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Volume 49 (2003)
- Issue 6 Pages 741-
- Issue 5 Pages 633-
- Issue 4 Pages 549-
- Issue 3 Pages 403-
- Issue 2 Pages 113-
- Issue 1 Pages 27-
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Volume 49, Issue 5
Displaying 1-10 of 10 articles from this issue
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Hiroshi Osawa, Shinpei Yoshii, Hiroji Higuchi, Shigeru Hosaka, Shoji S ...2003 Volume 49 Issue 5 Pages 633-639
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (1687K) -
Masateru Sasaki, Mikako Yamada, Reiko Kaneko, Daisuke Furuya, Teruo En ...2003 Volume 49 Issue 5 Pages 640-645
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSIn recent years, new methods such as microtube column agglutination methods and microplate methods using automated instruments have been used for blood typing, antibody screening testing and crossmatching testing. However, no precise comparison study of the performance of these methods and conventional tube methods has been done.
In the present study, we performed a comparison study on sensitivity for IgG alloantibody by the tube indirect anti-globulin test (IAT) using polyethylene glycol (PEG), albumin, or low ionic strength saline solution (LISS), microtube column agglutination (Ortho BioVue and DiaMed MTS), and microplate solid phase system (Immucor Capture R Ready Screen, CRRS) methods.
Using 51 positive sera as determined by the tube IAT-PEG using anti-IgG antibody, the positive rates of tube IAT-albumin and tube IAT-LISS using anti-IgG antibody, BioVue (IgG cassette), MTS (IgG card) and CRRS (IgG bonded RBC) were 62.7%, 68.6%, 88.2%, 88.2% and 94.1%, respectively.
Using 12 commercial anti-sera and 25 patient sera, antibody reactivity as determind by AABB scores was compared between these 6 methods. Mean AABB scores were 51.4 with the tube IAT-PEG, 37.3 with the tube IAT-albumin, 37.4 with the tube IAT-LISS, 51.7 with BioVue, 53.8 with MTS, and 76.5 with CRRS.
These results indicate that the sensitivity of clinically significant red cell alloantibodies was similar or higher with the microtube column agglutination methods and CRRS than with the tube IAT methods.View full abstractDownload PDF (663K) -
Satoshi Yasumura, Kiyohiro Higuchi, Sachiyo Tabata, Junko Michino, Kaz ...2003 Volume 49 Issue 5 Pages 646-652
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSAn IgG-type anti-IgA antibody was detected in 3 of 22 patients who developed blood transfusionrelated side effects in our hospital in 1998. These patients were females, and developed blood transfusion-related side effects during chemotherapy for malignant diseases. None of the patients had IgA deficiency. With regard to the subclass and allotype of the anti-IgA antibody, IgA1 and IgA2m (1) were common among the 3 patients. IgA2m (2) was present in 1 patient. A washed blood preparation (washed red blood cells, washed platelets) was transfused to 1 of the patients. In the remaining 2, a standard non-washed blood preparation was administered. The anti-IgA antibody titer markedly decreased in the patient treated with the washed preparation. In the remaining 2 patients treated with the non-washed blood preparation, in contrast, no decrease in anti-IgA antibody titer was seen. No side effects of blood transfusion occurred in the patient with a marked decrease in anti-IgA antibody titer, whereas in 1 of 2 patients without reduction of anti-IgA antibody titer, 3 adverse events related to blood transfusion were observed. Administration of washed blood preparation to patients with anti-IgA antibody may be required to prevent complications of blood transfusion.View full abstractDownload PDF (744K) -
Shinobu Wakamoto, Mitsuhiro Fujihara, Hideki Abe, Miki Yamaguchi, Shin ...2003 Volume 49 Issue 5 Pages 653-659
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSHemoglobin vesicles (HbV), a type of liposome-encapsulated hemoglobin (LEH), have been recently developed as an artificial oxygen carrier. The efficacy of HbV has been demonstrated in the transfusion of HbV into rodent models of hemorrhagic shock. It is important to evaluate the compatibility of HbV with human blood cells. We examined the effects of HbV on human platelet activation in vitro by estimating the platelet release reaction and expression of platelet surface activation markers in the presence or absence of agonists. HbV concentration in the reaction volume was prepared at 20% or 40%. Preincubation of platelet-rich plasma (PRP) with HbV had no adverse effects on RANTES or serotonin release from platelets. Preincubation of whole blood with HbV also had no effects on exposure of P-selectin on platelets. However, binding of PAC-1, a monoclonal antibody that detects the activation-dependent conformational change of αIIbβ3 to platelets, was amplified by preincubation of whole blood with HbV in the presence of relatively low concentration of ADP. These results suggest that HbV enhances the binding of PAC-1 to platelets. The clinical meaning of this increased binding of PAC-1 needs to be addressed.View full abstractDownload PDF (2029K) -
Yutaka Nagura, Hirokazu Tsuno, Yoichi Shibata, Koki Takahashi2003 Volume 49 Issue 5 Pages 660-665
