日本輸血学会雑誌
Online ISSN : 1883-8383
Print ISSN : 0546-1448
ISSN-L : 0546-1448
33 巻, 6 号
選択された号の論文の15件中1~15を表示しています
  • 赤血球洗浄利用率, 混入細菌除去に関する検討
    福井 明, 松本 信夫, 高折 益彦
    1987 年 33 巻 6 号 p. 683-688
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    Several trials were carried out in 60 cases with intraoperative autotransfusion.
    In order to minimize hemolysis and bacterial contamination in red cell suspension which was reconstructed from salvaged blood.
    Surface activating agent, poloxamer 188, added in the aspiration fluid, had no protective effect for hemolysis.
    The improvement of aspirator, that was able to mix with the aspiration fluid at the top and was able to minimize obstruction by the tissue fragment in the lumen, seemed effective to reduce the hemolysis.
    Adding ampicillin sodium of 2g in 1, 000ml of saline for aspiration fluid and furthermore adding either latamoxef sodium of 2g or ceftizoxime sodium 2g in 1, 000ml of saline for the rinsing red cells reduced the bacterial contamination rate from 47.8% to 13.5% significantly.
    In addition, it seemed effective to minimize the bacterial contamination rate in this study that double or triple amount of the rinse solution was used in the process constructing red cell suspension and application of negative pressure in the aspiration system was limited strictly only when hemorrhage occurred in the operation field and the aspiration was needed essentially.
  • 上平 憲, 中嶋 茂宏, 市丸 道人, 親川 幸信, 森内 幸美, 鳥谷 和洋, 樅田 三郎, 池田 柊一, 奥田 浩人, 金村 真智子, ...
    1987 年 33 巻 6 号 p. 689-694
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    It is well known that human T lymphotropic virus type I (HTLV-I) associated adult T cell leukemia (ATL) indirectly leads to some disorders such as monoclonal gammopathies, cancers, pulmonary complications and stronglyoides infections. The causative mechanism may be related with immunological abnormalities induced by HTLV-I.
    Therefore, we studied lymphocyte subsets and immunosuppressive acid-proteins (IAP), especially variations with increasing age, in serologic HTLV-I+/- status of healthy adults.
    We present results from 131 carriers and 74 non carriers.
    1) In all age group, comparison of carriers with non carriers subsets of T3, T4, T8, IL-2R, Leu-7 and Leu-11 bearing lymphocytes are not statistically different. HTLV-I carriers had higher the mean T4:T8 ratio (1.9±1.0vs 1.6±0.5) and lower the T3-mean fluorescent intensity (73±11vs 78±11) than non-carriers.
    2) T4/T8, T3-MFI and IL-2R lymphocytes is significantly different only over the age of 50 years between carriers and non carriers.
    3) Actually only limited carriers with abnormal lymphocytes could be obtained concering these results showed significant differences.
    4) Significant elevation of IAP with increasing age were noted only in carriers.
    5) Titers of antibodies for HTLV-I are unaffected by aging.
  • 田中 光信, 瀬尾 たい子, 山口 英夫, 大久保 康人
    1987 年 33 巻 6 号 p. 695-701
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    Zygosity determinations are important in population and family studies. The purpose of this study is to determine whether an Rh phenotype of red cells is derived from a homozugous or heterozygous subject by the use of the indirect immunoflurorescence Flow Cytometry (SPIII) without family studies.
    As a preliminary study, conditions for the sensitization of red cells with antibodies were investigated. We found purchesed anti-Rh typing sera and mouse monoclonal antibodies prepared in our labortory to be usable in the 8-to 32-fold dilutions.
    The best condition for the primary sensitization of red cells with the sera mentioned above was to incubate them at 37°C for 1 hour and overnight at 4°C.
    The secondary sensitization was carried out with 100-fold diluted FITC labeled antihuman or antimouse globulin at 4°C for 30 mintes. In this sutdy selection of the suitable Rh-antibody was most important.
  • 田中 光信, 瀬尾 たい子, 宮木 千弥子, 山本 和子, 山口 英夫, 大久保 康人, 御手洗 安興, 内山 英一, 宮田 義久
    1987 年 33 巻 6 号 p. 702-709
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    In the preceding paper, We reported preliminaries of the Rh Zygosity determination by the use of the indirect immunofluorescence Flow Cytometry method (FCM).
