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Volume 43 (1997)
- Issue 6 Pages 853-
- Issue 5 Pages 665-
- Issue 4 Pages 399-
- Issue 3 Pages 335-
- Issue 2 Pages 175-
- Issue 1 Pages 1-
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Volume 43, Issue 6
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[in Japanese]1997 Volume 43 Issue 6 Pages 853-861
Published: 1997
Released on J-STAGE: March 12, 2010
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SERUM ERYTHROPOIETIN LEVELS FOLLOWING THREE DIFFERENT REPLACEMENT THERAPIES AFTER PHLEBOTOMY IN DOGSAkira Fukui1997 Volume 43 Issue 6 Pages 862-867
Published: 1997
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSWe examined serum erythropoietin (EPO) levels before and after phlebotomy with three different volume replacement methods in dogs. Blood was sampled from each dog at 40ml/kg under general anesthesia.
The dogs were divided into three groups. The first group was treated with homologous transfusion (BLOOD group), the second with 6% hydroxyethyl starch (HES group) and the third with artificial red blood cells (NRC group), at the same volume. EPO levels were measured at 2, 4, 6 hours, and on days 1, 2, 3, 5 and 7 after treatment. EPO levels reached maximum at 6 hours in the BLOOD group, at 2 days in the HES group and at 1 day in the NRC group. Maximum level was highest in the NRC group, followed by the HES and BLOOD groups in descending order. Accompanying the increase in EPO levels, the red blood cell count recovered to normal on days 7 and 5 in the HES and NRC groups, respectively. We conclude that the increase in EPO levels seems to be controlled mainly by two factors: the acute anemic condition which triggers the initial induction, and the duration and degree of production.View full abstractDownload PDF (757K) -
Taiko Seno, Mitsunobu Tanaka, Suguru Akamatsu, Tetsuya Tachikawa, Fumi ...1997 Volume 43 Issue 6 Pages 868-876
Published: 1997
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSA highly sensitive enzyme-linked immunosorbent assay (ELISA) method using H-disaccharide (Fucα1, 2 Galβ-BSA) was developed to measure α (1, 3)-N-acetyl-D-galactosaminyltransferase (A-transferase) and α (1, 3)-D-galactosyltransferase (B-transferase) activities in human plasma. Fifty-eight and 43 plasma samples from various subgroups of A and B, respectively, were assayed with UDP-GalNAc or UDP-Gal in microtiter plate wells coated with H-disaccharide covalently attached to BSA, and the products formed by transferases were detected by horse-radish peroxidase-conjugated anti-A or anti-B monoclonal antibody. Transferase activities measured by this method were compared with those by the conventional method based on the conversion of O-type erythrocytes into A- or B-type erythrocytes.
The present method allowed detection of weak enzyme activities from A- and B-type subgroups and variants such as A2, A3, AXB, cisAB (for A-transferase) and AB3, BX, cisAB (for B-transferase). In contrast, these activities could not be detected at all by the conventional method. Further, enzyme activities of all samples (n=101) except one from AX were quantified by this ELISA method, whereas only 21% (12/58) of the A-transferase and 58% (25/43) of B-transferase from the same individuals were determined to be present by conversion of blood types on erythrocytes.
