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Volume 26 (1987)
- Issue 6 Pages 903-
- Issue 5 Pages 733-
- Issue 4 Pages 515-
- Issue 3 Pages 381-
- Issue 2 Pages 211-
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Volume 26, Issue 4
Displaying 1-27 of 27 articles from this issue
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With special reference to the peritoneal cytologyIkuno FUJIMOTO, Munemasa KAKU, Yasuo HIRAI, Tetsuro HAMADA, Katsuhiko ...1987 Volume 26 Issue 4 Pages 515-522
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSCell samples from the peritoneal cavity were examined in 88 patients with serous cystadenocarcinoma of ovaries, and following results were obtained.
1. The patients with positive peritoneal cytologic specimens had a poorer survival rates for 1-5 years than those patients with negative cytologic specimens. There is a statistical difference between two groups (p<0.005).
2. The incidence of histiocytes, lymphocytes, neutrophils and mesothelial cells in the background of peritoneal cytologic specimens was studied. When the incidence of these cells in patients with poor prognosis in stage I and II was compared with the incidence in patients with more than two years' survival in those stage, it was found that there were fewer histiocytes and lymphocytes and more neutrophils in patients with poor prognosis. In contrast, when the patients in stage III and stage IV who had poor prognosis were compared with the patients in stage III who survived more than two years, there were more histiocytes and lymphocytes and fewer neutrophils in patients with poor prognosis.
3. When the number of isolated malignant cells in peritoneal fluid was greater, the prognosis was poor in all stages.
4. The patients with poor prognosis tended to have more cancer cells with irregularly-shaped nucleoli and more cancer cells with macronucleoli greater than 3μ in diameter.
5. In all cases (Stage I. II. III & IV) survival rate at two years was lower for patients with positive PAS reaction than for patients with negative results.View full abstractDownload PDF (3130K) -
Comparative studies of the same cells by light and scanning electron microscopyKentoku KUMAI, Ryuichi KUDO, Hidemitsu MIZUUCHI, Hajime TAMURA, Masayo ...1987 Volume 26 Issue 4 Pages 523-531
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSIt is said that the identification of cells in ascitic fluid is sometimes difficult. In order to make a definite cytological diagnosis of cells in ascitic fluid with ovarian cancer, we observed the same cells by light and scanning electron microscopy (SEM). We also made comparative observations of the ascitic fluid cells and the surface ultrastructure of the original tissue. Specimens used were the ovarian cancer tissue and ascitic fluid cells obtained from ovarian serous cystadenocarcinoma, endometrioid carcinoma and Krukenberg's tumor patients. In addition, ascitic fluid cells from mucinous cystadenoma patients and the peritoneal tissue from myoma uteri patients were observed.
1) The same findings were obtained for the surface ultrastructure of peritoneal tissue and mesothelial cells in ascitic fluid. Mesothelial cells in ascitic fluid showed a sheetlike pattern, the cytoplasm being plate-formed with the nuclei protruding and the microvilli in a random series.
2) The surface ultrastructure of the cancer tissue in serous cystadenocarcinoma and endometrioid carcinoma, as well as of adenocarcinoma cells in ascitic fluid, showed a globular appearance with no nuclei protruding. The adenocarcinoma cells in ascitic fluid formed small spheroid clumps, the cells being densely and mutually attached by their microvilli. The microvilli on the cell surface showed multiform changes and appeared in a dense to random series. In a portion of the tissue with serous cystadenocarcinoma, cilia were found on the surface of the cancer cells. In Krukenberg's tumor, fine elongated microvilli were observed on the ascitic cancer cells and formed into a dense series. Similar findings were obtained for the cancer tissue.
3) It was observed by SEM that mesothelial cells had sparse microvilli, whereas adenocarcinoma cells had dense microvilli on the surface. The mesothelial cells were relatively flatter, whereas cancer cells formed a small grape-like structure and had a globular appearance. It was possible to distinguish between the mesothelial cells and adenocarcinoma cells by observing the cell surface under SEM.View full abstractDownload PDF (16618K) -
Keiji FUKUSHIMA1987 Volume 26 Issue 4 Pages 532-536
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThis study attempted to evaluate the hormonal status of pregnant women in the first trimester of gestation through the cytological examination of smear specimens taken from the ectocervix for cancer screening. Smears were obtained byscraping wet cotton swabs and stained by the Papanicolaou method. The author compared the karyopyknotic index (KPI), eosinophilic index (El) and navicular cell index (NI) for both vaginal smears (taken from the proximal third of the lateral vaginal wall) and ectocervical smears from 222 normal pregnant women without bleeding or infiammation, at 5-12 weeks.