    This report deals with the Zygosity determination of the rare blood -D- and Rhnull by FCM.
    All cell samples from the parents of the three -D- propositi showed a typically heterozygous pattern for -D- haplotype by FCM.
    As for the O Rhnull family, FCM of W. O.'s parents showed heterozygous patterns for c, E, D and Hro respectively.
    The parents of T. S, another Rhnull showed expected patterns for the Rh haplotypes.
    The results obtained indicated that the propositus of the W. O, Rhnull was due to the amorph Rhnull gene and that of T. S, Rhnull was of the regulator type.
  • 菊地 金男, 正宗 良知
    1987 年 33 巻 6 号 p. 710-715
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    Relation between perioperative blood transfusion and prognosis after surgery for gastric cancer was studied retrospectively on 2086 patients who had undergone histologically curative operation in the 2nd study of Cooperative Study Group of Surgical Adjuvant Chemotherapy for Gastric Cancer. These patients were divided into the 3 groups according to the administraiton of anticancer drugs (A: MMC, B: MMC+FT, C: FT). The survival and recurrence rate of transfused patients were compared with those of non-transfused patients in each histological stage of A, B and C groups.
    Macroscopic factors of recurrence such as lymph nodal metastases, tumor size and serosal invasion in transfused patients were overestimated, and total gastrectomy with splenectomy and wide extirpation of lymph nodes were performed along with blood transfusion. But microscopic factors of recurrence in transfused patients were better than in non-transfused patients.
    Five-year actuarial survival rate in transfused patients was generally lower than in non-transfused patients. Especialy the 5-year survival rates of transfused patients in stage II of A, C groups and stage III of A, B groups were respectively lower than those of non-transfused patients. Five-year actuarial recurrence rate in transfused patients was higher than in non-transfused patients. The 5-year recurrence rates of transfused patients in stage I of A, B groups, stage II of A group and stage III of B group were severally higher than those of non-transfused patients. Those rates were statistically significant.
  • 上平 憲, 中嶋 茂宏, 市丸 道人, 親川 幸信, 鳥谷 和洋, 早田 央, 池田 柊一, 森内 幸美, 縦田 三郎
    1987 年 33 巻 6 号 p. 716-721
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    The relationship between clinico-immunological state and antibody prevalence for Human Immunodeficiency Virus (HIV) and Human T-Lymphotropic Virus type-I (HTLV-I) was examined for 18 hemophiliacs including one patient with von-Willebrand disease in our hospital.
    Seropositivity among hemophiliacs was 53.3% for HIV and 33.3% for HTLV-I. Double infection with HIV and HTLV-I was confirmed in four cases out of 18 cases.
    We could not clarlify whether infection by the proliferative member of HTLV-I or by the cytopathic member of HIV influences subsequent infection by the other subtypes or not. Cases with double infection had been frequently complicated by lymphadenopathy and advanced-immunologic abnormalities comparing with cases with single HIV infection. This findings suggests that double infection probably far more serious consequences than single infection of each virus.
    Therefore, in the Nagasaki district, an ATL endemic area in Japan, we have to prevent the spread of the both HIV and HTLV-I.
  • 大久保 進, 須藤 妙子, 石田 萌子, 安永 幸二郎
    1987 年 33 巻 6 号 p. 722-727
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    Anti-glucocorticoid (GCS) antibody or antibody-like substances were detected in our three patients with malignant lymphoma, SLE, and SLE combined with AIHA, respectively, by means of the hemagglutination test (HA). The incidence of positive HAs among our patients with collagen diseases was as high as 4.5% (2/44 cases). It was possible to neutralize all antibodies by anti-IgM serum, and the corresponding antigen determinant was supposed to be the common structural portion of the GCSs themselves. Since the results of the HA did not always correspond to those of drug-induced lymphocyte stimulation test (DLST) or the patch test, or to such allergic manifestations as urticaria, a plurality in the mechanisms of antibody production and of allergic manifestations against the GCSs was supposed.
