irradiation after extensive hysterectomy in patients below 40 years with epidermoid carcinoma of the uterine cervix, the exteriorization of the ovaries with the ovarian vessels into mammary tissue has been performed.
In order to investigate clinically the endocrine function of the transplanted ovaries which show a continuous cyclic change of swelling and shrinkage for years, cytohormonal changes of the vaginal epithelium was examined.
The cyclic influence of sexual hormones on the vaginal mucosa with the regular changes in size of the transplanted ovaries was clear. Both keratinization and eosinophilic cells with pyknotic nuclei predominate at the ovarian swelling phase, but the percentage of eosinophilic cells becomes lower in the shrinkage phase.View full abstractDownload PDF (606K) -
Koji IRIE, Setsuo SUGISHIMA, Sunayo IRIE, Yasuyuki SASAGURI, Setsuro K ...1982 Volume 21 Issue 2 Pages 181-189
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSNeedle aspiration biopsy for cytodiagnosis of various lesions, especially tumors, is now routine in many hospitals. For the purpose of studying aspiration cytology of bone tumors, we examined 5 cases of giant cell tumor of bone by means of imprint cytology.
The findings of imprint smears were compared with those of histological and electromicroscopic sections. By Jaffe & Lichtenstein's grading, materials were 4 cases of grade I and one grade II.
Cytologically, characteristics of this tumor were as follows:
1) Mononuclear stromal cells were spindle or polygonal in shape and cytoplasma of them was pale lightgreen in color.
2) Nuclei of stromal cells were round or elliptical, sometimes spindle, 10-20 micron in size, averaging 13.41±1.93 micron.
3) Nuclei of stromal cells were composed of nonincreased chromatin with fine granular structure and small nucleoli were seen in 10 to 30 per cent, of them.
4) Multinuclear giant cells were polygonal in shape, 20-more than 100 micron in size and cytoplasma of them were more light-green with Papanicolaou's stain.
5) Nuclei of giant cells were of centricity, 10-more than 50 in number and the frequency of cell having more than 50 nuclei was from 6 to 46 per cent.
6) Nuclei of giant cells were round or elliptical were composed of slightly increased chromatin with fine granular pattern than stromal cells and most of them had prominent nucleoli.
At the electromicroscopic level, this tumor consisted of some types of mononuclear stromal cell and multinuclear giant cells. Stromal cells were classified into three types: undifferenciated mesenchymal cell, fibroblastic cell-most commonly encountered-and histiocytic cell. As the histological grading raised, there were decreases of fibroblastic and histiocytic stromal cells and increases of undifferentiated mesenchymal cells. From the findingsabove, however, the correlation between cytological observation and histological grading could not be established. On this point, we should farther study on many materials including giant cell tumor of bone and nonepithelial tumors composed of spindle cell cytologically.View full abstractDownload PDF (16123K) -
Tokiaki TOYOHARA, Shunji OKUBO, Masamichi OIKAWA, Kazuo OKURA, Toshimi ...1982 Volume 21 Issue 2 Pages 190-195
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSThe authors examined 100 xanthoma cells each of 12 subjects, who were diagnosed histologically to have thexanthoma of the stomach. Smears made by the squashing method were performed by Papanicolaou, Sudan III and PAS-staining and examined under oil immersion at a 1000-field magnification with a light microscope. Characteristics of cytologic findings of xanthoma cells are as follows:
1. Cells are comparatively large, and round or oval.
2. Nuclei are generally small and oval. The cells are usually mononucleated. But sometimes large bi-nucleated cells or a large polynucleated cells with three or more nuclei may appear.
3. The degree of anisokaryosis is slight, but that of anisocytosis is high.
4. The nucleo-cytoplasmic ratio is very small and the cell is rich in cytoplasm.
5. The nucleus mainly consists of a granular chromocenter with normal tinctorial properties. Chromatin is evenly distributed. The nuclear clear area is small.
6. Nucleoli are usually small and arborescent, each nucleus has one or two nucleoli.
7. The cytoplasms become filled with foamy vacuolation.
8. Reaction from the sudan III-staining is positive, but from the PAS-staining is negative.
9. The cells are generally recognized as a group of scattered cells and arranged in plane clusters, but are not arranged in clusters with threedimentional overlapping.View full abstractDownload PDF (4650K) -
Minoru MATSUDA, Akemi TAKENAKA, Sachiko NAGUMO, Yasuyoshi NARUSE1982 Volume 21 Issue 2 Pages 196-201
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSNeedle aspiration cytology was very useful in the diagnosis of skin metastases of carcinoma of internal organs.
In semears from the aspirated material, 41 out of 43 cases of skin metastases were cytologically positive. In the remaining 2 negative cases, adequate carcinoma cells could not be obtained.
Two aspirated cases of cutaneous metastases of renal carcinoma and another two of hepatocellular carcinoma which were found prior to primary lesions were reported in detail.
