巻号一覧
19 巻, 4-6 号
選択された号の論文の11件中1～11を表示しています
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斎藤 七五三男, 佐々木 武道, 菊地 さと子, 山下 寛, 猪股 みな子, 真弓 忠1973 年 19 巻 4-6 号 p. 107-111
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーDetection of Australia-antigen and anti-Au antibody was performed by Immune Adherence Hemagglutination and passive hemagglutination technique.
Positive rates of Au-antigen and antibody were 2.5% and 21% respectirely. Higher frequency of Au-antigen was observed in younger age group than in older age group. Positive rate of Au-antigen and antibody in blood group O donors was higher than in other ABO Blood group donors.抄録全体を表示PDF形式でダウンロード (542K) -
佐久総合病院における実態藤田 公生, 寺島 重信, 渡辺 喜一郎1973 年 19 巻 4-6 号 p. 112-115
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーOne-hundred and four patients operated at Saku Central Hospital between September 1971 and January 1972 were followed; hepatitis B antigen with immuneadherence hemagglutination technique, hepatitis B antibody with passive hemagglutination technique, and transaminases.
Antigen incidence were 0.96 per cent in the patients and 1.62 per cent in blood donors. Among 9 recipients of hepatitis B antigen positive blood, 3 suffered from hepatitis (and one died with antigenemia). Another 4 patients developed hepatitis B antibody. The remaining 2 patients showed transient elevation of transaminases on the next day.
In the remaining 95 patients, I was antigen carrier. Four developed hepatitis, one of them became hepatitis B antigen carrier afterwards. Eight postoperatively developed antibody, which could not be detected preoperatively.
All 13 patients detected antibody preoperatively, were uneventful except one of the antigen positive blood transfused patients, whose serum glutamic oxaloacetic transaminase elevated to 152 units.抄録全体を表示PDF形式でダウンロード (425K) -
松倉 寛1973 年 19 巻 4-6 号 p. 116-123
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーUsing immuno-electron microscopical method, the localization of the A antigen sites on human erythrocytes was demonstrated.
It was shown that the A antigen sites are evenly distributed on a red cell. They are so numerous that no space seems to remain between adjacent antigen sites. In the examination using the diluted conjugates, however, the distribution of ferritin particles on the cells was diffuse and random. This suggests that the individual antigen sites on a cell were not equally capable to bind antibody.
In the experiments on the subgroup or variant, it was noted that A2 cells have fewer than have A1 cells and Ax cells have fewer than have A2 cells, when A1 cells were compared with A2 or Ax cells in the degree of the A antigen sites, and that the attached ferritin particles indicating the A site positions conspicuously varied in number from cell to cell in the same specimen. This indicate that the different serological behaviour of A1, A2 and Ax cells in tests with anti-A reagents are due to both of a quantitative and a qualitative difference.
In the umbilical cord blood, red cells in the same specimen were divided into four populations according to the number of the A antigen sites on the cell. It was perceived that the specimen from umbilical cord consists in the following ratio of red cell numbers in each population; I (possessing A antigen sites of about 5×105 or more) 18%, II (possessing 2×105-4×105 sites) 29%, III (possessing the sites of about 105) 28%, IV (possessing 0-104 sites) 25%.抄録全体を表示PDF形式でダウンロード (9730K) -
血清肝炎ワクチンの可能性隅田 幸男1973 年 19 巻 4-6 号 p. 124-126
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーPDF形式でダウンロード (408K) -
小暮 正久1973 年 19 巻 4-6 号 p. 127-134
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーPleurotus ostreatus and Pleurotus spodoleucus extracts, after heating at 56°C for 30 minutes, agglutinate O red cells most strongly and AB red cells most weakly. The activity of Pleurotus extract, present in the fraction precipitated at 0.3 and 0.6 saturation with (NH4)2 SO4, is inhibited by O secretor saliva and Shigella dyselteriae polysaccharide, but not by O non-secretor saliva and Type XIV Pneumococcus polysaccharide.
As the result of hemagglutination inhibition test with sugars, these Pleurotus lectins are inhibited by 2′-fucosyllactose most strongly, D-galactose and lactose more strongly than melibiose, D-galactosamine and L-rhamnose, but not inhibited by L-fucose, D-glucose, D-mannose and D-glucosamine.
Treatment of group O red cells and O secretor saliva with H-decomposing enzyme (α-L-fucosidase) from Bacillus fulminans resulted in the complete loss of reactivity with both anti-H eel serum and pleurotus ostreatus lectin.
