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Volume 38, Issue 5
Displaying 1-22 of 22 articles from this issue
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application to early detection of corpus cancerYuko SUGIYAMA, Yasuo HIRAI, Atsuko MINAMI, Masafumi TSUZUKU, Katsuhiko ...1999 Volume 38 Issue 5 Pages 379-384
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSObjective: To detect early endometrial cancer, we identified the cytologic features of small endometrial cancers with a diameter of less than 1.0cm.
Study Design: From 1986 to 1992, 367 patients with endometrial cancer underwent an operation. We reviewed 46 small endometrial cancers cytologically and histologically. For endometrial cytology, aspiration smears were performed with a polyethylene tube and syringe. Histologically, endometrial biopsies and operative materials were examined. The entire endometrium was examined by step sections. Results: The positive endometrial cytology rate was 70%. There were 10 false-negative cases. Review of these false-negative cases found cytologically atypical syncytial change in 6 cases. Atypical syncytial change was a characteristic cytologic finding, with papillary structure of the endometrium with syncytial cytoplasm, mild nuclear atypia and inclusion of neutrophils in the cluster.
Conclusion: If we find atypical syncytial changes in endometrial cytology, it is important not to miss the early endometrial cancer.View full abstractDownload PDF (8046K) -
Midori ONO, Yoshio SHIINA, Yukari ABE, Mineo OOMURA, Mitugu FUKUOKA1999 Volume 38 Issue 5 Pages 385-392
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSObjective: Angiogenesis essential for the proliferation of tumor cells. We observed the morphological features of endothelial cells and the pattern of vessels in cytologic preparations of endometrium stained by the Papanicolaou method. Study Design: The specimens comprised 15 samples of normal endometrium, 10 cases of endometrial hyperplasia and 40 cases of endometrial carcinoma. We determined the nuclear size and morphological features of endothelial cells, and measured the width of main vessels.
Results: The nuclei of endothelial cells were elliptical as well as round and tear-drop shaped in parts showing tortuosity or anastomosis, whereas they were generally elliptical in parts of vessels that were straight. Although nuclear chromatin was generally fine and evenly distributed in the endothelial cells, the amount of chromatin and the number of nucleoli tended to be higher in cancer cases than in normal or hyperplastic endometrium. With regard to distribution, fork-like structures that appeared to be new vessels, were observed at the ends of arborescent clusters in well differentiated adenocarcinoma.
Conclusion: On the basis of these findings, we conclude that cytomorphological observation of endothelial cells and vessels in cytological preparations may be useful for the differential diagnosis of endometrial adenocarcinomas.View full abstractDownload PDF (10136K) -
Masanobu ANAMI, Tomayoshi HAYASHI, Noriko SATO, Masachika ISEKI, Naoe ...1999 Volume 38 Issue 5 Pages 393-402
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSObjective: Salivary glands are suitable for cytodiagnostic examination because they are easily accessible. We assessed the value of cytodiagnostic examination in predicting the histologic diagnosis.
Study Design: Cytodiagnosis of salivary gland tumors reviewed from cases registered between 1990 to 1996 was compared with the final pathologic diagnosis.
Results: The cases examined included pleomorphic adenomas, Warthin's tumors, myoepithelioma, squamous cell carcinoma, mucoepidermoid carcinomas, adenocarcinoma, adenoid cystic carcinomas, acinic cell carcinoma and lowgrade polymorphous adenocarcinomas. The important features for a correct cytodiagnosis of salivary gland tumors included background findings, cytoplasmic granules, stromal cells, two-cell pattern (epithelial cells and lymph cells), and the specific histologic type of malignant cells.