  • 浮田 昌彦, 山田 紀子, 森分 智子, 渡辺 文江
    1987 年 33 巻 6 号 p. 728-735
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    The number of pregnant women with anti-D antibodies have been recently decreased due to the routine administration of anti-D immunoglobulin soon after delivery. Screening test even in D-positive pregnant women have been generally tested. All obstetric patients who delivered in our hospital during the past ten years (from April, 1976 to March, 1986) were screened for unexpected antibodies. If an IgG antibody was detected in maternal serum, it was identified and titrated, as well the cord blood of her infant was examined for HDN. The incidence of unexpected antibodies was 2.01% (231 out of 11, 468), which was 8.43% in D-negatives and 1.92% in D-positives. Out of 231 unexpected antibodies there were 50 (21.6%) IgG, 174 (75.3%) IgM and 7 IgG-IgM antibodies. 88% of IgG antibodies could cause HDNs, and all IgG in addition to 35% of IgM antibodies could cause hemolytic transfusion reaction. Out of 41 (82%) IgG antibodies for Rh system, there were 24 anti-E, 14 anti-D and 3 other antibodies. Although the ratio of E-incompatible pregnancies was 32.5%, HDNs requiring exchange transfusion were more in cases with anti-D than those with anti-E. In D-positive pregnant women who received transfusion previously, unexpected antibodies were detected in 3.85%. E-incompatible transfusions were possibly more immunogenic and might cause more severe HDNs. It is recommended that E-incompatible transfusion in childbearing women should be withheld.
  • 坂内 誠, 松田 利夫
    1987 年 33 巻 6 号 p. 736-740
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    Platelet size distribution and aggregation response were studied in platelet concentrates (PCs) containing platelet aggregates. Both normal and abnormal platelet size distribution were observed. As the number of clumps in PC increased, abnormal size distribution patterns also increased. As size distribution became abnormal, aggregability to thrombin decreased. Passage of PC through microaggregate filters did not affect platelet functions.
    These in vitro results indicate that some PCs, containing platelet aggregates can be used for transfusions while others can not. The selection of the proper PC can be judged by the number of clumps, and more exactly, by platelet size distribution. PCs of normal size distribution can be used for transfusion provided adequate blood filters are employed.
  • 大村 孝男, 平尾 豊, 川辺 晴英, 上村 八尋, 西田 正行
    1987 年 33 巻 6 号 p. 741-744
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    Safety of hepatitis B vaccine prepared from HBeAg positive plasma containing large amounts of HBsAg was investigated by following the relative reduction of HBV DNA in the purification steps by means of DNA-DNA hybridization. Two steps in the purification procedure were found to efficiently eliminate HBV DNA in the source material. In total, HBV DNA was calculated to be reduced by a factor of 5×10-9. From these results, it was concluded that large scale production of highly safe hepatitis B vaccine from HBeAg positive plasma was possible.
  • 能勢 義介, 荒木 延夫, 山平 広治, 清水 準也, 佐藤 薫, 中辻 孝子, 辻 公美, 小野 一男
    1987 年 33 巻 6 号 p. 745-749
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    HLA class I monomorphic monoclonal antibody Yu-3-20A, HLA class II monomorphic monoclonal antibody TOK39-4, and HLA-A and -B interlocus monoclonal antibody TOK45-3A were used in the ELISA assay of platelets. We used 9 samples from the A24-negative and Bw4-negative, 15 samples from the A24-positive, 10 samples from the Bw4-positive, and 18 samples from the A24-positive and Bw4-positive individuals. The monoclonal antibody Yu-3-20A showed the identical positive reactivity with all of the platelets used. The monoclonal antibody TOK39-4 showed no reactivity with any of the platelets. The monoclonal antibody TOK45-3A showed positive reaction with an O. D. value of 0.741±0.271 with 25 samples of the platelets possessing either A24 or Bw4. It also showed reaction with an O. D. value of 1.535±0.253 with the platelets possessing both of the A24 and Bw4. This results indicate that the reactivity with HLA-A and -B interlocus monoclonal antibody correlates with the HLA phenotype of platelets.
  • 上村 八尋, 後藤 節, 須山 忠和, 浜本 嘉昭, 原田 信志, 山本 直樹
    1987 年 33 巻 6 号 p. 750-754
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    Plasma or Cohn fraction (Fr) II+III suspension was mixed with 1/40 volume of HTLV-III solution and processed by the Cohn alcohol fractionation and polyethylene glycol (PEG) fractionation methods.
    The spiked HTLV-III can be inactivated by a rate of>104 during the alcohol fractionation step from plasma to Fr-II+III, by>104 from Fr-II+III to Fr-II and by>104 during the PEG fractionation step from Fr-II+III to intravenous IgG (IVIG) fraction.
