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Volume 29 (1990)
- Issue 6 Pages 793-
- Issue 5 Pages 635-
- Issue 4 Pages 487-
- Issue 3 Pages 363-
- Issue 2 Pages 143-
- Issue 1 Pages 1-
Volume 29, Issue 4
Displaying 1-16 of 16 articles from this issue
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Yasuyoshi ISHII, Masahiko FUJII, Risa ITOKAZU, Koji CHYODO, Ichiro SAK ...1990 Volume 29 Issue 4 Pages 487-492
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSIn order to investigate the architectural abnormalities of well differentiated endometrial adenocarcinoma, glandular tubules and papillary projections were examined by threedimensional reconstruction based on 100 serial sections owing to 5μm thickness. Glandular tubules of adenomatous hyperplasia and normal endometrium were also examined by 3-D reconstruction to compare them from cancer.
Normal endometrium in 3-D reconstruction has a simple tubular architecture with few branches, while, adenomatous hyperplasia has a multiply protrusive 3-D glandular pattern. On the other hand, well differentiated adenocarcinoma has an extremely complex glandular and papillary pattern with irregularly ramificating branches showing a tree-like appearance.
These observations are consistent with the cytologic findings that marked ramificating protrusions from cell cluster are not infrequently present in case of well differentiated endometrial adenocarcinoma.View full abstractDownload PDF (9674K) -
Noriyuki FURUTA, Hideo TESHIMA, Morio HIRATA, Kazuhiro YAMAUCHI, Katsu ...1990 Volume 29 Issue 4 Pages 493-499
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe have studied to diagnose the HPV infection of the uterine cervix by cytology.
1) We used the Dot blot Hybridization method to detect the HPV DNA genome.
2) We have studied cytological specimen, which had proved HPV genomes positive.
3) Results: The incidence of cytological findings of HPV infected cells were; parakeratosis in 100%(72/72), koilocytosis in 60%(43/72), smudged nucleus in 60%(43/72), giant cell in 21%(15/72), multinucleation in 63%(45/72), condylomatous parabasal cell in 28%(20/72) and amphophilia in 55%(40/72). In conclusion, to diagnose the HPV infection by cytology it seems very important to find out such findings as parakeratosis, koilocytosis, smudged nucleus and giant cell.View full abstractDownload PDF (6303K) -
with reference to their originMinoru UEKI, Akio KUROKAWA, Masao MORIKAWA, Tadashi KUSAKABE, Kousuke ...1990 Volume 29 Issue 4 Pages 500-508
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTo characterize tissue repair cells (TR) of the uterine cervix and clarify their origin, cells exfoliated following laser conization for CIN were examined. One specimen plate was first examined after Papanicolaou (Pap) staining and then examined after immuno-histochemical staining using the EMA and vimentin restaining method. An other specimen plate was observed after PTHA staining. The findings thus obtained were investigated together with the histologic findings of the wound healing process reported previously.
After Pap staining TR were classified morphologically into three groups: stromal TR (STR), epithelial TR (ETR) and TR of unknown origin (UTR), respectively. The validity of this classification was suggested by the results of immuno-histochemical staining with EMA and Vimentin. These cells appeared in one to eight weeks. TR with very large nucleus, referred to as ATR appeared when all types of TR were most plentiful at two to five weeks. Regarding the origin of each type of TR, cytological and histological findings suggest that ETR are immature squamous epithelial cells and reserve cells under columnar epithelia. On the other hand, the presence of myofibrils in the cytoplasm was demonstrated by PTAH staining for STR and some UTR strongly suggesting the possibility that these cells are myofibroblasts in granulation tissue. This finding corroborates the existence of STR in addition to ETR as inferred by Bibbo.View full abstractDownload PDF (8904K) -
Kazuo Kato, Naoki Yoshimi, Tokuro Shinoda, Hitoshi Iwata, Akira Hara, ...1990 Volume 29 Issue 4 Pages 509-515
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe have established a new cell line (C-1509) producing a glycoprotein, CA 54/61 derived from a large cell undifferentiated carcinoma of the lung (LCUCL). A long-term and stable proliferation has been maintained for more than 150 successful passages over a 5-year period. The C-1509 cell population doubling time (DT) was 25-38 h, saturation density was 1.6×105 cells/cm2. The microscopic features of the tumors which were formed in athymic mice by subcutaneous injection of C-1509 cells were histologically similar to the original large cell carcinoma.