These results demonstrate that the present method was sufficiently sensitive for the assay of weak A- and B-transferase activities and the typing of A and B blood groups.View full abstractDownload PDF (1023K) -
Naoko Sugano, Yoko Nakagi, Keiko Minamiru, Koji Kishino, Takashi Obata ...1997 Volume 43 Issue 6 Pages 877-882
Published: 1997
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSChanges in anti-A and anti-B antibody titer and A- and B-glicosyltransferase activity were analyzed in five patients after minor ABO-incompatible bone marrow transplantation (BMT). All patients showed complete engraftment after BMT. Shortly after BMT, blood types of recipient's erythrocytes as examined by standard agglutination techniques changed into those of the donor's erythrocytes. Using the elution method, however, recipient's erythrocyte antigens were detected on erythrocytes from three of five patients with complete engraftment after BMT. Analysis of A- and B-transferase activity in serum demonstrated that four of five patients maintained pre-BMT transferase activity. The other patient, who showed no A- or B-transferase activity after BMT, had an inhibitor to this activity. These results suggest that in patients receiving BMT, a small amount of blood antigens on erythrocytes and serum transferase activity may be derived from the patient's tissue.View full abstractDownload PDF (785K) -
Katsuya Morishita, Takako Mitani, Shinichiro Sato, Toshiaki Kato, Tosh ...1997 Volume 43 Issue 6 Pages 883-889
Published: 1997
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSHPA-alloimmunization is a major factor in refractoriness to HLA-matched platelet transfusion. Retrospective studies indicate that although HPA-matched platelet transfusion is effective in such cases, it is extremely difficult to deal with such situations prospectively. We report a case of successful transfusion of HPA-matched platelets selected by HPA-DNA typing with frozen lymphocytes of HLA-matched donors. The patient was a 51-year-old male with CML who developed HLA antibodies after multiple trnasfusion of random platelets. However, HLA-matched platelets were ineffective. Further serological tests including monoclonal antibody-specific immobilization of platelet antigens (MAIPA) revealed that he also possessed HPA-3a antibody, which appeared to be responsible for the refractoriness. HPA-DNA typing by the PCR-SSP method indicated that his HPA-3 type was b/b and those of refractory donors were HPA-3a/a or HPA-3a/b. By HPA-3 typing with 122 frozen lymphocytes of HLA-matched donors, 14 donors were found to be HPA-3b/b. HLA-HPA matched platelets from these donors were shown to be effective, indicating that refractoriness in this patient was due to HPA-3a-alloimmunization. The method described here for selection of HLA-HPA matched donors is effective under the following circumstances: 1) a considerable number of HLA-matched donors and their frozen lymphocytes are available; 2) HPA matching frequency of a given case is not rare; and 3) transfusion of matched platelets is expected to be terminated in a limited period.View full abstractDownload PDF (3039K) -
Mina Komoto, Fumitake Awano, Seiichi Tominaga1997 Volume 43 Issue 6 Pages 890-895
Published: 1997
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSWe performed platelet collection by a dual needle procedure with a Fresenius Blood Cell Separator AS-104 in 21 healthy donors (19 male, 2 female). Collection time was set at 60 minutes using PAIP (Periodically Alternating Interface Position) software, namely the PLT-5d program (Ver. 4. 71). Collected platelet concentrate contained 2.42±0.74×1011 platelets/bag in total. Platelet collection efficiency was very good, at 38.4±5.4% (Table 3). Leukocyte content was as law as 1.21±0.89×105/bag and below 1×106 leukocytes/bag in all platelet concentrates (Fig. 2). AS-104 provided highly effective PLT collection.View full abstractDownload PDF (665K) -
T. Yamaguchi, H. Yasuda, K. Sato, T. Akama, K. Kato, N. Iwabuchi, T. O ...1997 Volume 43 Issue 6 Pages 896-900
Published: 1997
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSWe report a patient who showed delayed hemolytic transfusion reaction (DHTR) twice in two weeks due to multiple antibodies anti-C, -e and -Jka.
The patient was a 67-year-old male with liver cirrhosis and hepatoma. The first DHTR was observed on day 7 after transfusion of 6 units of red cells for bleeding from gastric varices. Levels of both LDH and total bilirubin were remarkably increased and occult hemoglobulin was detected in urine. Anti-C was demonstrated in serum and in the eluate of red cells, however, the direct antigloblin test was negative. On the night of day 9 after the first transfusion, he received 2 additional units of compatible red blood cells (C-negative) crossmatched by the saline and enzyme methods. The second DHTR with hemoglobinuria was observed immediately after transfusion. Anti-e, anti-Jka, and anti-P1 as well as anti-C were later identified in the serum used for cross-matching. Clinical symptoms including blacktea-colored urine and melena transiently occurred but no serious sequelae were seen. In terms of the sensitivity and specificity, the polyethylene glycol antiglobulin test is superior to the albumin antiglobulin test, as the latter detected earlier and easier antibodies to C and JKa observed in this case, whereas the former hardly could do.View full abstractDownload PDF (537K) -
1997 Volume 43 Issue 6 Pages 901-950
Published: 1997
Released on J-STAGE: March 12, 2010
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1997 Volume 43 Issue 6 Pages 951-1002
Published: 1997
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (8164K) -
1997 Volume 43 Issue 6 Pages 1016-1025
Published: 1997
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (2875K)
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