Conclusion: Cytodiagnosis of salivary gland tumors was very useful, although it was difficult to establish the correct diagnosis when the specimen contained an insufficient amount of cells or when it showed incomplete features in cases not consisting of monotonous histologic features. Intraoperative cytodiagnosis was also useful when the frozen tissue sections were of poor quality due to the freezing process. Our results indicate that the combined use of both histological and cytodiagnostic examination provides a better diagnostic tool, compensating for the defficiencies of each method.View full abstractDownload PDF (4204K) -
Yuri TAMADA, Mitsuyoshi HIROKAWA, Yoshiaki NORIMATSU, Hiromi KHODA, Ta ...1999 Volume 38 Issue 5 Pages 403-407
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSObjective: Pleomorphic adenoma of the salivary gland is a benign tumor composed of epithelial, myoepithelial and mesenchymal elements. The myoepithelial cells show a variety of shapes, such as spindle, stellate, epitheliold and plasmacytoid (hyaline cells) or clear cytoplasm. To evaluate the morphology of the myoepthelial cells seen in pleomorphic adenoma, we examined seven cases of pleomorphic adenoma by immunocytochemistry.
Study design: The study group consisted of seven samples of pleomorphic adenoma obtained by fine needle aspiration cytology in Kurashiki Central Hospital. Immunocytochemical staining for vimentin, alpha-smooth muscle actin (α-SMA), S-100 protein, keratin 8, carcinoembryonic antigen (CEA) and epithelial membrane antigen (EMA) was performed on Papanicolaou-stained smears using the streptavidin-biotin method. We indentified vimentin-, α-SMA-, S-100 protein-and keratin 8-positive and CEA and EMA-negative cells as myoepithelial cells.
Results: Myoeithelial cells were subdivided into five types: plasmacytoid, lymphoid, tail, stellate and spindle cells. All types were observed in each case. Plasmacytoid cells were individually scattered. Lymphoid cells were located around or above cell clusters. Tail cells appeared at the periphery of cell clusters or around them. Stellate cells were present in mucoid material. Spindle cells formed bundles or were individually scattered.
Conclusion: Our results indicate that myoepithelial cells seen in pleomorphic adenoma morphologically showed five types, and all types were present in each case.View full abstractDownload PDF (6764K) -
Kiminari KAWANO, Haruhiko MIYAYAMA, Reiki NISHIMURA, Toshio BABA, Kouj ...1999 Volume 38 Issue 5 Pages 408-415
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSObjective: To determine cytological differences between early recurrent cases and nonrecurrent breast cancer.
Study Design: Fifty-eight FNAs cases were studied. They were divided into 4 groups; 7 node-negative and early recurrent cases within two years (group 1), 16 node-negative and cases nonrecurrent for more than five years (group 2), 15 node-positive and cases early recurrent within two years (group 3), and 20 node-positive and cases nonrecurrent for more than five years (group 4). Cytological atypia of the primary tumor was analyzed using an Olympus Micrometer VM-30.
Results: Early recurrent cases (groups 1, 3) showed significantly increased nuclear long axis length, nuclear shortaxis length, nuclear area, nucleolar long axis length, nucleolar short axis length, nucleolar area, incidence of nucleoli>2.5 μm and mitotic index than nonrecurrent cases (groups 2, 4). Our scoring system composed of these parameters, showed a significant difference between recurrent and nonrecurrent cases if the cutoff score was six.
Conclusion: More severe cytological atypia in FNAs of breast cancer was seen in early recurrent patients independent of nodal status. Our scoring system of cytological dysplasia should be a useful parameter of early recurrence in breast cancer.View full abstractDownload PDF (4939K) -
Yusuke MATSUURA, Yoshiko KASHIMURA, Toru KIKUTSUGU, Shinichiro TOKUFUJ ...1999 Volume 38 Issue 5 Pages 416-420
Published: September 22, 1999
Released on J-STAGE: December 05, 2011
JOURNAL FREE ACCESSBackground: Uterine carcinosarcoma is a rare and fatal disease. It is difficult to make a correct cytologic diagnosis before treatment.
Case Report: We report the cytologic and pathologic findings in 3 cases of carcinosarcoma. Positive cervical cytology was noted in only 1 case. Positive endometrial smears were seen in all 3 cases. Both epithelial and nonepithelial malignant cells were seen in only 1 case, and epithelial or non-epithelial malignant cells were noted in one case each. Correct cytologic or pathologic diagnosis was made in only 1 of 3 cases before treatment. Imprint smear from the uterine specimen showed both epithelial and non-epithelial malignant cells. Immunohistochemically, conflicting results were obtained in the histopathologic specimen; some of the non-epithelial malignant cells showed positive staining for an epithelial marker.
