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Volume 32 (1993)
- Issue 6 Pages 833-
- Issue 5 Pages 651-
- Issue 4 Pages 481-
- Issue 3 Pages 365-
- Issue 2 Pages 157-
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Volume 32, Issue 4
Displaying 1-25 of 25 articles from this issue
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Mika SHIRAHAMA, Tooru WATANABE, Iwao EMURA, Makoto NAITO1993 Volume 32 Issue 4 Pages 481-488
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSIt was found that almost all keratotic and parakeratotic cells showed birefringence under polarized light. Other types of cells, such as endometrial stromal cells, glandular epithelial cells, adenocarcinoma cells, and non-keratinizing squamous cell carcinoma (SCC) cells, did not show birefringence under polarized light, and were therefore easily differentiated from most keratotic and parakeratotic cells. It was therefore concluded that polarizing microscopy is quite useful in identifying keratotic and parakeratotic cells. Parakeratotic cells exhibiting birefringence were classified into two subgroups, cells having a pyknotic nucleus (K, P-CELLs) and cells having a non-pyknotic nucleus (K, NP-CELLs). The nuclei of K, P-CELLs and K, NP-CELLs were smaller than those of non-keratotic cells. We consider the following three criteria to be important in the cytological diagnosis of keratinizing SCC.
1) Presence of both K, P-CELLs and K, NP-CELLs.
2) K, P-CELLs with nuclei having an area larger than 18.4, μ2.
3) K, NP-CELLs with degenerated nuclei (vacuolated aggregated chromatin pattern) having an area larger than 31.1, μ2.
In some cases, it was difficult to distinguish keratotic SCC in situ from invasive keratotic SCC or keratotic severe dysplasia from keratotic SCC in situ on the basis K, P-CELLs and K, NP-CELLs.View full abstractDownload PDF (9505K) -
Yusuke MATSUURA, Toshinori KAWAGOE, Michioki SHINOHARA, Masamichi KASH ...1993 Volume 32 Issue 4 Pages 489-494
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDuring the eight years from 1984 to 1991, 1717 pregnant women were examined using the routine Papanicolaou test. Twenty-six (1.5%) showed abnormal cytologic results. Colposcopically-directed punch biopsies revealed cervical dysplasia and CIS in 14 patients (0.82%). Severe dysplasia and CIS were seen in 8 patients, including an additional 4 referred patients. Different interpretations of cytology and punch biopsy were made in 7 of the 8 cases. Severe glandular involvement of atypical epithelia was observed in 5 cases.
In 6 of the 8 cases, the lesion disappeared after delivery. Overevaluation of the punch biopsy specimens during pregnancy may be the main reason for this change.
The incidence of dysplasia and CIS is the same in pregnant and nonpregnant women, so the routine Papanicolaou test must be performed during pregnancy.View full abstractDownload PDF (4546K) -
Yasuhisa IWAOKI, Tuyuko YAMAMOTO, Jun AOKI, Yasuhiro KATSUBE, Koji NAN ...1993 Volume 32 Issue 4 Pages 495-501
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSAmong the 2346 endometrial cytodiagnostic studies performed at Kure Kyosai Hospital from 1987 to 1990, there were 7 false positives (0.3%) and 5 false negatives (0.2%). These misdiagnosed specimens were rescreened by 16 certified cytotechnologists at other hospitals and reexamined.
Of the 7 false-positive cases among the 44 positive cases, 4 were ultimately diagnosed as atrophic endometrium, 1 as IUD endometrium, 1 as syncytial endometritis, and 1 as normal endometrium.
There were 19 cases of endometrial carcinoma, 5 of which were judged negative, 3 suspicious, 9 positive, and 2 inadequate. Among the 5 false-negative cases, 4 were welldifferentiated endometrial adenocarcinoma and 1 was adenoacanthoma.
As a result of rescreening, the cases of atrophic endometrium were judged positive by 21% of the cytotechnologists, the IUD case as positive by 13%, the syncytial endometritis as positive by 7%, the degenerated normal endometrium as positive by 50%, the well-differentiated adenocarcinoma as negative by 44%, and the adenoacanthoma as negative by 81%. The length of time the cytotechnologists had been certified had no effect on their decisions except in the case of atrophic endometrium.
