All issues
Volume 28 (1989)
- Issue 6 Pages 739-
- Issue 5 Pages 603-
- Issue 4 Pages 477-
- Issue 3 Pages 327-
- Issue 2 Pages 167-
- Issue 1 Pages 1-
Volume 28, Issue 3
Displaying 1-24 of 24 articles from this issue
- |<
- <
- 1
- >
- >|
-
Yukinori YONEMOTO, Yuichi WADA, Ichiro NASU, Setsuko TAKEDA, Susumu SU ...1989 Volume 28 Issue 3 Pages 327-331
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSNineteen women with cervical adenocarcinoma were studied in order to investigate the cytological features of cervical adenocarcinoma that was suspicious in cytodiagnosis. The cytological specimens of patients were compared to those of normal women. Cytological findings in the cytologically suspicious cases of cervical adenocarcinoma were as follows.
1. The arrangement of atypical cells was “side by side” and the amount of chromatin was increased.
2. In the specimens of the one group of the suspicious cases were observed atypical cells whose nucleuses were more enlarged than those of normal cells. Their cellular atypism was nuclear enlargement and anisokaryosis and nuclear pleomorphism, but this atypism was not so striking as the atypism of the cytologically positive cases of cervical adenocarcinoma.
3. In the specimens of the other group of the suspicious cases were observed atypical cells whose nucleuses were the same size as those of normal cells. Their cellular atypism was anisokaryosis and nuclear pleomorphism and elongated form of nucleus.View full abstractDownload PDF (3747K) -
Masaaki Suzuki, Yoshinori Furugen, Hiroshi Utsuno, Michio Takada1989 Volume 28 Issue 3 Pages 332-340
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTwenty-five women suffering from cervical human papillomavirus (HPV) infection with vulval condylomata acuminata were followed up by cytological examination for over one year to establish the biological potential of HPV.
Seventy-six %(18/25) of the patients were between 10 and 20 years old. In the nonpregnant group, the incidence of koilocytosis on the cytological examination was 33% the first time, 16% after 3 month, 11% after 6 month, and 11% after 1 year. However, the respective incidences including dyskeratosis and binucleation with koilocytosis were 77%, 71%, 61% and 22%. Furthermore the results of follow up by colposcopy and histologic examination after 1 year were as follows: 6% slightly progressed, 33% persistent, 39% regressed and 22% disappeared. Condylomatous cells did not always appear during follow up cytologic examinations except in the patients with immunosuppressive condition, but colposcopic findings were usually observed. In the pregnant group (7 cases), cases with slight progression accounted for 39% of the total.
The results suggested that the cervical lesions estimated as HPV 6/11 tended to regress and disappear after 1 to 1.5 years. However, HPV 16 was sometimes associated with cervical lesions of HPV infection. Therefore, persistent cases should be followed up over the long term by HPV DNA typing.View full abstractDownload PDF (8825K) -
Kazuyoshi HIGUCHI, Makoto KAWAI, Hiromitsu YABUSHITA, Masayoshi NOGUCH ...1989 Volume 28 Issue 3 Pages 341-345
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe detected cases of HPV-DNA positive in cervical dysplasia and cervical cancer by using the RNA probe on human papilloma virus (HPV).
1) Cases diagnosed by cytological analysis that were also HPV-DNA positive: There were 2 (50%) of 4 cases in class Ina, 2 (50%) of 4 cases in class Bib, 1 (50%) of 2 cases in class IV, and 2 (33%) of 6 cases in class V. Therefore 7 (44%) of 16 cases in a class more advanced than class Ma, were positive.
2) Cases diagnosed by pathological analysis that were also HPV-DNA positive: There was 1 (100%) case of mild dysplasia, 3 (43%) of 7 cases of CIS, and 2 (29%) of 7 cases of SCC.
3) 3 (43%) of 7 cases of HPV-DNA positive displayed koilocytes.
