Journal of the Japan Society of Blood Transfusion
Online ISSN : 1883-8383
Print ISSN : 0546-1448
ISSN-L : 0546-1448
Volume 47, Issue 5
Displaying 1-6 of 6 articles from this issue
  • Dai Sasaki, Satoshi Kosunago, Yoshimitsu Komiyama, Ko Suzuki, Shin-ich ...
    2001 Volume 47 Issue 5 Pages 777-782
    Published: December 01, 2001
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    Washed platelet concentrates (WPC) have been used for patients with severe non-hemolytic febrile transfusion reaction (NHFTR) following ordinary PC transfusion. We studied three synthetic platelet storage solutions, namely Solution 1 containing saline including 0.1% citrate, Solution 2 based on acetate Ringer's solution with glucose and citrate, and Solution 3 being Seto solution designed for longer storage of PC. The pH of PC washed with Solution 1 rapidly decreased from 6.37 to 5.82 after 1 day storage, but remained at 6.8 throughout storage for 5 days with Solutions 2 and 3. On day 5, Pselectin concentrations in the supernatant of PC washed with Solution 3 were still lower than those washed with Solution 1 or Solution 2 and stored for 1 day or 3 days, respectively. The percent hypotonic shock response (%HSR) of Solution 1 was completely lost after 1 day storage, and Solution 2 reached about 20% after 3 day storage. %HSR and discoid shape were maintained at a high level, for 5 days by using Solution 3. These results suggest that Solution 3 is the most desirable solution for platelets. Owing to the rapid deterioration of platelet quality, we recommended that transfusion of WPC prepared with Solution 1 or Solution 2 be done within 6 hr or 1 day, respectively.
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  • Masanobu Yamaoka, Shizuko Inazawa, Kayoko Kodama, Tadaaki Ikeda, Shige ...
    2001 Volume 47 Issue 5 Pages 783-790
    Published: December 01, 2001
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    The subject was a 28-year-old Japanese woman whose red blood cells had two populations of A, CCDee, Jk (a+b-), and AB, CcDEe, Jk (a+b+), at 95% and 5%, respectively. The karyotype for her lymphocytes was a female type of 46, XX, 100%. She was not a twin and had no history of chimeric features such as hermaphroditism, patchy skin pigmentation, or heterochromia iridis, as is often seen in dispermic chimeras. Her chimerism was further investigated using genomic DNA from leukocytes and nails as a somatic sample. Amelogenin genes specific for Y chromosome were not detected in her nails. Analyses of microsatellite and blood group genes were carried out by a PCR-SSCP method. Analysis of ABO gene revealed that A, B, and O gene coexisted in not only her peripheral blood but also her nails, and that the amount of B gene was less than that of A or O gene, corresponding to the rate of red blood cells. Rh blood group gene analyses showed the same result. Furthermore, a double contribution of alleles from her father was suggested both in her peripheral blood and nails by microsatellite analysis at the D1S102 locus. Therefore, on the basis of these findings, we considered this was a case of dispermic chimera.
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  • CPD VS. ACD-A SOLUTION
    Mitsuaki Akino, Hiromi Awakura, Masako Satoh, Sadamitsu Yamamoto, Sato ...
    2001 Volume 47 Issue 5 Pages 791-793
    Published: December 01, 2001
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
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  • Asako Hiraoka, Misae Tanihiro, Rie Masuda, Emi Kurita, Teruhisa Fujii, ...
    2001 Volume 47 Issue 5 Pages 794-795
    Published: December 01, 2001
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
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  • 2001 Volume 47 Issue 5 Pages 811-815
    Published: December 01, 2001
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
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  • 2001 Volume 47 Issue 5 Pages 816-817
    Published: December 01, 2001
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    Download PDF (294K)
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