The Journal of the Japanese Society of Clinical Cytology
Online ISSN : 1882-7233
Print ISSN : 0387-1193
ISSN-L : 0387-1193
Volume 44, Issue 4
Displaying 1-15 of 15 articles from this issue
  • Kenzo IKEMOTO, Kenji MASTUDA, Tomoko FURUYA, Atsunori OGA, Shigeto KAW ...
    2005 Volume 44 Issue 4 Pages 195-200
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Objective: We clarifed the usefulness of molecular cytogenetic analysis in diagnosing urothelial carcinoma of the urinary bladder.
    Study Design: Multicolor fluorescence in situ hybridization (FISH) using centrometic DNA probes for chromosomes 3, 7, and 17 and a locus specific probe for 9p21 (p16 INK4a) was conducted for 101 urothelial tumors, i. e., 44 bladder irrigation samples and 57 biopsy specimens.
    Results: Tumors were divided into diploid and aneuploid carcinoma based on nuclear DNA content. Numerical abnormalities were detected for at least one CEP probe in all urothelial tumors. Lowgrade carcinomas were characterized by a loss of 9p21 and diploid, whearas high-grade carcinomas showed large intercellular variations in CEP signal numbers and were aneuploid tumors. The numericalaberration in 9p21 signals was found in all tumors, indicating that the aberration in 9p21 signals is an indicator of urothelial carcinoma.
    Conclusion: The combination of FISH and LSC technologies is useful for diagnosing and evaluating malignancy in bladder urothelial carcinoma.
    Download PDF (1567K)
  • Miho KAWAI, Akinori ISHIHARA, Hideyuki KOYAMA, Shinji IHARA, Risa KIKU ...
    2005 Volume 44 Issue 4 Pages 201-204
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Objective: We analyzed fibrovascular clusters cytologically demonstrated in papillary breast tumor.
    Study and Design: We studied 24 cases of intraductal papilloma and 16 of papillary breast carcinoma.
    Results: Fibrovascular cluster materials were classified into two groups. Type I fibrovascular clusters showed vascular loops without epithelial cells. Type II consisted of fibrovascular stroma with epithelial cells. Type I was seen in 9 (56%) of 16 cases of papillary carcinoma, but none in the 24 cases of intraductal papilloma.
    Conclusion: Type I fibrovascular clusters help distinguish intraductal papilloma and papillary carcinoma of the breast.
    Download PDF (2300K)
  • Results for 16 years
    Mitsuko MATSUO, Junko SHIMOSAKO, Hideki NOGUCHI, Masayo KARIYAMA, Yoh ...
    2005 Volume 44 Issue 4 Pages 205-209
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Objective: Cytologic screening for endometrial cancer has been indicated only for women meeting certain conditions in uterine cervical screening. It is unknown, however, whether qualifications for corpus screening remain suitable under recent conditions in public health examination. To evaluate whether cytopathological screening for endometrial cancer should be done as part of the public health examination, we reviewed the results of cytopatholgical examination in Sakai City for the last 16 years.
    Study Design: We studied check-up forms, cytodiagnosis results, and follow-up survey results for 14, 188 women undergoing cytopathological screening for endometrial cancer in public health examinations between 1987 and 2002.
    Results: The percentage of women examined accounted for 5.7%, of whom 1.8% were cytologically identified as needing a biopsy. Afurther, 0.25% were diagnosed as having endometrial cancer. If the qualifications for cytopathologic uterine corpus screening were tentatively limited to those meeting all of the following conditions, however, i. e., over 50 years of age, postmenopausal, and having atypical genital bleeding in the last 6 months, both corpus screening and endometrial cancer detection would be more suited to the trial value calculated at the start of the cyotologic screening program for endometrial cancer as part of the public health examination.
    Conclusion: Our results suggest that new qualifications, including limited indications, should be considered for cost effectiveness in cytological examination programs for endometrial cancer screening.
    Download PDF (986K)
  • Shinichi IGARASHI, Toshiaki TAKAHASHI, Yuko OTSUKI, Mitsuro MAKITA, To ...
    2005 Volume 44 Issue 4 Pages 210-214
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Objective: We studied the clinical significance of cytology of chorionic villi in recurrent spontaneous abortion.
    Study Design: We analyzed histological and cytological findings in chorionic villi in recurrent spontaneous abortion, using chorionic villi specimens from episodic abortion in fertile cases as the control.
    Results: The incidence of apoptosis and infiltration of mononu-clear cells in recurrent spontaneous abortion increased both histology but also cytologically. No marked difference was observed in villous structure in either group.
    Conclusion: Histological and cytological findings in recurrent spontaneous abortion are similar to allograftic reaction. We surmise that many cases are related to immune reaction. Cytology studies of chorionic villi could thus contribute to the early diagnosis of etiology and early treatment of recurrent spontaneous abortion.