According to the present examination, no significant difference in KPI and EI was found between vaginal and ectocervical specimens in 73% of cases. This frequency is slightly less than that in non-pregnant women. In the remaining 27% of the tested cases, the indices (especially EI) were generally higher for the ectocervical specimens than for the vaginal specimens, but they scarcely exceeded the value of 50, which indicates danger of abortion.
The examination showed no significant difference between vaginal and ectocervical specimens in terms of NI.View full abstractDownload PDF (676K) -
Sonoko NATSUME, Masato SHINFUKU, Ritsuko IMAI, Mie HIRANO, Tatsunari S ...1987 Volume 26 Issue 4 Pages 537-541
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTwelve cases with metastatic uterine tumors were examined cyto-histologically. The primary sites were the stomach, colon, lung and ovary, respectively. Metastatic tumor cells were seen in the cervical smears in all cases.
In the majority of cases, tumor cells were relatively few in number, appearing singly or forming small clusters in the smears. Stomach cancers were strongly suspected when adenocarcinoma cells were present as signet ring cells.
Tumor cells seemed to spread in the uterine wall and reached the uterine surface by way of lymphatics except by direct invasion, in our precisely histologically examined five cases. In these cases, metastatic tumors did not localize only in the cervix but in more than one of the vaginal wall, uterine cervix, corpus and fallopian tubes.
Five of twelve cases did not complain of gynecological symptoms such as vaginal bleeding. Thus, it was thought necessary for female cancer patients to be examined gynecologically, especially including cervical smears.View full abstractDownload PDF (5040K) -
Kunio WAKITA, Norimitsu SASAKI, Hiroyuki KURAMOTO1987 Volume 26 Issue 4 Pages 542-550
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTwo hundred and thirty-two patients with various diseases of the cervix (dysplasia 135, CIS 30 and portio erosion 67) were treated with laser vaporization, and the healing process was followed by cytology and colposcopy.
1) Sixty-five patients with YAG laser vaporization followed by cytology revealed fiber like cells in 50.8% by the second week and especially in 70.0% immediately after vaporization. Repair cells were found later in incidence of 29.2% and metaplastic cells in 30.8%. The epithelization began from the side of squamous epithelium at 2nd week and the wound healed forming a new SCJ observed through colposcopy.
2) The cytologic smear after vaporization showed abnormality in 19.3% of 135 cases with dyaplasia, 16.7% of 30 with CIS and 7.5% of 67 with portio erosion. On the other hand, colposcopic abnormality showed in 19.3%, 13.3% and 3.0% respectively.
3) Among 36 patients who developed abnormality in cytology, 83.3% were observed by the 10th week and others between the 11th and 44th. In case of portio erosion, they were all seen by the 5th week. Colposcopic abnormality was also observed in 61.5% of dysplasias and 80.0% of CIS who showed abnormal cytology. Eleven cases were confirmed to have residual neoplasia by biopsy.
4) In spite of normal cytology, colposcopic findings revealed abnormal only in 7.0% of dysplasia and 3.0% of portio erosion.
5) Analyzing patients who sustained residual lesions it is plausible that cytology during 5-6th and 10-11th weeks has a key role for early detection of the residue, and also suggested that this has some relation to koilocytosis.View full abstractDownload PDF (10240K) -
Keiji KERA, Fumio HORIUCHI, Bin TAKEDA, Hiroyoshi TAKAMIZAWA1987 Volume 26 Issue 4 Pages 551-558
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSSix biopsies of the uterine cervix which were diagnosed clinically as flat condyloma and histologically proved to be koilocytotic atypia were ultrastructurally studied.
