The present study was undertaken to explore the characteristics of yellow or yellowish brown mucin by Pap. staining in Adenoma malignum. A histochemical study on this tumor has revealed that the mucus of this tumor was different in nature from the normal cervical glands and well differentiated adenocarcinoma of the cervix. Histochemical staining-(periodic acid schiff, PAS; alcian blue, AB; high iron diamine-alcian blue, HID-AB) was done using paraffin-embedded sections from 5 cases of adenoma malignum, 8 cases of well differentiated adenocar cinoma and 21 cases of normal cervix. A positive PAS reaction was observed in all normal controls, while all patient samples were positive but the intensity was weaker than the controls in adenoma malignum. A positive AB reaction was observed in all normal controls, but not in four cases of adenoma malignum and one case showed only a weak positive reaction. In HID-AB staining, both sulphomucin and sialomucin were predominant in well differentiated adenocarcinoma but both were negative in adenoma malignum, which is supposed to be neutral mucin on histochemical staining.
A case of malignant lymphoma, who could be diagnosed by cervical smear, is presented. A 43-year-old patient complained of genital bleeding and presented herself to Tottori University Hospital. Biochemical examination and blood analysis results were within normal limits. No lymph-node swellings were observed. Bone marrow biopsy revealed no abnormal cells. Cervical smear showed abnormal lymphocytes including immature lymphocytes. Immunochemical staining for cervical smear revealed positive LCA and L 26, and negative UCHL-1. Tissue diagnosis was malignant lymphoma, diffuse small cell type (B cell). She underwent radical hysterectomy followed by 6 courses of chemotherapy. She has been followed up without any evidence of recurrence.
We report a case of a 73-year-old woman with mucinous carcinoma of the breast mimicking cytological characteristics of secretory carcinoma. Fine needle aspiration (FNA) disclosed dispersed tumor cells with occasional aggregations consisting of “mucous globular structures (MGS) ” and “bunches of grapes”. There was only a small amount of mucinous materials in the background. Vacuoles or mucoid materials were often encountered in the cytoplasm showing signet-ring cell features. The findings in the present case suggest that FNA of mucinous carcinoma, especially mixed type, may present the formation of “MGS” and “bunches of grapes” previously described as a hallmark of secretory carcinoma.