Changes in ATP content and P
50 of the blood during storage play an important role in oxygen transport and red blood cell viability. Three types of stored blood, namely CPD whole blood, CPD-CRC and MAP-CRC, with respect to blood gases, pH and
l-lactate concentration ware examined. The number of days of storage correlated with blood acidity and
l-lactate concentration, and P
50 correlated with pH. On the basis of these observations, the efficacy of high CO
2 permeability bags for blood preservation was tested. Blood P
CO2 remained lower and pH and P
50 higher on storage in these bags than on storage in conventional bags. I also examined the effects of pH change on ATP and 2, 3-DPG levels by addition of
l-lactate to the stored blood. When pH of the blood was higher than 6.9, ATP level decreased significantly. When pH range was lower than 6.8, 2, 3-DPG level decreased. These results suggest that pH between 6.8 and 6.9 is optimal for the simultaneous maintenance of adequate ATP and 2, 3-DPG (and thus also P
50) levels.
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