(1) In all six cases, cells with a clear perinuclear area with sparse organella were observed in some of the intermediate and superficial epithelial layers, associated with varieties of changes involved in clear zone formation, such as accumulation and fusing of small clear areas in the perinuclear zone.
(2) The author detected electron-microscopic findings consistent with such light-microscopic features as karyomegaly, bi-or multinucleation, hyperchromasia of nuclei and dyskeratosis.
(3) In one case, HPV particles were found in superficial cells with nuclear enlargement, some atypia and increased chromatin. Numbers of HPV particles in those nuclei differed from cell to cell.
(4) HPV particles were about 40 to 50nm in diameter and round in shape. They were observed mostly adherent to heterochromatin areas and were scattered or partly gathered in the nuclei. They were closely associated with the reticular network of intranuclear filaments.
(5) Rarely present were cylindrical HPV particles, from 20nm in width and 100 to 150nm in length, and mixed with round particles, in irregular or crystalline arrangement.View full abstractDownload PDF (13147K) -
Norio CHIWATA, Tadashi SUGISHITA, Norikoto ISHIDA, Yataro YANAGIZAWA, ...1987 Volume 26 Issue 4 Pages 559-565
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSIn the present study, cytological examination was performed in 9 cases of cervical human papillomavirus (HPV) infection with mild dysplasia during the follow-up period.
1) Similar to the case of cervical HPV infection with no dysplasia, koilocytes repeatedly appeared and disappeared in most cases of cervical HPV infection with mild dysplasia.
2) In the case of associated mild dysplasia, nuclear atypism appeared frequently on some identifiable findings which were observed by cytological examination during the follow-up course.
3) In 6 of the 9 cases, whole or a part of cytological findings so far observed was found to be emphasized at the onset of mild dysplasia.
4) In most cases (8/9), nuclear atypism appeared mainly in small cells of immature metaplastic type or accompanying with dyskaryotic cells of superficial type or intermediate type.View full abstractDownload PDF (7341K) -
Sonoe ITO, Yoshitaka OHTA, Akiko HARATAKE, Yuko MORITUKA, Masato MARUY ...1987 Volume 26 Issue 4 Pages 566-571
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe examined eight human cases of polyomavirus infection using the following cytological and ultrastructural studies. Patients' urine contained intranuclear inclusion-bearing (INIB) cells at rates of more than 90% in children and about 30% in adults.
Under Papanicolaou staining, INIB cells were slightly light greenish in color. Nuclei of INIB cells were either pseudocircular or round and were 11 to 18μm in diameter. INIB cells showed an increased N/C ratio. Most nuclei contained inclusions showing a marked ground-glass apearance. In all eight cases, both bird's eye shape and karyorrhexis were occasionally observed. Multinucleated superficial transitional cells were found in four cases. These nuclei also presented a ground-glass appearance. Intracytoplasmic inclusion cells often caused by virus infection were also observed in all cases.
Under electron microscopic examination, nuclei of INIB cells contained virus particles which appeared to be human polyomavirus. These virus particles, which lacked their envelope, aggregated in a crystal arrangement and their diameter ranged from 30 to 40 nm. Tonofilaments found in the cytoplasm of INIB cells indicated that these cells were transitional in origin.View full abstractDownload PDF (7480K) -
Charac teristics, function and ontogenyYutaka IMAI, Mitsunori YAMAKAWA, Kunihiko MAEDA, Akihiro MASUDA, Toshi ...1987 Volume 26 Issue 4 Pages 572-580
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe studied the characteristics, function and ontogeny of milky spots and macrophages in the omentum and peritoneal cavity, using human and experimental animal materials.
Immunocytochemically, macrophages were stained by monocyte/macrophage markers, i.e., ferritin, lysozyme, alpha-1-antitrypsin, alpha-1-antichymotrypsin, Leu M2 and M5, whereas mesothelial cells were not stained by these markers. As a result, it may possible to distinguish macrophages and mesothelial cells by immunocytochemical methods.