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSIn the present study, we investigated the effects of radiation and leukocyte-reduction filters on platelet function. Platelet aggregation in response to collagen and ADP were measured prior to and after irradiation and filtration, as were the platelet recovery rate and complement factor C3. Four types of leukocyte-reduction filter were used, namely positively-, negatively-, and non-charged filters (all of polyester composition), as well as a polyurethane filter. Radiation itself did not significantly affect either the platelet recovery rate, platelet function, or C3 value. On the other hand, filtration through polyester leukocyte-reduction filters resulted in a significant reduction in the platelet recovery rate, an effect not observed with the polyurethane filter. However, none of the filters caused significant changes in platelet function or in C3 value. We concluded that radiation and filtration do not cause significant changes in platelet function, but polyurethane filters are superior to polyester filters in relation to platelet recovery.View full abstractDownload PDF (656K) -
COMPARATIVE STUDY OF AUTOVUE SYSTEM AND STANDARD TUBE TESTHiroshi Miyako, Yoshiaki Tachikawa, Chieko Iwao, Yasuhiro Magari, Hiro ...2003 Volume 49 Issue 5 Pages 666-672
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSWe compared the ID-GelStation (GS) with Auto Vue system (AV) equipment and standard tube test (TT) with regard to ABO and Rh blood typing and red cell antibody screening in 190 samples, 59 antibody-positive sera and 71 antibodies. GS showed an equal reaction to TT and AV in ABO cell typing and Rh typing. In ABO reverse typing, GS tended to show s stronger reaction than AV but a weaker reaction than TT, with which 9 of 109 samples needed to retested.
In red cell antibody screening for 71 antibodies, the papain two-stage technique of GS apparently showed stronger reaction than the bromeline one-stage technique, especially for anti-Rh antibody. Further, GS detected antibodies such as anti-Fy, anti-Jk and anti-Jr which were detectable in antiglobulin testing. GS was more sensitive for anti-Rh antibody than AV in antiglobulin testing, the most important in the present blood transfusion tests. However, GS was less sensitive than the polyethylene glycol antiglobulin test (PEG-IgG) of TT. Only 4 of 10 anti-Fy and anti-Jk could be detected, suggesting that GS and AV, in which a low ionic strength solution (LISS) is used, have lower ability to detect low titer antibodies than PEG-IgG. Use of the more sensitive PEG-IgG may be for the detection of some low titer antibodies.View full abstractDownload PDF (2424K) -
Kimiko Yurugi, Shinya Kimura, Hiroaki Tsuji, Norimi Niwa, Ryoko Takega ...2003 Volume 49 Issue 5 Pages 673-677
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSWe commenced a 24-hour blood service in collaboration with staff of the Departments of Laboratory Medicine and Clinical Pathology from April, 2003 in Kyoto University Hospital. Eight months after commencing a 24-hour blood service, we reviewed our experiences by obtaining opinions from technicians of the Departments of Laboratory Medicine and Clinical Pathology. This paper discusses considerations important for establishment of a 24-hour blood service.
While orders for blood testing varied in number during night-duty hours, there were 137 calls-for-help from technicians of the Departments of Laboratory Medicine and Clinical Pathology to those of the Department of Transfusion Medicine from April to December, 2003. Approximately half of these calls concerned problems with the computer system and automated blood testing apparatus, followed by information regarding requests from physicians and blood testing results. Technicians of the Department of Transfusion Medicine were in some cases requested to visit the requesting site, but the percent frequency of calls-for-help per blood testing order gradually decreased. The establishment of a 24-hour blood service resulted in a marked decrease in wasted blood products and provided efficient blood transfusion therapy. However, a number of problems concerning working conditions remain unresolved.View full abstractDownload PDF (708K) -
Takanori Higashitani, Hiroyuki Kawano, Koichi Egashira, Kouji Hashimot ...2003 Volume 49 Issue 5 Pages 678-682
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSBacterial contamination of autologous blood is a major risk factor in autologous blood transfusion. We report here a case of autologous blood transfusion contaminated with Pseudomonas putida and Comamonas acidovorans.
A 69-year-old male had planned to use 1, 200ml of autologous blood during a surgical operation. The first blood donation of 400ml harvested from the patient was sent to the Fukuoka Red Cross Blood Center for separation into red cell concentrate with MAP solution and fresh frozen plasma. These blood components were stored at the Blood Center until use.
Thirty-one days later, transfusion of the red cell concentrate in MAP solution from which coagulated blood had been removed was started in the patient. At 15min, the patient experienced chills and shaking. However, the transfusion was continued, until a high fever of 39.8°C was noted. At that time more than 200ml of autologous blood had been transfused. This blood was confirmed to be contaminated with P. putida and C. acidovorans., and showed a high concentration of endotoxin. The patient recovered well after appropriate treatment and eventually underwent successful a coronary artery bypass grafting.View full abstractDownload PDF (1911K) -
2003 Volume 49 Issue 5 Pages 685-722
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (5948K) -
2003 Volume 49 Issue 5 Pages 723-724
Published: November 01, 2003
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSDownload PDF (136K)
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