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Volume 51 (2012)
- Issue 6 Pages 395-
- Issue 5 Pages 315-
- Issue 4 Pages 235-
- Issue 3 Pages 159-
- Issue 2 Pages 93-
- Issue 1 Pages 1-
Volume 51, Issue 4
Displaying 1-12 of 12 articles from this issue
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Original Articles
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Rieko NISHIMURA, Norihiro TERAMOTO, Tamami YAMAMOTO, Akihiro KAGAWA, S ...2012 Volume 51 Issue 4 Pages 235-240
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSObjective : Cell-blocks are superior to smears in morphological interpretation and for adding special staining. The aspiration cytology cases we present show how cell-blocks aid in diagnosis as discussed in this article.
Study Design : Aspirated cell-block specimens were prepared for with an Ebis 1 cell-block preparation kit (Asia Kizai, Co., Ltd.). One hematoxylin-eosin and several special stainings were done.
Results : Aspirated-specimen cell-blocks were made from 25 neck lesions-17 from lymph nodes, 3 from the thyroid gland, and 5 from neck tumors−plus 2 from soft tissue lesions. Cancer metastasis was detected in 12 of 17 lymph nodes. While cell-block diagnoses in thyroid and neck tumors were 2 cancer and malignant lymphomas each. Follow-up aspiration cytology for the 2 soft-tissue lesions detected metastasis in a subject who had had cancer. Ten of the 27 lesions detected no diagnosis.
Conclusions : Cell-block preparation is useful in diagnosing lesions whose biopsy is difficult to due to site, and for severely compromised subjects. Cell-block preparation is also useful in differentiating between carcinoma and malignant lymphoma diagnosis.View full abstractDownload PDF (1012K) -
Sachiko SEYAMA, Hidehiko MASAOKA, Mieko DOI, Tadaaki MOCHINO, Mineko K ...2012 Volume 51 Issue 4 Pages 241-247
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSObjective : To improve diagnostic accuracy in cases of Warthin’s tumor in fine-needle cytology specimens of the salivary gland.
Study Design : Cases were 45 salivary-gland aspiration biopsy specimens histologically diagnosed as Warthin’s tumor.
Results : Of the 45 cases, 31 cases showed oncocytic cells. Of the 31, 19 showed two-cell patterns compose of oncocytic cells and lymphocytes, and 12 others background debris, granular materials, and degenerated oncocytic cells, but no conspicuous lymphocytes. Eight cases showed metaplastic cells of various morphologies, including normal squamous cells and atypical cells.
Conclusion : Identifying oncocytic cells is important in correctly diagnosing Warthin’s tumor. The presence of background debris, granular materials, and degenerated columnar oncocytic cells is a diagnostic clue if the two-cell pattern is not clear. The absence of oncocytic cells may at most lead only to the diagnosis of cystic lesions.View full abstractDownload PDF (877K) -
Akemi TAGUCHI, Mitsutoshi SHIBA, Hitomi TAJIMA, Atsuko SODA, Takeichir ...2012 Volume 51 Issue 4 Pages 248-255
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSObjective : Sputum cytology has been proved to be effective to detect the central type squamous cell carcinomas (SCC), but has not been proved to be effective to detect the peripheral type SCC. We analyzed the efficacy of sputum cytology for the detection of the peripheral type SCC with mass screening.
Study Design : Collected sputum smears of 22 patients detected and diagnosed as having the peripheral type SCC, and the same number of samples from the central type SCC patients were analyzed cytologically.
Results : During this period, 156 lung cancers were found, and among them, 16% were peripheral SCCs. The numbers of atypical squamous cells appearing in the sputum of early stage peripheral SCCs were small and incoherent compared to advanced SCCs, and degeneration of atypical squamous cells showed the same tendency. Compared with central SCCs, bright yellow and round keratinizing atypical squamous cells were significantly dominant in peripheral SCCs.