4) 85 (99%) of 86 cases detected by mass-screening of uterine cancer were HPV-DNA negative, and were of class I and class II. Only one case was HPV-DNA positive, and was proved to be in class IIIa.View full abstractDownload PDF (2290K) -
Hideo TESHIMA, Noriyuki FURUTA, Hiroaki YAGI, Jui-Tung CHEN, Kazutake ...1989 Volume 28 Issue 3 Pages 346-351
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSCorrelation between CIN (cervical intraepithelial neoplasia) and HPV (human papilloma virus) infection was studied by cytology, histopathology and filter DNA hybridization.
1) HPV genome was detected in 66.7%(6/9) of condylomata acuminata, in 30.8%(4/13) of mild dysplasia, in 75%(3/4) of moderate dysplasia, in 50%(2/4) of severe dysplasia and 80%(8/10) in carcinoma in situ, while in 2%(1/50) of normal women.
2) When HPV genome was negative by filter DNA hybridization, there were cytological and histopathological evidences of HPV infection in 44%(4/9) of mild dysplasia and in 50%(1/2) of severe dysplasia. This suggests the possibility of uncertain type of HPV infection.
3) When HPV genome was positive by filter DN hybridization, histopathological evidences of HPV infection were found in mild, moderate and severe dysplasia but was not found in carcinoma in situ.View full abstractDownload PDF (5245K) -
Shinichi IGARASHI, Masahiro MAKI1989 Volume 28 Issue 3 Pages 352-355
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe studied clinical and cytological significance of NCC-ST-439 in gynecology. It was a new monoclonal antibody which recognized tumor associated antigen. We examined the location of its corresponding antigen in tumor and normal cells using immunocytochemical method.
NCC-ST-439 antigen showed positive in the cases of cervical carcinoma (3/11 cases, 27%) endometrial carcinoma (6/11 cases, 55%) and ovarian carcinoma (6/11 cases, 55%). But it was negative in cells of normal cervical epithelium, endometrial gland, villi and decidua.
It was suggested that NCC-ST-439 was interesting and useful tumor marker, which had low false positive ratio, in gynecological cytology.View full abstractDownload PDF (6392K) -
Keiji KANMA, Yasuki SAITO, Tadashi IMAI, Satomi TAKAHASI, Katsuo USUDA ...1989 Volume 28 Issue 3 Pages 356-361
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe studied 14 suspected cases of lung cancer, which were evaluated as positive by cytologic screening in the Miyagi Program from 1982 to 1984. None of these demonstrated any positive findings upon chest x-ray at the initial screening. The sputum specimens obtained from these cases at initial screening were reevaluated ; three of these were determined as positive, ten as suspicious, and one as negative. Twelve patients were followed up with further sputum cytology, and two patients rejected reexamination. One case was diagnosed as carcinoma in situ after bronchial brushing, but no abnormal findings were detected upon reexamination. We also found abnormal cells caused by megaloblastic anemia in one case. One patient who had rejected the reexamination died of lung cancer two and a half years after the initial screening. We continued the follow-up programs in eight cases. One year after, one of these was revealed to have developed a lung cancer which was diagnosed as a peripherally-located occult cancer. Ten out of the fourteen cases have not shown any evidence of lung cancer for more than two years.View full abstractDownload PDF (6863K) -
its comparison with electron microscopic findingsTadashi IMAI, Yasuki SAITO, Noriyoshi NAGAMOTO, Katuo USUDA, Shinichir ...1989 Volume 28 Issue 3 Pages 362-371
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBrushing cytologic findings of seventeen cases of resected early squamous cell carcinoma of the bronchus were studied cytomorphometorically, and compared with light and electron microscopic findings.
With the increase of nuclear area, nuclear/cytoplasmic ratio and cellular atypia of the cancer cells in the upper and lower layer, there were greater tendency to invasion and increase of the cancer cells, where cytoplasmic processes and intercellular spaces were less well developed and the cells were mostly of clear cell type. A good rank correlationcoefficient was obtained.
