目的および対象: 口腔白板症40例および口腔扁平苔癬12例の口腔剥離細胞診所見を, 臨床病型分類, 組織学的異形成および画像解析による結果と併せて比較検討したので報告する. 結果: 口腔白板症の細胞診の結果はClass I 15例, II 13例, III 11例, IV 1例であった.扁平苔癬は, Class I 6例, II 5例, III 1例であった.口腔白板症におけるClass分類と組織学的異形成との関係は, Class I (異形成なし80%, 軽度20%), Class II (異形成なし75%, 軽度17%, 中等度8%), Class III (異形成なし42%, 軽度16%, 中等度42%), Class IV (中等度100%) であり, 相関がみられた. 結論: 口腔白板症と扁平苔癬について光顕的観察による細胞学的鑑別点は見出されなかった.しかし画像解析の結果, 口腔白板症は光輝性, N/C比, ケラトピアリン穎粒数および核画積が有意に高かった.さらに口腔白板症について細胞学的異型性の有無で比較した結果, 異型性を有する口腔白板症は細胞質の光輝性, N/C比, ケラトピアリン顆粒数およびグロマチン量が増大している傾向がみられた.
To study the clinical significance of MMP-7 in colorectal cancers, we investigated 69 cases of imprint smear using immunocytochemistry and in situ hybridization. A total of 39 of 69 cases were positive for MMP-7 expression by immunocytochemistry. The expression of MMP-7 protein and mRNA were correlated. Positive cases of MMP-7 proteintented to be moderately differentiated tumors of the sigmoid colon or rectum, and invasive cases. The expression of MMP-7 and metastases to lymph nodes and liver were strongly correlated. In conclusion, MMP-7 may be a useful marker for invasion and metastasis in colorectal cancer.
Cytological findings of Leydig cell tumor of the testis are described. A 61-year-old male presented with left scrotal swelling and no hormonal manifestations. The castrated left testis contained a 4.6×4.1 cm, well-defined, yellowishbrown mass. Imprint cytological specimens showed polygonal, weakly cohesive tumor cells with round nuclei, fine granular chromatin, small nucleoli and light-green stained cytoplasm. Lipofuscin pigments were observed but no Reinke's crystals were found. The tumor cells were positive for cytokeratin and α-inhibin. Clinical information and consideration of related diagnoses are essential for the diagnosis of this tumor and α-inhibin immunostaining is helpful.
We report a rare case of glucagonoma of the pancreas, in which intra-operative cytology was helpful. By histological examination of a frozen section, it was difficult to determine whether the tumor was endocrine tumor, acinar cell carcinoma, or invasive ductal carcinoma. Cytologically it was diagnosed as endocrine tumor from the cytological features of noncohensive clusters, scanty, granular and rich cytoplasm, and round nuclei with even granular chromatin, there by excluding other malignant tumors.
We report the cytological findings of elastofibroma dorsi in the left subscapular region of a 55-year-old man and in the right subscapular region of a 58-year-old woman. The cut surface of the tumors was scraped with surgical blades. The scraped specimens showed fibroblast-like cells, light-green stained bead string-like or spherical structures and globules with serrated edge (petaloid globules) in Papanicolaou stain. The bead string-like structures were elastofibroma fibers. These elastofibroma fibers were also stained light-blue with Giemsa stain. To find elastofibroma fibers, we recommend the use of Giemsa stain rather thfan Papanicolaou stain.
We report a case of primary clear cell adenocarcinoma of the vagina in a 72-year-old woman. The tumor arised in the anterior to left vaginal wall at the introitus and showed signs of ulceration. A PAP smear revealed atypical round cells containing clear and abundant cytoplasm and round nuclei, with one to several prominent nucleoli in sheet-like clusters. Some nuclei with almost no cytoplasm and were scattered around the clusters. Histologically, the tumor consisted of two portions: a clear cell adenocarcinoma and a less-differentiated adenocarcinoma.