Reexamination of cytology and histology revealed that the false diagnosis was caused by atypical cells resembling regenerative epithelium in the cases of atrophic endometrium and syncytial endometritis, by poor cytological criteria and no information in the IUD case, by the presence of degenerative cells in the case of normal endometrium and by a sampling error in the case of adenoacanthoma. Diagnosis was difficult in some cases of atrophic endometrium and welldifferentiated adenocarcinoma.View full abstractDownload PDF (7232K) -
Youko SASAKI, Shouichi HOSOYA, Shinji TANIO, Shu NAKAMOTO1993 Volume 32 Issue 4 Pages 502-506
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe analyzed cytodiagnoses based on 125 samples of PTCD (percutaneous transhepatic cholangiography and drainage) fluid from 89 patients at Tottori Prefectural Central Hospital during the five years from 1986 to 1991. Diagnoses were comfirmed by histological examination and/or by the patients' course.
1. The results of PTCD-cytodiagnosis were excellent. The validity rate was 74.4% as a whole and rose to 85.9% when cases in which there was failure to obtain evaluable cells were excluded.
2. The important findings in PTCD-cytodiagnosis were loose cellular connections, variable internuclear distance, thickening of the nuclear rim, necrotic background and an increase in nuclear chromatin. When average nuclear size, the most objective finding in cytology, exceeded a diameter of 9μm the probability of malignancy was extremely high.
3. Twenty-four false-negative tests were demonstrated, 4 cases of under evaluation of the cellular picture and 20 cases of unsuccessful collection of evaluable cells.
4. There were 8 false-positive tests. In spite of the review, 4 yielded cytologically malignant findings.
5. In 40 tests (32%) there was failure to obtain proper cells. It is important to improve the method of collecting PTCD fluid, which would rise the accuracy of PTCDcytodiagnosis.View full abstractDownload PDF (2944K) -
A feature of aspiration cytologyMitsuyoshi HIROKAWA, Hiroe NISHIMURA, Isao IREI, Yoshiki MIKAMI, Yasum ...1993 Volume 32 Issue 4 Pages 507-510
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe examined aspiration cytology specimens from 16 cases of thyroid papillary carcinoma associated with ossification. The true aspiration cytology positive rate (47%) was low. Cases with a large degree of ossification tended to yield less aspiration material. Even when aspiration for a hard mass in the thyroid is negative, we like to think we still have to consider the possibility of malignancy. There was no bony tissue in 19 aspirated materials, but in 3 cases there was adipose tissue composed of fat cells of a variety of cell size and foamy cytoplasm. The presence of adipose tissue may be indicative of aspiration from a papillary carcinoma with ossification.View full abstractDownload PDF (5090K) -
Yasunobu FUNAMOTO, Shoji KOBAYASHI, Akira SATOH, Fujio KISHIDA, Kohji ...1993 Volume 32 Issue 4 Pages 511-520
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSImprint cytology of Hodgkin's disease was assessed in 20 cases, including 5 of lymphocyte predominance, 7 of mixed cellularity and 8 of the nodular scleosis type of disease, all of which were confirmed by histological examination. Acid phosphatase (ACP), alpha-naphthyl acetate esterase (alpha-NAE) and immunocytochemical staining was performed in some of the cases in addition to Papanicolaou and May-Giemsa stains in all of them. Cytologically, the common characteristics of Hodgkin cells (H cells) and Reed-Sternberg cells (RS cells) for all subtypes of Hodgkin's disease were flexible-appearing and faintly-stained nuclei with evenly and deeply stained nucleoli, But variations in the size and shape of the cells and some degree of pleomorphism in nuclei and nucleoli were observed. The results of ACP and alpha-NAE staiing demonstrated that H cells and RS cells exhibit various findings in the cytoplasm from faintly but diffusely positive and strongly positive staining in the Golgi area to completely negative staining. Immunocytochemically, H cells and RS cells were positive for L-26 in 4 out of 15 cases, for Leu M 1 in 4 out of 16, and for Ki-1 in 8 out of 14. It is interesting that some histiocyte-like or reticulum cell-like non-H and non-RS cells were positive for Ki-1 and Leu M 1. In the background, T cells were predominant in almost all of the cases, except for lymphocyte predominance, in which B cells were predominant. We conclude that imprint cytology will be very useful for the identification of H cells and RS cells and for the diagnosis of Hodgkin's disease.View full abstractDownload PDF (16462K) -
Using prostatic and osseous biopsy materialsRitsuko IMAI, Kouji SATO, Tetsuo MURAMATSU, Masanori YAMAMOTO, Toshiak ...1993 Volume 32 Issue 4 Pages 521-525
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSIt is generally difficult to make stamp smears and quick tissue preparations from osseous or very small biopsy specimens. In this paper the authors report a new method of making cytologic preparations quickly from these tissues.