Other cytomorphometric findings concerning keratinizing cells and nuclear area of the cells in each layer had also good correlation with light and electron microscopic findings.View full abstractDownload PDF (14415K) -
Keiko MAJIMA, Mariko KOGANEI, Kumiko NAKAZAWA, Yasue SHITARA, Nobuaki ...1989 Volume 28 Issue 3 Pages 372-377
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDiagnostic utility of cytological examination of sputum in patients with pneumocystic carinii (PC) pneumonia in AIDS was evaluated in comparison with PC pneumonia with hematological disorders. In one case examination was done by bronchoalveolar lavage (BAL), and in 9 by sputum. In BAL case and 7 out of 9 by sputum, cysts of PC were detected. The cysts were clearly demonstrated by Grocott methenamin silver stain. On Papanicolaou stain, they were faintly eosino philic and appeared as ring shape. Other hallmark for PC pneumonia was foamy substance stained by light green, which was consistent with intraalveolar foamy exudate demonstrated in the lung at autopsy. The cysts were in foamy substance in BAL or in cases with abundant foamy material. But in most cases, they were evidenced apart from foamy substance in the background. There was no correlation between the number of cysts demonstrated in sputum and the amount of foamy substance or histiocytes in the background. On the contrary 8 out of 9 PC pneumonia in hematological disorders were diagnosed only by BAL. Thus the sputum cytology is usefull for the detection of PC in AIDS.View full abstractDownload PDF (6991K) -
comparative study of meningiomas, neurilemmomas and astrocytomasShoji KOBAYASHI, Hiroshi MIKI, Masaki OHMORI, Yasunobu FUNAMOTO, Fujio ...1989 Volume 28 Issue 3 Pages 378-383
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSRapid pathological diagnosis during operation is mainly carried out by frozen section technique, but more accurate diagnosis is expected using cytologic studies by squash method. In this report meningiomas and neurilemmomas which have been regarded as not suitable for cytodiagnosis are examined successfully by squash method. In general meningioma cells have abundant cytoplasm and various sized fi laments spreading freely, while neurilemmoma cells have thin cytoplasm and soft slender filament running in the same direction. In cell clusters of meningiomas round nuclei of tumor cells spread at random with some of the nuclei scattered around the cluster, and whorls are sometimes observed in the cluster. In those of neurilemmomas, in contrast, elongated nuclei which are clustered tightly show parallel arrangement, but nuclear palisadings are hardly observed. Both meningiomas and neurilemmomas show prominent layers of tumor cells but not astrocytomas. Squash preparations of astrocytomas show many small blood vessels running among tumor cells.View full abstractDownload PDF (9248K) -
Masahiko FUJII, Kazuo KATO, Yasuyoshi ISHII, Ichiro SAKUMA, Hiroko SAI ...1989 Volume 28 Issue 3 Pages 384-388
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSIn order to elucidate the cytologic characteristics of brain tumors, nuclear features were analyzed in smears taken from 74 human brain tumors. The materials included 8 astrocytomas, 5 anaplastic astrocytomas, 11 glioblastomas, 3 oligodendrogliomas, 3 ependymomas, 5 medulloblastomas, 6 neurilemmomas, 14 meningiomas, 3 germinomas, 9 pituitary adenomas and 7 metastatic adenocarcinomas from the lung. For comparison, 6 specimens of cerebral white matter taken from autopsy cases without neurological diseases were also investigated.
The mean size of the longest axis of nuclei was less than 9 μ in normal astrocytes and gliomas of low grade malignancy. While in malignant tumors such as glioblastoma, germinoma and metastatic adenocarcinoma, many nuclei were more than 10 μ in diameter and irregular in shape.
The nuclear chromatin patterns were finely granular in primary brain tumors and coarsely granular in metastatic adenocarcinomas.
Cells with larger nucleoli than 2 μ in diameter and cells with more nucleoli than 3 were frequently observed in germinomas, metastatic adenocarcinomas and glioblastomas, while these cells were scarcely observed in other histological types.View full abstractDownload PDF (8798K) -
comparative study with a direct smear methodYasuyoshi ISHII, Masahiko FUJII, Tomie WAKABAYASHI, Koji CHYODO, Ichir ...1989 Volume 28 Issue 3 Pages 389-393
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSCells in breast secretion were observed in 11 cases of breast cancer and eight cases of intraductal papilloma obtained by both a cell concentration method and a direct smear method. With the cell concentration method, nipple discharge was collected for sequential three or five days into a glass tube filled with a fixative.
