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Volume 35, Issue 1
Displaying 1-14 of 14 articles from this issue
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A study of their nuclear configurationYasuyuki MIYAKE, Noriko OSUGI, Takuo KANAHARA, Sakae HATA, Mitsuyoshi ...1996 Volume 35 Issue 1 Pages 1-5
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe examined 23 cases of ovarian epithelial tumors and studied their nuclear configurations on inprint cytology.
The tumors consisted of 10 mucinous adenomas, 3 mucinous adenocarcinomas, 3 serous adenomas, 6 serous adenocarcinomas, and 1 endometrioid carcinoma.
The incidences of coffee-bean nuclei and intranuclear cytoplasmic inclusions were 14.8% and 2.8% in mucinous adenoma, and 1.5% and 0.2% in the other surface epithlial tumors, respectively.
We conclude that coffee-been nuclei, intracytoplasmic inclusions and semilunar nuclei are nuclear features characteristic of ovarian mucinous adenoma, and are very useful markers for inprint cytology.View full abstractDownload PDF (6280K) -
Shoji ONO, Kazuo KASE, Takao OGATA, Yoshiro EBIHARA1996 Volume 35 Issue 1 Pages 6-13
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSUsing a silver staining technique, nucleolar organizer region-associated proteins (AgNORs) were studied in ten sputum specimens containing atypical squamous cells, five of early squamous cell carcinoma, and five of invasive squamous cell carcinoma.
The following results were obtained:
1) The mean number of AgNORs per nucleus was 2.43±0.3 (mean±SD) in atypical squamous cells, 3.29±0.3 in early squamous cell carcinoma, and 4.51±0.7 in invasive squamous cell carcinoma.
2) The mean diameter of AgNORs in the nucleus was 1.04±0.53μm (mean±SD) in atypical squamous cells, 1.48±0.65μm in early squamous cell carcinoma, and 2.14±0.87μm in invasive squamous cell carcinoma.
There were highly significant differences between the AgNORs numbers and diameters in atypical squamous cells, early squamous cell carcinoma, and invasive squamous cell carcinoma (P<0.005).
Furthermore, the differences in morphologic features of AgNORs were compared between the cells.
As a result,
3) The ratio of cells having more than 5 small black dots in the nucleolus was 15% in atypical squamous cells, 57% in early squamous cell carcinoma, and 78% in invasive squamous cell carcinoma.
4) The ratio of cells having numerous small black dots in the nucleus was 3% in early squamous cell carcinoma, and 10% in invasive squamous cell carcinoma, but 0% in atypical squamous cells.
These results suggest that AgNORs staining is a useful technique for the differential diagnosis of squamous cell atypias.View full abstractDownload PDF (9864K) -
Junko MARUTA, Shiro NOGUCHI, Hiroto YAMASHITA1996 Volume 35 Issue 1 Pages 14-19
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSIn the past 24 years, we have experienced 17 cases of adenolipoma in 33766 thyroid glands removed during surgery. The sex ratio was 16: 1 (F: M) and the mean age was 59 years old. No patient showed clinical signs of hyperthyroidism or hypothyroidism. Ultrasonography revealed a focal nodule, 131I scintigraphy a cold nodule. Sliced sections revealed a well-circumscribed, yellow, solid mass, with an average tumor diameter of 2.5 cm. On histological examination, follicular epithelium had trabecular, microfollicular, and macrofollicular structures admixed with mature adipose tissue. The follicular epithelium was immunohistostained with anti-thyroglobulin antibody and the adipose tissue was stained with Sudan III. Amyloid staining was negative. Four of the 17 cases had simple adenolipoma and the others were complicated mostly by adenomatous goiter. Cytological examination showed follicular epithelial clusters without morphological diversity and scant adiopse tissue. When mature adiopse tissue is observed by fine-needle aspiration biopsy, adenolipoma should be considered.View full abstractDownload PDF (6409K) -
Comparison of mucocele-like tumor and mucinous carcinomaTakashi KITAMURA, Toshiyuki MITSUYA, Kazuya YAMASHITA, Takeshi NAKAMUR ...1996 Volume 35 Issue 1 Pages 20-25
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA comparative study of CA 15-3 localization was made in 2 cases of Mucocele-like Tumor (MLT) of the breast and 9 cases of Mucinous Carcinoma (MC), using needle aspiration cytology. In the MLT cases, a sheet of small cells and three-dimensional cell populations were observed on the mucocele. In the MC cases, 4 types were identified on the basis of idioblasts and morphological appearances. From comparison of MLT and MC, the MC having small nuclei resembling those of MLT also had a cell component on the Mucocele-like MLT; moreover, MC cell uptake was abundant. The tumor cells had nucleoli in a nucleus slightly larger than that of the MLT. The two were morphologically distinguishable. CA 15-3 was negative in some MC, but there was a recognizable difference in localization in the other MC and MLT cases.View full abstractDownload PDF (8210K) -
Takako TAKEUCHI, Hiroaki TAKEUCHI, Lili KOU, Hisaaki SHINOHARA, Takako ...1996 Volume 35 Issue 1 Pages 26-31
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSMore than 90% of adults have evidence of pastinfection with human cytomegalovirus (HCMV).HCMV is also an important causative agent of virus reactivated disorders in immunosuppressed patients including those with AIDS and those who have undergone transplantation.