Human and rat omental milky spots revealed a variety of cell types: macrophages and lymphocytes, plasma cells, eosinophils, mast cells, fibroblasts and blood capillaries. Electron microscopically it was observed that milky spot macrophages passed through the mesothelial stomata and infiltrated the peritoneal cavity. Therefore, it might be suggested that milky spots are a source of peritoneal macrophages. From the results of studies concerning macrophage ontogeny using rat and avian experimental models, it could be concluded that milky spots had two macrophage compartments which were derived from blood-borne monocytes and intraembryonic tissue, apart from yolk-sac-derived cells.
A variety of foreign substances and stimulants were administrated intraperitoneally and observed over time. Many foreign substances were found to accumulate in omental milky spots. Furthermore, lymph follicles were seen in the omentum, including milky spots. It was concluded that milky spots played important roles in the mechanism of foreign body removal and in immunological reactions in the omentum and peritoneal cavity.View full abstractDownload PDF (12457K) -
With special reference to the CA 125Hitoshi ITO, Reiko SHINODA, Yuko AKATSUKA, Kenichi KAKUDO, Yoshiyuki O ...1987 Volume 26 Issue 4 Pages 581-584
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSIn order to clarify immunohistochemical characteristics of mesothelial cells from body fluid, several antigens including tumor markers (CEA, CA 19-9, CA 125 and Alpha-Fetoprotein), cytoskeletal proteins (keratin, vimentin, desmin), S-100 protein and EMA were studied on cytological smears as well astissue sections using indirect immunoperoxidase technique. The mesothelial cells were positive for keratin and vimentin in the cytoplasm intensively. Further, significant numbers of them proved to be positive for CA 125, both in the cytological smears and tissue sections. It was localized either on the cell membrane or diffusely in the cytoplasm. It should be noted that some of the mesothelial cells are immunoreactive for the tumor marker, when immunocytochemistry is applied to differentiate malignant cells from reactive mesothelial cells in the body fluid.View full abstractDownload PDF (5584K) -
Shinji KAKUMU, Koji KATSUTA, Hideki TAKANARI1987 Volume 26 Issue 4 Pages 585-592
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBoth gastric biopsy and gastric brushing of the lesion under direct vision endoscopy were done in 447 patients with various gastric diseases. A gastric carcinoma was confirmed in 158 cases by resected materials (118 cases) or biopsies (40 cases); 58 cases of early stage and 100 cases of advanced stage. Gastric biopsy was positive in 136 cases (86.1%), suspicious in 9 cases (5.7%) and negative in 13 cases (8.2%) while gastric brushing was positive in 134 cases (84.8%), suspicious in 13 cases (8.2%) and negative in 11 cases (7.0%). Either biopsy or brushing, or, both was positive in 155 cases (98.1%).
The reasons that either biopsy or brushing could not detect a carcinoma were retrospectively analysed in 43 cases. Biopsy materials did not contain cancer cells because of dimpling microcarcinoma or contained only degenerative tissue because of necrosis of the surface of advanced carcinoma. In smears from gastric brushing, cellular atypism was very mild in well differentiated adenocarcinoma or the smears contained far few cells.
Cytology of gastric brushing was suspicious positive in 49cases of 447 patients. They included 13 cases of carcinoma, 15cases of chronic gastritis, 9 cases of gastric ulcer, 11 cases of atypical epithelial hyperplasia (ATP) and a case of reactive lymphoid hyperplasia.
Three cases in which it was difficult to diagnose in smear obtained by gastric brushing were represented: 2 cases of moderately or well differetiated adenocarcinoma and a case of chronic gastritis with benign atypical cells.View full abstractDownload PDF (6229K) -
Hiroshi MIYAMOTO, Yoshikazu ARAYA, Masami ITOH, Hiroshi ISOBE, Hirotos ...1987 Volume 26 Issue 4 Pages 593-598
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSSquamous cell bronchial carcinoma cells obtained from 28 patients who had p. stage Ia cancer excluding early lung cancer within the bronchial wall by bronchial brushing were examined to determine whether the cytomorphologic features could be related to prognosis. The five-year survival rate was 58.3% in 12 cases of central origin and 91.7% in 13 cases of peripheral origin (p<0.05). In cells from central tumors with a poor prognosis, nuclear size was 10.2±1.7μm (mean±S.D.) in diameter. In cells from peripheral tumors with a good prognosis, the nuclear size was 14.4±1.2μm (P<0.001). Four out of six patients whose nuclear size was small (<11 μm) died within 18-27 months.
