日本輸血学会雑誌
Online ISSN : 1883-8383
Print ISSN : 0546-1448
ISSN-L : 0546-1448
41 巻, 1 号
選択された号の論文の6件中1~6を表示しています
  • HLAホモ接合体細胞の輸血後GVHD発症要因に関連して
    稲葉 洋行, 荒木 延夫, 浜中 泰光, 阪田 宣彦, 猪子 英俊, 能勢 義介
    1995 年 41 巻 1 号 p. 1-7
    発行日: 1995年
    公開日: 2010/03/12
    ジャーナル フリー
    The presence of HLA-homozygous typed cell (HTC) in transfused blood cells possibly induces transfusion-associated graft versus host disease (GVHD) which usually causes immediate death for transfusion-associated GVDH in a Japanese population, the frequency of HTC was investigated by serological typing for class I antigens and by DNA typing for class II antigens. Out of 3, 000 Japanese cells randomly collected, cells from 32 Japanese (1.1%) were found to be HLA-homozygous for the HLA-A, -B, -C, DRB1, DQA1 and DQB1 genes. This high frequency suggests that removal or inactivation of leukocytes in donors' blood is necessary to prevent transfusion-associated GVHD.
  • 塚田 理康, 高橋 孝喜, 柴田 洋一
    1995 年 41 巻 1 号 p. 8-15
    発行日: 1995年
    公開日: 2010/03/12
    ジャーナル フリー
    Properties of red blood cells in KL-3R3 (CPDA-1) blood preservation solution were evaluated in vitro and In vivo. The KL-3R3 whole blood were collected from 10 healthy volunteers and stored for 6 weeks at 4 to 6°C.
    In vitro the value of RBC count, Hb, Ht, ATP, 2-3DPG, in red cells red cell shapes and osmotic membrane fragility of red cells, and pH, electrolytes, glucose, and ammonia in the plasma were measured once a week during 6 weeks preservation. In addition, to evaluate the aggregates formation in KL-3R3 whole blood, we compared KL-3R3 whole blood with CPD whole blood on plasma-fibrinogen value during storage and trapped aggregates on transfusion filter membrane (KL-3R3: after 5 weeks, CPD: after 3 weeks). In vivo survival studies of autologous red cells after transfusion were carried out after storage for 5 weeks.
    The changes in vitro were similar to data reported previously in overseas.
    Plasma fibrinogen decreased during storage, but amounts of aggregates on transfusion filter was similar to those of CPD whole blood. The 24- and 48-hours post-transfusion survivals of 51Cr-labeled autologous red cells were 83.8±2.9% and 80.3±3.5% after 35 days storage (n=10), respectively.
    KL-3R3 whole blood could be stored for 35 days with good quality, and allowed high levels of post-transfusion recovery.
  • 吉田 久博, 万木 紀美子, 伊藤 和彦
    1995 年 41 巻 1 号 p. 16-20
    発行日: 1995年
    公開日: 2010/03/12
    ジャーナル フリー
    To evaluate the gel centrifugation method for infectious disease tests, basic experiments were performed with various commercial kits by the hemo- or particle-agglutination method. Optimal conditions for the method were considered to be an incubation time of 15min, an incubation temperature of 24-26°C (room temperature), and a half sensitized-particle concentration.
    The gel method was compared with conventional microplate methods for the utility of infectious disease tests with the patients' specimens. There were no discrepancies in the detection tests of HBs-antigen and HCV-, CMV-, HIV, and HTLV-I-antibodies between the gel method and the microplate method, although the gel system detecting HCV-antibody titers was 25% less sensitive than the microplate method. Three of the 83 specimens showed discrepancy in the HBs-antibody detection. They were weak positive by the gel method but negative by the microplate method. We recognized that the test by the gel centrifugation method was reliable, reproducible, and speedy to obtain the results. The gel method could be used as a routine and emergency test for infectious diseases.
  • 小松 文夫
    1995 年 41 巻 1 号 p. 21-27
    発行日: 1995年
    公開日: 2010/03/12
    ジャーナル フリー
    Bone marrow mononuclear cells (BM) were obtained from allogeneic bone marrow donors. The BM-LAK were generated by culturing of the BM in the medium containing recombinant interleukin-2 for several days. The natural killer (NK) activity of BM against K562 was very low. However, the killer activity of BM-LAK against Raji was very high, and significantly higher than that of the peripheral blood lymphocyte (PBL)-LAK. The killer activity of PBL-LAK decreased shortly after 2 weeks of the culture, whereas the BM-LAK maintained high killer activity for a long period. The BM-LAK demonstrated relatively high percentage of CD4-CD8-CD16+, CD8+CD16+ and CD8+CD11b+ phenotypes, compared to the BM. It is likely that these phenotypes represent active killer cells. BM contained many adherent cells which were monocytes and macrophages. It is necessary to remove these adherent cells from the culture within one week, because these cells produced the suppressive factors against the proliferation of BM-LAK. The high killer activity of BM-LAK is effective for adoptive immunotherapy, and may be useful on the purging of tumor cells in autologous bone marrow transplantation.
  • 藤岡 知昭, 野村 一雄, 岡本 知士, 鈴木 薫, 瀬尾 喜久男, 久保 隆
    1995 年 41 巻 1 号 p. 28-33
    発行日: 1995年
    公開日: 2010/03/12
    ジャーナル フリー
    Predeposit autologous boood transfusion was performed in 40 patients with renal cell carcinoma who underwent radical nephrectomy between January 1989 and June 1994. No homologous blood was transfused in 31 cases (77.5%) of which 27 cases (81.8%) prepared for predeposit autologus bloos ≥600ml. The prognosis of 20 patients with the autologous blood transfusion until December 1991 was compared with that of 25 patients with homologous blood transfusion during the same period. Recurrence-free rate of autologous blood transfused patients was significantly (p<0.05) lower than that of homologous blood transfused cases. There was no significant difference of survival rate among autologous and homologous blood transfused cases. Patients transfused with homologous whole blood had significantly (p<0.05) poorer survival than autologous blood transfused cases, in addition to significantly (p<0.01) higher tumor recurrence.
    These results suggested that autologous blood tranfusion had a potential to improve the prognosis of renal cell carcinoma.
  • 血液製剤について
    三浦 泰裕, 奥山 堅司, 水沢 左衛子, 本間 玲子
    1995 年 41 巻 1 号 p. 34-38
    発行日: 1995年
    公開日: 2010/03/12
    ジャーナル フリー
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