The material was three osseous biopsies (metastatic lesions from small cell carcinoma and adenocarcinoma of the lung, and from renal cell carcinoma, respectively) and 17 prostate biopsies (three adenocarcinomas and 14 non-cancerous lesions).
Each biopsy specimen was put into a centrifuge tube containing saline supplemented with five or six drops of 22% albumin solution and shaken lightly to float the cells out of the tissue. The tissues were then removed and processed by routine procedures for making histological preparations.
Cytologic preparations were made by centrifuging saline at 1500 rpm for 5 minutes or using autosmears and stained by the quick Papanicoloau staining method.
Six cases of cancer were all diagnosed correctly by the cytological method. A sufficient number of cancer cells were observed in the smears. Cells and nuclear shrinking or nuclear isolation were observed, but the degree was not severe. Accordingly, this method is thought to be very useful in the rapid diagnosis of biopsy material taken from the organs above.View full abstractDownload PDF (7173K) -
A case reportKazuchiyo Iemuran, Kazuo Ohmi, Takahiko Sonoda, Ryuichiro Tsunematsu, ...1993 Volume 32 Issue 4 Pages 526-530
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of small cell carcinoma of the uterine cervix, in which peritoneal washing cytology (PWC) was useful for evaluating the extent of cancer spread, is reported. Preoperative smear cytology of the cervix showed that the cancer cells were immunoreactive with anti-chromogranin A antibody and NCC-Lu 243, which is a monoclonal antibody directed against cluster 1 small cell lung cancer antigen. The clinical stage of the disease was Ib, and the patient underwent radical hysterectomy. Although no ascitic fluid was present and peritoneal dissemination was not evident macroscopically, intraoperative PWC demonstrated numerous small cell carcinoma cells. The pelvic lymph nodes did not appear to be swollen, but pathological examination of a surgically resected specimen revealed cancer metastasis. This case suggests that PWC would be very useful for accurate evaluation of the microscopic extent of cancer spread at the time of surgery for small cell carcinoma of the uterine cervix.View full abstractDownload PDF (7128K) -
Michioki SHINOHARA, Yuusuke MATUURA, Masamichi KASHIMURA1993 Volume 32 Issue 4 Pages 531-535
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSSclerosing stromal tumor (SST) is an uncommon benign ovarian neoplasm principally occurring in young women. Although 91 cases of SST have been reported in the literature, the cytologic findings have not been described. A cases of SST in a 13-year-old Japanese girl is presented in this paper, and this is the youngest patient and the largest tumor. This neoplasm did not display hormonal activity and exhibited typical microscopic findings. The cytologic findings of SST are reported for the first time. Isolated, small cells without cytoplasm, having round or spindle-shaped nuclei with a thin nuclear membrane, finely granular chromatin, and single or double small nucleoli were found in the imprint cytology sample. Although SST can be pathologically differentiated from theca cell tumor and fibroma, prospective cytologic diagnosis of SST is considered difficult. The clinical features of 62 cases of SST are also described in detail based on a review of the literature.View full abstractDownload PDF (6532K) -
Rinya SATOH, Toshiko JOBO, Yoshiki KIDA, Shinpei TUNODA, Takao SHIMODA ...1993 Volume 32 Issue 4 Pages 536-542
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of serous surface papillary carcinoma (SSPC) with multiple primary malignancies (initially uterine cervical carcinoma 16 years ago and secondly breast cancer 5 years ago) is reported. The patient was a 83-year-old woman who complained of significant abdominal distension due to abundant ascites. Cisplatin-based chemotherapy was tried but had no effect. In cytological specimens of the ascitic fluid, the tumor cells were observed forming papillary clusters with prominent stratification, and obvious anisonucleosis and pleomorphism were present. There was a fine chromatin pattern, and 1 or 2 small nucleoli were seen. The cytoplasm was scant and stained light green, while some tumor cells had small vesicles without any PAS-positive granules. At autopsy, gross peritoneal seeding was seen, and bulky lesions involved the omentum, however both ovaries were of normal size and shape. The tumor histology closely resembled that of serous papillary carcinoma of the ovary, and psammoma bodies were present. The tumor was widely disseminated throughout the abdominal cavity, but neoplastic involvement of the ovaries was confined to the surface.
