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Volume 36, Issue 5
Displaying 1-16 of 16 articles from this issue
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Katsunari KINA, Kazuhisa ISHI, Naoko OKUYAMA, Tetsuya OKAZAKI, Reiko K ...1997 Volume 36 Issue 5 Pages 473-477
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe performed the in situ polymerase chain reaction (PCR) on archival cervical smears (demounted Papanicolaou-stained smears) from 27 patients, in which human papilloma virus (HPV)-DNA had been detected by southern blot hybridization or dot blot hybridization but not by in situ hybridization (ISH), and on the corresponding biopsy specimens collected at the same time.
We obtained the following results:
1. HPV-DNA was detected in the archival cervical smears by in situ PCR in 6 of the 27 cases (59.3%). The rate of detection HPVpU-31 B was 60.0%(3 of 5 cases) for types 6 and 11; and that of HPVpU-1 M was 50.0%(7 of 14 cases) for types 16 and 18 and 75.0%(6 of 8 cases) for types 31, 33 and 35.
2. HPV-DNA was detected in a dispersed pattern inside the nuclei mainly in atypical cells and in some normal cells. Positive signals were detected in normal cells in 1 case each of cervical intraepithelial neoplasia 1 and 2.
3. HPV-DNA was detected in 20 of the 27 biopsy specimens (74.1%). This finding agreed with that from the archival cervical smear in 24 of the 27 cases (88.9%), and disagreed in 3 of them (11.1%).
Our results show that the in situ PCR can be applied to archival cytologic specimens. With sensitivity superior to that of ISH, this method will contribute greatly to genetic analysis of cellular morphology in the future.View full abstractDownload PDF (2915K) -
Hiroki NAKAYAMA, Daisuke DOI, Ryo ONOSE, Naoki KAWAI, Hisamori KATO, H ...1997 Volume 36 Issue 5 Pages 478-483
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe assessed the slides and investigated how to use the cytology in 383 cases of cervical dysplasia and cancer at the first clinic for clarifying the problems in the cytology in screening for cervical cancer. The results are as follows.
1) Cytology was performed in 93.7% of all cases.
2) A cotton swab was used in 65.1%, and a “cytopick” in 34.9%.
3) Two slides were used in 68.8%.
4) 27.2% of the slides offered by the first clinic were unsatisfactory for evaluation.
5) Classification of the cytology suggested the right pathological diagnosis in 47.4% and screening by cytology tended toward underdiagnosis.
6) 36.4% of the slides offered by the first clinic were underdiagnosed by our re-observation.
7) Cervical cancer was found in 26% of the cases classed cytologically as type Ilia. These cases had to be examined at a higher level, so 16.5% of them were followed up only with cytology.
We believe that the clinician must make an effort to become skillful in picking up and establishing and maintaing the process of examination for the purpose of advancing cytologyView full abstractDownload PDF (753K) -
Ritsuko IMAI, Sonoko NATSUME, Tatsunari SATAKE, Toshiaki FUKATSU1997 Volume 36 Issue 5 Pages 484-489
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDuring screening of endometrial smears, we occasionally encounter benign but atypical columnar cell clusters (benign atypical cells). In this study, we determined the histological features corresponding to benign atypical cells. We used both 100 endometrial smears containing benign atypical cells and 100 cell block preparations which were made of the same endometrial tissues obtained by the cytology endocyte method. The benign atypical cells and their corresponding histological features were classified into 3 types. Type 1: Columnar cells forming rather thick clusters, some of them with opaque cytoplasm, variable sized nuclei and visible nucleoli. Histologically, these cells correspond to those in the endometrial surface epithelium showing a papillary or flat pattern. Type 2: Large round, polygonal or fibrous cells with prominent nucleoli and abundant opaque cytoplasm. These cells usually are arranged in a sheet and histologically correspond to those in the so-called regenerating endometrial surface epithelium. Type 3: Columnar cells usually forming thin clusters and having transparent cytoplasm. Nuclei of these cells are usually larger than those of type 1. Histologically, these cells correspond to those showing various atypicality in the normal endometrial glandular epithelium. It is essential to differentiate type 1 cells from well-differentiated endometrial adenocarcinoma cells. For that purpose, it is important to take notice of both the multilayered structure of cell clusters and the short diameter of the cells composing such clusters.View full abstractDownload PDF (9353K) -
Masami SATO, Yasuki SAITO, Takaichiro SUZUKI, Shin SEKIMOTO, Hiroshi I ...1997 Volume 36 Issue 5 Pages 490-497
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTo examine the diagnostic criteria of sputum cytology for lung cancer mass screening in Japan, we sent photographs of sputum cytology to institutes (including laboratories and hospitals) which were involed in diagnosis of respiratory cytology. Diagnoses were obtained from each. As a result, for patients with typical malignant cells, further examinations including bronchoscopy were recomended for all institutes. However, in patients, with borderline atypical squamous, cells having early lung cancer or having dysplasia, diagnosis differed with the institute.