In order to acheive highly sensitive detection of HCMV infection in cytopathologic examination with the Papanicolaou stain, using HEL cells (human embryo lung fibroblasts) which are permissive for HCMV infection, or in vitro transfection assay was carried out. At each postinfection point, i. e. non-infected (0 hour postinfection ; 0 hpi), 6 hpi, 12 hpi, 24 hpi, 48 hpi and 72 hpi, cell smears and cell blocks (paraffin sections) were prepared, stained and observed in detail.
The results obtained indicate that striking cellular aggregation leading to conformation with various sizes of clusters had occurred by six hours postinfection and that multinucleated cells and giant cells gradually appeared without clear nuclear molding or a ground glass appearance. Increasing nuclear size was evident and numbers of small halos gradually appeared in the cytoplasm and/or nucleus with or without inclusion bodies. Futhermore, anisocytosis, cell rounding formation and conspicuous inclusion bodies were observed, while these clusters showed a tendency to be isolated. At 48 hpi, a few cells with the typical owl's eye were observed and the number of such cells had increased by 72 hpi. HCMV infection of these cells was confirmed with an FITC (fluorescin isothiocyanate) labeled probe using the FISH (fluorescin isothiocyanate in situ hybridization) method.View full abstractDownload PDF (6936K) -
A basic study on these methods and their clinical applicationKatsunari KINA, Kazuhisa ISHI, Tetsuya OKAZAKI, Reiko KAZAMA, Junichi ...1996 Volume 35 Issue 1 Pages 32-37
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSResponse conditions of cultured cells incorporating HPV-DNA to PCR-ISH and in situ PCR, which are sensitization methods for cell and tissue sections, were investigated, and the following results were obtained.:
1) As the response to PCR, the most consistent feature was observed at 20 cycles, which included denaturing at 90°C for one minute, annealing at 55°C for 2 minutes, and extension at 72°C for 3 minutes.
2) With each method of PCR-ISH and in situ PCR, DNA could be detected in cells fixed in 95% ethanol, as routinely used for cytodiagnosis.
3) A positive finding was observed in the nuclei of cultured SiHa cells with one copy of HPV-DNA, detection of which has been believed to be impossible with the ISH method alone, by applying PCR-ISH and in situ PCR. Our results suggest that these methods are applicable to the detection of viral.
DNA and oncogenes in clinical cytodiagnosis specimens, based on these methods overcoming certain disadvantages, i. e., the low sensitivity of the ISH method. Furthermore, DNA localization could be morphologically confirmed. The reliability of DNA diagnosis was also considered to increase in that contamination, a problem with the PCR method, can be morphologically detected by these methods.View full abstractDownload PDF (4631K) -
Takayuki HIRANO, Hiroshi TANAKA, Syunichi YOSITAKE, Hisato MASUMOTO, T ...1996 Volume 35 Issue 1 Pages 38-43
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA very rare case of simultaneous primary bilateral breast cancer, the right side diagnosed as secretory carcinoma and the left as papillotubular carcinoma, is presented.
