Cytological findings of atypical adenomatous hyperplasia (AAH) in a 46-year-old man were compared to histological findings of biopsy specimens via fiberoptic bronchoscopy and videoassisted thoracoscopic surgery (VATS). The 12×10mm tumor, located at left S3, was diagnosed microscopically as AAH. Both the cytological specimen obtained by transbronchial needle aspiration and the histological specimens via transbronchial biopsy by bronchofiberscopy were evaluated. Cytological findings showed single atypical cells, discohesive clusters, and sheet-like arrangements. Round or oval nuclei were centrally located. and nuclear inclusion was often observed. Bi-and multinucleated atypical cells were seen, but not grooving or mitotic figurs, except rarely. These features may be important in distinguishing AAH from adenocarcinoma.
We report 2 cases of urachal carcinoma having totally different cytological features. Tumor cells in case 1 were highly atypical. having scanty mucin in the cytoplasm and growing in papillary patterns. Tumor cells in case 2 showed mild atypia with abundant mucin. Case 1 was difficult to distinguish cytologically from poorly differentiated transitional cell carcinoma. Case 2 was difficult to distinguish from adenoma. A definitive diagnosis of urachal carcinoma having these cytological features can only be made combining characteristic clinical findings.
We report a case of neuroendocrine carcinoma of the uterine cervix with HPV-18infcction in a 145-year-old wolnan. The tulllor arose in the uterine cervix. with the tumor surface necrotic and bioody. Cytology showed abundant tumor cell ina Ilecrotic background, with slnall rourld or short spindle nuclei, scant cytoplasnl or strippcd nuclei, scattered individually and aggregated in small clusters, and delicate or coars cgranular cllrolnatin. Hislologically, small turnor cell swere arranged in nests cell cords and a ribbonpattern.Thistumor wasdiagnosedasby spellout (ISH) a sneuroendocrinecarcillomawitll HPV-18illfectiollarisingintheuterinecervix. We fouu/d cytological examination to be useful in this diagrlosis.
Cell transfer is required to immunostain multiple antigens on cytology specimens. A variety of resins is used but the conventional sequence usually takes a couple of days to complete transfer. We cueated a quick method by covering the slide glass with a small volume of diluted Malinol in a process that is completed within 1 hour if the cover slip is peeled off the glass slide. Appropriate trimming of the thin solidified'ulalinol membrane into several pieces yields multiple specimens for immunocytochemical reaction, using a single cytological material.