To justify the diagnostic criteria of sputum cytology, re -evaluation of sputum in patients before lung cancer was detected was recommended for each institute. This would lead to early detection of both lung cancer and precancerous lesions in mass screeing.View full abstractDownload PDF (6632K) -
Midori ONO, Yoshio SHIINA, Jun KANEMOTO, Teruko KOMODA, Hisaichi BANNA ...1997 Volume 36 Issue 5 Pages 498-505
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSMorphological analysis of intracytoplasmic inclusion bodies in human line (HL) cells inoculated with Chlamydia pneumoniae was carried out to examine the possibility of cytological diagnosis. The inclusions were hematoxylinophilic on Papanicolaou staining and occurred close to the nuclear rim. Although there was generally only a single inclusion in each infected cell, the number increased when HL cells were inoculated with abundant C. pneumoniae. The size of each inclusion increased step by step, reaching 6μm in diameter, and finally becoming very large as a result of fusion. Some nuclei appeared to be partly compressed by the inclusion, but signet ring formation was not observed. The margin of the inclusion was generally indistinct, and there was an unstained gap between the inclusion and the cytoplasm. The inclusions were mostly round except for those that were in the process of breakdown. From these results, we conclude that detection of chlamydial inclusions is possible on Papanicolaou-stained preparations of C. trachomatis infections in the genital tract. However, practical consideration needs to be given to mild morphological changes due to differences in the host cell, and the low rate of occurrence of inclusion-bearing cells.View full abstractDownload PDF (7701K) -
Kumiko NAKAZAWA, Tsutomu YUMINAMOCHI, Yoshio ISHII, Naomi HAYAKAWA, Ry ...1997 Volume 36 Issue 5 Pages 506-511
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSImmunocytochemical staining for p53 was evaluated in 132 cases of effusion. In mesothelial cells of patients without malignancy. p53 protein was not detected in 4 out of 20 patients (20%). In 15 patients (75%), less than 10% of the cells examined were positive for p53 (1+). One patient (5%) was strongly positive for p53 (2+, 11-50%). Gene analysis revealed that the expression of p53 on these mesothelial cells was of a wild type. p53 expression in malignant cells was detected in 88 of 112 patients (78.6%). In 15 patients, the number of positive cells was less than 10%(1+). Forty of the 112 patients (36%) had positive cells ranging from 11 to 50%(2+). In 33 patients (29%), the rate of positive cells was higher than 51%(3+). That p53 expression with over 2+ staining was detected in 65% of the malignant cells suggests that immunocytochemical staining for p53 in effusion is useful for cytological diagnosis. In terms of origin, pancreas carcinoma (89%), endometrial adenocarcinoma (88%), small cell carcinoma of the lung (75%), and adenocarcinoma of the Iung (70%) had an especially high rate of p53 expression. There appears to be no correlation between the rate of p53 expression and prognosis.View full abstractDownload PDF (2672K) -
Shigeru HASHIMOTO, Naoyuki KASAI, Ayako SUZUKI, Nobutaka SUZUKI, Makio ...1997 Volume 36 Issue 5 Pages 512-516
Published: September 22, 1997
Released on J-STAGE: December 05, 2011
JOURNAL FREE ACCESSIn spite of recent advances in the management of ovarian malignancy, the prognosis for ovarian malignant Müllerian mixed tumor is extremely poor. Therefore preoperative information is essential for successful treatment of the aggressive tumor. We used immunohistochemistry preoperatively for the cytodiagnosis of ascitic cells. The 69-year-old patient exhibited rapidly increasing abdominal fullness, constipation, and elevated tumor markers. Peritoneal effusion cytology specimens were diagnosed as carcinosarcoma having both adenocarcinomatous and sarcomatous components. The cytomorphologic features helpful in the recognition of a mesenchymal component included a dissociated smear pattern of pleomorphic round to oval cells and/or spindle cells having high nuclear to cytoplasmic ratios. The carcinomatous cells were positive for both CA-125 and BerEP 4, and negative for vimentin. The mesenchymal cells were negative for both CA-125 BerEP 4, and positive for vimentin At laparatomy, the dominantly enlarged right ovary seemed to be the primary lesion, and metastasis into the omentum and the subphrenic area was observed. The right ovary was removed and postoperative combination chemotherapy was administered. Double platinum chemotherapy was very effective for this patient. The levels of CA-125 were normalized after this therapy. A second cytoreductive operoation was carried out and postoperative combination chemother apy was administered. She remains free of disease 42 months after the initial therapy. The pathological diagnosis was a malignant Müllerian mixed tumor of the ovary. The epithelial elements were moderately differentiated adenocarcinoma and the mesenchymal elements were stromal sarcoma. The immunocytochemical staining by vimentin, BerEP 4, and CA-125 was very useful in the recognition of the mesenchymal component and the carcinomatous component of Müllerian mixed tumor in the cytological samples.View full abstractDownload PDF (3816K) -