These results suggest that the grade of malignancy of squamous cell carcinoma of the lung and the prognosis of patients may be determined by study of the distribution of nuclear type in the tumor.View full abstractDownload PDF (4878K) -
Toshiro KAWAI, Hiroshi MIURA, Naohisa TSUNODA, Sachiko KUBONO, Eriko H ...1987 Volume 26 Issue 4 Pages 599-606
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThe fine needle aspiration and imprint cytologies obtained from 13 cases of resected thymoma were studied. The results were as follows;
1) The fine needle aspiration cytology permitted accurate diagnosis in 6 cases of 7.
2) Cytology of thymoma was characterized by two cell pattern composed of epithelial cells and lymphocytes.
3) Cytologic features were divided into four: lymphocytic predominant type, mixed type, clustered-epithelial type and spindle cell type.
4) All three cases of clustered-epithelial cell type in cytology revealed the malignant findings such as invasion and metastasis by histologic study.
5) The nuclear size of epithelial cell had a tendency to corelation to degree of malignancy. But there was no differ-ence of the nuclear size between the metastatic case of the clustered-epithelial type and the non-invasive case of the mixed type.View full abstractDownload PDF (14268K) -
Toshiro KAWAI, Hiroshi MIURA, Naohisa TSUNODA, Sachiko KUBONO, Eriko H ...1987 Volume 26 Issue 4 Pages 607-613
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSCytologic and histologic findings in six cases of mediastinal benign cystic teratoma were reported.
All six cases were accurately diagnosed by fine needle aspiration or pleural effusion cytology. It was diagnostically important to observe the macroscopical yellowish-brown oily material obtained from the cyst.
The aspirate was small in amount but cytologic findings were characterized by squamous cell, keratinous material, anuclear or multinuclear foamy macrophage, crystalline structure and necrotic tissue.
Histologically, the main cyst was lined by a stratified squamous epithelium with skin adnexae. The cyst content was composed of keratinous material. Foamy macrophages and foreign-body giant cell reaction were seen. Pancreatic tissue was found in three cases out of six.
Mediastinal teratoma was characterized by the tendency to rupture compared with teratoma arising in other sites. In case 1, the tumor ruptured into the lung and bronchial washing cytology revealed inflammatory cells, necrotic tissue and keratinized squamous cells. In case 4 and 6, pleural rupture of mediastinal teratoma was diagnosed by the presence of cyst content including hair in the pleural effusion.View full abstractDownload PDF (11204K) -
Sakae HATA, Setsuko OOTA, Chotatsu TSUKAYAMA, Sadaaki NAKAGAWA1987 Volume 26 Issue 4 Pages 614-620
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSAmyloidosis is a rare but well-known disease, which occurs spontaneously or secondarily to some chronic diseases. In addition to these primary and secondary type, both localized and familiar form are recognized as well. Although amyloidosis discloses variable clinical signs it is rather rare that this lesion may be diagnosed clinically.
Recent aspiration cytology technique made it easier to detect the amyloid substance in the suspected cases as well as in the incidental cases of amyloidosis.
Recently we have had the chance to observe amyloid substance with cytological examination in 5 cases, including 3 systemic type and the other two localized form: medullary carcinoma of the thyroid.
