Background: Malignant pheochromocytoma cytology is reported in a very rare case showing morphologic alteration and increased proliferation following onset. Case: A 59-year-old man diagnosed with pheochromocytoma eventually died of repeated tumor recurrence over a period of 34 years. Histologically, tumor cells became small and uniform with decreasing granular cytoplasm and higher indices of cell proliferation and death one year prior to the patient's death. Stump specimens from a tumor at autopsy showed small tumor cells with prominent nucleoli and faintly granular cytoplasm. Some cells exhibited nuclear inclusion. Anisonucleosis was not conspicuous and occasional mitotic figures were seen. Conclusion: Cytological findings in our case are representative of malignant pheochromocytoma with high growth. Occasional mitoses and the small, uniform appearance of tumor cells may help in cytologically diagnosing malignancy in pheochromocytoma.
We report a patient with primary non-Hodgkin's lymphoma of the uterine cervix diagnosed by a uterine cervical smear that demonstrated abnormal lymphocytes. Tissue from the uterine cervix showed malignant diffuse largecell lymphoma. She experienced complete clinical remission following combined chemotherapy and irradiation therapy.
We report cytologic findings on a steroid lipid cell tumor of the ovary in a 45-year-old woman suffering from masculinization, including amenorrhea since the age of 41, hoarseness and hirsutism. Computed tomography revealed a right ovarian tumor. Serum androgenic hormones elevated before surgery returned to the normal range 10 days after the tumor was resected. An implint cytology specimen was composed of 2 types of tumor cell-those with abundant pale cytoplasm with occasional vacuoles and those with less cytoplasm stained well with light green reflecting the histological pattern of the tumor.
Imprinting cytology using fresh brain materials obtained surgically from a 65-year male with a sparsely granulated pituitary adenoma revealed relatively large cells with inclusion bodies in the nuclei and severe pleomorphism. Other cells were small and round with granular cytoplasm and small granular nuclei. Immunohistochemically, growth hormone stained in cytoplasm and CAM 5.2 at the nuclear periphfery with a granular pattern, but findings for ACTH, PRH, CK, and EMA were negative. These findings on the brain species revealed a sparsely granulated adenoma of the pituitary gland, confirming the utility of cytologoly, in addition to histopathology, in diagnosing brain tumors.
Cell transfer is applicable to broken slides and the preparation of multiple slides from a single smear on limited cytological material. We clarified the effectiveness of cell transfer by different mounting media and used resulting preparations to conduct immunocytochemical staining. Our results indicate that temperature and time in softening were affected by the properties (viscosity and xylene components) of mounting media. Cellular preservation and reactivity of most antigens are maintained following transfer. Cytological materials prepared by cell transfer are therefore suitable for immunocytochemical study.