Report of a caseMasahide ARAI, Toshiko JOBO, Rinya SATO, Jun WATANABE, Hiroaki IWAYA, ...1997 Volume 36 Issue 5 Pages 517-520
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of stromal Leydig cell tumor with prominent crystalloids of Reinke is presented. In pathological specimens, the tumor arose in a background of ovarian stromal cell proliferation with foci of Leydig cells. The stromal component of the tumor resembled fibroma and Leydig cells had abundant eosinophilic cytoplasm and prominent Reinke crystalloids. A small lesion of mucinous cystadenoma coexisted with this tumor. Imprint cytology of the tumor revealed 3 types of the tumor cells. I. Spindle-shaped cells with scanty cytoplasm forming loose clusters, 2. Cells with abundant light green-stained cytoplasm and oval nuclei which consisted of stratified clusters, 3. Cells showing a palisading pattern. Rod-shaped and orange-G stained crystalloids (crystalloids of Reinke) were observed frequently within cellular clusters of the 2 types.View full abstractDownload PDF (6755K) -
Kei UEDA, Katsuhiro TERAMOTO, Yoshihiko OHKUBO, Hitoshi HARA, Masahiro ...1997 Volume 36 Issue 5 Pages 521-525
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe report a case of choriocarcinoma of the fallopian tube which is exceedingly rare.
A 49-year-old woman with her last pregnancy at age 31 years and menopause at age 47 years was referred to our hospital because of lower abdominal pain. A right adnexal tumor was suspected by findings from CT and echography. Endometrial aspiration cytology revealed Arias-Stella reaction, which indicated some trophoblastic disease. So we performed a total abdominal hysterectomy with bilateral salpingo-oophorectomy. The tumor measuring 7×4×4cm in the right fallopian tube was a necrotic and hemorrhagic mass. Histopathologic examination showed atypical trophoblastic cells with pleomorphic, hyperchromatic and multiple nuclei. Tumor cells were noted between the myosalpingeal fibers. Tumor emboli were observed within blood vessels in the tubal wall. No villi were present. The endometrium showed a characteristic Arias-Stella reaction, but no villi. These results lead us to conclude that this is a primary choriocarcinoma of the fallopian tube. Accordingly, it is suggested that aspiration cytology is useful tool to diagnose tubal trophoblastic disease.View full abstractDownload PDF (9603K) -
Takahisa TSUKAMOTO, Yositaka OHTA, Sonoe ITOH, Yuko NAKANO, Keiko OHTA ...1997 Volume 36 Issue 5 Pages 526-530
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSTwo cases of malignant lymphoma in the breast are reported with an immunocytochemical study. The two patients, a 78-year-old woman (case 1) and a 44-year-old woman (case 2) were admitted to the hospital because of breast tumors. Aspiration cytology revealed tumor cells of a size similar to that of neutrophils, having round to oval nuclei and high N/C ratios and some them having cleaved nuclei. May-Giemsa staining showed basophilic cytoplasm and fine nuclear chromatin suggesting malignant lymphoma, but it was necessary to exclude poorly differentiated adenocarcinoma.
Immunocytochemical studies using various antibodies (LCA, L -26, UCHL -1) were performed. In case 1 the antibodies were applied on the lavage smear with a syringe, and in case 2 on a dried aspiration smear after decoloration of Papanicolaou stain. Immunocytochemically, case 1 was immunoreactive with LCA and L 26, and case 2 was positive for LCA on the surface of the tumor cells. The results suggested malignant lymphoma. The histopathological diagnoses of the resected tumors were non-Hodgkin's lymphoma, diffuse and large cell type, in case 1 and non-Hodgkin's lymphoma, diffuse and medium-sized cell type, in case 2. The patients received chemotherapy.View full abstractDownload PDF (9201K) -
Acase reportTakako KANEKO, Mamoru MOCHIZUKI, Hando HAKOZAKI, Sakiko IGARI1997 Volume 36 Issue 5 Pages 531-536
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe herein describe a case of primary gastric choriocacinoma examined by imprint cytology.
A 58-year-old Japanese man visited our hospital because of tarry stools. Upper gastrointestinal endoscopy revealed a Borrman's type 2 lesion in the cardia of the stomach. The patient underwent total gastrectomy, patial pancreatectomy and splenectomy.