Amyloid was found to be compound of amorphous substance, ranging from 5 to 1, 000μ in diameter, associated occasionally with a few lymphocytes and plasma cells, and stained green to dark blue with EA65.View full abstractDownload PDF (11892K) -
Shigetaka MIZUNO, Hiroshi ISOBE, Masami ITO, Shoichi INOUE, Hiroshi MI ...1987 Volume 26 Issue 4 Pages 621-627
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSPepsin enzymatic disaggregation of lung carcinoma tissues for flow cytometric DNA measurement was studied. Cell suspensions were obtained from human primary lung carcinomas obtained at surgery or autopsy. Mechanical and enzymatic disaggregation procedures were used. For mechanical disaggregation, tumor tissue was sliced to 5mm in thickness and minced. The cells were dispersed by forced filtration through a metal mesh. For enzymatic disaggregation, cell suspensions taken after the mechanical procedure were incubated in various concentrations of pepsin for various periods at room temperature or 37°C. Optimum conditions for the pepsin disaggregation method were studied by measurement of changes in CV and peak channel number for G0/G1 peaks by flow cytometric analysis. In this study, optimum conditions for the disaggregation of squamous cell carcinoma and adenocarcinoma were incubation in solutions containing 0.1 to 0.5% pepsin for 10 to 30 minutes at 37°C, and for small cell carcinoma, 0.05 to 0.25% pepsin for 10 to 15 minutes at 37°C.View full abstractDownload PDF (5435K) -
Yasuhiro KATSUBE, Makoto YORISHIMA, Yasuhisa IWAOKI, Tsuneo FUJII, Ken ...1987 Volume 26 Issue 4 Pages 628-633
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThe prognosis of dysgerminoma ovarii varies with different reports. Two cases of pure dysgerminoma which showed different clinical aspects are reported, with a comparison of the cytologic and histopathological features of the two cases. The first case is alive with no evidence of disease five years after operation and chemotherapy. The other case showed excellent response to radiotherapy for recurrent disease after initial postoperative chemotherapy, but died of fuluminant hepatitis.
Cytologic features in these two cases were as follows. The large tumor cells had scant cyanophilic cytoplasm and showed a high N/C ratio. In both cases, the unclei contained coarse chromatin and one or a few prominent uncleoli, but the nuclear atypia and pleomorphism, and the irregurality of the perinuclear membrane were more apparent in the second case. With regard to histopathological findings, the first case was characterized by a trabecular or insular arrangement and marked lymphocytic infiltration. The second case was characterized by a diffuse pattern, with numerous mitotic figures and extensive necrosis.View full abstractDownload PDF (11894K) -
Toshihiko TOKI, Yuya ABE, Yuichi WADA, Akira YAJIMA, Shuku SUGAWARA, F ...1987 Volume 26 Issue 4 Pages 634-638
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of primary adenocarcinoma of the vagina probably arising from endometriosis is reported. The patient was a 38 year-old woman. An exophytic, nodular and little fingersized tumor was found in the posterior fornix of her vagina. Malignant cells suggesting adenocarcinoma appeared in a preoperative imprint smear of the tumor. The condition was diagnosed histologically as poorly-differentiated adenocarcinoma. Not only foci of benign endometriosis but borderline malignant lesions occurred in the rectovaginal septum beneath the carcinoma. Apparently this neoplasm arose from endometriosis of the rectovaginal septum.View full abstractDownload PDF (7651K) -
Kaoru AKURA, Mitsue HATAKENAKA, Masaharu TAKENAKA1987 Volume 26 Issue 4 Pages 639-643
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSHuman polyomavirus-infected cells were detected in the urine of an insulin-dependent diabetes mellitus patient. Sixteen cytological specimens were obtained and examined in the two-year period from June 1984 to June 1986. All the specimens examined with Papanicolaou staining contained a few decoy cells in which the unclei had homogeneous and basophilic inclusion bodies and heavy chromatin deposits on the inner margin of nuclear membranes.
These intranuclear inclusion bodies were positive for Feulgen reaction, suggestive of DNA-enriched material. The cytoplasm was scanty and degenerated.
Electron microscopic study revealed that the uncleus was packed with freely scattered small virus particles (apporoximately 40nm in diameter). After destaining Papanicolaou preparations, immunocytochemical study was carried out using a polyclonal antibody against sodium dodecyl sulfate (SDS) disrupted SV40 virion (SV40VP1). The apparently positive cells were localized in the nuclei of the inclusion body-laden cells. The correlation of polyomavirus infection with diabetes mellitus is discussed.View full abstractDownload PDF (6167K) -
Significance of cytologyShunichi SASOU, Tamotsu SUGAI, Kazuo TAKAYAMA, Ayako WATANABE, Hiroshi ...1987 Volume 26 Issue 4 Pages 644-646
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of urachal carcinoma is presented. The patient was a 40-year-old man who was admitted with the chief complaints of pain on micturition, hematuria and mucin contamination in the urine. Upon cystoscopic examination, a tumor was found in the dome. A cytological examination, using the irrigation collection method, showed a mucin-producing adenocarcinoma. The tumor was situated in the wall of the bladder and was histologically diagnosed as mucinous adenocarcinoma with a papillary adenocarcinoma lesion at the surface of the bladder.