Conclusion : Analysis of atypical squamous cells in the sputum of the SCC patients was effective to estimate the sites and extent of lesions. In order to detect early lung SCC, an adequate amount of sputum specimens and their intensive screening are deemed necessary.View full abstractDownload PDF (839K) -
Toshiharu MAEDA, Katsumi KITO, Keizo FURUYA, Makiko KATO, Rie MORI, Yu ...2012 Volume 51 Issue 4 Pages 256-260
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSObjective : We examined Papanicolaou-stained liquid-based cytology (LBC) and LBC-p63 immunostaining in fine needle aspiration cytology (FNAC) of the breast.
Study Design : We made two LBC slides from materials left in the syringe after conventional slides were made in FNAC of the breast. When enough epithelium cells were seen on the Papanicolaou-stained LBC slide, we used p63 immunostaining on the remainder.
Results : In 248 breast FNAC cases seen from September 2010 to May 2011, we examined 100 cases having enough epithelium cells from an LBC slide. Of the 100, 63 were confirmed histologically. 57 were malignant, and 6 benign. The p63-positive cell counts were significantly lower in cases of malignancy than in benign cases.
Conclusion : LBC-p63 immunostaining is useful for differentiating benign and malignant cases in FNAC of the breast.View full abstractDownload PDF (548K) -
Kanako WAKUI, Naruaki MATSUI, Hiroshi KAJIWARA, Hitoshi ITOH, Kimitaka ...2012 Volume 51 Issue 4 Pages 261-266
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSObjective : The aim of this study was to improve diagnostic accuracy simply and efficiently by preparing paraffin-embedded cell blocks using Ebis 1 ver. 2/Hold Gel 110.
Study Design : Subjects numbered 193 seen at a gynecology clinic. Using Hold Gel in routine endometrial cytology, we examined its usefulness.
Results : Based on endometrial cytological evaluation, the 193 were divided into 174 negative cases, 8 indeterminate, and 3 with positive lesions. Evaluation using the cell block method resulted in 143 normal or benign, 6 suspected of endometrial hyperplasia, and 3 with adenocarcinoma. Cytological diagnosis and cell block concordance were 100% for negative, 37.5% for indeterminate, and 66.7% for positive. Cytologically indeterminate cases were divided into negative and positive using a cell block method. Cytological diagnosis and cell block method concordance were similar to those of histological examination.
Conclusion : It appears to help in diagnosing endometrial lesions to observe both endometrial cytology specimens and slides from cell blocks using Hold Gel.View full abstractDownload PDF (614K) -
Tomohiko YAMAGUCHI, Akihiko KAWAHARA, Tomoki TAIRA, Hideyuki ABE, Yosh ...2012 Volume 51 Issue 4 Pages 267-274
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSObjective : We determined whether GLUT-1 is associated with HIF-1α, CD34-positive microvessel density (MVD), and clinicopathological findings in endometrial adenocarcinomas (EMAC), and whether GLUT-1 is a more useful marker for EMAC Grade 1 diagnosis than endometrial hyperplasia (EH).
Study Design : We performed immunohistochemistry for GLUT-1, HIF-1α, and CD34 in 34 resected EMAC samples and immunohisto/cytochemistry for GLUT-1 in 29 biopsy samples including curettage and cytology in EH and EMAC Grade 1. GLUT-1 expression levels were semiquantitatively evaluated using both the proportion and intensity score.
Results : GLUT-1 expression was found in 53% (9/17) of Grade 1, 91% (10/11) of Grade 2, and 83% (5/6) of Grade 3 resected EMAC samples. GLUT-1 expression was significantly associated with HIF-1α expression and tumor size (P<0.05). GLUT-1 expression was found in no (0/14) EH and 53% (8/15) of EMAC Grade 1 biopsy samples. GLUT-1 expression was found in 7% (1/14) of EH and 60% (9/15) of EMAC Grade 1 cytological samples. GLUT-1 expression differed significantly between EH and EMAC Grade 1 (P<0.05).
