In our laboratory, we have studied the morphological changes of various kinds of blood vessels following administration of soluble immune complexes.
After administration of various soluble immune complexs, acute glomerulonephritis in kidney and pulmonary fibrosis occured. In bone and bone marrow, as reported in several previous papers, administration of soluble immune complexes led to general morphological changes in the arterial wall resulting in arteritis. The most characteristic morphological changes seen in the bone marrow were the capillarisation of the sinusoidal endothelia with luminal distension and a severe degree of plasma stasis in the lumina.
With the repeated administration of soluble immune complexes, we observed the formation of micro-abscesses which might developed into osteomyelitis.
In this paper, the formative processes of osteomyelitis will be discussed in relation to Aschoff's definition of inflammation. We will emphasize the importance of the capillarisation of the sinusoidal endothelia for the formation of osteomyelitis.

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A case of 44-year-old female with post-transfusion purpura which was first described as a severe megakaryocytic thrombocytopenic purpura by Schulman in 1961 was reported. The patient who experienced six times pregnancies, had not taken any drugs and not previously received blood transfusion until present time in her medical history. By the reason of the existence of anemia due to myoma uteri, she received the amounts of 1, 000ml of blood transfusion at the period of five days. One hour after the final blood transfusion, nasal and gingival bleeding and generalized purpura developed.
The laboratory examination revealed severe thrombocytopenia, low complement hemolytic activity and antiplatelet isoantibody by the method of complement fixation and agglutination.
It is interested that anti HL-A 9 antibody was detected in her serum. After 25 hospital days, the thrombocytopenic purpura disappeared and now she is healthy with a normal platelet count.
The relation between antiplatelet isoantibody and histocompatible antigen, and the mechanism of the destruction of platelet by antibody and complement was discussed.

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A simple method for the rapid isolation of leukocytes in high yield from small volumes of human whole blood was examined.
The procedures are as follows:
1) Methylcellulose as suspending medium was dissolved in 0.9per cent NaCl in 0.5per cent concentration immediately prior to use.
2) One or two milliliter of heparinized human blood was drawn into 0.5 or 1 milliliter of methylcellulose solution.
3) Sample in the tube with inner diameter of 7mm was mixed gently and allowed to stand for 10 to 30 minutes.
4) The upper white cells rich portion of plasma layer was collected into another tube.
5) The tube was then centrifuged at 450g for 15 minutes and the supernate was removed by aspiration.
6) Small quantities of 0.9per cent NaCl was added to the remaining white cell pellets. Mixed gently, and centrifuged at 200g for 10 minutes.
7) These processes were repeated two to three times.
8) The final white cell pellets were resuspended in autoserum or saline for different examinations.
Yield of leukocytes recovered in the aspirated supernate were calculated as the percent of the total number of leukocytes present in the original whole blood sample.
To investigate the normality of isolated leukocytes, the appearance of the cells, alkaline phosphatase, acid phosphatase, glucose 6 phosphate dehydrogenase in neutrophilic leukocytes on the smear, lysozyme activities in neutrophilic leukocytes and monocytes with nephelometry, NBT test and phogocytic activity in neutrophilic leukocytes were examined.
The results of investigation are as follows:
1) Leukocyte yield (± standard deviation) obtained using 0.5per cent methylcellulose was 84.5±3.8per cent at 20 minutes sedimentation time. The proportion of erythrocytes and leukocytes in the aspirated supernate was 3.3±0.6. This method has proved excellent yield, as compared to yield of 3per cent dextran method in 70per cent or so.
2) Two per cent methylcellulose-six per cent dextran mixed system is appliciable to rapid isolation, which is estimated about 70per cent at 10 minutes separation time.
3) The observation of stained preparation of leukocytes isolated using methylcellulose by optic or phase microscope ware not shown any abnormlity in shape.
4) Differential counts (per cent) of 500 cells in each smear of leukocytes, from whole blood and in the plasma layer after separation were similar to each other.
5) Enzyme activities in leukocytes did not differ significantly before and after separation.
6) The results of functional studies in leukocytes indicated that the possitive index of phagocytosis in neutrophilic leukocytes increased slightly following the separation. But no statistical differ is recognized.

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Temporary arrestation of aortic circulation is necessary in surgical correction of coarctation of the aorta or aortic aneurysm. As is well known, however, there occurs sevier hypotension (also called “declamping shock”) when the clamps are removed, and a marked hemorrhagic tendency frequently acompanies the sudden hypotension for an unknown cause. The work presented here is a study to investigate into possible causes of the hemorrhagic tendency which often follows aortic cross-clamping.
Materials and Methods
Forty-five adult mongrel dogs weighing between 10 and 15kg were anesthetized with 30mg/kg of penthobarbital sodium and placed on controlled respiration by means of an endotracheal tube. The descending thoracic aorta was expoged through a left intercostal incision and clamped, extending downward for about 5cm from the first part of the left subclavian artery. Aortic circulation was arrested for 90 minutes. Blood samples were obtained from a siliconized polyethylene catherter placed in the left carotid artery to carry out blood test with respect to acid-bace balance, platelet count, fibrinogen, prothrombin time, PTT, coagulation factors V and VIII, FDP and fibrinolytic activity. After declamping, several organs were examined under the microscope.
Result and Discussion
After declamping, acidosis gradually developed, and platelets, fibrinogen levels and other coagulation factors began to decrease about 30 minutes after declamping. Secondary fibrinolysis was accelerated so that FDP increased. As coagulation factors were consumed an account of intravascular coagulation following declamping, hemorrhagic tendency appeared. Microscopic examination revealed microthromboses in such organs as the lungs, kidneys and small intestine. There findings suggested that consumption coagulopathy arising from disseminated intravascular coagulation was responsible for the marked hemorrhagic tendency observed of terr aortic cross-clamping. Although the mechanisms of this intravascular coagulation have not been elucidated, it seems beyond doubt that slow capillary blood flow and devlopment of acidosis predispoes to the formation of clots in the capillaries. acidosis predispoes to the formation of clots in the capillaries. It also may be inferred from the results of this study that tissue coagulation factors are activated with increasing vascular permeability due to slow capillary blood flow, predisposing to intravascular coagulation. As contusion, it may be said that a temporary extracorporeal bypass of the aorta affords an effective means to prevent DIC arising from slow capillary blood flow.

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