The results were as follows:
1. The cell concentration method yielded a striking increase in the number of cell clusters on smears six times as many as the direct method did.
2. Background of the smears such as red blood cells was more conspicuous in the specimens obtained by the cell concentration method than with the direct method.
3. No significant difference was observed in cellular feature except for nuclear diameter between the two methods.
4. The mean size of the longest axis of nuclei of cells on smears prepared with the cell concentration method was 0.5μ shorter than that prepared with the direct method.
The results show that the cell concentration method is very useful for cytologic diagnosis of nipple discharge, because of obtaining adequate number of cells for detecting atypical cells on smears.View full abstractDownload PDF (7706K) -
Hirofumi KUDOH, Yasuo IITSUKA, Shigemasa KOGA1989 Volume 28 Issue 3 Pages 394-402
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSNuclear DNA of cells imprinted from preoperative biopsy samples, obtained from 125 patients with gastric cancer curative who subsequently underwent operation, was assessed by microspectrophotometry. DNA histogram patterns fell into 4 main categories: diploid type (I), aneuploid type (II), diploid predominant type (III), and aneuploid predominant type (IV): the later two categories being of mosaic type. These four types were further classified into four subtypes, all considered to be polyploid type over 5 c cells, which constituted more than 25% of the total cell count. The relation ship of these 8 types of cells to the progress of cancer, grade of invasion depth, lymphonodal metastasis, histological differentiation grade, and the prognosis of cancer, was analysed. The result of this analysis was that as the stage of gastric cancer progressed, that is, as the grade of invasion depth and lymphonodal metastasis increased, the incidence of poly ploidy cells increased. Cells from those patients with recurrent gastric cancer and/or from patients who died of gastric cancer, often were of aneuploid type. In poorly differentiated cancer, the incidence of aneuploidy or polyploidy increased as the stage of cancer progressed, but the incidence of diploidy on the whole, was high. Well-or moderately-differentiated cancer, early cancer, and cases with stage I cancer, had a high rate of diploid type cells. Howerver this rate was low, when compared with that of poorly differentiated cancer. The incidence of over 5 c cells in each case displayed an upward trend as the stage of gastric cancer progressed. Since it seems apparent that DNA histogram pattens have a relation ship to the stage, histological differentiation grade, and prognosis of gastric can cer, the results of these studies will constitute an index of malignancy grade in gastric cancer.View full abstractDownload PDF (1075K) -
Improvement of the washing containerHiroyuki MIURA, Harubumi KATO, Chimori KONAKA, Norihiko KAWATE, Yoshih ...1989 Volume 28 Issue 3 Pages 403-408
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSRecently the number of central type early lung cancer is increasing. In these cases, it is of importance to obtain specimens by bronchial brushing from small lesions or from where forceps cannot reach. Furthermore differential diagnosis between cancer cells and cis or severe metaplastic cells is important. So the quality of the specimen obtained by bronchial brushing is necessary to help its diagnosis.
We developed a new brush wash kit and investigated its usefulness. Compared with conventional brushing cytology, brushing-washing cytology was found to have the following advantages 1. more number of cells, 2. better cellurarity, 3. no dry specimens, 4. effective screening, 5. possibility of preservation of specimens for other special stains, and the following disadvantages 1. necessity of added process (centrifuge), 2. more expensive.