In culture, the production of the glycoprotein, CA 54/61, increases with cell proliferation and reaches a peak level just before the plateau phase proliferation.View full abstractDownload PDF (9676K) -
Yoshimi MIZUNO, Hiroshi SUGIURA, Kazuo HARA1990 Volume 29 Issue 4 Pages 516-521
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of Merkel cell carcinoma was studied cytopathologically, immuno-histochemically and ultrastructurally.
66-years-old woman noticed a small nodule on her right cheek, which was biopsied and referred to Aichi Medical University under the diagnosis of malignant lymphoma. Three months later she felt a mass in the right neck region. Lymph node biopsy revealed metastatic Merkel cell carcinoma of the lymph node.
Cytologically, tumor cells were 1.5 to 2 times as large as small lymphocyte. Nuclei were round with several small nucleoli. The chromatin was fine and granular. Cytoplasm was scanty. N/C ratios were large. Most tumor cells were singly isolated, but small clusters, suggestive of epithelial character were also found.
Ultrastructurally there were dense-core granules, 100nm in diameter in the cytoplasm. Immuno-histochemically tumor cells were positive for keratin and NSE, and negative for vimentin, LCA, S-100 protein and EMA.View full abstractDownload PDF (10604K) -
its cytologic featuresShigeo NAKAMURA, Sadayuki KABA, Kuniyoshi KITO, Taizan SUCHI, Soji KUR ...1990 Volume 29 Issue 4 Pages 522-525
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThe imprinted cytologic findings of an interdigitating cell sarcoma, a very rare malignancy, in a 58-year-old man are presented. A series of investigation described previously allowed determination of an interdigitating cell origin to be made. Cytologically, atypical cells, often with cannibalism, were found to be intermingled with neutrophiles and small lymphocytes. The specimen showed a cytomorphologic spectrum that varied from epithelioid-like cells to multinucleated and/or multilobulated large tumor cells. The tumor cells had the characteristic pleomorphic nuclei with prominent indentation. Furthermore, May-Giemsa stain revealed a number of azurophilic granules in the cytoplasm of a part of the neoplastic cells. These findings suggested that the imprint preparation played an important role in the diagnosis of the disease.View full abstractDownload PDF (4163K) -
Hironobu ADACHI, Haruhiko YOSHIDA, Takeshi MINAMIZAKI, Shoji YASHIMA, ...1990 Volume 29 Issue 4 Pages 526-529
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSEpithelioid osteosarcoma of the right femur in a 17-yearold Japanese male was reported.
Cytological examinations with imprinted specimens from the intra-and extra-osseous tumors revealed clusters of polyhedral cells with round nuclei and pleomorphic cells with round to fusiform nuclei. Multinucleated bizarre giant cells were also scattered. Neither osteoid nor allied substances were observed.
The clustered epithelioid cells with round nuclei were not initially interpreted as osteosarcoma cells but as epithelial tumor cells. Histochemically, both types of tumor cells showed strong positivity for alkaline phosphatase. Histologically, the tumor mass was composed of a nest of polyhedral tumor cells with fine fibrovascular stroma without osteoid structures. However, because the lacelike osteoids closely associating with round and fusiform tumor cells were observed, a final diagnosis of osteosarcoma was given.
This case suggests that cytological diagnosis of osteosarcoma should be based upon a combination of complete clinical data and enzyme histochemical examinations.View full abstractDownload PDF (7451K) -
Taku KATOH, Hisao TAKAHASHI, Yukitoshi SATOH, Bin TAKEDA, Hitoshi KUBO ...1990 Volume 29 Issue 4 Pages 530-534
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of malignant leiomyoblastoma of the stomach in a 85-years-old female is presented. The tumor was located in the submucosal layer on greater curvature, and 6×5×4cm in size with ulceration. Tumor cells were small, round or oval, cyanophilic or naked, isolated and scattered.