Pleurotus ostreatus and Pleurotus spodoleucus lectins react specificially with H substance (O substance and Eisler-Kagaya's heterophile anti-gen) and are assumed to be analogous to anti-Shigella dysenteriae immune chicken serum and Laburnum alpinum lectin.抄録全体を表示PDF形式でダウンロード (1074K) -
滝本 義一1973 年 19 巻 4-6 号 p. 135-141
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーUsing tannic acid or bis-diazotized benzidine, Australia antigen was coated to glutaraldehyde-fixed human erythrocytes. The observed titers were comparable to those obtained with sheep erythrocytes, but clear cut pictures of negative hemagglutination were rot obtained in human erythrocytes. The indistinctness of settling patterns was not improved by decreasing of Au antigen or coupling reagents concentration, and also not by the addition of Gum arabic or Tween 80 to the agglutination medium. The advantages were, however, recognized that for the detection of anti-Au antigen in human sera the sensitized human erythrocytes were able to avoid difficulties caused by the presence in the sera being examined of hemagglutinins againt non-homologous erythrocytes and the test was read more rapidly in human cells than in sheep cells because of rapid production of settling patterns.抄録全体を表示PDF形式でダウンロード (2232K) -
基準改訂における問題点の検討尾崎 順一, 安田 純一1973 年 19 巻 4-6 号 p. 142-151
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーTechnics in potency test of albumin agglutinating anti-D blood-typing serum were thoroughly studied. From the results obtained, the following points should be considered in formulation of the “Minimum Requirements for Anti-D Blood-Typing Serum of Human Origin” which will be to replace the currently effective “Minimum Requirements for Anti-Rh Typing Serum-Human Origin” promulgated in 1959.
1) In agglutinin titration, group AB serum should be used as the diluent of anti-D serum, while red cells should be suspended in 20% bovine albumin.
2) Minimum agglutinin titre required should be elevated to 1: 256 or higher.
3) In avidity test, 6-8% bovine albumin can be used as suspending medium of red cells, although it is less active than group AB serum.
There seems some alteration necessary in the potency test of Coombs serum, namely in the expression of the strength of sensitization of red cells with anti-D serum, which will exhibit higher titre in the revised technic than in conventional method.抄録全体を表示PDF形式でダウンロード (1241K) -
佐治 博夫, 宮島 淳子, 細井 武光, 矢原 靖司1973 年 19 巻 4-6 号 p. 152-157
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーThe immunoelectrosyneresis inhibition method (ESI) was devised at our laboratory. The procedure is based on the principle that the conventional electrosyneresis (ES) is carried out after the HB antigen might be contained in specimen serum absorbs the HB antibody contained in anti-HB serum reagent. The anti-HB serum reagent has a titer of 1:16 against standard HB antigen reagent by the ES. One hundred μl of the specimen serum is mixed with 10μl of the anti-HB serum and incubated for 30 minutes at 37°C. Then the mixture is used for the ES as the antiserum against the standard HB antigen reagent which has a titer of 1:8 by the ES method, diluted from single donor serum contained high titer HB antigen. The absence of a pretipitation line indicates that HB antigen is present in the specimen serum.
This method is easy to perform the HB antigen screening and overcomes the problem of the prozone phenomenon due to antigen excess. This is more sensitive than the conventional ES, and has ability to detect as many HB antigen as the complement fixation method (CF) does. And also, this method can eliminate the false positive reaction by the CF. The precipitation lines of this method always sharp and clear and the identification of HB antigen in the specimen is accurately performed.
It is suggested that ESI may be used together with the other method for the blood donor screening from a viewpoint of double check.抄録全体を表示PDF形式でダウンロード (664K) -
村上 省三, 川井 成子, 堀越 享1973 年 19 巻 4-6 号 p. 158-160
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーWe found a new antigen-antibody system which is associated with but distinct from the HB Ag-Ab system. Our new system is perhaps very similar or identical with the e-system reported by Magnius. And the antigen is found more frequently in patient sera than in donor sera, but as for the antibody the reverse is the case. It is interesting that in Japan the antigen is also found in some donor sera.抄録全体を表示PDF形式でダウンロード (332K) -
小暮 正久1973 年 19 巻 4-6 号 p. 161-166
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーWhen group O red cells were incubated with UDP-Galactose and serum from group B or AB subjects, they became strongly agglutinable by human anti-B serum. Agglutination titers of these converted red cells against human anti-B serum (1:512) were 1:128.
Group O red cells incubated with UDP-Galactose and serum from group Bm or A1Bm subjects were converted to B active cells. Agglutination titers of these converted red cells against human anti-B serum (1:512) were 1:8 or 1:16.
Comparison of the agglutination titers of the converted cells against human anti-B serum shows that the activity of serum α-galactosyltransferase from group Bm subjects is approximately one-eighth or one-sixteenth of that from group B subjects.
When group O red cells were incubated with UDP-Galactose and serum from group Cis AB subjects, they could not be agglutinated by human anti-B serum. The activity of serum α-galactosyltransferase from group Cis AB subjects could not be demonstrable by agglutination test. This is considered to be associated with the regularly present cold anti-B agglutinins in the sera of group Cis AB subjects.抄録全体を表示PDF形式でダウンロード (839K) -
1973 年 19 巻 4-6 号 p. 167-173
発行日: 1973年
公開日: 2010/03/12
ジャーナル フリーPDF形式でダウンロード (813K)
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