This method does not prolonged examination time and is harmless and is easy. This method has much diagnostic potential in the diagnosis of lung cancer.View full abstractDownload PDF (7620K) -
Hiromu SHIMIZU, Chikako SHIMIZU, Junko SAITO, Masaki INOUE, Gaiko UEDA ...1989 Volume 28 Issue 3 Pages 409-414
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA rare case of ovarian adenosarcoma was studied with special reference of cytology and histopathology. The tumor developed in the left ovary of a 46-year-old house wife, was first considered to be inoperable. The biopsy specimen revealed an endometriosis like lesion, but with some atypicalities in stromal cells. One year later, the tumor was removed together with the uterus, adnexa, and omentum. Macroscopically, the tumor was polypoid and multiple, weighing 500 g altogether. One cut section, these growths were brownish and mostly solid, but with cystic area in parts. The tumor was consisted histologically of benign-appearing endometriosis glands lying in a cellular and atypical stroma. No tumor cells were found in a scanty ascites, but atypical mesenchymal cells were seen in the imprint smears corresponding to the histological features. Although no mitosis was found, the tumor was diagnosed as adenosarcoma, because the stroma tended to form cellular collars around the glandular structures. The diagnosis was ascertained by examing the recurrent tumor which appeared about three years later. The recurrent tumor was similar macroscopically and microscopically to the original tumor, but with some mitoses (11/10 HPF) in the stromal cells.View full abstractDownload PDF (11725K) -
Yuuko AIZAWA, Yasuko YORINAGA, Yosiko YAMAMOTO1989 Volume 28 Issue 3 Pages 415-419
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSGartner's duct cysts arise from the vestigial remains of the Wolffian canals; the so-called Gartner's ducts. They are generally located on the anterolateral aspect of the vagina. A case of mesonephric adenocarcinoma (clear cell adenocarcinoma) arising from a Gartner's duct cyst was reported. A 46-year-old woman was admitted with complaints of micturition pain and frequent urination, and a cystic tumor located in the anterior wall of the vagina, was detected. Aspiration cytology of fluid from the mass lead to the diagnosis of a class V adenocarcinoma, and cystectomy was subsequently performed. The cystic tumor was located between the urethra and the vagina, and the urethra was surrounded by the tumor circumferentially. The aspiration cytology revealed clear cells, dark cells, and hobnail cells, which are haracteristic features of clear-cell adenocarcinoma. Mirror ball patterns were also identified. The diagnosis based on the histological findings was clear-cell denocarcinoma, and a PAS-alcian blue stain revealed droplets of glycogen in the cytoplasm. The epithelium of the cystic wall consisted of the single-layered or multilayered cuboidal cells of Gartner's duct, and followed the adenocarcinoma continuously. The mucosa of the urethra and the vagina was intact.View full abstractDownload PDF (8536K) -
Kisaku OHNO, Kaiyo TAKUBO1989 Volume 28 Issue 3 Pages 420-424
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA 67-year-old Japanese female with a mucoepidermoid carcinoma of the esophagus is described. Morphological examination was made: the result being that the carcinoma showed a slightly protruded and sclerosing gross appearance. Histologically, the carcinoma contained squamous, adenocarcinomatous and intermediate cells in tumor nests. Imprint cytology demonstrated that many carcinoma cells occurred in sheets and clusters against a necrotic background. They were observed to consist of squamous cells with occasional keratinization, some udenocarcinomatous cells which tested positive for mucin and/or intracellular microcysts, and some intermediate cells having features similar to those of squamous cytoplasm including adenocarcinomatous nuclei. Electron microscopy showed an intracellular microcyst and bundles of tonofilaments in the tumor cell, and these findings suggest that the cell may possess soine characteristics of both squamous and glandular tissues: Immunohistochemical studies resulted in a positive reaction to carcinoembryonic antigen staining, and negative reactions to both secretory component and lactoferrin stainings. The cytological findings mentioned. above suggest the esophageal carcinoma may be a mucoepidermoid carcinoma, even though this is very rare.View full abstractDownload PDF (7143K) -
Kazunori HATA, Kazuo KAWAKAMI, Noboru YAMAMICHI, Masahiro OMORI, Kazut ...1989 Volume 28 Issue 3 Pages 425-430
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe report a rare case of a solid and cystic tumor of the pancreas.