Histology of the tumor showed leiomyoblastoma with frequent mitotic frigures, arising from the muscular layer, partly invading blood vessels and destroying lamina muscularis mucosae. Tumor cells were positive for Vimentin, negative for Grimelius, PAS staining, EMA, Keratin, S-100, Desmin by immunohistochemical study.
Electron microscopic finding indicated that the tumor originated in the smooth muscle with pinocytotic vesicles, basal lamina, hemidesmosome in dark cell.View full abstractDownload PDF (10846K) -
Moriyasu OIKAWA, Shunichi SASOU, Tamotsu SUGAI, Junko SASAKI, Kazuo TA ...1990 Volume 29 Issue 4 Pages 535-538
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe report a case of adrenocortical carcinoma and discuss various criteria for diagnosis of adrenocortical carcinoma.
A 40-year-old woman was found to have an enlarged liver. CT scan and angiological examination suggested that the tumor was a malignant adrenal tumor. The tumor weighed 560 g and was encapsulated. The cut-surface was yellowish in color with necrotic lesions. Tumor cells were found in clusters and as scattered isolated cells in an imprint smear. The tumor cells were of medium size, had concentric and/or eccentric round nuclei with hyperchromatism and foamy or vacuolated cytoplasm. The nuclei were round, but frequently showed slight variation in shape and had irregular borders. Mitotic figures were occasionally found. Immunohistochemically, the tumor cells were vimentin and cytokeratin positive. Histologically, the tumor was composed of varying proportions of eosinophilic compact cells and clear cells with foamy cytoplasm. The nuclei were often irregular in shape. Mitotic figures were rarely present. Necrotic lesions and degenerative changes were found in the tumor cells. Capsular and vascular invasion of the tumor was demonstrated.View full abstractDownload PDF (7547K) -
Gita Sayami, Yoshiro Ebihara, Hiromi Serizawa, Harubumi Kato, Yoshihir ...1990 Volume 29 Issue 4 Pages 539-543
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA 17-year-old male with effusion in the pleural cavity and a tumor on the left 7th rib was diagnosed by exfoliative cytological and histological examination of pleural fluid and biopsy specimen from the chest wall tumor.
In the pleural effusion, small round cells with nuclear atypia were demonstrated as a mixture of monolayered clusters and single cells, with scanty and indistinct cytoplasm. The nucleus was round, supported by thin and smooth nuclear membrane, and hyperchromatic, showing finely granular chromatin pattern with one or two small but distinct nucleoli. PAS stain demonstrated intracellular glycogen. Histologically, biopsy specimen from the chest wall tumor yielded a diagnosis of round cell tumor, compatible with Ewing's sarcoma.
Differential diagnosis of small round cell tumors was discussed from a morphological point of view.View full abstractDownload PDF (6345K) -
Hirofumi SAKURAI, Osamu YAMAGAMI, Jun NAKAYAMA, Tsutomu KATSUYAMA, Mas ...1990 Volume 29 Issue 4 Pages 544-547
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA rare case of squamous cell carcinoma arising in a median cervical cyst was reported. A 57-year-old male with a neck tumor had been diagnosed clinically as having a median cervical cyst or ectopic thyroid on the basis of ultrasound examinations. Aspirates of the tumor, however, showed a great number of atypical clusters with marked keratinization. After radiation therapy, the tumor was totally resected.
Macroscopically, the tumor was cystic and communicated with the thyroglossal duct. Histological examination revealed moderately differentiated squamous cell carcinoma with marked invasion. The simultaneous presence of intraepithelial extention of the carcinoma cells and normal ciliated epithelia in the cyst wall suggested that the carcinoma cells were primarily derived from the median cervical cyst.