    Download PDF (1634K)
  • Hiroyuki OHSAKI, Muneo NAKAMURA, Kenichi NAGAMACHI, Tetsuro OGINO
    2005 Volume 44 Issue 4 Pages 215-218
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Objective: To determine optimal sample preparation for cellular yield in urine cytology without special apparatus.
    Study design: Eighty voided urine specimens from 38 patients with histologically confirmed G2/G3 urothelial carcinoma were prepared three ways:(1) dipping fixation in which smears made from the centrifuge sediment were put into 95% ethanol before drying;(2) spray fixation, in which a fixative was sprayed onto the wet smear; and (3) modified two-step fixation, in which centrifuge sediment was treated twice with alcoholic carbowax fixatives before smears were prepared. All smears were stained by the Papanicolaou method and studied for cellular yield. Cell yield was divided into 5 grades based on the number of diagnostic cancer cells:(-)(cell number, 0), (1+)(1 to 10), (2+)(11 to 50), (3+)(51 to 200), (4+)(201 or more).
    Results: Modified two-step fixation enhanced cellular yieldcomparedto other methods. Cell yields for (-), (1+), (2+), (3+), and (4+) in dipping fixation numbered 16 (20.0%), 19, 16, 10, and19 samples. Those in spray fixation numbered 6 (7.5%), 13, 20, 15, and 26 samples. Those in modified two-step fixation numbered 1 (1.3%), 15, 22, 11, and 31 samples.
    Conclusion: Modified two-step fixation yielded more diagnosticcancer cells than other methods.
    Download PDF (1350K)
  • Nana NUMATA, Tomoyuki SEKI, Sakiko HOSHIKAWA, Akio KAZAMA, Masayuki TA ...
    2005 Volume 44 Issue 4 Pages 219-223
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Background: Renal oncocytoma, a benign and relatively rare tumor, has an excellent prognosis if completely removed.
    Cases: We report three cases of renal oncocytoma. Histologically, tumor cells showed mild cytological atypia and eosinophilic granules of abundant cytoplasm on H-E sections. Oval nuclei are centrally located and showed few mitotic figures. Cytological examination of touch smear specimens showed intracytoplasmic vacuoles in 4.9% of tumor cells in renal oncocytoma. but fewer than 1.0% in renal cell carcinoma (RCC).
    Conclusion: Renal oncocytoma consists of tumor cells with slight atypia and eoshinophilic granular cytoplasm. Intracytoplasmic vacuoles are more frequently seen in renal oncocytoma than in RCC.
    Download PDF (3911K)
  • Mitsuhiko TAKAHASHI, Yoshitaka OHTA, Koichi HIGAKI, Sonoe ITOH, Takahi ...
    2005 Volume 44 Issue 4 Pages 224-229
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Background: We report a case of primary carcinoid of the liver diagnosed by fine-needle aspiration cytology.
    Case: A 37-year-old man suspected of having a liver abscess in ultrasonography was found in dynamic computed tomography (CT) to have masses were enhanced in the early phase. Peripheral regions of large tumors were enhanced at the S3 and S6 segments. Fine-needle aspiration cytology of the liver was performed under ultrasonographic guidance to S3. Atypical cells with “salt and pepper” nuclear chromatin and clear cytoplasm were found partly with a rosette formation of cells. Immunocytochemical studies showed positive staining with antibodies to chromogranin, synaptophysin, and neuron specific enolase, but negative to CEA, leading to a diagnosis of carcinoid. Subsequent chemotherapy was ineffective, with the patient dying 18 months later. An autopsy was done. Histological findings of the hepatic tumor showed solid, plain proliferation of round or oval tumor cells with a rosette formation. Immunohistochemical staining showed the same results as immunocytochemical staining. The definitive diagnosis was carcinoid originating in the liver, since no primary lesion was detected.
    Conclusion: Fine-needle aspiration cytology is very useful for accurately diagnosing primary carcinoid of the liver.
    Download PDF (3884K)
  • Nozomu KUROSE, Eriko KINOSHITA, Michiho TAKENAKA, Toshie TERAUCHI, Man ...
    2005 Volume 44 Issue 4 Pages 230-234
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Background: We report a case of histological, imprint-cytologicy and electron microscopicy findings on localized epithelial mesothelioma with extramural growth of the jejunum.
    Case: A 32-year-old woman admitted to give birth to her second child 1 year ago was laboratory examination after a normal delivery to have progressive anemia and elevated CRP. Computed tomography showed a solid intrapelvic mass with a 5.5 cm cystic lesion in the jejunum, necessitating partial jejunal resection. The jejunal tumor showed extramural growth. Histologically, the tumor was epithelial mesothelioma with cystic formation and severe inflammatory cell infiltration. Electron microscopy examination showed long microvilli. No recurrence or metastasis has been seen in the 1.5 years since surgery.