Conclusion : GLUT-1 expression appears useful for differential by diagnosing EMAC and EH, and GLUT-1 can be applied to endometrial cytology.View full abstractDownload PDF (1111K) -
Mitsuko TACHIBANA, Mikiko YOSHIDA, Yoko OHKI, Yuko KUROKAWA, Atsuko SO ...2012 Volume 51 Issue 4 Pages 275-280
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSObjective : The cytological differences were studied between HPV positive and negative patients, diagnosed as having atypical squamous cells of undetermined significance (ASC-US) of the uterine cervix, with the HPV-DNA test.
Study Design : Fifty-one HPV positive and 66 negative ASC-US specimens were contrasted and analyzed regarding nuclear and cytoplasmic changes.
Results : 43.6% of the ASC-US were positive and 56.4% negative in the HPV-DNA test. Most of the HPV negative specimens belonged to mild atypia of the early metaplastic and non-specific chronic infection types. Prominent findings found in HPV positive cases were nuclear enlargement, koilocytosis and partial thickening of the nuclear margin.
Bi- and multinuclei with koilocytosis were also noticed more in HPV positive cases.
Conclusion : These differentional points will contribute to the correct cytological diagnosis and minimization in cases of ASC-US.View full abstractDownload PDF (662K)
Clinical Articles
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Mitsumasa OSAKABE, Yu SUZUKI, Yuki SAITO, Miyuki UEMATSU, Gen TAMURA2012 Volume 51 Issue 4 Pages 281-285
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSBackground : Diagnostic confirmation and subtyping of malignant lymphomas requires chromosome banding and flow cytometry in addition to histologic diagnosis. In pulmonary malignant lymphoma, because histologic diagnosis often requires an open lung biopsy, fresh specimens for chromosome banding and flow cytometry cannot be easily obtained.
Case : The patient was a 42-year-old man who was found to have a nodular shadow on chest radiography. Pulmonary adenocarcinoma was suspected on computed tomography, but a transbronchial biopsy and cytology were negative. An open lung biopsy with intraoperative pathological consultation was performed. Histologic diagnosis on frozen sections was difficult, because there were freezing artifacts. Instead, the diagnosis of lymphoma was obtained by intraoperative imprint cytology.
Conclusion : Imprint cytology during intraoperative pathological consultation may help with lymphoma diagnosis.View full abstractDownload PDF (1008K) -
Hiroki FUJINAKA, Michiyo MATSUI, Reiko SHIMOYAMA, Shiho SASAKI, Kazuyo ...2012 Volume 51 Issue 4 Pages 286-289
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSBackground : Secretory carcinoma of the breast is rare and the prognosis good, making it advantageous to confirm its possibility preoperatively by in fine-needle aspiration (FNA) cytology. We focus on the important cytologic findings involved.
Case1 : A woman in her 30s was found in FNA cytology to have numerous in a right breast mass. Tumor cells were arranged in clusters, irregular sheets, mucous globular structures (MGS), or acinar structures. Tumor cells had mildly atypic nuclei of varying sizes. Mitotic activity was low. The nucleocytoplasmic ratio was low because of abundant foamy cytoplasm. A few tumor cells showed a signet ring cell appearance. Resected tumor results yielded a histological diagnosis of secretory carcinoma.
Case2 : A woman in her 40s was found in FNA to have numerous tumor cells in a left breast mass. Cells were rich in cytoplasm with intracytoplasmic lumina (ICL). Signet ring cells and tumor cells with MGS were also seen, as were grape-like configurations of MGS. The histological diagnosis of the resected tumor was secretory carcinoma.
