We evaluated the efficacy of two skin disinfectants (10% povidone iodine vs. 0.5% chlorhexidine gluconate) at the donor phlebotomy site against three different species of bacteria (
Bacillus cereus, coagulase-negative staphylococci,
Propionibacterium acnes).
The growth of
Bacillus cereus in apheresis-derived platelet concentrates was very rapid with a doubling time of 6 hours, and reached 10
7CFU/ml within 72 hours. When RC-MAP prepared from whole blood inoculated with
Bacillus cereus was stored at 4°C, growth of
Bacillus cereus was stopped for 21 days. However, when storage temperature was increased to 22°C on Days 7 or 21, rapid proliferation occurred as was observed in apheresis-derived platelets experiments. Bacterial proliferation was also observed in buffy coat and platelet-poor plasma prepared from whole blood.
No difference was seen between 10% povidone iodine and 0.5% chlorhexidine gluconate in the efficacy of disinfectants against coagulase-negative staphylococci and
Propionibacterium acnes. However, disinfection of
Bacillus cereus was achieved with 10% povidone iodine only.
Bacillus cereus showed proliferation in all blood components. These results suggest that donor skin disinfectant should be done using 10% povidone iodine in any blood collection.
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