Conclusion: The cytologic diagnosis of carcinosarcoma of the uterine body should be made carefully.View full abstractDownload PDF (8587K) -
The usefulness of measuring mitotic figures on the imprint smearTakeshi FUJISAWA, Eiji OHNO, Manabu HATTORI, Masaru YOKOYAMA, Jun WATA ...1999 Volume 38 Issue 5 Pages 421-426
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: We experienced a rare case of high grade, endomentrial stromal sarcoma (ESS), and tried to determine whether it is possible to estimate the grade of ESS by using the imprint smear.
Case: The patient was a 65-year-old woman with the chief complaints of genital bleeding and lower abdominal pain. Although she was operated on with a possible diagno sis of uterine malignancy, she underwent exploratory laparotomy.laparotomy. The cytopathological findings included;(1) large number of cells similar to normal endometrial cells, (2) small -to medium-sized uniform cells, (3) fine granular chromatin, pleomorphic nuclei with indentation, and (5) no specific differentiation of the cells and (6) frequent mitotic figures. Incidence of the mitotic figures was 0.42%(76±8.0/10 HPF) in pathological specimens, and 0.49%(19±0.0/10 HPF) in cytological specimens.
Conclusion: The presence of mitotic figures on imprint smears may be a useful means to assess the grade of ESS.View full abstractDownload PDF (7921K) -
Atsushi SUZUKI, Kazuya MAKITA, Minatsu MIYASAKA, Hirotaka ISHIMORI, Ei ...1999 Volume 38 Issue 5 Pages 427-430
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Uterine carcinosarcomas are rare neoplasms with a poor clinical prognosis often misdiagnosed prior to surgery. We report a case of uterine corporeal carcinosarcoma diagnosed preoperatively.
Case: The patient was a 62 year-old female with an initial complaint of lower abdominal pain. She presented with a large necrotic, pedunculated polypoid mass protruding from the endometrial cavity ; however, both cervical and endometrial cytology were negative and endometrial biopsy specimens presented with only necrotic tissue. The tumor was expelled from the vaginal cavity following hospitalization; a histopathological diagnosis of the tumor revealed carcinosarcoma. Surgery and postoperative chemotherapy were carried out but the patient died 7 months later due to disease progression. The surgical specimen presented with osteosarcoma components and the final postoperative diagnosis was confirmed to be carcinosarcoma with heterologous components.
Imprint cytology of the tumor revealed two differing cellular components: adenocarcinoma cells and undifferentiated malignant tumor cells. The adenocarcinoma cells appeared in three-dimensional aggregates ; the cells had increased N/C ratio, nuclei with fine granular chromatin patterns, and distinct micronucleoli. The undifferentiated tumor cells were usually isolated by sometimes occurred in aggregates. These tumor cells were spindle-shaped to fiber-shaped in appearance with indistinct cellular borders. The nuclei were round-to-oval and not uniform in shape. A fine granular chromatin pattern was dominant and nucleoli were often observed. These cells were determined to be of nonepithelial origin.
Conclusions: We present the cytopathological features a case of carcinosarcoma of the uterine corpus.View full abstractDownload PDF (7154K) -
Kiyokazu HARA, Takahiro YOSHIOKA, Yoshitaka KATSURA, Kazuhiro SATONAKA1999 Volume 38 Issue 5 Pages 431-435
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: It is difficult to diagnose pulmonary aspergillosis when no mycelium is found in histological specimens. We report here a case of pulmonary aspergilloma which was diagnosed by crystals in cytological specimens.