The imprint materials obtained from the tumor showed both a loosely packed clump of cells and groups of isolated cells. Blood and a necrotic substance were conspicuous in the materials. Three types of malignant tumor cells wereidentified as follows: 1) abnormal mononucleated cytotrophoblastic cells possessing large round nuclei, granular chromatin, prominent nucleoli, and scanty and thin cytoplasm; 2) abnormal multinucleated syncytiotrophoblastic cells possessing multiple enlarged nuclei and abundant eosinophilic cytoplasm; 3) abnormal mononucleated intermediate trophoblastic cells, possessing enlarged and irregular-shaped nuclei, and moderately abundant cytoplasm.
We believe that the following features on imprint cytology helped to differentiate gastric choriocarcinoma from other primary gastric malignancies, such as poorly differentiated carcinoma, malignant melanoma and malignant lymphoma: 1) eqithelial arrangement of cells, 2) fine and thin cytoplasm 3) identification of three types of neoplastic cells consisting of cytotrophoblastic, syncytiotrophoblatic and intermediate trophoblastic cells.View full abstractDownload PDF (10463K) -
Takuo KANAHARA, Mitsuyoshi HIROKAWA, Kanae ARIMITSU, Mari NARITOMI, Sa ...1997 Volume 36 Issue 5 Pages 537-540
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSAspiration cytology of tumoral calcinosis-like lesions in two patients undergoing hemodialysis is described. The patients were a 53-year-old man and a 57-year-old man. Fine-needle aspirations from the tumors revealed a large number of crystals. Most of the crystals were round and showed concentric figures. Their inner areas were granular and yellowish while their external surfaces were brilliant and pale green with a layered structure. They were not birefringent by polarized microscopy. Histiocytes and osteoclastic type multinuclated giant cells were seen in one case. We consider that these crystals were helpful for diagnosing these cases cytologically as tumoral calcinosis-like lesions.View full abstractDownload PDF (6842K) -
Raj K Gupta, SN Rao, Gary Picken1997 Volume 36 Issue 5 Pages 541-544
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSNeedle aspiration cytology of an unsuspected right sided hip mass was performed in a 46 year old female of Indian origin. Despite the lack of any proven diagnosis or any improvement in her symptoms of constant pain and restricted movement, she was treated with antituberculous drugs. The cytologic samples of the aspirate were cellular and featured clusters of uniform ductal epithelial cells with cystic spaces, bland nuclei, fine chromatin and scanty cytoplasm arranged around cores of homogenous material or spheres of metachromatic material. The cytologic diagnosis, on site, was reported as a metastatic adenoid cystic carcinoma. This was further substantiated with a cell block of the aspirate and trucut biopsy. Later, it was revealed that about 13 years earlier she had had a submandibular salivary gland mass removed and this was diagnosed as an adenoid cystic carcinoma. This case is of interest, since the needle aspiration cytodiagnosis suggested an on site unequivocal diagnosis in an otherwise undiagnosed hip mass.View full abstractDownload PDF (6063K) -
Hisatoyo KASAI, Katsuhiko MAEDA, Masako ITO, Hiroshi NAKANO, Akira KAM ...1997 Volume 36 Issue 5 Pages 545-549
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe report a case of angiosarcoma of the neck. A 71-year-old woman noticed a tumor mass on the left side of her neck and was admitted to our hospital. Fine needle aspiration was performed. A dark-redish fluid was obtained. The aspiration smear showed many clusters in a hemorrhagic and inflammatory background. The tumor cells showed small clusters and fast connection. Rosette-like formations were also present. The tumor cells had round or oval nuclei with a high N/C ratio and nuclear glovement. According to immunohistochemical examination of resected specimen, the tumor cells were positive for factor VIII and CD 31. Weibel-Palade bodies were demonstrated in the cytoplasm of the tumor cells by electronmicroscopy. Angiosarcoma shows various cytologic findings and is difficult to diagnosis cytologically. However, angiosarcoma was suspected from the condition of the fluid and the appearence of the cells. Immunocytochemical and electronmicroscopic studies were necessary for differential diagnosis.View full abstractDownload PDF (6003K) -
Masashi SHIMODA, Natsuko TOJI, Minoru MATSUDA, Hiroshi UENO, Tokio NIS ...1997 Volume 36 Issue 5 Pages 550-551
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (3048K) -
Yuri OZEKI, Yoshiaki NORIMATSU, Masumi FURUTANI, Takeshi OKINO, Chouta ...1997 Volume 36 Issue 5 Pages 552-553
Published: September 22, 1997
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (5016K)
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