    Conclusion: Cytologically, it is difficult to differentiate between reactive mesothelial cells and tumor cells, although a tumor and malignant mesothelioma an be differentiated. In tumor etiology, we concluded that no relation ship eristed between asbestos and chronic peritoneal irritation because no mesothelial hyperplasia or inflammatory reaction in the peritoneum was seen. Stromal inflammatory cells infiltration suggests a tumor associated immunological tissue reaction. Compared to diffuse mesothelioma, prognosis in this case may be extremely good, but that careful follow-up is needed.
    Download PDF (4519K)
  • Naotoshi SAITO, Syuuji NAKAMURA, Shigeru MASUO
    2005 Volume 44 Issue 4 Pages 235-239
    Published: July 22, 2005
    Released on J-STAGE: December 05, 2011
    JOURNAL FREE ACCESS
    Background: We report a rare case of urothelial carcinoma of the renal pelvis with trophoblastic differentiation.
    Case: A 59-year-old man with hematuria was found in retrograde pyelography, to have a tumor of the right renal pelvis. Cytological specimens of right pelvic washing consisted of papillary clusters and scattered atypical cells identical to urothelial carcinoma, and multinucleated giant cells with cytoplasmic vacuoles and mononuclear cells with large nuclei containing coarse chromatin. Histological examination of the tumor showed papillary urothelial carcinoma (grade 2 dominant) associated with syncytiotrophoblast-like giant cells. Multiple foci of giant cells and mononuclear cells were positive for hCG in immunohistochemistry.
    Conclusion: Multinucleated giant cells in renal pelvic washing showed trophoblastic differentiation in urothelial carcinoma.
    Download PDF (2234K)
  • Ken-ichirou YONEDA, Shinobu UMEMURA, Hitoshi ITOH, Akihiko SERIZAWA, N ...
    2005 Volume 44 Issue 4 Pages 240-244
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Background: We report a case of human herpes virus 8-associated primary effusion lymphoma in an elderly HIV-seronegative male, diagnosed through pericardial fluid and pleural effusion analysis.
    Case: An HIV-seronegative 82-year-old man was diagnosed as having esophageal cancer 3 years ago, and was treated with local radiation therapy. Pericardial effusion and pleural effusion were detected by chest X-ray images 2 years ago. However, the origin of the pericardial effusion and pleural effusion could not be detected. He was admitted to our hospital with dyspnea and heart failure. Echocardiography Echocardiography and chest X-ray photography showed massive pericardial fluid and pleural effusion. Pericardial and pleural drainages were performed. Cytological findings of the pericardial fluid and pleural effusion showed a large number of atypical lymphocytes, which were large and possessed irregular-formed nuclei with abundant cytoplasm. Multinucleated giant atypical lymphocytes were also found. These atypical lymphocytes were positive for CD20, HHV-8 and negative for CD45RO in immunocytochemical stainings of cytology preparations. The patient was diagnosed as having primary effusion lymphoma from these features.
    Conclusion: Primary effusion is predominantly found in HIV-seropositive patients and rarely in HIV-seronegative patients. Immunocytochemical stainings of cytology preparations and cell blocks are useful in the diagnosis of primary effusion lymphoma.
    Download PDF (1818K)
  • Shunichi SASOU, Naoki YANAGAWA, Miyuki UEMATSU, Yuki TAKAHASHI, Senji ...
    2005 Volume 44 Issue 4 Pages 245-249
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Background: No previous reports have, to our knowledge, described the urine cytology of urinary bladder sarcomatoid carcinoma.
    Cases: Case 1: A 4cm nodular lesion found in the urinary bladder of a 59-year-old woman showed larger high-grade atypical cells characterized by a thick nuclear rim, constricted nuclei, or multinuclei in urine cytology. Histologically, scattered foci consisting of highgrade malignant epithelial cells were found in sarcomatoid lesions.
    Case 2: A nodular tumor accompanied by small G2 papillary urothelial carcinomas was observed in the urinary bladder of an 87-year-old woman the fourth recurrence. Urine cytology showed papillary-like cell clusters consisting of middle-sized atypical urothelial cells and other more atypical cells, i. e., irregular cells sized from small to large, round cells with round and irregular-shaped nuclei having increased chromatin, and spindle cells with nuclei situated at cell center. Histologically, the tumor consisted of atypical spindle cells that produced many foci of cartilage and bone tissue.
    Conclusion: When strangely shaped atypical cells in addition to ordinary urothelial cancer cells are found during the daily urine cytology, sarcomatoid carcinoma should be posed as a possible diagnosis.
    Download PDF (2380K)
  • Chie HAYAKAWA, Akihiro HOSHIKAWA, Tomoyuki SEKI, Sakiko HOSHIKAWA, Aki ...