Case: A 64-year-old man with diabetes was admitted to our hospital on Oct. 14, 1997, because of cough, fever, and hemosputum. The chest roentogenographs showed a cavity with inflammatory changes at the upper and middle lesion of the right lung, a finding consistent with the presence of pulmonary tuberculosis. Bronchial brushing and washing smear revealed inflammatory exudate and numerous birefringent crystals which formed a sheath of needle-like crystals. Crystals which were colorless, and formed rosette, butterfly, pine needle, and flat structures were identified as calcium oxalate by histochemical analysis. As these findings sug-gested the presence of mycelia, a second bronchial fiberscopy was performed. Aspergillus niger was isolated by culture from the specimen obtained on the second bronchial fibers-copy. Interestingly, isolated Aspergillus niger was found to produce. calcium oxalate in the culture medium.
Conclusion: Even though mycelium is not detected in pulmonary cytological specimens, the finding of birefringent sheaf-like calcium oxalate crystals with inflammatory exudate suggests the presence of Aspergillus infection.View full abstractDownload PDF (4405K) -
Reiji HABA, Shoji KOBAYASHI, Hiroshi MIKI, Katuyuki NOMA, Yoshito YANO ...1999 Volume 38 Issue 5 Pages 436-440
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Mesenteric fibromatosis in Gardner's syndrome is very rare, and pre-operative diagnosis is difficult. We report a rare case of mesenteric fibromatosis which waseffectively diagnosed by a combination of frozen histological sections and cytological examination.
Case: A 29-year-old man was admitted with a complaint of diarrhea. He had a family history of polyposis. An endoscopic examination revealed polyposis in the colon. A large mass was observed around the ileum by CT. Colectomy, partial ileum amputation, and mass resection were performed. The solid tumor was about 14 cm in its longest diameter. By histological examination of a frozen section, mesenteric fibromatosis was suspected, but we could not exclude fibrosarcoma and leiomyosarcoma. Cytologically we could exclude other malignant tumors.
Conclusion: We could diagnose mesenteric fibromatosis in Gardner's syndrome and exclude other sarcomas by frozen histological examination with the aid of intra-operative cytology.View full abstractDownload PDF (9279K) -
Reiji HABA, Shoji KOBAYASHI, Hiroshi MIKI, Katuyuki NOMA, Yoshito YANO ...1999 Volume 38 Issue 5 Pages 441-444
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: A giant Brunner's gland hamartoma in the duodenum is very rare. It is usually small in size, and recognized as a submucosal tumor. Pre-operative diagnosis of Brunner's gland hamartoma is difficult, when it takes the shape of a giant pedunculated polyp. We report a rare case of giant Brunner's gland hamartoma which was effectively diagnosed by intra-operative cytological examination.
Case: A 70-year-old man with diabetes mellitus was incidentally found to have a giant polyp in the duodenum by an upper GI X-ray examination. An endoscopic examination revealed a giant polyp in the anterior wall of the duodenal bulb. The giant polyp was endoscopically resected for pathological diagnosis and therapy. The polyp was diagnosed as Brunner's gland hamartoma by intra-operative cytology.
Conclusion: Intra-operative cytological diagnosis diagnosis of a duodenal Brunner's gland hamartoma was possible, by excluding adenoma and adenocarcinoma.View full abstractDownload PDF (6336K) -
Yuta NAKAMURA, Tatsunari SATAKE, Saburo NAKAZAWA, Sonoko NATSUME, Masa ...1999 Volume 38 Issue 5 Pages 445-448
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: We report a rare case of pancreatic carcinoma which was diagnosed by aspiration cytology from a submucosal tumor.
Case: The patient was a 69-year-old male admitted to hospital for examination of a submucosal tumor of the stomach. The tumor was diagnosed with endoscopic ultrasonograpy as a cystic lesion, which was about 2 cm in diameter and located in the submucosal tissue of the stomach. Transgastric aspiration cytology was performed. The cytological specimens revealed several small clusters of columnar cells. The cells were comparatively small and round, and nuclei were located at periphery of the cytoplasm. Nuclear chromatins were deeply stained and nucleoli were enlarged. PAS staining was positive. Therefore these cells were diagnosed as adenocarcinoma. Operation was performed for a maligant tumor of the stomach, but a hard mass about 3 cm in diameter was found in the pancreatic body. The tumor was continuous with whe cyst wall in the stomach. Pathologic diagnosis was made as invasive ductal carcinoma of the pancreas. It was thought that pancreatic juice had run down the stomach wall to form a cyst. The ductal carcinoma tissue was exposed on the bottom of the cyst, and carcinoma cells covered a part of the interior surface.