    The cumulative efficiency of the entire process A: Plasma→Fr-II+III→Fr-I→PEG process→IVIG, or B: Plasma→Fr-II+III→PEG process→IVIG is calculated to correspond to an elimination rate of>1012 or>108, respectively.
  • 阿波屋 典子, 井上 昌子, 虫明 佳子, 清水 浩代, 藤田 往子, 藤川 容子, 金光 靖, 椿 和央, 堀内 篤, 岩永 隆行, 冨田 ...
    1987 年 33 巻 6 号 p. 755-760
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    A 53-year old man without history of prior transfusion, underwent operation for regurgitation of mitral valve and stenosis of coronary artery with 18 units of fresh whole blood transfusion.
    He was blood group B, Rh D (+), and no irregular red cell alloantibodies were revealed with pre-operative routine screening or crossmatching tests. The post-operative course was generally well, but moderate anemia appeared gradually from about 2 weeks after operation, and then hemoglobinuria and severe reticulocytosis were followed at about 1 week later. Moreover laboratory tests showed increase of LDH and indirect bilirubin levels with decreased haptoglobin level.
    Thus delayed hemolytic transfusion reaction was suspected by these clinical or laboratory findings. Potent anti-Hro antibody accompanied with anti-P1 were detected in his serum during rescreening tests, and his own red blood cels did not react completely with the other anti-Hro sera. Such cells showed no exceptional strong D activity comparing with usual D positive red blood cells, therefore it had been really unable to be recognized as -D-/-D- during routine testings.
    A very rare case of delayed hemolytic transfusion reaction due to anti-Hro antibody in a patient with -D-/-D- was reported.
  • 村田 繁子, 松崎 哲夫, 小林 美智子, 丸山 正雄, 海野 和廣, 田部 正孝, 中嶋 八良
    1987 年 33 巻 6 号 p. 761-765
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    An 18-year-old healthy female blood donor with Jk (a-b-) phenotype was detected in a routine survey by using the test for urea lysis resistance. Her red blood cells showed intermediate resistance between the ordinary Jk (a+b+) and the recessive Jk (a-b-) cells. Furthermore, the absorption-elution test on this proband's cells using anti Jka and anti Jkb sera demonstrated the presence of a weakly expressed Jka antigen. From these findings, we suspected a genetic mechanism different from that of the recessive Jk (a-b-), and carried out a family study. Of her 12 paternal blood relatives and sibs inclusively, ten were identified of their Kidd phenotype quite resembling to that of the proband, whereas her mother and other non-blood relatives showed a normal Kidd phenotype. This finding strongly suggests presence of a non-allelic dominant gene, which might inhibit the normal expression of Kidd antigens and result in a Jk (a-b-) phenotype, as was already proposed by Okubo et al (1986).
  • 圓谷 敏彦, 内野 純一, 岩谷 ユリ子, 堀江 卓, 柿田 章
    1987 年 33 巻 6 号 p. 766-769
    発行日: 1987年
    公開日: 2010/03/12
    ジャーナル フリー
    A case of hemolytic transfusion reaction due to Lewis b antibody was reported.
    The patient, 62-year-old farmer, was suffered from echinococcosis. He has no history to be transfused. His blood types were A, CCDee, Le (a-b-), MNSs, P2, kk, Fy (a+b-), Jk (a+b+), Xg (a+), Di (a-), and he had anti Lea & Leb antibodies.
    Right hepatic lobectomy was undergone. Total amount of bleeding in this operation was about 3, 000ml. And during the operation, 1, 600ml of Le (a-b-) blood and 800ml of Le(a-b+) blood was given.
    Two days after the operation, hemolytic jaundice and bleeding tendency from DIC appeared. Some part of the lost blood volume was replaced by incompatible Le (a-b+) blood, because Le (a-b-) blood was difficult to obtain due to its low frequency. Moreover few papers reported on hemolytic reaction by anti Leb antibody. Hemolytic reaction and DIC syndrome became even stronger as the days go by.
    After the transfusions of Le (a-b-) blood units alone, hemolytic anemia, jaundice and bleeding tendency were improved before long. Anti-Leb antibody titer which elevated initially decreased gradually after stoppage of using Le (b+) blood.
    According to these clinical features and laboratory data, we concluded that this hemolytic reaction was occured from Le (b+) incompatible blood transfusion.
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