Electron microscopic examination demonstrated rare tonofilaments.View full abstractDownload PDF (8667K) -
Miki KUSHIMA, Hiroyoshi OKI, Norio KAWASE, Masafumi TAKIMOTO, Akira SH ...1993 Volume 32 Issue 4 Pages 543-546
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTwo cases of secretory carcinoma of the breast, in a 55-and 53-year-old woman, are reported. Aspiration biopsy cytology revealed some characteristic features. Many malignant cells were scattered in Papanicolaou smears from case 1, with mild atypia. A few clusters of more atypical cells were observed in smears from case 2. So-called “mucous globular str uctures (MGS) ”, “clusters like bunches of grapes” and “punched out lesions” were observed in the smears from both cases.
Histologically, a typical pattern of secretory caricnoma was seen in the histologic mastectomy specimens from both cases and partial atypical features of a signet-ring cell carcinoma-like pattern were found in case 2.
In cytologic diagnosis, it is important to find “MGS” and “bunches of grapes” in as piration smears of secretory carcinoma of the breast.View full abstractDownload PDF (3411K) -
Setsuo SUGISHIMA, Toshiro YOKOYAMA, Tomoko YOSHIDA, Hiromi TAKAGI, Tos ...1993 Volume 32 Issue 4 Pages 547-551
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe report a 65-year-old female with basal cell epithelioma (basal cell carcinoma) of the breast who was suspected of having ductal carcinoma as a result of fine-needle aspiration cytology.
The patient was admitted to our hospital because of a right breast tumor. The tumor measured 1.5 cm in diameter, was located in region C and showed no obvious changes in size for ten years.
The fine-needle aspiration smear showed several large and small clusters of tumor cells against a hemorrhagic background. The tumor cells were in tight clusters with nuclear overlapping and hyperchromasia. We suspected ductal carcinoma on the basis of preoperative cytology, however, the histological diagnosis was basal cell epithelioma.
The characteristic finding of basal cell epithelioma cytology, palisading arrangement, was observed at the periphery of the clusters of tumor cells. The nuclei were small and round with no anisokaryosis.View full abstractDownload PDF (7691K) -
Nobuyasu NISHISAKA, Masaomi SASAKI, Kenichi WAKASA1993 Volume 32 Issue 4 Pages 552-556
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe report a case of malignant lymphoma of the prostate and the root of penis with appearance of tumor cells in the urine. A 81-year-old man complained of pain on initiating micturition and a mass in the root of penis. Since urinary cytology revealed malignant lymphoma cells, thorough examination was performed, resulting in the diagnosis of malignant lymphoma of the prostate. The prostate biopsy specimen revealed infiltration by atypical lymphocytes consisting of centrocytes with cleaved cells. Immunohistochemically, the tumor cells were positive for UCHL-1 but negative for L 26, indicating malignant lymphoma of T-cell origin. The patient was treated with three couses of CHOP chemother apy, but died 18 months ater of brain metastasis. Malignant lymphoma of the prostate, whether primary or secondary, is uncommon and is rarely detected on urinary cytology. However, urinary cytology appeared to be useful as a screening test for malignant lymphoma of the prostate.View full abstractDownload PDF (6911K) -
Takao SATOU, Motohiro IMANO, Akio KUBOTA, Harumasa OHYANAGI, Tadao UES ...1993 Volume 32 Issue 4 Pages 557-561
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSHistological examination is important for the differential diagnosis of mesenteric cysts. Aspiration cytology is considered only a method of morphological observation for preoperative diagnosis. We report a case histopathologically diagnosed from surgical specimens as cystic lymphangioma of a mesenteric cyst. The patient was a one-year-old girl who presented with abdominal distention and fever. A diagnosis of intra-abdominal cyst was made, and she was treated surgically. The cyst occupied almost the entire intraabdominal space. The cyst contents were bloody, and the inner surface of the cyst had a trabecular appearance with a septum. The histopathological diagnosis was cystic lymphangioma based on the presence of the endothelium on the inner surface of the cyst. Abundant lymphocytes and lymphoid follicles were revealed in the cyst wall. Aspiration cytology was performed three times preoperatively. Many small lymphocytes and a few scattered large lymphocytes were revealed in the cytological specimens. The large lymphocytes had atypical irregular nuclear shapes and chromatin patterns. No columnar epithelium, cuboidal cells or endothelium were detected. The large lymphocytes were considered to originate from the germinal center of the lymphoid follicles. The present case demonstrates that aspiration cytology is useful in the preoperative diagnosis of mesenteric cyst, because lymphangioma can be assumed if an intra-abdominal cyst is revealed clinically and many lymphocytes including large lymphocytes are observed on aspiration cytology.View full abstractDownload PDF (7153K) -
