巻号一覧
30 巻, 2 号
選択された号の論文の8件中1～8を表示しています
- |<
- <
- 1
- >
- >|
-
中尾 昌史, 中川 治, 植原 善彦, 仲西 郁雄, 吉岡 章, 福井 弘1984 年 30 巻 2 号 p. 99-102
発行日: 1984年
公開日: 2010/03/12
ジャーナル フリーAnti-A anti-B hemoagglutinin titers in factor VIII concentrates (F. VIII Conc.) and prothrombin complex concentrates (PCC) were determined by both the saline and antihuman globulin sera (Coombs) techniques.
All of the 4 commercial factor VIII concentrates contained measurable titers of anti-A and anti-B isoagglutinins ranging from 1:4 to 1:32 for anti-A and 1:2 to 1:32 for anti-B. The “immune” anti-A and anti-B titers determined by Coombs technique contained the mixture of IgG and IgM alloantibodies ranging from 1:8 to 1:128 for anti-A and from 1:16 to 1:64 for anti-B. In preparations from blood group A or B only anti-B or -A was detected, respectively.
The anti-A and anti-B were also detected in PCC or APCC and the titers were lower than those of F. VIII Conc.. And neither anti-A nor anti-B was detected in Proplex and Autoplex.
There were some differences among these preparations or lots, therefore, indication of the titers on the label is preferable.抄録全体を表示PDF形式でダウンロード (481K) -
吉岡 章, 塚田 周平, 嶋 緑倫, 植原 善彦, 中尾 昌史, 中川 治1984 年 30 巻 2 号 p. 103-107
発行日: 1984年
公開日: 2010/03/12
ジャーナル フリーFactor VIII clotting antigen (VIIICAg) and VIIICAg-phospholipid (PL) complexes in standard or activated prothrombin complex concentrates were determined using a two-site solid phase immunoradiometric assay (IRMA) for VIIICAg.
All of the 3 commercial PCCs (Proplex, PPSB, Konyne) and the 2 APCCs (Autoplex, FEIBA) contained measurable amount of VIIICAg (6.2-389U/dl). Then, the action of phospholipase-C (PL-C) on VIIICAg-PL complexes in normal plasma, cryoprecipitate, factor VIII concentrate or (A) PCC was investigated, with enzyme activity monitored by thin layer chromatography. Treatment of factor VIII fractions with PL-C (5u/u VIIICAg) resulted in 25-30% increases in VIII fractions, while (A)PCC showed 25-80% increase, indicating VIIICAg-PL complex present to a greater extent than in factor VIII fractions. The presence of VIIICAg-PL complexes in (A)PCC may thus lead to the protection of VIIIC from inactivation by antibodies and could have important clinical application in treatment of hemophiliasc with inhibitor.抄録全体を表示PDF形式でダウンロード (618K) -
井上 香代, 松田 利夫1984 年 30 巻 2 号 p. 108-113
発行日: 1984年
公開日: 2010/03/12
ジャーナル フリーRecent investigation by Kang (1980) suggested that the recovery of factor VIII activity was remarkably increased in cryoprecipitate prepared by the modified thaw-siphon (MTS) method. In the present study, cryoprecipitate was prepared by the MTS method and compared with slow-thaw and rapid-thaw methods. Assays for factors II, V, VIII, IX and XIII, von Willebrand factor, fibrinogen, total protein and albumin were performed on cryoprecipitates and on supernatant plasmas. The stability of factor VIII activity in cryoprecipitates after thawing was observed during incubations at 4°, 22° and 37°C for 24 hours. Relative viscosities were also determined on cryoprecipitate preparations.
Recoveries of factor VIII activity in cryoprecipitate were obtained 46.0±4.5% by slow-thaw, 64.1±4.1% by rapid-thaw and 72.0±5.1% by MTS method. The results indicated that the recovery of factor VIII activity in MTS cryoprecipitate was more than 100 units from 180ml of fresh frozen plasma, while recoveries of other plasma proteins, particularly fibrinogen, are reduced. Therefore the relative viscosity of MTS cryoprecipitate preparations were also less than slow-thaw preparations. However factor VIII activity in MTS cryoprecipitate preparations after thawing was less stable than slow-thaw preparations. In MTS supernatant plasma, there was little loss of plasma proteins except factor VIII, fibrinogen and von Willebrand factor.抄録全体を表示PDF形式でダウンロード (648K) -
三浦 泰裕1984 年 30 巻 2 号 p. 114-120
発行日: 1984年
公開日: 2010/03/12
ジャーナル フリーActivated-type coagulation factor preparations such as FEIBA or AUTOPLEX exhibited by themselves activities higher than that of Factor VIII concentrates in the system of potency estimation of the latter (activated partial thromboplastin time assay).
Furthermore, they were found to have shown a potentiating effect on the Factor VIII activity of Factor VIII concentrates or of normal human plasma to which they were added, in contrast to the normal Factor IX preparations which were devoid of such “potentiating” effect. This finding may be useful in differentiating the former preparations from the latter. Nevertheless, this finding was not always in agreement with the results of other test procedures which had been devised for the detection of activated clotting factors. Therefore, the activated clotting factors detected by each of the various test methods do not seem to be identical.