In this case, aspiration cytology contributed to adequate therapy. Although squamous cell carcinoma of a median cervical cyst is uncommon, neoplastic change may be diagnosed by routine cytological examinations.View full abstractDownload PDF (6577K) -
scanning and transmission electronmicroscopic findingKenichiro SATO, Ryuichi KUDO, Hidemitsu MIZUUCHI, Takaya OKAZAKI, Tomo ...1990 Volume 29 Issue 4 Pages 548-554
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of clear cell carcinoma of the uterine endometrium is presented with its cytology, histopathology, transmission electronmicroscopy (TEM) and scanning electronmicroscopy (SEM). As to its cytology:(1) the cytoplasm was rich, cyanophilic and clear;(2) all nuclei were relatively the same size with a fine granular patterned chromatin and tended to stand to one side of the cytoplasm;(3) the one or two nucleoli were eosinophilic;(4) overlapping was slight with a rosette-like or pavement arrangement and;(5) several hobnail type cells were observed.SEM showed that the hobnail type cells had a few short, well spaced microvilli, which stood straight. The surface of the round carcinoma cells had many relatively long microvilli. TEM showed that the hobnail type cells had rough endoplasmic reticulum around the mitochondria, fat drops, lysosomes, a narrow glycogen's field.View full abstractDownload PDF (15629K) -
Kiyoko ICHIDA, Toshiyuki KUBO, Ichiro NAKAMOTO, Kazuaki INOUE, Kenji S ...1990 Volume 29 Issue 4 Pages 555-561
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSPrimary carcinoma of the Fallopian tube is the least frequent of all malignant tumors of the female genital tract. This report deals with a case of poorly differentiated adenocarcinoma of the Fallopian tube. In the preoperative diagnosis, cervico-vaginal cytology played a significant role.
A 46-year-old patient visited the gynecological department of Biken Hospital with the complaints of waterery yellowish pink discharge and intermittent lower abdominal pain. On the basis of the results of cervico-vaginal cytology strongly suggesting malignancy and the findings of ultrasonic and CT examination of the pelvis, a malignant Fallopian tube tumor was highly suspected and operation was carried out.
Cytological evaluation of the peritoneal effusion revealed that the cells were of adenocarcinoma origin, and the histological diagnosis of the resected specimen was poorly differentiated adenocarcinoma originating in the Fallopian tube.
Imprinting cytology demonstrated a microgland-like structure filled with PAS-and alcian blue-positive material. Electronmicroscopic observation was also carried out. The cancer cells formed small glands with short microvilli projecting into the glandular lumen. These structures seemed to correspond to the microgland pattern seen in light microscopic observation. The cytoplasm often contained membrane-bound granules suggesting secretory substances.View full abstractDownload PDF (7576K) -
immunohistochemical and ultrastructural studiesTomoyuki MIYAKAWA, Tsutomu KOIZUMI, Yoshimi INANIWA, Noriko KINUKAWA, ...1990 Volume 29 Issue 4 Pages 562-567
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA rare case of endodermal sinus tumor of the vagina is reported. The patient, a 9-month-old female, was admitted to the hospital with a chief complaint of vaginal bleeding. A vaginal tumor was found and was discharged spontaneously.
Imprint smears of this tumor revealed pleomorphic malignant cells occurring in irregular or sheet-like clusters with ill-defined cytoplasmic borders. PAS-positive hyaline globules were seen in and around the tumor cells. These findings suggested a diagnosis of endodermal sinus tumor. Histologic examination revealed the typical reticular or microcystic pattern of endodermal sinus tumor with Schiller-Duval bodies and hyaline globules. Immunohistochemically, there was a positive reaction for AFP in the cytoplasm of the tumor cells. Positively stained globules were also identified. Ultrastructurally, small acinar lumina surrounded by tumor cells with microvilli, desmosome junctions and electron-dense basement membrane material were noted.
Most malignant tumors of the vagina in infants and children are rhabdomyosarcomas, but endodermal sinus tumor and clear cell carcinoma are seen rarely. Imprint smears appear to be useful in the differential diagnosis of these tumors.View full abstractDownload PDF (11117K) -
Yoshimune HORIBE, Masao KASAHARA, Hiroshi HIRASAWA, Masanori FUNAHASHI ...1990 Volume 29 Issue 4 Pages 568-569
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (3818K) -
Evaluation of the atypical cells originated from the benign folliclesHitoshi NAKAGOMEI, Makoto KAWAMURA, Hiromitsu MATSUSHIMA, Yoshitaka KA ...1990 Volume 29 Issue 4 Pages 570-571
Published: 1990
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (2748K)
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