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Volume 49 (2010)
- Issue 6 Pages 387-
- Issue 5 Pages 321-
- Issue 4 Pages 237-
- Issue 3 Pages 165-
- Issue 2 Pages 99-
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Volume 49, Issue 6
Displaying 1-13 of 13 articles from this issue
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Original Articles
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Eisaku TOJI, Masatsugu UEDA, Noriko KUNIFUJI, Michiko YAMAMOTO, Hiromi ...2010 Volume 49 Issue 6 Pages 387-392
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSObjective : We evaluated cervical cancer screening status based on The Bethesda System (TBS) in Osaka, focusing on atypical squamous cells of undetermined significance (ASC-US) and human papilloma virus (HPV).
Study Design : We studied TBS-based cervical cancer screening status in 18 Osaka Prefecture cities and towns. Subjects numbering 1,176 subjects were seen at Osaka Cancer Prevention and Detection Center colposcopy clinics for cytology, colposcopy, histology, and high-risk HPV-DNA testing using hybrid capture (HC)-2.
Results : TBS and HPV-DNA knowledge is now relatively widespread, but not TBS knowledge on the need for repeated cell sampling for inadequate specimens, clinical ASC-US management, or cytological high-grade cervical squamous intraepithelial lesion (HSIL) classification. The positive high-risk HPV-DNA rate among our 1,176 cases was 38%. Cervical intraepithelial neoplasia (CIN) 1 or 2 was detected in 11 of 15 (83%) positive for high-risk HPV-DNA with ASC-US. The persistence or disappearance of CIN lesions in ASC-US was closely associated with high-risk HPV-DNA status.
Conclusion : The clinical importance of TBS and ASC-US must be disseminated more widely among subjects in Osaka Prefecture. High-risk HPV-DNA testing appears to be quite useful in managing cases involving ASC-US.View full abstractDownload PDF (314K) -
Kaori OKAYAMA, Mitsuaki OHKODO, Tomoko KUMAGAI, Hiromi YABUSAKI, Haruk ...2010 Volume 49 Issue 6 Pages 393-399
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSObjective : We visualized single-copy HPV-DNA using in situ PCR, comparing staining results of in situ PCR to CSA-ISH.
Study Design : Study materials were cell lines HeLa, SiHa, and HL60, and liquid cervical cytological specimens from 5 women diagnosed with LSIL. We initially studied optimum conditions for pretreatment and in situ PCR, comparing staining results between in situ PCR and CSA-ISH for different fixed times.
Results : Pretreatment with 0.01% trypsin at between 2 and 2 min 50s gave optimum results. Optimum conditions for in situ PCR with HPV-18 primers were initial DNA denaturation for 1 min followed by 25 amplification cycles consisting of denaturation for 30s at 90°C, primer annealing for 30s at 59°C, and extension for 30s at 72°C. HeLa cells showed diffuse nuclear positivity for HPV-18 under these conditions. Compared to both types of staining, which differ with fixed times, in situ PCR staining was unaffected by fixed time. SiHa cells showed specific nuclear positivity for HPV-16 in in situ PCR and CSA-ISH.
Conclusion : Using this improved in situ PCR, we visualized single-copy HPV-DNA in cells from smear preparations fixed in ethanol.View full abstractDownload PDF (537K) -
—Cytological findings and histological background—Naruaki MATSUI, Kanako WAKUI, Hitoshi ITOH, Takashi KITAMURA, Hiroshi ...2010 Volume 49 Issue 6 Pages 400-405
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSObjective : We analyzed uterine-body endometrioid adenocarcinoma (EMA) cells appearing in the peritoneal cavity.
Study Design : Cases were 33 EMA specimens surgically resected from 2000 to 2006 and consisting of 17 G1 (51.5%), 13 G2 (39.4%), and 3 G3 (9.1%). Focusing on squamous metaplastic-like cells (SMC) using immunohisto/cytochemical tests, we analyzed cytohistological findings and their association with pathological conditions such as histological grade, depth, tumor size, and location.
Results : Peritoneal cytological findings were as follows : large to small papillary G1 or G2 clusters and scattered G3 patterns. SMC appeared in clumps and scattered : 1+at 33.3%, 2+at 55.6%, 3+at 11.1%, and 61.1% for G1, 33.4% for G2, and 5.5% for G3. These features were found to be related to diffuse tumor location but not to invasion depth or tumor size.
Conclusions : SMC appears to be a hallmark in peritoneal uterine-body EMA cytology, especially in G1 or G2 diagnosis.View full abstractDownload PDF (749K)
Clinical Articles
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Namiki KAWANISHI, Hiroshi SONOBE, Yoshiaki NORIMATSU, Toshiaki KAMEI, ...2010 Volume 49 Issue 6 Pages 406-411
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSBackground : Solid neuroendocrine carcinoma, a rare neuroendocrine tumor, arises primarily in the breast. We report a case of solid mammary neuroendocrine carcinoma and its cytological and immunocytochemical features.