A 33-year-old woman was admitted to the Saga Prefectural Hospital because of a bloody discharge from the left nipple. Fine needle. Aspiration Cytology (FAC) of the left tumor showed findings of infiltrating duct carcinoma. A tumor of the right breast was noticed by chance, but FAC was negative. Intraoperative pathological findings of the right tumor revealed a secretory carcinoma. The right tumor was located in the E-portion, being 2.3×1.7×2.0 cm in diameter, and was accompanied by axillary lymph node metastasis.
Histopathologically, the tumor was composed of papillary or solid nests and a characteristic microcystic growth pattern was evident in the multinodular foci. The tumor cells had clear cytoplasms and microscopic spaces contained abundant mucoid materials, which were positive for the PAS reaction. Electron microscopy showed secretory vacuoles within the cytoplasms of the tumor cells and in the intracytoplasmic lumina.
In print smears, the tumor cells had abundant, pale to clear cytoplasm with small, round, cytologically low-grade nuclei. Mucous globular structures (MGS) and bunch of grapes structures were among the characteristic features, and intracytoplasmic luminal spaces were also diagnostic.View full abstractDownload PDF (10119K) -
Akira YUKIMASA, Mayumi KUROSU, Yousuke KINOSHITA, Katsuaki MATSUI, Osa ...1996 Volume 35 Issue 1 Pages 44-48
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThe patient was a 43-year-old female. A tumor was recognized at the hepatic hilus, and probe-laparotomy was done. Cells of two types appeared in the necrotic background of bile collected intraoperatively. In one type, the cells were few and degenerative in the bile duct and had pale cytoplasm, maldistributed round nuclei and obvious nucleoli. In the other type, the cells were small and round with denuded nuclei. These cells were scattered or conglomerated, and showed a partly indian file formation. Based on the findings of these cells, we considered a double malignancy, and the former was diagnosed as adenocarcinoma, the latter as a malignant lymphoma. Furthermore, an apparent connection between the latter was considered to reflect an epithelial component. We therefore diagnosed an undifferentiated carcinoma. On histopathological examination after autopsy, adenocarcinoma was not recognized in the bile ducts or other areas. This case was thus diagnosed as an adult type of neuroblastoma (NB).
Immunohistochemically, the tissues of this tumor stained positive with NSE, synaptophysin and Goα. On reexamination, the cells in the bile that had been considered to be adenocarcinoma, were re-diagnosed as glandular atypical cells, because nuclear chromatin was equally distributed and no increase was seen.
Small round cells with denuded nuclei seen in the bile had characteristic findings of NB cells. We considered these to be adenocarcinoma, however regarding the degenerative bile duct cells. We did not consider NB in this patient because of her age at presentation. Thus, in making a cytological diagnosis in must be kept in mind that there are adult NB cases.View full abstractDownload PDF (9004K) -
Mitsuyoshi HIROKAWA, Michio SHIMIZU, Takashi FUKUYA, Yasuto HIGASHI1996 Volume 35 Issue 1 Pages 49-50
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
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Kazuaki KIYOYAMA, Tadanobu KURIBAYASHI, Kazumi KOGA, Eiko MINEMATSU, H ...1996 Volume 35 Issue 1 Pages 51-52
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
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Hirofumi NAKAYAMA, Chitoshi HIDAKA, Keiko MURAKAMI, Ayano AOKI, Wataru ...1996 Volume 35 Issue 1 Pages 53-54
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
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Tomomi HASUMOTO, Hiroshi OBA, Suguru TORII, Eio ATARI1996 Volume 35 Issue 1 Pages 55-56
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
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Takashi ITOH, Takayuki MORITA, Mitsuru KONN, Yoshimasa KAMATA, Norito ...1996 Volume 35 Issue 1 Pages 57-58
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
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Reiji HABA, Shoji KOBAYASHI, Katuyuki NOMA, Yoshihito YANO, Masakiti U ...1996 Volume 35 Issue 1 Pages 59-60
Published: January 22, 1996
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (4227K)
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