Conclusion : It is important in using FNA cytology determining a diagnosis of mammary secretory carcinoma to find MGS, signet ring cells, or ICL.View full abstractDownload PDF (548K) -
Kiyofumi UEDA, Masatomo KIMURA, Takaaki CHIKUGO, Chikoto DOBASHI, Tada ...2012 Volume 51 Issue 4 Pages 290-294
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSBackground : Allergic fungal rhinosinusitis (AFRS) is considered a highly recurrent allergic refractory rhinosinusitis reaction due to fungi. AFRS is characterized by contents called allergic mucin retained in the paranasal sinuses. Allergic mucin contains a few fungi but no fungal invasion of the sinus mucosa. AFRS is considered relatively rare in Japan. We report a case of AFRS and cytological smear features of paranasal sinus contents.
Case : A 42-year-old woman diagnosed with recurrent bilateral pansinusitis underwent endoscopic sinus surgery. Histological examination of the peanut-butter-like paranasal sinus contents showed allergic mucin containing scattered fungal hyphae. A week later, the smear cytology of similar contents leaking from the frontal sinus into the nasal cavity showed allergic mucin with a few scattered fungal hyphae.
Conclusion : It is important to detect allergic mucin in AFRS on cytodiagnosis of sinus contents. Fungal elements should be looked for in allergic mucin. Cytology is superior to histopathology in detecting and showing the precise morphology of fungi because of the thickness of specimens.View full abstractDownload PDF (839K) -
Akiko KAGOTANI, Mitsuaki ISHIDA, Keiko YOSHIDA, Muneo IWAI, Yoshimitsu ...2012 Volume 51 Issue 4 Pages 295-298
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSBackground : Nested variant of urothelial carcinoma (NV-UC) is an extremely rare histopathological entity of urothelial carcinoma with a poor prognosis. It is characterized by bland histological features resembling von Brunn’s nests. We report a case of NV-UC with an emphasis on the cytological findings of NV-UC of the urinary bladder.
Case : A 70-year-old man was pointed out irregular thickening of the urinary bladder wall on ultrasound sonography and computed tomography. Urine specimen through catheterization revealed small clusters of round to polygonal urothelial cells with mild increase of nuclear/cytoplasmic ratio, coarse chromatin pattern, irregular nuclear membrane, and occasional prominent nucleoli. Although the nuclear size of these cells are not so large, the distance between each tumor cell within the cluster was uneven. On the basis of these cytological features, urothelial carcinoma was suspected.
The biopsy and the subsequent radical prostatocystectomy were performed. And, their histopathological examinations were in accordance with pure form of NV-UC, extending down to the adventitia and the prostatic stroma.
Conclusion : Although the nuclear features of NV-UC are subtle, careful observation of clusters of slightly larger cells in irregular nuclear distribution and abnormal nuclear findings are useful for suggesting NV-UC.View full abstractDownload PDF (499K) -
Yutaka TORII, Kiyoshi HASEGAWA, Kunimi ISHIKAWA, Shuko OE, Rina KATO, ...2012 Volume 51 Issue 4 Pages 299-304
Published: 2012
Released on J-STAGE: October 09, 2012
JOURNAL FREE ACCESSBackground : Primary ovarian squamous cell carcinoma (SCC) is extremely rare, arising in mature cystic teratoma, Brenner tumor, or endometriosis. We report a case of primary ovarian SCC possibly associated with endometriosis.
Case : A 51-year-old woman, 2 gravida, referred to our hospital, was found in imaging results to have an ovarian tumor with cystic and solid components. The diagnosis of right ovarian SCC from intraoperative frozen section necessitated radical surgery. The woman has remained well without evident recurrence in the 18months since completing surgery and chemotherapy.
Tumor imprint cytology showed atypical spherical to oval squamous cells with light green or eosinophilic cytoplasm, hyperchromasia, and slight nuclear atypia. Histopathologically, the tumor diagnosis was highly differentiated keratinizing squamous cell carcinoma, possibly associated with endometriosis, due to marked tumor-wall metaplasia despite no apparent transition from benign endometriosis to carcinoma.
Conclusions : Primary ovarian SCC, if advanced, promises a poor prognosis, so our case was found early. We found tumor imprint cytology to be useful in diagnosis.View full abstractDownload PDF (974K)
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