Case : A 67-year-old woman having a tumor detected in the right breast (CD area) by ultrasound sonography was diagnosed histologically and immunohistochemically from needle biopsy tumor tissue as having solid neuroendocrine carcinoma. Electron microscopy showed neuroendocrine granules in tumor cell cytoplasm. Aspiration biopsy cytology showed numerous tumor cells and characteristic plasma cell-like features with slightly atypical small round eccentric nuclei. Most tumor cells obtained by cell transfer were strongly positive for chromogranin A, synaptophysin, CD56, and neuron-specific enolase, confirming neuroendocrine differentiation.
Conclusion : Immunocytochemical studies using cell transfer for tumor cells were very useful in definitively cytologically diagnosing this solid neuroendocrine carcinoma case.View full abstractDownload PDF (766K) -
Akemi NOZAWA, Hiroto UEDA, Asako KANAI, Yuichi SIMOE, Yoshihide IKAWA, ...2010 Volume 49 Issue 6 Pages 412-418
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSBackground : Extraovarian peritoneal serous papillary carcinoma (EOPPC), a rare condition featuring peritoneal carcinomatosis, uninvolved or minimally involved ovaries, and no identifiable primary tumors, has a histological pattern similar to ovarian serous papillary carcinoma.
Case : A 56-year-old woman with EOPPC and abnormal endometrial cytology changes, reported no specific symptoms. Endometrial cytology showed classV with enlarged atypical cell clusters and endometrial biopsy indicated endometrioid adenocarcinoma with suspected endometrial cancer. Total abdominal hysterectomy and bilateral salpingooophorectomy and lymph node resection showed no ascites or peritoneal dissemination. Washing ascites cytology showed many large malignant cells in small papillary clusters. Histopathological findings showed no malignant cells in the endometrium, but the left mesovarium and perimetrium showed small fragments of papillary adenocarcinoma. Both ovaries were normal in size, but the ovarian serosal surface was microscopically involved. Immunohistochemically, tumor cells were positive for p53 and MIB-1 but negative for calretinin, yielding a diagnosis of EOPPC.
Conclusion : Endometrial cytology is useful in diagnosing EOPPC.View full abstractDownload PDF (1029K) -
Naoko YOSHIMOTO, Ritsuko IMAI, Nobuyuki TANAKA, Tatsunari SATAKE, Yosh ...2010 Volume 49 Issue 6 Pages 419-424
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSBackground : We report cytological and histological findings on breast cancer with cartilaginous and osseous metaplasia, focusing on cells from “transitional” tumor tissue.
Case : A 74-year-old-woman seen for a left-breast mass was confirm in mammography, ultrasonography and computed tomography (CT) to have a round tumor 4 cm in diameter below the left areola. Fine-needle aspiration cytology yielded a diagnosis of carcinoma necessitating surgical resection.
Cytological specimens showed many isolated variable-shaped malignant cells and a few cell clusters showing mild accumulation with loose cell connections positive for vimentin but negative for keratin in immunostaining. Histologically, adenocarcinomatous, cartilaginous, and osseous components occurred together with “transitional” tissue between components. Transitional tissue showed the same immunostaining reaction as cell clusters in cytological smears.
Conclusions : Cell clusters in cytological aspiration biopsy smears of the breast may be apt to be diagnosed as carcinoma. In this case, however, they were derived from transitional tissue. Cells in clusters not showing both severe accumulation and tight connections accompanied by many isolated malignant cells in breast smears must be considered as possible breast cancer with cartilaginous and osseous metaplasia.View full abstractDownload PDF (876K)
Special Articles <Quality assurance to improve sampling and smearing technique on cytology>
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[in Japanese], [in Japanese]2010 Volume 49 Issue 6 Pages 425
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSDownload PDF (86K) -
Atsushi KISANUKI, Junko SEKIYA, Ayumi SANO2010 Volume 49 Issue 6 Pages 426-430
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSObjective : Cytological examination of bronchoscopy is important and have diagnostic figure. We improve the quality of cytological sample obtained by bronchoscopy.
Study Design : From January to September 2008, we improved treatment of cytological examination ; meeting with bronchologist, sample treatment and fixation by cytotechnologist, and induced of liquid-based cytology (LBC).
Results : Communication and sample treatment by cytotechnologist improved the quality of sample such as proper number of glasses and shortness time before fixation. Appropriate sample treatment by the cytotechnologist is more important than the type of cytology—brushing or bronchial—used.