From these results, it would be recommended to revise our Minimum Requirements for Biological Products in respect to more reasonable delineation of activated-type coagulation factor preparations from normal Factor VIII or IX concentrates.抄録全体を表示PDF形式でダウンロード (890K) -
劉 景漢, 韓 玉鳳, 向 文玉, 陳 晋云, 干 國, 李 輝, De-Kai She, Xiu Zhao1984 年 30 巻 2 号 p. 121-125
発行日: 1984年
公開日: 2010/03/12
ジャーナル フリーIt has been well established that adenosine phosphate added into the storage fluid may prolong the storage period of the blood. However, this agent may exert some toxic effects, which requires careful study. In order to elucidate these effects and to maintain safety in practical use of this agent, we have observed, using white mice as the experimental animals, its deleterious effects on the internal organs and their functions. The results of the experiments indicated that this agent caused damage not only on the kidneys but also on various other internal organs such as liver, spleen and thymus etc. The liver and kidneys were more seriously injured. When it was used in the dosage of 10mg/kg, it revealed relatively mild histopathological changes in the internal organs in a part of the experimental animals. However, when the dosage was increased to 200-300mg/kg the experimental animals showed reduction in body weight, changes in the weight of different organs with serious histopathological changes, particularly when 300mg/kg was used. Simultaneously we observed the alteration of functions of these affected organs and the blood as well. Evidently the toxicity of APO4 is systemic and related to the dosage of the preparation used.抄録全体を表示PDF形式でダウンロード (580K) -
劉 景漢, 韓 玉鳳, 向 文玉, 陳 晋云, 干 國, 李 輝, De-Kai She, Xiu Zhao1984 年 30 巻 2 号 p. 126-129
発行日: 1984年
公開日: 2010/03/12
ジャーナル フリーThe additive into the blood as an energy supporter, Adenine phosphate (APO4) can extend the storage life of banked blood. But the usefulness of transfusing this supplemented blood depends on safety of the chemical. Thus the toxicity of APO4 was studied experimentally in dogs. The animals receiving 10mg/kg APO4 intravenously showed no difference from the control group injected with normal saline. A dose of 30mg/kg produced slight rise of GPT and BUN, as well as mild or moderate histological changes, which returned to normal range after the stop of the medication. On the other hand, the dogs taking 90mg/kg of APO4 suffered from severe renal and hepatic failure, with marked elevation of GPT and BUN values, liver parenchymatous degeneration, necrosis and casts in urine. These disorders persisted and some pathological changes were present also in spleen and thymus suggesting depression on immunity. In conclusion, the dose of 10mg/kg can be considered probably the safety threshold for man. This has been proved clinically by pratical transfusion in recent years. Nevertheless, the potential toxicity of APO4 is still worthy on further investigation.抄録全体を表示PDF形式でダウンロード (409K) -
毛利 博, 萩原 昭二, 森 啓, 松野 一彦, 新倉 春男, 寺田 秀夫1984 年 30 巻 2 号 p. 130-133
発行日: 1984年
公開日: 2010/03/12
ジャーナル フリーContinuous plasmapheresis with Hemonetics Model 30 showed to be a successful therapy in a 40 year-old-male with thyroid storm. Serum thyroxine (T4), triiodothyronine (T3) and free T4 were raised (T4 25.5μg/dl, T3 436ng/dl, free T4>15ng/dl) before plasmapheresis. The patient's mental status showed marked improvement before the completion of plasmapheresis whereby 2.4 liters of plasma were exchanged within 4 hours. Although 283.2μg T4 and 5.0μg T3 were removed, these serum values remained unchanged probably due to the reflux of thyroid hormones from tissue into intravascular space. But a significant fall in free T4 level was observed.
So it was concluded that plasmapheresis was very effective to thyroid storm because of not only removal of a great amount_of thyroid hormone, but provision of sufficiet donor's protein binding to the free, metabolically active portions of patient's serum T3 and T4.抄録全体を表示PDF形式でダウンロード (520K) -
菊地 正輝, 遠藤 信義, 瀬尾 たい子, 大久保 康人, 山口 英夫1984 年 30 巻 2 号 p. 134-136
発行日: 1984年
公開日: 2010/03/12
ジャーナル フリーA sample of red cells of a patient suffering from carcinomotous peritonitis was found to be incompatible with 9 of the 17 donors' plasmas in minor crossmatch tests. Direct antiglobulin test was negative. Moreover, the patient's red cells reacted with 72 of the 150 adult AB sera, while they failed to react with following lectins: Salvia horminum, Salvia sclarea turkestanica and Dolichos biflorus absorbed with A1 cells. After acetylation of the red cells, their reactivity with AB sera disappeared, but the A and B antigenicity did not vary. The results obtained strongly suggested that this group AB patient developed the acquired B antigen.抄録全体を表示PDF形式でダウンロード (409K)
- |<
- <
- 1
- >
- >|