Cytological examination of bladder washings may be of diagnostic value in identifying urachal tumor.View full abstractDownload PDF (3849K) -
Takayuki NOJIMA, Kazuaki INOUE, Miri FUJITA, Syuichi HOUKIN, Mikio ARA ...1987 Volume 26 Issue 4 Pages 647-651
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA rare case of solid and cystic tumor of the pancreas in a 10-year-old girl is reported. The encapsulated tumor is 5cm in diameter and has necrosis and a slight hemorrhage. Cytological examination of imprint samples shows that tumor cells have regular homogenous cytoplasm, round to oval nuclei and smooth and thin nuclear border. Most of tumor cells are positive for apantitrypsin and neuron-specific enolase. The tumor has papillary and cystic patterns with regular eosinophilic cells and very rare mitosis, and in some areas tumor cells show irregular pseudorosette formation arranged aroud small capillaries. Ultrastructural studies show numerous mitochondria, sparse endoplasmic reticulum and a few zymogen-like granules. The detection of ai-antitrypsin and zymogen-like granules suggests that acinus is the origin of the tumor.View full abstractDownload PDF (10669K) -
Shinji KAKUMU, Koji KATSUTA, Masatoshi NODA, Hideki TAKANARI1987 Volume 26 Issue 4 Pages 652-658
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThis report repsented a case of rectal carcinoid diagnosed by brushcytology obtained by direct-vision. A 26-year-old man had a polypoid lesion of the rectum. A rectal biopsy showed poorly differentiated adenocarcinoma. However, a smear from rectal brushing contained tumor cells which were identical with those of imprint smear of carcinoid tumors described in the literatures. Based on the brushcytology, polypectomy was done. The removed polyp showed histologically submucosal carcinoid with invasion to mucosa. The silver-staining of tumor cells was negative and the electron microscopic study of tumor cells showed neurosecretory granules of hidgut carcinoids.View full abstractDownload PDF (13730K) -
Pulmonary lymphangitis carcinomatosa due to gastric adenosquamous carcinoma, with cytologic findingsA case reportYoshinobu OHSAKI, Eiichi SAKAI, Yasuhito HONDA, Yuji IKEDA, Masami SAS ...1987 Volume 26 Issue 4 Pages 659-663
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA 35-year-old man with dry cough and mild fever was referred to us. Physical examination revealed tachycardia of 109/min. and an increment of bronchial sound over the whole lung. No lymph node swelling was palpable. His liver function was slightly impaired. The CRP was 2+, and the CEA was 42.0ng/ml (EIA). A chest roentogenogram showed a fine nodular and infiltrative shadow spreading from the bilateral hila to the lung fields. There was cardiomegaly and bilateral pleuraleffusion. Bronchofiberscopy showed a red, edematous change of the bronchial mucous membrane with small nodules on it. Sputum cytology revealed malignant cells with mucous which were stained by orange-G and light-green. Malignant cells arranged in a layer-like pattern, were obtained from the bronchial wall by brushing. There was a PAS positive substance in the cytoplasm. The postmortem diagnosis was adenosquamous carcinoma of the stomach, lymphangitis carcinomatosa, pleuritis and pericarditis carcinomatosa. Epidermoid transition from adenocarcinoma was observed histopathologically in the same lesion. The variety of cytologic findings of this pulmonary lymphangitis carcinomatosa due to gastric adenosquamous carcinoma are presented.View full abstractDownload PDF (6458K) -
Yoshio ODA, Ei KAWAHARA, Isao NAKANISHI, Haruki NAKAMURA1987 Volume 26 Issue 4 Pages 664-669
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA Leydig cell tumor arising in the testis of a 35-year-old male with Klinefelter's syndrome is reported. The patient, whose karyotype was 46, XX/47, XXY complained of infertility. His testes were bilaterally small, and no germinal cells were found in the atrophic seminiferous tubules, which were lined only with Sertoli cells. A yellowish brown Leydig cell tumor, measuring 0.9×0.9×0.6cm in size, was located in the upper portion of the left testis. The imprint smear obtained from a cut surface of the tumor showed that the tumor cell had a round to oval, eccentric nucleus with one or two prominent nucleoli, and abundant, pale, cyanophilic cytoplasm with a large number of vacuoles, which, on the basis of electron microscopic study, were very likely derived from well-developed smooth endoplasmic reticulum. Some tumor cells had abundant lipofuscin. Reinke's crystals were found in and out of the tumor cells. There were neither areas of tumor cell necrosis nor marked mitotic activity suggestive of malignancy in the imprint smear.View full abstractDownload PDF (10558K) -
Kimio HASHIMOTO, Yukari ISHIDA, Hitoshi MIMURA1987 Volume 26 Issue 4 Pages 670-673
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThe cytological features of a medullomyoblastoma arising in the fourth ventricle of a 34-month-old boy are described.