Conclusion: Adenocarcinoma cells in the cytological specimens were considerde to be derived from a pancreatic carcinoma which had infiltrated the cyst.View full abstractDownload PDF (6296K) -
Rieko NISHIMURA, Masahiko TSUJIMOTO, Kazuo KUROKAWA, Ayumi TSUKIYAMA, ...1999 Volume 38 Issue 5 Pages 449-454
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Breast carcinoma with cartilaginous and/or osseous metaplasia is a rare tumor, and the findings of aspiration cytology remain poorly defined.
Case: A 47-year-old woman was referred to our hospital because of a rapidly growing left breast tumor. Aspiration cytology from the tumor revealed scattered multiple malignant chondromatous cells as well as a few sheets of benign ductal cells in the chondromatous matrix. These strongly suggested a malignant phyllodes tumor. A simple mastectomy with axillary lymph node dissection was performed. The excised tumor was 8×4×11 cm in size, white in color, and diffusely invaded to skin and adipose tissue. Histologically, the tumor was primarily composed of malignant chondromatous cells with various levels of differentiation, and with gradual differentiation into invasive ductal carcinoma in a few areas. The diagnosis of carcinoma with cartilaginous metaplasia was made. Misinterpretation of aspiration cytology findings was explained by the histological finding that chondrosarcomatous cells were mainly growing by lymph vessel invasion, preserving breast ductal architecture.
Conclusion: A breast carcinoma with cartilaginous metaplasia in which aspiration cytology suggested malignat phyllodes tumor was reported.View full abstractDownload PDF (8587K) -
Tadanori YAMAGUCHI, Yoshiaki IMAMURA, Tomoko SHIMAMOTO, Takako KAWADA, ...1999 Volume 38 Issue 5 Pages 455-461
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Papillary renal cell carcinoma (PRCC) is an uncommon subtype of renal cell carcinoma (RCC) that has distinctive pathologic and cytogenetic features, but for which only limited cytologic features have been reported.
Cases: Case 1 was a 60-year-old male, with two tumor lesions with fibrous capsules (both 1.5 cm in diameter) in the left kidney. Case 2 was a 62-year-old male who had a left renal tumor with a fibrous capsule, measuring 5.0×4.0cm in diameter. Cytologically, all tumors were characterized by papillary clusters of atypical cells with foamy cells. Histologically, they consisted of papillary growth of atypical cells with fibrovascular core containing various degrees of foamy cell infiltration. Immunohistochemical analyses showed that they were stained with markers for distal uriniferous tubuli such as EMA and cytokeratin 7. In situ hybridization (ISH) using a biotin-labeled DNA probe for the centromere area of chromosome 17 showed 17 trisomy in all tumors.
Conclusion: PRCC is thought to be a tumor with lower malignant potential compared with conventional RCC. Both our cases are alive without evidence of recurrence or metastasis about 1 year after the operation. Histologic subcategorization of a renal carcinoma as PRCC appears to have prognostic implications.View full abstractDownload PDF (12221K) -
A case of posterior mediastinal massforming extramedullary hematopoiesis associated with ThalassemiaMami HARUTA, Naruaki MATSUI, Takashi KITAMURA, Masafumi TAKIMOTO, Akir ...1999 Volume 38 Issue 5 Pages 462-466
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Extramedullary hematopoiesis (EMH) may arise in compensation for reduction in hematopoiesis associated with severe chronic anemia, leukemia or some other conditions. These lesions generally occur in the liver, spleen, adrenal, and rarely in soft tissue. In the present study, we report a case of EMH associated with thalassemia.
Case: A 47-year-old woman was noted to have a mass confined in the posterior mediastinum on chest X-ray at medical checkup 2 years before admission. The patient was admitted to our hospital because the mass was markedly enlarged. At MRI, a hemispherical mass, measuring about 4cm, was detected at the right edge of the thoracic vertebra. The mass was resected.