Conclusion : Accurate diagnosis requires clear communication between the bronchologist and cytotechnologist and appropriate sample treatment by the cytotechnologist.View full abstractDownload PDF (507K) -
Yukari YANASE, Mitsuyoshi HIROKAWA, Miyoko MAEKAWA, Seiji KUMA, Nobuyu ...2010 Volume 49 Issue 6 Pages 431-436
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSAccording to Japan’s general rules for describing thyroid cancer aspiration and smear techniques should be reexamined when the incidence of inadequate materials exceeds 10% of all cytology cases. In discussing quality improvements, we recommend that aspiration be ultrasound-guided and target the most suitable portion for diagnosis. Appropriate smear technique requires the careful observation of aspirated materials. We found that the incidence of inadequate materials was greatly reduced by informing the quality of the preparations to aspirators. To improve preparation quality, aspirators, cytotechnologists, and cytopathologists must actively cooperate in mutually improving quality.View full abstractDownload PDF (642K) -
Sadayuki KABA, Yoshiro TOKORO, Midori SUZUKI, Masako KOBAYASHI, Junko ...2010 Volume 49 Issue 6 Pages 437-442
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSObjectives : We studied quality control for improving cellular specimen adequacy in lymph-node fine-needle biopsy (FNB).
Study Design : Subjects numbered 1,850 cases, i. e., 1,691 from 2002 to 2007 at site 1 and 159 from 2005 to 2007 years at site 2, with FNB as shown in Figure 1 as follows :
1. A cart with a FNB needle, glass specimen slide, fixative, Diff-Quik staining material, and a microscope were taken to the examination site by a physician and a technologist.
2. At site 1, Echo-guided FNB lymph node aspiration conducted by inserting a 24 G needle, turning it without aspiration, withdrowing it, attaching it to a syringe, then extruding the cellular specimen onto the specimen slide.
3. The smear was made by pressing a second specimen slide onto the specimen on the first slide.
4. Sampling was confirmed using the microscope immediately after Diff-Quik staining.
5. Diff-Quik and with Papanicolaou staining have been used to improve cytodiagnostic precision.
At site 2, echo-guided FNB with aspiration cytology was conducted using a “pistol” attached to a syringe (20 ml) with a 22 G needle. The cellular sample smear was made and immediately fixed in 95% ethanol, then stained by the Papanicolaou method in the laboratory.
Results : The annual average specimen inadequacy at site 1 from 2002 to 2004 was 9.6%, and 3.8% from 2005 to 2007, versus an annual average of 3.1% at site 2.
Conclusions : Annual average specimen inadequacy in echo-guided lymph-node FNB was less than 5%. A needle must hit a lesion under ultrasonography to improve specimen adequacy, and physicians must be trained sufficiently in echo-guided FNB cytology. The physician-cytotechnologist team is thus desirable in planning effectiveness for the puncture site and stable specimen adequacy.View full abstractDownload PDF (548K) -
Atsushi FURUHATA, Yasuaki HIROOKA2010 Volume 49 Issue 6 Pages 443-448
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSTo standardize cytological bile juice preparation, we examined the following points and their usefulness using brush epithelial gallbladder cells from 15 cholecystolithiasis cases : (1) cytomorphological changes after cells were suspended in additive-free bile juice and the effects of different preservative fluids on (2) nuclear preservation and on (3) cell collection.
Brush cell nuclei in additive-free bile juice showed reduction and pyknosis within 5 minutes after cell suspension, with maximum change noted within 4 hours. Cell suspended in bile juice including CytoRich® or YM® fluid nuclei were well preserved up to 24 hours. A significant number of cells were collected using bovine serum albumin, Roswell Park Memorial Institute (RPMI) 1640 medium, 50% ethanol, CytoRich, YM, and Postsampler® fluids. Gallbladder cancer cell nuclei were well preserved after adding YM fluid to routine bile juice for 48 hours. In contrast, nuclear changes indicated poor preservation of brush cells or cancer cells collected in bile juice alone.
Preservation and cell collection results were good for alcohol-based fluids, indicating their effectiveness for use combined with routine bile juice and enabling accurate diagnosis.View full abstractDownload PDF (774K)
Brief Notes
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Hirofumi SAKAMOTO, Kazuki USHIMARU, Noriyuki MIWA, Takuji TANAKA2010 Volume 49 Issue 6 Pages 449-450
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSOur findings demonstrate that uterine cervix LBC specimens are applicable to cell blocks useful for histopathological and immunohistochemical study in accurate diagnosis in potential or actual malignancy.View full abstractDownload PDF (338K) -
Mamoru MIKI, Katsumi HAGIWARA, Hiroshi HIRANO, Satoko BABA, Keiji NAKA ...2010 Volume 49 Issue 6 Pages 451-452
Published: 2010
Released on J-STAGE: January 28, 2011
JOURNAL FREE ACCESSA 66-year-old woman with neuroendocrine carcinoma in situ (NE-DCIS) cytologically examined for a nipple discharge was found to have small to moderate irregular cells with a low N/C ratio and mild hyperchromatism against a hemorrhagic background. Immunocytochemistry showed tumor cells to be positive for synaptophysin. Histologically, round, uniform tumor cells with eosinophilic cytoplasm were arranged in a solid papillary pattern within the duct. Tumor cells contained vascular networks and were immunohistochemically positive for synaptophysin and chromogranin A, yielding a definitive diagnosis of NE-DCIS. The small to moderate irregular cells with their low N/C ratio and mild hyperchromatism with hemorrhage and positive immunocytological staining enabled preoperative diagnosis.View full abstractDownload PDF (259K)
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