Tumor cells had oval and indented nuclei with coarse chromatin and indistinct nucleoli. These cells were partially arranged in a Homer-Wright rosette. Cells with large cytoplasm had clear cross-striation on May-Giemsa staining and were identified as rhabdomyoblasts. These two cell components in the same tumor suggested that it was a medullomyoblastoma.View full abstractDownload PDF (8663K) -
A case reportHiroko KOTANI, Yuuko KOORITANI, Katsutoshi MIHARA, Seiichi SUGIHARA, M ...1987 Volume 26 Issue 4 Pages 674-677
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSInfectious mononucleosis is a benign proliferative disease of the lymph nodes. We recently had the opportunity to observe the cellular features of the cervical lymph node of 21-year-old woman by aspiration cytology.
The cytologic pattern of aspirated materials from the cervical lymph node was pleomorphic, consisting of a large number of lymphocytes, with some lymphoblasts and prolymphocytes, in addition to the characteristic atypical lymphocytes, highly suggestive of a reactive pattern to infection.
The atypical lymphocytes were mixed in with various kinds of lymphocytes and were found in 12.6% of the cells.
The atypical lymphocytes observed in aspirated smears from the cervical lymph node were similar to those of atypical lymphocytes in the peripheral blood.View full abstractDownload PDF (5246K) -
A case of pleural effusion caused by chronic pancreatitis showing severe atypia of mesothelial cellsAkihiro KANEOKA, Tomiko KUROKI, Matsuo KINUGASA, Shuichi MIYAKE, Tamia ...1987 Volume 26 Issue 4 Pages 678-682
Published: July 22, 1987
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSPleural effusion is a rare complication of pancreatitis. We report a case of pancreatic pleural effusion with large numbers of atypical mesothelial cells, indicative of malignancy.
A 54-year-old male had a long history of heavy drinking and complained of coughing, bloody sputa and left chest pain. Chest X-ray revealed massive effusion in the left pleural cavity. Cytological examination of the fluid showed many atypical cells, partly scattered and partly grouped. They consisted of large, ovoid, mononuclear or multinuclear cells, as well as small, round mononuclear cells with a high nuclearcytoplasmic ratio. Hyperchromatic nuclei with distinct nucleoli were located either centrally or peripherally in the swollen cytoplasm. Numerous bleb-like surface structures were observed at the cytoplasmic margin. Based on these cytological findings, we suspected that the patient had malignant mesothelioma.
However, the results of topographical examinations such as chest X-ray, CT scan and 67Ga-scintigraphy were not consistent with the findings of malignant mesothelioma. The amylase levels of the pleural effusion, serum and urine were markedly elevated. Endoscopic retrograde pancreatography disclosed the internal fistula between the pancreatic duct and the mediastinum, lead ing to a diagnosis of chronic pancreatitis with pleural effusion of pancreatic juice through the fistula.
The patient was given a fat-free diet and underwent continuous pleural aspiration. The cytological atypia of mesothelial cells in the fluid gradually decreased. The appearance of atypical mesothelial cells in the pleural fluids might have been a reactive change to pancreatic juice.View full abstractDownload PDF (8444K)
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