The aim of the present study was to obtain the optimal immunocytochemical staining condition for a CK5/14+p63+CK7/18 cocktail antibody. Fine needle cytology smear slides from surgically resected breast nodules were used. Immunocytochemical slides were compared under four different antigen retrieval conditions. Twenty minutes with pH9 retrieval produced good staining for the CK5/14+p63+CK7/18 cocktail antibody. Only a few slides are available for breast cytology in routine practice. Multicolor immunocytochemistry using a cocktail antibody is becoming an important ancillary method.
We describe the multiplex immunostaining method, involving the use of a cocktail of four antibodies, which is effective for the diagnosis of non-small cell lung cancer. In our examination, the staining showed 97% sensitivity and 100% specificity for the diagnosis of adenocarcinoma, and 100% sensitivity and specificity for the diagnosis of squamous cell carcinoma.
We report herein on a case of prostatic duct adenocarcinoma. An 84-year-old man was admitted to our hospital with dysuria and macroscopic hematuria. Cystoscopy of the lower urinary tract showed a papillary tumor in the prostatic urethra. In the voided urine cytology, tumor cells with a relatively high N/C ratio and hyperchromasia of the nucleus showed numerous irregular-sized aggregations. Histologically, the adenocarcinoma consisted of tall columnar tumor cells and a papillary pattern was seen. Immunohistochemically, the tumor cells were positive for PSA. Differentiation between prostatic duct adenocarcinoma and urothelial carcinoma depends on identification of the cellular columnar features and immunohistochemistry.
We present a case of a 40-year-old woman with villoglandular-type mucinous adenocarcinoma of the uterine cervix. Large-sized, tight papillary or glandular clusters were observed in the uterine cervical smear, without a necrotic background. Columnar tumor cells stratified with feathery edges showing weak nuclear atypia were observed, with monotonous round to oval nuclei and fine granular chromatin. These findings are consistent with the diagnosis of mucinous adenocarcinoma, villoglandular type. In the presence of weak nuclear atypia, the characteristic structure of villous or papillary clusters in a clear background would be an important clue for the diagnosis of this tumor.