Cause of unsatisfactory preparation in the field of gastric cytology are studied from many elements. Most important elements are, nature of cell materi als, centrifuging technics, technic for smear method, fixation technics, fixative solutions, and duration of fixation etc.
i. If washing material contains a great deal of mucin, pumping is needed to solve mucin, and then, collection of cells are taken from median layer of tube, and smear will be made up in X-style (see photo.).
ii. Bloody material have to be centrifuged in high speed and to be collected from ober layer, if centrifugation is done in low speed, collection of cells must be taken from lower layer, Smear me thod needs W-style (see photo.).
iii. If material contains bile, all handling have to be carried as possible as rapid.
iv. If material contains a great deal of food sedimeut, material will be filtered by gauze, and then collection of cells have to be taken from ober layer of tube
v. Hyperaciditic material must be handled as possible as rapid.
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