By Giemsa-stained imprint smear, cytological findings were similar to those of the normal bone marrow. On Papanicolaou stain, the following cells were identified: megakaryocytes which appeared as giant cells with abundant cytoplasm and a lobulated nuclei or with naked nuclei, orthochromic erythroblasts which appeared as small cells with cytoplasm stained by lightgreen or orange G and pyknotic nuclei, and granulocytes with horseshoe-shaped nuclei or segmented nuclei.
Conclusion: Although EMH located on the mediastinum is extremely rare, imprint cytology has diagnostic value if the patient suffers from severe hematopoietic disorders such as thalassemia.View full abstractDownload PDF (8353K) -
Hideko KIGUCHI, Yukio ISHIKAWA, Yoshikiyo AKASAKA, Shigeki ISHIGURO, H ...1999 Volume 38 Issue 5 Pages 467-471
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: The LE test examines the conversion of neutrophils to typical LE cells when normal neutrophils are incubated in vitro in the presence of serum factor obtained from individuals with systemic lupus erythematosus (SLE) However, this in vitro phenomenon may not represent the in vivo phenomenon occuring in celomic fluid.
Case: We report the cytologic detection of LE cells in pleural fluid collected from a 66-year-old male patient with severe SLE associated with neuropsychiatric systemic lupus erythematosus and psychoneurosis (CNS lupus). This cytologic detection of LE cells in vivo later contributed to definitive diagnosis of the disease in this patient. In pleural fluid, neutrophils and reactive mesothelial cells were abundant indicating inflammation, and many LE cells formed various patterns including rosettes. Besides the typical morphology of LE cells, disrupted cells that may have been caused by incorporation of LE factor were observed. The latter feature could be easily misdiagnosed to indicate degenerative cells.Conclusion: In the present study, cells in pleural fluid were stained with Papanicolaou and Giemsa stain and pathologic features of cells were compared with those observed in LE test. In vivo detection of LE cells, although these cells may frequently not be detected, should lead to definitive diagnosis of lupus erythematosus.View full abstractDownload PDF (4034K) -
Kunimitsu KAWAHARA, Makoto MOTOI, Hitomi KISHIMOTO, Satoko MAKITA1999 Volume 38 Issue 5 Pages 472-475
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Most undifferentiated carcinomas of the thyroid gland arise as a result of anaplastic transformation of a pre-existing tumor, usually papillary carcinoma.
Case: An 86-year-old woman with a past history of thyroid disease for 20 years was noted to have a rapidly growing nodule at the lateral neck. The cytologic smear of fine needle aspiration material revealed both elements of undifferentiated carcinoma and papillary carcinoma. We concluded that the cytological findings of undifferentiated carcinoma might be derived from a pre-existing lesion of papillary carcinoma. At autopsy, We were able to detect the sharply outlined nodule of papillary carcinoma within undifferentiated carcinoma.
Conclusion: The autopsy findings of nodular lesion composed of papillary carcinoma confirmed our cytological speculation.View full abstractDownload PDF (6083K) -
Significance of CK 20 on the diagnosis of Merkel cell carcinorna in cytologyChieko KIYA, Kunihiko WAKAKI, Chizuko MUROSAKA1999 Volume 38 Issue 5 Pages 476-481
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSBackground: Markel-cell carcinoma is rare and its differential diagnosis is difficult by routine stain in cytology. We report significance of CK 20 on the diagnosis of Merkelcell carcinoma in cytology.
Case: We report a 74-year-old female farmer with Merkel-cell carcinoma on the right upper eyelid. On stamp preparation of the operated specimen tumor cells cytologically showed monotonous, relatively small round nuclei. These cells had an increased N/C ratio with scanty clear cytoplasms and oval nuclei with fine granular, reticular chromatin, and a few small nucleoli, but no cytoplasmic button-like globules. On smear preparation of fine needle aspiration from the neck tumor, tumor cells formed in Indian file, partly scattered individually, and showed uneven, large to small nuclei with mitotic figures. Histologically, monotonous small round cells infiltrated solidly into the cutis and were negative for PAS reaction and reticulin silver impregnation. Immunohistohemically, tumor cells were diffusely and strongly positive for cytokeratin (CK 20, AE1), NSE, Chromogranin A, EMA, and partially positive for neurofilament, synaptophysin, but negative for S 100-P, vimentin, cytokeratin (AE3), CEA, LCA, and HMB 45. Electronmicroscopy indicated that these cells had a small number of neurosecretary granules, paranuclear intermediate filaments, and desmosome-like structures.