The patient is a 13-year-old girl with a tumor in the head of the pancreas. Based on gross observations and immunocytochemical examinations, the tumor was diagnosed as a solid and cystic tumor. Cytological examinations by the crush method immediately after the operation showed that the tumor cells were columnar with a wide cyanophilic cytoplasmas and biased nuclear. Dysplasia was not observed. The majority had like a columnar shaped with an unclear cell boundary, but a few cells were like an oval or polyhedral with a clear cell boundary. Aggregations of coarse cyanophilic granules were also observed. These granules reacted positively to PAS, α1-antitrypsin, and pancreatic-amylase. Neurospecificenolase reacts showed positive to the cytoplasm of the cells shaped like oval or polyhedral. It seems important to pas special attention to coarse cyanophilic granules stained as well as to the cell shaped like columnar or oval when diagnosing solid and cystic tumor of the pancreas immunocytochemically.View full abstractDownload PDF (13217K) -
Saeko KAMEYA, Aiji NODA, Hitoshi KATO, Shigeki HOTTA, Kazuo HARA, Hiro ...1989 Volume 28 Issue 3 Pages 431-437
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTwo cases of mucin-producing pancreatic tumor were reported and cytological findings were mentioned.
Case 1: A 72-year-old man was admitted because of right hypochondralgia and lumbar pain. Endoscopic examination showed excretion of mucin from a swollen orifice at the minor papilla of Vater. Retrograde pancreatography through this orifice showed diffuse dilation of the main pancreatic duct and filling defect at the head of the pancreas. Brushing cytology of the duct was performed. The cells were seen in large sheets. Most of the cells were round and chromatine was not increased. Overlapping of cells and anisocytosis were slightly observed. Some irregularly arranged sheets were also seen among them. The cytological diagnosis was suspicious of malignancy. Pancreaticoduodenectomy was performed and histopathological findings showed intraductal papillary carcinoma.
Case 2: A 79-year-old man with esophageal carcinoma was found to have a diffuse dilatation of main pancreatic duct on screening examination by abdominal computed tomography. Endoscopic retrograde pancreatography similarly showed a diffuse dilatation of the duct. One year and five months after these examinations, he died of esophageal carcinoma. Autopsy showed a swollen orifice at the papilla of Vater, mucin secretion from the orifice and diffuse dilatation of main pancreatic duct filled with mucin. A few small polypoid lesions were seen in the dilated pancreatic duct of the tail and stamped cytology was done. The cells were round and did not overlap. An anisocytosis was not seen. Cytological diagnosis was benign and histopathological was mucinous cystadenoma.View full abstractDownload PDF (10464K) -
Cytology, ultastructure and immunocytochemistry of five casesNobuzo IWA, Yoshitaka TABARU, Masami IMAKITA, Hatsue UEDA, Chikao YUTA ...1989 Volume 28 Issue 3 Pages 438-443
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThe cytopathologic findings are presented for five cases of adrenal pheochromocytoma. An immunoperoxidase staining technique was also employed in an effort to demonstrate endocrine granule constituents (EGC) in imprint smear as well as tissue sections. The cytology was characterized by tumor cells arranged predominantly in epithelial-like agrregate. The cells had relatively large amounts cytoplasm which was often finely granular. They also revealed multinucleation, cannivalism, vacuolization in many of the tumor cells. In smears as well as tissue section, EGC was immunocytochemically demonstrated intensively on the entire cytoplasm and also in the intranuclear vacuoles. At the ultrastructual levels, the endocrine granules were demonstrated in the cytoplasm of tumor cells with marked electron dense.
The study established the applicability of the immunoperoxidase method for EGC to this endocrine tumors, confirmed the increases accuracy of diagnoses with routine cellular samnles.View full abstractDownload PDF (9664K) -
Cell pathology and cell kineticsToshiaki MORIKI, Machiko HASHIMOTO, Tamotsu TAKAHASHI, Eizi OHHARA, Er ...1989 Volume 28 Issue 3 Pages 444-449
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of malignant mesothelioma of the peritoneum is presented with its cytology, histopathology, immunohistochemistry, electron microscopy and cell kinetics using bromodeoxyuridine (BrdU).