In the observation of smears from cutaneous metastases, it is the most important to find the characteristics of carcinoma cells in those smears and this is considered to be of help for elucidating their primary lesions.View full abstractDownload PDF (9186K) -
Michiko KIMIZUKA, Matsuo MURATA, Yasuko NOGUCHI, Tamiko TAKEMURA, Hide ...1982 Volume 21 Issue 2 Pages 202-207
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSStudy on detection of malignant cells in the routine wet-mount urine sediment was reported. In our laboratory, during 1973 and 1979, 68 malignant caces of the urinary system were diagnosed in 389, 760 urine samples by routine examination. The lesions detected include 5 cases of urinary bladder carcinoma, 1 renal pelvic carcinoma, 4 with urinary bladder invasion from uterine cervical carcinoma, 1 from prostate carcinoma, 1 from rectal carcinoma, etc. When atypical cells were found in the wet-mount specimens under the conventionalbright optical microscopic fields, the cover-glass was removed and subsequent PAP or Giemsa staining was employed for detailed cytological observation. Supravital staining using. URI-Cel appeared to be appreciable procedure. Variety of cell size, tendency to form cell clusters, irregular and stiff nuclear rim, coarse chromatinic structure, etc. were the significant features of malignant cells in the wet-mount specimens.View full abstractDownload PDF (11812K) -
in comparison with the electron microscopic findingsHideo TESHIMA, Isao INOUE, Yoshiro KIDERA, Yoshiko KASHIMURA, Masamich ...1982 Volume 21 Issue 2 Pages 208-213
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSA case of malignant melanoma observed in a married, nulliparous 61 years old woman with the complaint of genital bleeding was reported. The tumor arose in the cervix and continuously extended to the vagina forming the satellite lesion around the margin.
Histologically palmoplantar subungal mucosal melanoma was most suspected.
Cytologically most of the tumor cells were round, although spindle-shaped tumor cells were occasionally seen in groups. The cytoplasm of the tumor cells contained fine, granular, brownish pigments. On the other hand in histiocytes coarse and aggregated pigments werefound in the lysosomes. The N/C ratio was varied from low to high and eccentrically located nuclei were also observed. The tumor cells contained one or two small but distinct nucleoli with perinucleolar halo. The intranuclear vacuole that many authors had described in the literatures was observed in one tumor cell.
Electron microscopy revealed an intranuclear inclusion body of unknown origin. It was different from the cytoplasmic invagination shown by Sagebiel. The axon body-like structure was also found in the cytoplasm of the tumor cell.View full abstractDownload PDF (10177K) -
Koji IRIE, Setsuo SUGISHIMA, Sunayo IRIE, Yasuyuki SASAGURI, Minoru MO ...1982 Volume 21 Issue 2 Pages 214-219
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSA case of bronchial carcinoid diagnosed by means of bronchial scraping cytology, was studied cytologically, histologically and electron microscopically.
Cytologic findings were as follows;
1) Tumor cells were seen in single and irregular flattened clusters. A few rossete formations were found.
2) Cytoplasm was lightly stained, and cytoplasmicrim was poorly defined.
3) The nuclei of tumor cells were round 6-10 micron in size, and composed of severely increased chromatin with fine and/or rough granular pattern.
4) Nucleoli was not prominent
Histologically, the tumor was A type by Soga's classification in argyrophil type, but generally the foregutderived carcinoids were predominantly B type.
The fine structure exhibited characteristic granules of the so-called neurosecretory granules; The granules were 120-300 millimicrons in size, round and were composed of electron dense core enclosed by unit membrane. The morphological findings described above, revealed a typical case of bronchial carcinoid.View full abstractDownload PDF (10395K) -
Nobuko TANAKA, Noriko NUKADA, Yoshihiko ARAKI, Teiichi MOTOYAMA, Norik ...1982 Volume 21 Issue 2 Pages 220-227
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSRenal cell carcinoma is known to show various histo-logical features including focal or diffuse sarcomatoidpatterns in rare instances. When the specimens are obtained from the sarcomatoid lesions alone, it is very difficult to make a distinct diagnosis of renal cell carcinoma. This report deals with two cases of sarcomatoid renal cell carcinoma without any urinary abnormalities, final diagnoses of which were made at autopsy.
In these cases, the specimens obtained in life were only cytological ones from metastatic lesions with sarcomatoid variant.
One case was a female aged 48 with a chief complaint of coughing. The clinical examinations revealed abdominal tumor and multiple lung and skin tumors. The cytological specimen was obtained by aspiration biopsy of the skin tumor. The other case was a female aged 54 who complained of abdominal distention. The clinical examinations revealed abdominal tumor with a marked retention of ascites. Cells in ascites were examined.
In both of smear slides from these two cases, multinuclear or giant cells were observed solitarily and scatteredly. The nuclear membrane was not thickened.
The nuclear margin was indented, with prominent nucleoli.
From these findings the cells were mostly suspected of “sarcoma” in origin. The cell margin, however, was not so obscure as that of many kinds of sarcoma cells and small vacuoles of various degrees were found in the cytoplasm.
Electron microscopically, the tumor cells were characterized by large cytoplasm rich in cell organellae and microfibrils. Numerous and irregular cell processes were also observed but microvilli were lacking at the cell surface.
Poorly developed desmosomal junctions were infrequently seen, which strongly suggested that the tumor cells might be epithelial in nature.
The autopsies of the two cases confirmed that the tumor cells were derived from sarcomatoid lesions of renal cell carcinoma.View full abstractDownload PDF (9327K) -
1982 Volume 21 Issue 2 Pages 279-442
Published: 1982
Released on J-STAGE: January 25, 2011
JOURNAL FREE ACCESSDownload PDF (21680K)
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