Imprint cytologic findings with special emphasis on the multinucleated giant cellsMinoru SHINOZAKI, Motoshige KUDO1993 Volume 32 Issue 4 Pages 562-566
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of juvenile xanthogranuloma arising in the skull of a 2-month-old female infant is reported. The patient had no history of trauma or hyperlipidemia. The xanthogranulomatous lesion was discrete and osteolytic, and involved the dura mater to some extent, sparing the skin and brain. It was composed of a mixture of multinucleated Touton and osteoclast giant cells, mononuclear fibrohistiocytes, and scattered lymphocytes and eosinophils. They were immunohistochemically negative for S-100 protein and ultrastructurally contained no Birbeck granules. As expected from the histologic findings, both types of giant cells were easily identified in the imprint cytologic preparation. They each had their own distinct cell configuration and nuclear shape, number and arrangement. The foamy cytoplasm of Touton giant cells was, however, equivocal in the cytologic specimen, which was thought to be either a possible artifactual destructive loss at the time of imprinting or to be Touton giant cells simply not containing lipids. Juvenile xanthogranuloma of bone is exceptional, and this is, to our knowledge, the first cytological report in the literatView full abstractDownload PDF (6885K) -
Shigeharu HATAKEYAMA, Chieko KUMAGAI, Kazuyo TAKEUCHI, Takashi MASUDA, ...1993 Volume 32 Issue 4 Pages 567-572
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of coccidioidomycosis detected in aspiration biopsy cytology of the neck is reported.
Our report aims to describe the morphological characteristics of Coccidioides immitis (C. immitis) spherules in cytologic preparations. The patient was a 35-year-old Japanese male who had returned from California, U. S. A.
Many spherules of C. immitis were observed in the purulent inflammatory background of the smear.
The spherules appeared orangeophilic to eosinophilic with Papanicolaou stain and were composed of a nonbudding spherical, thick-walled structure measuring 5 to μ jC in diameter. The spherules were round with double walls, and resembled pollen. Without awareness of coccidioidomycosis, therefore, they might be misidentified as pollen.
The mature spherules were filled with round, non-budding endospores, measuring 2 to 5μ in diameter. There were also a few free endospores in the smear. Some of the spherules exhibited the Splendore-Hoeppli effect, consisting of a hyaline, pale eosinophilic, radiating precipitate around the organisms.
Cytological examinations have been shown to be a useful tool for establishing the diagnosis.
Mycoses introduced into Japan from abroad have been increasing, making it appear that Japanese cytotechnologists and cytologists should know the morphological characteristics of C. immitis in the cytological material.View full abstractDownload PDF (8356K) -
Akira SAITO, Junichi KOYATSU, Kazuhisa ISHI, Sakae UTSUNO, Shu SAITO1993 Volume 32 Issue 4 Pages 573-574
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (2617K) -
Hiroo MOCHIZUKI, Takashi WATANABE, Makoto SUGIURA, Ichiro MORI, Hirosh ...1993 Volume 32 Issue 4 Pages 575-576
Published: 1993
Released on J-STAGE: November 08, 2011
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Satoshi TOSHIMA, Tadashi ARAI, Atsushi UCHIDA, Naoki YOSHIMI, Yo YASUD ...1993 Volume 32 Issue 4 Pages 577-578
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (1808K) -
Hiroshi KITAGAWA, Yasushi YATABE, Koji MATSUSHITA, Kazuho UCHIDA, Jun ...1993 Volume 32 Issue 4 Pages 579-580
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (4761K) -
A case reportReiko TAKADA, Fujie MIYAZAWA, Hiroko KASAHARA, Kenji MATSUZAWA, Jun NA ...1993 Volume 32 Issue 4 Pages 581-582
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (4071K) -
A case reportKumiko TANAKA, Atsuhiko SAKAMOTO, Masafumi TUZUKI, Kanako HOSAKA, Isam ...1993 Volume 32 Issue 4 Pages 583-584
Published: 1993
Released on J-STAGE: November 08, 2011
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Toshinari TAKAHASHI, Kazuhiro TAKAHASHI, Takashi SATOH, Nobuhiro MONMA1993 Volume 32 Issue 4 Pages 585-586
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (2543K) -
Tohru HARADA, Takashi NIKAIDO, Kazuhito NAKAMORI, Harumi SHIMIZU, Yuki ...1993 Volume 32 Issue 4 Pages 587-588
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (3162K) -
Junichi KOYATSU, Katsunari KINA, Reiko KAZAMA, Akira SAITO, Kazuhisa I ...1993 Volume 32 Issue 4 Pages 589-590
Published: 1993
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (4334K)
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