A 50-year-old male was admitted to the Kochi medical school hospital because of ascites, and rt-cervical and ltinguinal tumors were also found. Abdominal CT scanning demonstrated thickened mesenterium and retroperitoneum. Cytology of peritoneal effusions and aspirates from these tumors revealed that numerous mesothelial cells with large papillary clusters contained multinucleated giant cells. A small numbers of intranuclear inclusions were also found. Histologically, biopsy specimens of these tumors were metastatic epithelial type of mesothelioma. Immunohistochemical staining with ABC method showed that the tumor cells were reacting positively for EMA, Vimentin and Keratin, however, negatively for CEA. Ultrastructural studies had demonstrated that the tumor cells had epithelial characteristics with numerous complex and elongated microvilli (MLDR=17-19). The percentage of BrdU positive mesothelioma cells was about 7% and it was higher than that of reactive mesothelial cells and macrophages obtained from other cases.
It is sometimes difficult for identified the malignant epithelial mesothelioma cells in effusion cytology. However, it is important to find the nature of mesothelioma cells with special stains for hyaluronic acid, immunohistochemistry and electron microscopy. The findings of intranuclear inclusions and detection of cell kinetics using BrdU method may be useful for distinction between reactive mesothelial cells and malignant epithelial mesothelioma cells.View full abstractDownload PDF (10070K) -
Tetsuro NAGASAKA, Nobuo NAKASHIMA, Toshiaki FUKATSU, Shinji FUKATA, No ...1989 Volume 28 Issue 3 Pages 450-454
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of malignant histiocytosis is reported. A 6-year-old boy was admitted to our hospital, complaining of bilateral cervical lymphnode swelling accompanied by fever. A lymphnode was biopsied, and immediately an imprint preparation and a frozen tissue section were made. Cytologically, scattered atypical cells, with basophilic cytoplasm an increased amount of chromatin, and slightly indented nuclei with prominent nucleoli, were found to be intermingled with small lymphocytes. Erythrophagocytosis of atypical cells was observed frequently. We concluded that these atypical cells were histiocytic tumor cells. In the frozen tissue section, vigorous proliferation of the atypical cells formerly seen in the imprint preparation, was observed in the parafollicular area and the medullary sinus of the lymph node. The diagnosis of malignant histiocytosis was made based on the combination of cytological and histological findings. In this case, cytological findings from the imprint preparation played a very important role in the rapid diagnosis of the disease.View full abstractDownload PDF (6398K) -
Masatomo KIMURA, Shunji MAEKURA, Eizi KADOTA, Yasuro OBANA, Kuniyasu S ...1989 Volume 28 Issue 3 Pages 455-458
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSCandida, one of yeast-like fungi, is a saprophyte of the oral cavity and the vagina, and acting opportunistically, it often produces abscesses in the internal organs. In this report of two cases of disseminated internal candidiasis, a smear was found to be more useful for the detection of the Candida itself, than was a histology preparation. Case 1 was a recurrent case of acute myeloid leukemia. Candida abscesses were found in the lungs, liver, kidneys, and spleen, and a Candida verruca was found in the left ventricle on autopsy. Case 2 was observed at the autopsy of a patient after acute myocardial infarction. Candida abscesses were found in the kidneys and in the anterior wall of the left ventricle. In both of these cases, Candida could not be clearly observed in histology preparations, because of the dense proliferation of the Candda itself and/or infiltration by inflammatory cells. However, in the smear, the characteristic pseudohyphae and blastoconidia of Candida were easily observed. Each smear, made from fresh and formalin-fixed abscess material, demonstrates the same fungal view ; a smear should be made even after formalin-fixation of the abscess.View full abstractDownload PDF (6749K) -
Hideo TESHIMA, Tsugukatsu NASHIRO, Hiroyuki FURUTA, Morio HIRATA, Sach ...1989 Volume 28 Issue 3 Pages 459-461
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (3504K) -
Motoko IKENAGA, Atsuhiko SAKAMOTO, Masafumi TSUZUKU, Tomoyoshi KAWAGUC ...1989 Volume 28 Issue 3 Pages 462-463
Published: 1989
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (3132K)
- |<
- <
- 1
- >
- >|