    2005 Volume 44 Issue 4 Pages 250-254
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Background: Solitary fibrous tumor (SFT) is a relatively rare soft tissue neoplasm consisting of spindle-shaped fibroblast-like cells with collagen fibers. Signee cytological SFT features have rarely been documented. we report cytological features of a malignant solitary fibrous tumor originating in the pleura.
    Case: A pleural mass pointed out in a 71-year-old man in 1997 was biopsied and diagnosed as SFT, and initial resected in 1998. Recurrence in 2003 and a second recurrence in 2004 were detected and resected. Cytological specimens were taken at the first operation and the second recurrence. Sheet-like clusters consisting of spindleshaped cells were seen in the first operation specimen. In the specimen from the second recurrence. small clusters of neoplastic cells with epithelioid features were present. Many neoplastic cells be came round or oval. Histologically. the tumor consisted of spindleshaped cells with stromal collagen in the first operation specimen. Higher cellularity of neoplastic cells with minute foci of necrosis, thought to be a conspicuous change toward malignancy, was seen in the second recurrence specimen.
    Conclusion: Round morphological change in neoplastic cells and epithelioid cell clusters may be an important features in malignant transformation in SFT.
    Download PDF (5251K)
  • Takashi UMEZAWA, Takashi NIKAIDO, Setsuko HARUMA, Ken NAKAJIMA, Ayumi ...
    2005 Volume 44 Issue 4 Pages 255-258
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    Background: Fine needle aspiration cytology plays an important role in making a definitive diagnosis of soft-tissue tumors. We report a case of extraskeletal myxoid chondrosarcoma encountered by us recently, with emphasis on the cytological findings.
    Case: A 47-year-old man. Imaging revealed a multilobular cystic mass, 10×15cm in size, arising in the skeletal muscles in the posterior aspect of the left thigh. The lesion appeared as a low-signal-intensity area on T1-weighted MR images and high-signal-intensity area on T2-weighted MR images. A massive sarcoma with myxoid stroma was suspected. Fine needle aspiration cytology revealed round or oval cells in a background of myxoid stroma containing microfibril-like structures. Diverse patterns of cellular arrangement were observed, such as small clusters of pair cells in a funicular configuration. The nuclear-cytoplasmic ratio was high. Fine granular chromatin was observed in the nuclei, and wrinkles and constrictions were observed at the nuclear edge. Histologically, the extracted tumor exhibited lobulation with an abundance of a myxoid stroma. The tumor cells exhibited syncytial growth, and were arranged in cords or in small clusters. Immunohistochemically, the tumor cells were positive for S-100 protein and vimentin.
    Conclusion: Thus, the characteristic cytological findings were that of the tumor cells arranged in diverse patterns, in an abundant myxoid background containing microfibril-like structures, and wrinkles and constrictions were found at the edge of the cellular nuclei. However, it was not so easy to make a differential diagnosis from other myxoid tumors only from the cytological features.
    Download PDF (2999K)
  • Shigeo SEINO, Takashi KITAMURA, Atsuko MASUNAGA, Genshu TATE, Toshiyuk ...
    2005 Volume 44 Issue 4 Pages 259-260
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    We report the typical cytological features in a case of lymphoepithelioma-like carcinoma (LELC), rare among uterinecervical malignant neoplasms. A 61-year-old woman seen for irregular genital bleeding was found in 2 cytological examinations tohave neoplastic cells with high N/C ratio and conspicuous nucleoli as a cluster or a single cell, with numerous normal squamous epithelia in the background. Cytology also showed lymphocytic infiltration into the neoplastic cell cluster. The cytological diagnosis was carcinoma in situ because this lesion lacked a neoplastic background, but the definitive pathologic diagnosis from the conization specimen was relatively early-stage LELC. This case suggests the need to consider the possibility of LELC when observing lymphocytic infiltration into a cluster of undifferentiated neoplastic cells having prominent nucleoli.
    Download PDF (1209K)
  • Hiroaki KASE, Noriko GOTO
    2005 Volume 44 Issue 4 Pages 261-262
    Published: July 22, 2005
    Released on J-STAGE: November 08, 2011
    JOURNAL FREE ACCESS
    We could rule out malignancy and suggest endometriosis based on imprint cytology findings for an umbilical specimen. A 44-year-old woman suffering abdominal discomfort for two weeks and cyclic umbilical pain for eight years had no history of previous abdominal surgery. Examination showed a firm round 3×2cm nodule at the umbilicus and 10cm uterine myoma. The umbilical mass was excised during hysterectomy. Imprint cytology yieled tubular clusters appearing to be normal proliferative-phase endornetriosis, and endometrial stromal cells.
    Download PDF (1262K)
feedback
Top