Conclusion: In differential diagnosis of three cases of Merkel-cell carcinoma and 8 types of other carcinomas, only Merkel-cell carcinoma was diffusely and strongly positive for CK 20. So, diffuse positivity of CK 20 is a significant finding in diagnosis of Merkel-cell carcinoma by cytology.View full abstractDownload PDF (11245K) -
Yuji ARAI, Hideo TESHIMA, Futoshi AKIYAMA, Masafumi TSUZUKU, Katsuhiko ...1999 Volume 38 Issue 5 Pages 482-483
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSThe present study was undertaken to explore the characteristics of yellow or yellowish brown mucin by Pap. staining in Adenoma malignum. A histochemical study on this tumor has revealed that the mucus of this tumor was different in nature from the normal cervical glands and well differentiated adenocarcinoma of the cervix.
Histochemical staining-(periodic acid schiff, PAS; alcian blue, AB; high iron diamine-alcian blue, HID-AB) was done using paraffin-embedded sections from 5 cases of adenoma malignum, 8 cases of well differentiated adenocar cinoma and 21 cases of normal cervix. A positive PAS reaction was observed in all normal controls, while all patient samples were positive but the intensity was weaker than the controls in adenoma malignum. A positive AB reaction was observed in all normal controls, but not in four cases of adenoma malignum and one case showed only a weak positive reaction. In HID-AB staining, both sulphomucin and sialomucin were predominant in well differentiated adenocarcinoma but both were negative in adenoma malignum, which is supposed to be neutral mucin on histochemical staining.View full abstractDownload PDF (2618K) -
Makoto OKADA, Kazuo YANAGINO, Masakuni TAKAHASHI, Yukihisa MINAGAWA, J ...1999 Volume 38 Issue 5 Pages 484-485
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSA case of malignant lymphoma, who could be diagnosed by cervical smear, is presented. A 43-year-old patient complained of genital bleeding and presented herself to Tottori University Hospital. Biochemical examination and blood analysis results were within normal limits. No lymph-node swellings were observed. Bone marrow biopsy revealed no abnormal cells. Cervical smear showed abnormal lymphocytes including immature lymphocytes. Immunochemical staining for cervical smear revealed positive LCA and L 26, and negative UCHL-1. Tissue diagnosis was malignant lymphoma, diffuse small cell type (B cell). She underwent radical hysterectomy followed by 6 courses of chemotherapy. She has been followed up without any evidence of recurrence.View full abstractDownload PDF (3834K) -
Yoshinobu SATOH, Hidekachi KUROTAKI, Masatoshi UESUGI, Soroku YAGIHASH ...1999 Volume 38 Issue 5 Pages 486-487
Published: September 22, 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSWe report a case of a 73-year-old woman with mucinous carcinoma of the breast mimicking cytological characteristics of secretory carcinoma. Fine needle aspiration (FNA) disclosed dispersed tumor cells with occasional aggregations consisting of “mucous globular structures (MGS) ” and “bunches of grapes”. There was only a small amount of mucinous materials in the background. Vacuoles or mucoid materials were often encountered in the cytoplasm showing signet-ring cell features. The findings in the present case suggest that FNA of mucinous carcinoma, especially mixed type, may present the formation of “MGS” and “bunches of grapes” previously described as a hallmark of secretory carcinoma.View full abstractDownload PDF (2948K) -
1999 Volume 38 Issue 5 Pages e1
Published: 1999
Released on J-STAGE: November 08, 2011
JOURNAL FREE ACCESSDownload PDF (108K)
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